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Protein

Carbamoyl-phosphate synthase small chain

Gene

carA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei268NucleophileUniRule annotation1
Active sitei351UniRule annotation1
Active sitei353UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase small chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chainUniRule annotation
Gene namesi
Name:carAUniRule annotation
Ordered Locus Names:HP_1237
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001122821 – 375Carbamoyl-phosphate synthase small chainAdd BLAST375

Proteomic databases

PaxDbiO25835.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotation

Protein-protein interaction databases

DIPiDIP-3716N.
IntActiO25835. 2 interactors.
MINTiMINT-179287.
STRINGi85962.HP1237.

Structurei

3D structure databases

ProteinModelPortaliO25835.
SMRiO25835.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini188 – 375Glutamine amidotransferase type-1UniRule annotationAdd BLAST188

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 184CPSaseAdd BLAST184

Sequence similaritiesi

Belongs to the CarA family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105C1M. Bacteria.
COG0505. LUCA.
KOiK01956.
OMAiCFNTGMT.

Family and domain databases

CDDicd01744. GATase1_CPSase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiView protein in InterPro
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR035686. CPSase_GATase1.
IPR017926. GATASE.
PfamiView protein in Pfam
PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
SMARTiView protein in SMART
SM01097. CPSase_sm_chain. 1 hit.
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

O25835-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSLYLENGL FLQAQSFGAS GTQAGELVFN TSMSGYQEVI SDPSYKGQFV
60 70 80 90 100
VFSMPEIGVV GANSKDDESF FSCAGVLARH YNEFFSNSRA DFSLSAYLKE
110 120 130 140 150
RGVLGVCGVD TRSLIKTLRH HGCLMMVAST IEHDKNKLEE ILKNAPKISH
160 170 180 190 200
SPLVSSVSTP KITTHQRATF DFKTLDYKPF DEKTSHKIIA VLDFGAKGNI
210 220 230 240 250
LNELQNVGLK ALIYPHHTKA SELIKAYEKK EISGIFLSNG PGDPLSLQQE
260 270 280 290 300
IGEIKQLINA KIPMLGICLG HQLLSIAQGY PTYKLKFGHH GSNHPVKNLK
310 320 330 340 350
TNAVEITAQN HNYCVPEDIE EIAIITHRNL FDNTIEGVRY KNAPIISVQH
360 370
HPESSPGPKE SHYIFKEFVE LLKDF
Length:375
Mass (Da):41,636
Last modified:January 1, 1998 - v1
Checksum:iD7146CA079AB7C1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08282.1.
PIRiE64674.
RefSeqiNP_208029.1. NC_000915.1.
WP_000254171.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08282; AAD08282; HP_1237.
GeneIDi899987.
KEGGiheo:C694_06385.
hpy:HP1237.
PATRICifig|85962.47.peg.1325.

Similar proteinsi

Entry informationi

Entry nameiCARA_HELPY
AccessioniPrimary (citable) accession number: O25835
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: January 1, 1998
Last modified: August 30, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families