Reviewed, UniProtKB/Swiss-Prot O25827 (AK_HELPY)

Names and origin

Protein names

Recommended name:
    Aspartokinase
    EC=2.7.2.4
Alternative name(s):
    Aspartate kinase

Gene names

Name:	lysC
Ordered Locus Names:	HP_1229

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Aspartokinase		PRO_0000066677
Regions
	Domain	271 – 337	67	ACT 1
	Domain	344 – 400	57	ACT 2

Sequences

	Sequence		Length	Mass (Da)
	O25827-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 74723D671FF49395	FASTA	405	44,134
	10 20 30 40 50 60 MLIVQKYGGT SMGSIERIHN VAQRVLESVT LGHQVVVVVS AMSGETDRLL EFGKNFSHNP 70 80 90 100 110 120 NKREMDRIVS VGELVSSAAL SMALERYGHR AISLSGKEAG ILTSSHFQNA VIQSIDTKRI 130 140 150 160 170 180 TELLEKNYIV VIAGFQGADI QGETTTLGRG GSDLSAVALA GALKAHLCEI YTDVDGVYTT 190 200 210 220 230 240 DPRIEEKAQK IAQISYDEML ELASMGAKVL LNRSVELAKK LSVKLVTRNS FNHSEGTLIV 250 260 270 280 290 300 AEKDFKGERM ETPIVSGIAL DKNQARVSME GVEDRPGIAA EIFGALAEYR INVDMIVQTI 310 320 330 340 350 360 GRDGKTDLDF TIVKTQIEET KQALKPFLAQ MDSIDYDENI AKVSIVGVGM KSHSGVASIA 370 380 390 400 FKALAKDNIN IMMISTSEIK ISVLIDIKYA ELAVRTLHAV YQLDQ

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD08274.1.
PIR	E64673.
RefSeq	NP_208021.1.
3D structure databases
SMR	O25827. Positions 3-401.
ModBase	Search...
Genome annotation databases
GeneID	899013.
GenomeReviews	Gene locus HP_1229 in contig AE000511_GR.
KEGG	hpy:HP1229.
NMPDR	fig|85962.1.peg.1217.
TIGR	HP_1229.
Phylogenomic databases
HOGENOM	HBG724395.
OMA	ITGFQGI.
Enzyme and pathway databases
BRENDA	2.7.2.4. 1131.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005260. Asp_kin_monofn. IPR001341. Asp_kinase_dom. IPR018042. Aspartate_kinase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00656. asp_kin_monofn. 1 hit. TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK_HELPY

Accession

Primary (citable) accession number: O25827

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)