O25766 (TRMD_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	HP_1148

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP-Rule MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine(37)-N(1))-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	tRNA (guanine-N(1)-)-methyltransferase HAMAP-Rule MF_00605		PRO_0000060388
Regions
	Region	129 – 134	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	109	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O25766 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 2D7135818A13D822	FASTA	229	25,879
	10 20 30 40 50 60 MKFSVLTLFP QLVWPYFEDS ILKRALEKNL FELEVLNLRD FSANKYQKAD HTLIGGGAGQ 70 80 90 100 110 120 ILDPEMVENA LHSVKNPKHT IFLSAVGKPF KQTDAMRLAQ KKHVVLVCGR YEGFDERSIE 130 140 150 160 170 180 LGADEVFCIG DFILTGGELG ALCLIDSIAR HIQGVLGNAQ SLENESFENH YLEAPNFANA 190 200 210 220 VFKSKEINKI PAPLEYSKGN HAKIKQLKLD LSKLRTKFYR LDLFKQHKS

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD08192.1.
PIR	D64663.
RefSeq	NP_207939.1. NC_000915.1. YP_006935064.1. NC_018939.1.
3D structure databases
ProteinModelPortal	O25766.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3681N.
MINT	MINT-179982.
STRING	85962.HP1148.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD08192; AAD08192; HP_1148.
GeneID	13870343. 899686.
KEGG	heo:C694_05930. hpy:HP1148.
PATRIC	20593647. VBIHelPyl33062_1202.
Phylogenomic databases
eggNOG	COG0336.
KO	K00554.
OMA	PRLLMSP.
ProtClustDB	PRK00026.
Family and domain databases
Gene3D	1.10.1270.20. 1 hit.
HAMAP	MF_00605. TrmD.
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_HELPY

Accession

Primary (citable) accession number: O25766

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program