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O25766 (TRMD_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase

EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:HP_1148
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length229 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP-Rule MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_00605

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00605

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine(37)-N(1))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 229229tRNA (guanine-N(1)-)-methyltransferase HAMAP-Rule MF_00605
PRO_0000060388

Regions

Region129 – 1346S-adenosyl-L-methionine binding By similarity

Sites

Binding site1091S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
O25766 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 2D7135818A13D822

FASTA22925,879
        10         20         30         40         50         60 
MKFSVLTLFP QLVWPYFEDS ILKRALEKNL FELEVLNLRD FSANKYQKAD HTLIGGGAGQ 

        70         80         90        100        110        120 
ILDPEMVENA LHSVKNPKHT IFLSAVGKPF KQTDAMRLAQ KKHVVLVCGR YEGFDERSIE 

       130        140        150        160        170        180 
LGADEVFCIG DFILTGGELG ALCLIDSIAR HIQGVLGNAQ SLENESFENH YLEAPNFANA 

       190        200        210        220 
VFKSKEINKI PAPLEYSKGN HAKIKQLKLD LSKLRTKFYR LDLFKQHKS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD08192.1.
PIRD64663.
RefSeqNP_207939.1. NC_000915.1.
YP_006935064.1. NC_018939.1.

3D structure databases

ProteinModelPortalO25766.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-3681N.
IntActO25766. 1 interaction.
MINTMINT-179982.
STRING85962.HP1148.

Proteomic databases

PRIDEO25766.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD08192; AAD08192; HP_1148.
GeneID13870343.
899686.
KEGGheo:C694_05930.
hpy:HP1148.
PATRIC20593647. VBIHelPyl33062_1202.

Phylogenomic databases

eggNOGCOG0336.
KOK00554.
OMACGRFEGI.
OrthoDBEOG6J48RZ.
ProtClustDBPRK00026.

Enzyme and pathway databases

BioCycHPY:HP1148-MONOMER.

Family and domain databases

Gene3D1.10.1270.20. 1 hit.
HAMAPMF_00605. TrmD.
InterProIPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMsTIGR00088. trmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_HELPY
AccessionPrimary (citable) accession number: O25766
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names