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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates guanosine-37 in various tRNAs.By similarity

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091S-adenosyl-L-methionine; via amide nitrogenBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciHPY:HP1148-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferase (EC:2.1.1.228)
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene namesi
Name:trmD
Ordered Locus Names:HP_1148
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 229229tRNA (guanine-N(1)-)-methyltransferasePRO_0000060388Add
BLAST

Proteomic databases

PRIDEiO25766.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

DIPiDIP-3681N.
IntActiO25766. 4 interactions.
MINTiMINT-179982.
STRINGi85962.HP1148.

Structurei

3D structure databases

ProteinModelPortaliO25766.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1346S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.Curated

Phylogenomic databases

eggNOGiCOG0336.
KOiK00554.
OMAiALCLIDS.
OrthoDBiEOG6J48RZ.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.

Sequencei

Sequence statusi: Complete.

O25766-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFSVLTLFP QLVWPYFEDS ILKRALEKNL FELEVLNLRD FSANKYQKAD
60 70 80 90 100
HTLIGGGAGQ ILDPEMVENA LHSVKNPKHT IFLSAVGKPF KQTDAMRLAQ
110 120 130 140 150
KKHVVLVCGR YEGFDERSIE LGADEVFCIG DFILTGGELG ALCLIDSIAR
160 170 180 190 200
HIQGVLGNAQ SLENESFENH YLEAPNFANA VFKSKEINKI PAPLEYSKGN
210 220
HAKIKQLKLD LSKLRTKFYR LDLFKQHKS
Length:229
Mass (Da):25,879
Last modified:January 1, 1998 - v1
Checksum:i2D7135818A13D822
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08192.1.
PIRiD64663.
RefSeqiNP_207939.1. NC_000915.1.
WP_000672977.1. NC_018939.1.
YP_006935064.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08192; AAD08192; HP_1148.
GeneIDi899686.
KEGGiheo:C694_05930.
hpy:HP1148.
PATRICi20593647. VBIHelPyl33062_1202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08192.1.
PIRiD64663.
RefSeqiNP_207939.1. NC_000915.1.
WP_000672977.1. NC_018939.1.
YP_006935064.1. NC_018939.1.

3D structure databases

ProteinModelPortaliO25766.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3681N.
IntActiO25766. 4 interactions.
MINTiMINT-179982.
STRINGi85962.HP1148.

Proteomic databases

PRIDEiO25766.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08192; AAD08192; HP_1148.
GeneIDi899686.
KEGGiheo:C694_05930.
hpy:HP1148.
PATRICi20593647. VBIHelPyl33062_1202.

Phylogenomic databases

eggNOGiCOG0336.
KOiK00554.
OMAiALCLIDS.
OrthoDBiEOG6J48RZ.

Enzyme and pathway databases

BioCyciHPY:HP1148-MONOMER.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiTRMD_HELPY
AccessioniPrimary (citable) accession number: O25766
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 27, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.