O25728 (HCPC_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative beta-lactamase HcpC
EC=3.5.2.6

Alternative name(s):
Cysteine-rich protein C

Gene names

Name:	hcpC
Ordered Locus Names:	HP_1098

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May hydrolyze 6-aminopenicillinic acid and 7-aminocephalosporanic acid (ACA) derivatives By similarity.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Secreted Potential.
Miscellaneous	Antibodies against HcpC are present in sera from human patients infected by Helicobacter pylori.
Sequence similarities	Belongs to the hcp beta-lactamase family. Contains 7 TPR repeats.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Secreted
Domain	Repeat Signal TPR repeat
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Potential

Chain

26 – 290

265

Putative beta-lactamase HcpC

PRO_0000013196

Regions

Repeat

29 – 62

34

TPR 1

Repeat

64 – 98

35

TPR 2

Repeat

100 – 133

34

TPR 3

Repeat

134 – 170

37

TPR 4

Repeat

172 – 205

34

TPR 5

Repeat

206 – 242

37

TPR 6

Repeat

244 – 278

35

TPR 7

Amino acid modifications

Disulfide bond

56 ↔ 64

Ref.4

Disulfide bond

92 ↔ 100

Ref.4

Disulfide bond

128 ↔ 136

Ref.4

Disulfide bond

164 ↔ 172

Ref.4

Disulfide bond

200 ↔ 208

Ref.4

Disulfide bond

236 ↔ 244

Ref.4

Disulfide bond

272 ↔ 280

Ref.4

Secondary structure

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290

Helix Strand Turn

Details...

Helix

29 – 41

13

Helix

45 – 57

13

Helix

61 – 73

13

Beta strand

75 – 77

3

Helix

81 – 93

13

Helix

97 – 109

13

Beta strand

111 – 113

3

Helix

117 – 129

13

Helix

133 – 145

13

Beta strand

147 – 149

3

Helix

153 – 165

13

Helix

169 – 181

13

Beta strand

183 – 185

3

Helix

189 – 201

13

Helix

205 – 217

13

Helix

225 – 237

13

Helix

241 – 252

12

Beta strand

255 – 258

4

Helix

264 – 274

11

Helix

277 – 284

8

Sequences

	Sequence		Length	Mass (Da)
	O25728 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 0F0CFD779A6F108E	FASTA	290	31,594
	10 20 30 40 50 60 MLENVKKSFF RVLCLGALCL GGLMAEQDPK ELVGLGAKSY KEKDFTQAKK YFEKACDLKE 70 80 90 100 110 120 NSGCFNLGVL YYQGQGVEKN LKKAASFYAK ACDLNYSNGC HLLGNLYYSG QGVSQNTNKA 130 140 150 160 170 180 LQYYSKACDL KYAEGCASLG GIYHDGKVVT RDFKKAVEYF TKACDLNDGD GCTILGSLYD 190 200 210 220 230 240 AGRGTPKDLK KALASYDKAC DLKDSPGCFN AGNMYHHGEG ATKNFKEALA RYSKACELEN 250 260 270 280 290 GGGCFNLGAM QYNGEGVTRN EKQAIENFKK GCKLGAKGAC DILKQLKIKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[2]	"Immunoproteomics of Helicobacter pylori infection and relation to gastric disease." Haas G., Karaali G., Ebermayer K., Metzger W.G., Lamer S., Zimny-Arndt U., Diescher S., Goebel U.B., Vogt K., Roznowski A.B., Wiedenmann B.J., Meyer T.F., Aebischer T., Jungblut P.R. Proteomics 2:313-324(2002) [PubMed] [Europe PMC] [Abstract] Cited for: ANTIGENICITY.
[3]	"Detection of high titers of antibody against Helicobacter cysteine-rich proteins A, B, C, and E in Helicobacter pylori-infected individuals." Mittl P.R.E., Luethy L., Reinhardt C., Joller H. Clin. Diagn. Lab. Immunol. 10:542-545(2003) [PubMed] [Europe PMC] [Abstract] Cited for: ANTIGENICITY.
[4]	"The crystal structure of Helicobacter cysteine-rich protein C at 2.0 A resolution: similar peptide-binding sites in TPR and SEL1-like repeat proteins." Luethy L., Grutter M.G., Mittl P.R.E. J. Mol. Biol. 340:829-841(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 24-290, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000511 Genomic DNA. Translation: AAD08141.1.

PIR

B64657.

RefSeq

NP_207889.1. NC_000915.1.
YP_006935013.1. NC_018939.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OUV	X-ray	2.00	A	24-290	[»]

ProteinModelPortal

O25728.

SMR

O25728. Positions 28-290.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-3502N.

MINT

MINT-164700.

STRING

85962.HP1098.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAD08141; AAD08141; HP_1098.

GeneID

13870291.
899634.

KEGG

heo:C694_05665.
hpy:HP1098.

PATRIC

20593535. VBIHelPyl33062_1147.

Phylogenomic databases

eggNOG

COG0790.

KO

K07126.

OMA

YHHGEGA.

ProtClustDB

CLSK495918.

Family and domain databases

Gene3D

1.25.40.10. 1 hit.

InterPro

IPR006597. Sel1-like.
IPR011990. TPR-like_helical.
IPR013105. TPR_2.
IPR019734. TPR_repeat.
[Graphical view]

Pfam

PF08238. Sel1. 6 hits.
PF07719. TPR_2. 1 hit.
[Graphical view]

SMART

SM00671. SEL1. 7 hits.
[Graphical view]

PROSITE

PS50005. TPR. 1 hit.
PS50293. TPR_REGION. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O25728.

Entry information

Entry name

HCPC_HELPY

Accession

Primary (citable) accession number: O25728

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program