O25722 (FTSK_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA translocase FtsK

Gene names

Name:	ftsK
Ordered Locus Names:	HP_1090

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	858 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif By similarity.
Subunit structure	Homohexamer. Forms a ring that surrounds DNA By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity. Note: Located at the septum By similarity.
Domain	Consists of an N-terminal domain, which is sufficient for the localization to the septal ring and is required for cell division, followed by a linker domain, and a C-terminal domain, which forms the translocation motor involved in chromosome segregation. The C-terminal domain can be further subdivided into alpha, beta and gamma subdomains. The alpha and beta subdomains multimerise to produce a hexameric ring, contain the nucleotide binding motif and form the DNA pump. The gamma subdomain is a regulatory subdomain that controls translocation of DNA by recognition of KOPS motifs and interacts with XerD recombinase By similarity.
Sequence similarities	Belongs to the FtsK/SpoIIIE/SftA family. Contains 1 FtsK domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 858	858	DNA translocase FtsK		PRO_0000098262
Regions
	Transmembrane	9 – 29	21	Helical; Potential
	Transmembrane	48 – 68	21	Helical; Potential
	Transmembrane	74 – 94	21	Helical; Potential
	Transmembrane	116 – 136	21	Helical; Potential
	Topological domain	137 – 858	722	Cytoplasmic Potential
	Domain	532 – 722	191	FtsK
	Nucleotide binding	552 – 557	6	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O25722 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 273C6384DB36B377	FASTA	858	96,445
	10 20 30 40 50 60 MQPMKSKKLY LALIIGVLLA FLTLSSWLGN SGLVGRFGVW FAALNKKYFG HLSFINLPYL 70 80 90 100 110 120 AWVLFLLYKT KNPFTEIVLE KTLGHLLGIL SLLFLQSSLL NQGEIGNSAR LFLRPFIGDF 130 140 150 160 170 180 GLYALITLMV VISYLILFKL PPKSVFYPYM NKTQNLLKEI YKQCLQAFSP NFSPKKEGFE 190 200 210 220 230 240 NTPSDIQKKE TKNDKEKENR KENPINENHK TPNEEPFLAI PTPYNTTLND SEPQEGLVQI 250 260 270 280 290 300 SSHPPTHYTI YPKRNRFDDL TNPTNPPLKE IKQETKEREP TPTKETLTPT TPKPIMPTLA 310 320 330 340 350 360 PIIENDNKTE NQKTPNHPKK EENPQENTQE EMIEGRIEEM IKENLKKEEK EVQNAPNFSP 370 380 390 400 410 420 VTPTSAKKPV MVKELSENKE ILDGLDYGEV QKPKDYELPT TQLLNAVCLK DTSLDENEID 430 440 450 460 470 480 QKIQDLLSKL RTFKIDGDII RTYSGPIVTT FEFRPAPNVK VSRILGLSDD LAMTLCAESI 490 500 510 520 530 540 RIQAPIKGKD VVGIEIPNSQ SQIIYLREIL ESELFQKSSS PLTLALGKDI VGNPFITDLK 550 560 570 580 590 600 KLPHLLIAGT TGSGKSVGVN AMILSLLYKN PPDQLKLVMI DPKMVEFSIY ADIPHLLTPI 610 620 630 640 650 660 ITDPKKAIGA LQSVAKEMER RYSLMSEYKV KTIDSYNEQA PSNGVEAFPY LIVVIDELAD 670 680 690 700 710 720 LMMTGGKEAE FPIARIAQMG RASGLHLIVA TQRPSVDVVT GLIKTNLPSR VSFRVGTKID 730 740 750 760 770 780 SKVILDTDGA QSLLGRGDML FTPPGANGLV RLHAPFATED EIKKIVDFIK AQKEVQYDKD 790 800 810 820 830 840 FLLEESRMPL DTPNYQGDDI LERAKAVILE KKITSTSFLQ RQLKIGYNQA ATITDELEAQ 850 GFLSPRNAKG NREILQNF

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD08132.1.
PIR	B64656.
RefSeq	NP_207881.1. NC_000915.1.
3D structure databases
ProteinModelPortal	O25722.
ModBase	Search...
Protein-protein interaction databases
STRING	85962.HP1090.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD08132; AAD08132; HP_1090.
GeneID	899626.
KEGG	hpy:HP1090.
PATRIC	20593519. VBIHelPyl33062_1139.
Phylogenomic databases
eggNOG	COG1674.
KO	K03466.
OMA	CAESIRI.
ProtClustDB	CLSK496006.
Family and domain databases
InterPro	IPR002543. Cell_div_FtsK/SpoIIIE. IPR018541. DNA_translocase_Ftsk_gamma. [Graphical view]
Pfam	PF09397. Ftsk_gamma. 1 hit. PF01580. FtsK_SpoIIIE. 1 hit. [Graphical view]
SMART	SM00843. Ftsk_gamma. 1 hit. [Graphical view]
PROSITE	PS50901. FTSK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTSK_HELPY

Accession

Primary (citable) accession number: O25722

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2003
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program