Reviewed, UniProtKB/Swiss-Prot O25686 (ACSA_HELPY)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase
    Acyl-activating enzyme

Gene names

Name:	acsA
Synonyms:	acs
Ordered Locus Names:	HP_1045

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	662 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Miscellaneous	There is no ortholog for acsA in strain J99. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 662	662	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_0000208365
Sites
	Active site	527	1	By similarity
Amino acid modifications
	Modified residue	623	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O25686-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A83E0F9A34F021F3	FASTA	662	74,948
	10 20 30 40 50 60 MQLDEDLEFA KKIFNPNRAF AKQARIKNMC EYKDLVHEAN EDYEHFWGDL AKQKLTWFKP 70 80 90 100 110 120 FDKVLNSDNA PFFKWFENGK INVSYNCIDR HLKDKKNKVA IIFEGEMGDY NVITYRKLHS 130 140 150 160 170 180 EVNKTANLLK NEFNVKKGDR VIIYMPMIVE SVYMMLACTR IGAIHSIVFA GFSPEALRDR 190 200 210 220 230 240 INDAQAKLVI TADGTFRKGK PYMLKPALDK ALENNACPSV EKALIVIRNA KEIDYVRGRD 250 260 270 280 290 300 FVYNEMVNYQ SDKCEPEMMD SEDPLFLLYT SGSTGKPKGV QHSSAGYLLW AQMTMEWVFD 310 320 330 340 350 360 IRDNDNFWCT ADIGWITGHT YVVYGPLACG ATTLILEGTM SYPDYGRWWR MIEEYRVDKF 370 380 390 400 410 420 YTSPTAIRML HAKGENEPSK YNLESLKVLG TVGEPINPTA WKWFYEKIGN SKCSIVDTWW 430 440 450 460 470 480 QTETGGHIIS PLPGATPIRA SCATLPLPGI HAEVLNEDGT KTKPGEQGFL CITKPWPSMI 490 500 510 520 530 540 RNIWGDEKRY IDSYFSQIKL NGEYVYLSGD GAIVDENGYI TIIGRTDDIV NVSGHRIGTA 550 560 570 580 590 600 EVESAISKHE MVAECAVVGI PDAIKGEGLF AFVVLCDGAK CNLGESLELL KEMNHILSIE 610 620 630 640 650 660 IGKIAKLDNV MYVPGLPKTR SGKIMRRLLK SIAKKEPITQ DLSTLEDVNV VKEIMSIAQM EE

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD08090.1.
PIR	E64650.
RefSeq	NP_207835.1.
3D structure databases
SMR	O25686. Positions 17-654.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3168N.
Genome annotation databases
GeneID	899580.
GenomeReviews	Gene locus HP_1045 in contig AE000511_GR.
KEGG	hpy:HP1045.
NMPDR	fig|85962.1.peg.1032.
TIGR	HP_1045.
Phylogenomic databases
HOGENOM	HBG547964.
OMA	HQRMVDT.
Enzyme and pathway databases
BRENDA	6.2.1.1. 1131.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_HELPY

Accession

Primary (citable) accession number: O25686

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)