Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei317 – 3171Coenzyme AUniRule annotation
Binding sitei510 – 5101ATPUniRule annotation
Binding sitei525 – 5251ATPUniRule annotation
Binding sitei533 – 5331Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei536 – 5361ATPUniRule annotation
Metal bindingi549 – 5491Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi552 – 5521Magnesium; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi393 – 3953ATPUniRule annotation
Nucleotide bindingi417 – 4226ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Synonyms:acs
Ordered Locus Names:HP_1045
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662Acetyl-coenzyme A synthetasePRO_0000208365Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei623 – 6231N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO25686.
PRIDEiO25686.

Interactioni

Protein-protein interaction databases

DIPiDIP-3168N.
IntActiO25686. 7 interactions.
MINTiMINT-164557.
STRINGi85962.HP1045.

Structurei

3D structure databases

ProteinModelPortaliO25686.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 2004Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IQF. Bacteria.
COG0365. LUCA.
KOiK01895.
OMAiAISKHEM.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25686-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLDEDLEFA KKIFNPNRAF AKQARIKNMC EYKDLVHEAN EDYEHFWGDL
60 70 80 90 100
AKQKLTWFKP FDKVLNSDNA PFFKWFENGK INVSYNCIDR HLKDKKNKVA
110 120 130 140 150
IIFEGEMGDY NVITYRKLHS EVNKTANLLK NEFNVKKGDR VIIYMPMIVE
160 170 180 190 200
SVYMMLACTR IGAIHSIVFA GFSPEALRDR INDAQAKLVI TADGTFRKGK
210 220 230 240 250
PYMLKPALDK ALENNACPSV EKALIVIRNA KEIDYVRGRD FVYNEMVNYQ
260 270 280 290 300
SDKCEPEMMD SEDPLFLLYT SGSTGKPKGV QHSSAGYLLW AQMTMEWVFD
310 320 330 340 350
IRDNDNFWCT ADIGWITGHT YVVYGPLACG ATTLILEGTM SYPDYGRWWR
360 370 380 390 400
MIEEYRVDKF YTSPTAIRML HAKGENEPSK YNLESLKVLG TVGEPINPTA
410 420 430 440 450
WKWFYEKIGN SKCSIVDTWW QTETGGHIIS PLPGATPIRA SCATLPLPGI
460 470 480 490 500
HAEVLNEDGT KTKPGEQGFL CITKPWPSMI RNIWGDEKRY IDSYFSQIKL
510 520 530 540 550
NGEYVYLSGD GAIVDENGYI TIIGRTDDIV NVSGHRIGTA EVESAISKHE
560 570 580 590 600
MVAECAVVGI PDAIKGEGLF AFVVLCDGAK CNLGESLELL KEMNHILSIE
610 620 630 640 650
IGKIAKLDNV MYVPGLPKTR SGKIMRRLLK SIAKKEPITQ DLSTLEDVNV
660
VKEIMSIAQM EE
Length:662
Mass (Da):74,948
Last modified:January 1, 1998 - v1
Checksum:iA83E0F9A34F021F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08090.1.
PIRiE64650.
RefSeqiNP_207835.1. NC_000915.1.
WP_001175410.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08090; AAD08090; HP_1045.
GeneIDi899580.
KEGGiheo:C694_05405.
hpy:HP1045.
PATRICi20593427. VBIHelPyl33062_1094.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08090.1.
PIRiE64650.
RefSeqiNP_207835.1. NC_000915.1.
WP_001175410.1. NC_018939.1.

3D structure databases

ProteinModelPortaliO25686.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3168N.
IntActiO25686. 7 interactions.
MINTiMINT-164557.
STRINGi85962.HP1045.

Proteomic databases

PaxDbiO25686.
PRIDEiO25686.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD08090; AAD08090; HP_1045.
GeneIDi899580.
KEGGiheo:C694_05405.
hpy:HP1045.
PATRICi20593427. VBIHelPyl33062_1094.

Phylogenomic databases

eggNOGiENOG4108IQF. Bacteria.
COG0365. LUCA.
KOiK01895.
OMAiAISKHEM.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR032387. ACAS_N.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiACSA_HELPY
AccessioniPrimary (citable) accession number: O25686
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

There is no ortholog for acsA in strain J99.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.