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O25655 (PYRD_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydroorotate dehydrogenase (quinone)

EC=1.3.5.2
Alternative name(s):
DHOdehase
Short name=DHOD
Short name=DHODase
Dihydroorotate oxidase
Gene names
Name:pyrD
Ordered Locus Names:HP_1011
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor By similarity. HAMAP-Rule MF_00225

Catalytic activity

(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP-Rule MF_00225

Cofactor

Binds 1 FMN per subunit By similarity. HAMAP-Rule MF_00225

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP-Rule MF_00225

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00225

Subcellular location

Cell membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00225.

Sequence similarities

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   Cellular componentCell membrane
Membrane
   LigandFlavoprotein
FMN
   Molecular functionOxidoreductase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_process'de novo' UMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

'de novo' pyrimidine nucleobase biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondihydroorotate oxidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351Dihydroorotate dehydrogenase (quinone) HAMAP-Rule MF_00225
PRO_0000148445

Regions

Nucleotide binding67 – 715FMN By similarity
Nucleotide binding312 – 3132FMN By similarity
Region116 – 1205Substrate binding By similarity
Region243 – 2442Substrate binding By similarity

Sites

Active site1811Nucleophile By similarity
Binding site711Substrate By similarity
Binding site911FMN; via amide nitrogen By similarity
Binding site1451FMN By similarity
Binding site1781FMN By similarity
Binding site1781Substrate By similarity
Binding site1831Substrate By similarity
Binding site2141FMN By similarity
Binding site2421FMN; via carbonyl oxygen By similarity
Binding site2621FMN; via amide nitrogen By similarity
Binding site2911FMN; via amide nitrogen By similarity

Secondary structure

........................................................... 351
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O25655 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 784D32A23A36F20C

FASTA35139,002
        10         20         30         40         50         60 
MLYSLVKKYL FSLDAEDAHE KVCKILRMLS SSPFLCNLID SQWGYQNPKL ENEILGLHFP 

        70         80         90        100        110        120 
NPLGLAAGFD KNASMLRALM AFGFGYLEAG TLTNEAQVGN ERPRLFRHIE EESLQNAMGF 

       130        140        150        160        170        180 
NNYGAILGVR SFKRFAPYKT PIGINLGKNK HIEQAHALED YKAVLSKCLN IGDYYTFNLS 

       190        200        210        220        230        240 
SPNTPNLRDL QNKAFVHELF CMAKEMTHKP LFLKIAPDLE TDDMLEIVNS AIGAGAHGII 

       250        260        270        280        290        300 
ATNTTIDKSL VFAPKEMGGL SGKCLTKKSR EIFKELAKAF FNKSVLVSVG GISDAKEAYE 

       310        320        330        340        350 
RIKMGASLLQ IYSAFIYNGP NLCQNILKDL VKLLQKDGFL SVKEAIGADL R 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD08055.1.
PIRC64646.
RefSeqNP_207801.1. NC_000915.1.
YP_006934927.1. NC_018939.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1LX3model-A1-351[»]
ProteinModelPortalO25655.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING85962.HP1011.

Proteomic databases

PRIDEO25655.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD08055; AAD08055; HP_1011.
GeneID13870204.
899546.
KEGGheo:C694_05235.
hpy:HP1011.
PATRIC20593357. VBIHelPyl33062_1059.

Phylogenomic databases

eggNOGCOG0167.
KOK00254.
OMAVYEGPGL.
OrthoDBEOG65BDN8.
ProtClustDBPRK05286.

Enzyme and pathway databases

BioCycHPY:HP1011-MONOMER.
UniPathwayUPA00070; UER00946.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00225. DHO_dh_type2.
InterProIPR013785. Aldolase_TIM.
IPR012135. Dihydroorotate_DH_1_2.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsTIGR01036. pyrD_sub2. 1 hit.
PROSITEPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRD_HELPY
AccessionPrimary (citable) accession number: O25655
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names