Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O25655

- PYRD_HELPY

UniProt

O25655 - PYRD_HELPY

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Dihydroorotate dehydrogenase (quinone)

Gene

pyrD

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.By similarity

Catalytic activityi

(S)-dihydroorotate + a quinone = orotate + a quinol.

Cofactori

FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711SubstrateBy similarity
Binding sitei91 – 911FMN; via amide nitrogenBy similarity
Binding sitei145 – 1451FMNBy similarity
Binding sitei178 – 1781FMNBy similarity
Binding sitei178 – 1781SubstrateBy similarity
Active sitei181 – 1811NucleophileBy similarity
Binding sitei183 – 1831SubstrateBy similarity
Binding sitei214 – 2141FMNBy similarity
Binding sitei242 – 2421FMN; via carbonyl oxygenBy similarity
Binding sitei262 – 2621FMN; via amide nitrogenBy similarity
Binding sitei291 – 2911FMN; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi67 – 715FMNBy similarity
Nucleotide bindingi312 – 3132FMNBy similarity

GO - Molecular functioni

  1. dihydroorotate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
  2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BioCyciHPY:HP1011-MONOMER.
UniPathwayiUPA00070; UER00946.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroorotate dehydrogenase (quinone) (EC:1.3.5.2)
Alternative name(s):
DHOdehase
Short name:
DHOD
Short name:
DHODase
Dihydroorotate oxidase
Gene namesi
Name:pyrD
Ordered Locus Names:HP_1011
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Cell membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Dihydroorotate dehydrogenase (quinone)PRO_0000148445Add
BLAST

Proteomic databases

PRIDEiO25655.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi85962.HP1011.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LX3model-A1-351[»]
ProteinModelPortaliO25655.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni116 – 1205Substrate bindingBy similarity
Regioni243 – 2442Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0167.
KOiK00254.
OMAiNEGHAAA.
OrthoDBiEOG65BDN8.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00225. DHO_dh_type2.
InterProiIPR013785. Aldolase_TIM.
IPR012135. Dihydroorotate_DH_1_2.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamiPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsiTIGR01036. pyrD_sub2. 1 hit.
PROSITEiPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25655-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLYSLVKKYL FSLDAEDAHE KVCKILRMLS SSPFLCNLID SQWGYQNPKL
60 70 80 90 100
ENEILGLHFP NPLGLAAGFD KNASMLRALM AFGFGYLEAG TLTNEAQVGN
110 120 130 140 150
ERPRLFRHIE EESLQNAMGF NNYGAILGVR SFKRFAPYKT PIGINLGKNK
160 170 180 190 200
HIEQAHALED YKAVLSKCLN IGDYYTFNLS SPNTPNLRDL QNKAFVHELF
210 220 230 240 250
CMAKEMTHKP LFLKIAPDLE TDDMLEIVNS AIGAGAHGII ATNTTIDKSL
260 270 280 290 300
VFAPKEMGGL SGKCLTKKSR EIFKELAKAF FNKSVLVSVG GISDAKEAYE
310 320 330 340 350
RIKMGASLLQ IYSAFIYNGP NLCQNILKDL VKLLQKDGFL SVKEAIGADL

R
Length:351
Mass (Da):39,002
Last modified:January 1, 1998 - v1
Checksum:i784D32A23A36F20C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08055.1.
PIRiC64646.
RefSeqiNP_207801.1. NC_000915.1.
YP_006934927.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD08055; AAD08055; HP_1011.
GeneIDi13870204.
899546.
KEGGiheo:C694_05235.
hpy:HP1011.
PATRICi20593357. VBIHelPyl33062_1059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD08055.1 .
PIRi C64646.
RefSeqi NP_207801.1. NC_000915.1.
YP_006934927.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1LX3 model - A 1-351 [» ]
ProteinModelPortali O25655.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 85962.HP1011.

Proteomic databases

PRIDEi O25655.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD08055 ; AAD08055 ; HP_1011 .
GeneIDi 13870204.
899546.
KEGGi heo:C694_05235.
hpy:HP1011.
PATRICi 20593357. VBIHelPyl33062_1059.

Phylogenomic databases

eggNOGi COG0167.
KOi K00254.
OMAi NEGHAAA.
OrthoDBi EOG65BDN8.

Enzyme and pathway databases

UniPathwayi UPA00070 ; UER00946 .
BioCyci HPY:HP1011-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00225. DHO_dh_type2.
InterProi IPR013785. Aldolase_TIM.
IPR012135. Dihydroorotate_DH_1_2.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view ]
Pfami PF01180. DHO_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsi TIGR01036. pyrD_sub2. 1 hit.
PROSITEi PS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiPYRD_HELPY
AccessioniPrimary (citable) accession number: O25655
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3