O25609 (LGT_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prolipoprotein diacylglyceryl transferase
EC=2.4.99.-

Gene names

Name:	lgt
Ordered Locus Names:	HP_0955

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the N-acyl diglyceride group on what will become the N-terminal cysteine of membrane lipoproteins By similarity. HAMAP MF_01147
Pathway	Protein modification; lipoprotein biosynthesis (diacylglyceryl transfer). HAMAP MF_01147
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01147.
Sequence similarities	Belongs to the lgt family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipoprotein biosynthetic process Inferred from electronic annotation. Source: InterPro protein lipoylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Prolipoprotein diacylglyceryl transferase HAMAP MF_01147		PRO_0000172613
Regions
	Transmembrane	14 – 34	21	Helical; Potential
	Transmembrane	62 – 82	21	Helical; Potential
	Transmembrane	106 – 126	21	Helical; Potential
	Transmembrane	136 – 156	21	Helical; Potential
	Transmembrane	190 – 210	21	Helical; Potential
	Transmembrane	218 – 238	21	Helical; Potential
	Transmembrane	252 – 272	21	Helical; Potential
Natural variations
	Natural variant	20	1	S → G in strain: HP439.
	Natural variant	31	1	A → V in strain: HP439.
	Natural variant	76	1	I → V in strain: HP439.
	Natural variant	87	1	G → S in strain: HP439.
	Natural variant	182	1	M → I in strain: HP439.
	Natural variant	202	1	I → V in strain: HP500.
	Natural variant	217	1	H → N in strain: HP500.
	Natural variant	264	1	I → V in strain: HP500.

Sequences

	Sequence		Length	Mass (Da)
	O25609 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 696B3ABAE397FFB5	FASTA	284	32,643
	10 20 30 40 50 60 MNAWNTIYDQ FNPIAFSLGS IEVHWYGLAY ACAIVTAFYM ALRMIQKDPK RFPIERKEFE 70 80 90 100 110 120 SYFLWAELGI VLGARIGYIL IYEPNSGYYL THFWQIFNPF DSHGNFVGIR GMSYHGGLVG 130 140 150 160 170 180 FLIASYLYSR KDLKKLLIYL DLIAISLPLG YVFGRIGNFL NQELVGRIVP KDSHLGQIIG 190 200 210 220 230 240 IMVDNELRYP SQLIEAFLEG VIVFLMVMWA KKHTKTHGLL IVVYGLGYSL MRFIAEFYRE 250 260 270 280 PDSQMGVYFL NLSMGQILSL FMVIVSLGIL LYATKNSKKI KENQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[2]	Goodwin A., Berg D.E., Hoffman P.S. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HP439 and HP500.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07998.1. AF012552 Genomic DNA. Translation: AAC46348.1. AF012553 Genomic DNA. Translation: AAC46350.1.
PIR	C64639.
RefSeq	NP_207747.1. NC_000915.1.
3D structure databases
ProteinModelPortal	O25609.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	899488.
GenomeReviews	Gene locus HP_0955 in contig AE000511_GR.
KEGG	hpy:HP0955.
NMPDR	fig|85962.1.peg.944.
PATRIC	20593223. VBIHelPyl33062_0993.
TIGR	HP_0955.
Phylogenomic databases
HOGENOM	HBG450278.
OMA	NPIAFSL.
ProtClustDB	PRK00052.
Family and domain databases
HAMAP	MF_01147. Lgt. [Tree]
InterPro	IPR001640. Prolipoprot_diAcglycer_Trfase. [Graphical view]
KO	K13292.
Pfam	PF01790. LGT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00544. Lgt. 1 hit.
PROSITE	PS01311. LGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGT_HELPY

Accession

Primary (citable) accession number: O25609
Secondary accession number(s): O30584, O30586

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program