Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 4 Mg2+ or Mn2+ ions per subunit.By similarity

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi283Magnesium or manganese 1UniRule annotation1
Metal bindingi297Magnesium or manganese 1UniRule annotation1
Metal bindingi297Magnesium or manganese 2UniRule annotation1
Metal bindingi299Magnesium or manganese 2UniRule annotation1
Metal bindingi830Magnesium or manganese 3UniRule annotation1
Metal bindingi842Magnesium or manganese 3UniRule annotation1
Metal bindingi842Magnesium or manganese 4UniRule annotation1
Metal bindingi844Magnesium or manganese 4UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi157 – 214ATPUniRule annotationAdd BLAST58
Nucleotide bindingi704 – 764ATPUniRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171
UPA00070; UER00115

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
Gene namesi
Name:carBUniRule annotation
Ordered Locus Names:HP_0919
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001450101 – 1085Carbamoyl-phosphate synthase large chainAdd BLAST1085

Proteomic databases

PaxDbiO25577
PRIDEiO25577

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotation

Protein-protein interaction databases

DIPiDIP-3181N
IntActiO25577, 6 interactors
MINTiO25577
STRINGi85962.HP0919

Structurei

3D structure databases

ProteinModelPortaliO25577
SMRiO25577
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini131 – 326ATP-grasp 1UniRule annotationAdd BLAST196
Domaini678 – 871ATP-grasp 2UniRule annotationAdd BLAST194
Domaini952 – 1085MGS-likePROSITE-ProRule annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 399Carboxyphosphate synthetic domainAdd BLAST399
Regioni400 – 552Oligomerization domainAdd BLAST153
Regioni553 – 951Carbamoyl phosphate synthetic domainAdd BLAST399
Regioni952 – 1085Allosteric domainAdd BLAST134

Sequence similaritiesi

Belongs to the CarB family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105CU6 Bacteria
COG0458 LUCA
KOiK01955
OMAiAVFPFNK

Family and domain databases

CDDicd01424 MGS_CPS_II, 1 hit
Gene3Di1.10.1030.10, 1 hit
3.40.50.1380, 1 hit
HAMAPiMF_01210_B CPSase_L_chain_B, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR006275 CarbamoylP_synth_lsu
IPR005480 CarbamoylP_synth_lsu_oligo
IPR036897 CarbamoylP_synth_lsu_oligo_sf
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR005483 CbamoylP_synth_lsu_CPSase_dom
IPR011607 MGS-like_dom
IPR036914 MGS-like_dom_sf
IPR033937 MGS_CPS_CarB
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF02786 CPSase_L_D2, 2 hits
PF02787 CPSase_L_D3, 1 hit
PF02142 MGS, 1 hit
PRINTSiPR00098 CPSASE
SMARTiView protein in SMART
SM01096 CPSase_L_D3, 1 hit
SM00851 MGS, 1 hit
SUPFAMiSSF48108 SSF48108, 1 hit
SSF52335 SSF52335, 1 hit
SSF52440 SSF52440, 2 hits
TIGRFAMsiTIGR01369 CPSaseII_lrg, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 2 hits
PS00867 CPSASE_2, 2 hits
PS51855 MGS, 1 hit

Sequencei

Sequence statusi: Complete.

O25577-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRTDISNI LLIGSGPIVI GQACEFDYSG TQSCKTLKSL GYRVILINSN
60 70 80 90 100
PATVMTDPEF SHQTYIQPIT PENIATIIEK EKIDAILPTM GGQTALNAVM
110 120 130 140 150
QMHQKGMLEG VELLGAKIEA IKKGEDRQAF KEAMLKIGMD LPKGRYAYTE
160 170 180 190 200
LEALEAINEI GFPAIIRASF TLAGGGSGVA YNIEEFQELA KNALDASPIN
210 220 230 240 250
EILIEESLLG WKEYEMEVIR DSKDNCIIVC CIENIDPMGV HTGDSITIAP
260 270 280 290 300
SLTLTDKEYQ RMRDASFAIL REIGVDTGGS NVQFAIHPET LRMVVIEMNP
310 320 330 340 350
RVSRSSALAS KATGFPIAKV ATMLAVGFSL DEIQNDITNT PASFEPSLDY
360 370 380 390 400
IVVKIPRFAF EKFAGVSSTL GTSMKSIGEV MAIGGNFLEA LQKALCSLEN
410 420 430 440 450
NWLGFESLSK DLEAIKKEIR RPNPKRLLYI ADAFRLGVCV DEVFELCQID
460 470 480 490 500
RWFLSQIQKL VEVEESINSS VLTDAKKLRG LKNLGFSDAR IAAKIKENEN
510 520 530 540 550
LEVSPFEVEL ARSNLQIVPN FEEVDTCAAE FLSLTPYLYS TYAPNPLPPI
560 570 580 590 600
ENKQEKKEKK ILIIGSGPNR IGQGIEFDYC CVHASLALKD LNIKSVMFNC
610 620 630 640 650
NPETVSTDYD TSDTLYFEPI HFECVKSIIQ RERVDGIIVH FGGQTPLKLA
660 670 680 690 700
KDLAKMQAPI IGTPFKVIDI AEDREKFSLF LKELDIKQPK NGMAKSVDEA
710 720 730 740 750
YSIANVIGFP IIVRPSYVLG GQHMQILENI EELRHYLESV THALEISPKN
760 770 780 790 800
PLLIDKFLEK AVELDVDAIC DKKEVYIAGI LQHIEEAGIH SGDSACFIPS
810 820 830 840 850
TLSPEILDEI ERVSAKIALH LGVVGLLNIQ FAVHQNSLYL IEVNPRASRT
860 870 880 890 900
VPFLSKALGV PLAKVATRVM VLEDLKEALK FYDKKNIVGY SKGVYKPKMP
910 920 930 940 950
HFVALKEAVF PFNKLYGSDL ILGPEMKSTG EVMGIARSLG LAFFKAQTAC
960 970 980 990 1000
FNPIKNKGLI FVSIKDKDKE EACVLMKRLV QLGFELCATE GTHKALEKAG
1010 1020 1030 1040 1050
VKSLKVLKIS EGRPNIMDLM MNGEISMAIN TSDHKSQDDA KLIRASVLKN
1060 1070 1080
HVSYFTTLST IEVLLLALEE SSKEDELLAL QDYLK
Length:1,085
Mass (Da):120,074
Last modified:January 1, 1998 - v1
Checksum:i696E124A50DB34C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA Translation: AAD07963.1
PIRiG64634
RefSeqiNP_207711.1, NC_000915.1
WP_001126584.1, NC_018939.1

Genome annotation databases

EnsemblBacteriaiAAD07963; AAD07963; HP_0919
GeneIDi899448
KEGGiheo:C694_04730
hpy:HP0919
PATRICifig|85962.47.peg.984

Similar proteinsi

Entry informationi

Entry nameiCARB_HELPY
AccessioniPrimary (citable) accession number: O25577
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: January 1, 1998
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health