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O25577

- CARB_HELPY

UniProt

O25577 - CARB_HELPY

Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

    Cofactori

    Binds 4 magnesium or manganese ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi283 – 2831Magnesium or manganese 1UniRule annotation
    Metal bindingi297 – 2971Magnesium or manganese 1UniRule annotation
    Metal bindingi297 – 2971Magnesium or manganese 2UniRule annotation
    Metal bindingi299 – 2991Magnesium or manganese 2UniRule annotation
    Metal bindingi830 – 8301Magnesium or manganese 3UniRule annotation
    Metal bindingi842 – 8421Magnesium or manganese 3UniRule annotation
    Metal bindingi842 – 8421Magnesium or manganese 4UniRule annotation
    Metal bindingi844 – 8441Magnesium or manganese 4UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi157 – 21458ATPUniRule annotationAdd
    BLAST
    Nucleotide bindingi704 – 76461ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. manganese ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
    2. arginine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00068; UER00171.
    UPA00070; UER00115.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
    Alternative name(s):
    Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
    Gene namesi
    Name:carBUniRule annotation
    Ordered Locus Names:HP_0919
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10851085Carbamoyl-phosphate synthase large chainPRO_0000145010Add
    BLAST

    Proteomic databases

    PRIDEiO25577.

    Interactioni

    Subunit structurei

    Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotation

    Protein-protein interaction databases

    DIPiDIP-3181N.
    IntActiO25577. 6 interactions.
    MINTiMINT-187278.
    STRINGi85962.HP0919.

    Structurei

    3D structure databases

    ProteinModelPortaliO25577.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini131 – 326196ATP-grasp 1UniRule annotationAdd
    BLAST
    Domaini678 – 871194ATP-grasp 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 399399Carboxyphosphate synthetic domainAdd
    BLAST
    Regioni400 – 552153Oligomerization domainAdd
    BLAST
    Regioni553 – 951399Carbamoyl phosphate synthetic domainAdd
    BLAST
    Regioni952 – 1085134Allosteric domainAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CarB family.UniRule annotation
    Contains 2 ATP-grasp domains.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0458.
    KOiK01955.
    OMAiMDLGTKA.
    OrthoDBiEOG6J1DC6.

    Family and domain databases

    Gene3Di1.10.1030.10. 1 hit.
    3.30.1490.20. 2 hits.
    3.30.470.20. 2 hits.
    3.40.50.1380. 1 hit.
    3.40.50.20. 2 hits.
    HAMAPiMF_01210_B. CPSase_L_chain_B.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006275. CarbamoylP_synth_lsu.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005480. CarbamoylP_synth_lsu_oligo.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR005483. CbamoylP_synth_lsu_CPSase_dom.
    IPR011607. MGS-like_dom.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PfamiPF00289. CPSase_L_chain. 2 hits.
    PF02786. CPSase_L_D2. 2 hits.
    PF02787. CPSase_L_D3. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view]
    PRINTSiPR00098. CPSASE.
    SMARTiSM01096. CPSase_L_D3. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF48108. SSF48108. 1 hit.
    SSF52335. SSF52335. 1 hit.
    SSF52440. SSF52440. 2 hits.
    TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 2 hits.
    PS00867. CPSASE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O25577-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPKRTDISNI LLIGSGPIVI GQACEFDYSG TQSCKTLKSL GYRVILINSN     50
    PATVMTDPEF SHQTYIQPIT PENIATIIEK EKIDAILPTM GGQTALNAVM 100
    QMHQKGMLEG VELLGAKIEA IKKGEDRQAF KEAMLKIGMD LPKGRYAYTE 150
    LEALEAINEI GFPAIIRASF TLAGGGSGVA YNIEEFQELA KNALDASPIN 200
    EILIEESLLG WKEYEMEVIR DSKDNCIIVC CIENIDPMGV HTGDSITIAP 250
    SLTLTDKEYQ RMRDASFAIL REIGVDTGGS NVQFAIHPET LRMVVIEMNP 300
    RVSRSSALAS KATGFPIAKV ATMLAVGFSL DEIQNDITNT PASFEPSLDY 350
    IVVKIPRFAF EKFAGVSSTL GTSMKSIGEV MAIGGNFLEA LQKALCSLEN 400
    NWLGFESLSK DLEAIKKEIR RPNPKRLLYI ADAFRLGVCV DEVFELCQID 450
    RWFLSQIQKL VEVEESINSS VLTDAKKLRG LKNLGFSDAR IAAKIKENEN 500
    LEVSPFEVEL ARSNLQIVPN FEEVDTCAAE FLSLTPYLYS TYAPNPLPPI 550
    ENKQEKKEKK ILIIGSGPNR IGQGIEFDYC CVHASLALKD LNIKSVMFNC 600
    NPETVSTDYD TSDTLYFEPI HFECVKSIIQ RERVDGIIVH FGGQTPLKLA 650
    KDLAKMQAPI IGTPFKVIDI AEDREKFSLF LKELDIKQPK NGMAKSVDEA 700
    YSIANVIGFP IIVRPSYVLG GQHMQILENI EELRHYLESV THALEISPKN 750
    PLLIDKFLEK AVELDVDAIC DKKEVYIAGI LQHIEEAGIH SGDSACFIPS 800
    TLSPEILDEI ERVSAKIALH LGVVGLLNIQ FAVHQNSLYL IEVNPRASRT 850
    VPFLSKALGV PLAKVATRVM VLEDLKEALK FYDKKNIVGY SKGVYKPKMP 900
    HFVALKEAVF PFNKLYGSDL ILGPEMKSTG EVMGIARSLG LAFFKAQTAC 950
    FNPIKNKGLI FVSIKDKDKE EACVLMKRLV QLGFELCATE GTHKALEKAG 1000
    VKSLKVLKIS EGRPNIMDLM MNGEISMAIN TSDHKSQDDA KLIRASVLKN 1050
    HVSYFTTLST IEVLLLALEE SSKEDELLAL QDYLK 1085
    Length:1,085
    Mass (Da):120,074
    Last modified:January 1, 1998 - v1
    Checksum:i696E124A50DB34C1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07963.1.
    PIRiG64634.
    RefSeqiNP_207711.1. NC_000915.1.
    YP_006934834.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD07963; AAD07963; HP_0919.
    GeneIDi13870106.
    899448.
    KEGGiheo:C694_04730.
    hpy:HP0919.
    PATRICi20593139. VBIHelPyl33062_0955.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07963.1 .
    PIRi G64634.
    RefSeqi NP_207711.1. NC_000915.1.
    YP_006934834.1. NC_018939.1.

    3D structure databases

    ProteinModelPortali O25577.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-3181N.
    IntActi O25577. 6 interactions.
    MINTi MINT-187278.
    STRINGi 85962.HP0919.

    Proteomic databases

    PRIDEi O25577.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD07963 ; AAD07963 ; HP_0919 .
    GeneIDi 13870106.
    899448.
    KEGGi heo:C694_04730.
    hpy:HP0919.
    PATRICi 20593139. VBIHelPyl33062_0955.

    Phylogenomic databases

    eggNOGi COG0458.
    KOi K01955.
    OMAi MDLGTKA.
    OrthoDBi EOG6J1DC6.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00171 .
    UPA00070 ; UER00115 .

    Family and domain databases

    Gene3Di 1.10.1030.10. 1 hit.
    3.30.1490.20. 2 hits.
    3.30.470.20. 2 hits.
    3.40.50.1380. 1 hit.
    3.40.50.20. 2 hits.
    HAMAPi MF_01210_B. CPSase_L_chain_B.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006275. CarbamoylP_synth_lsu.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005480. CarbamoylP_synth_lsu_oligo.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR005483. CbamoylP_synth_lsu_CPSase_dom.
    IPR011607. MGS-like_dom.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    Pfami PF00289. CPSase_L_chain. 2 hits.
    PF02786. CPSase_L_D2. 2 hits.
    PF02787. CPSase_L_D3. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00098. CPSASE.
    SMARTi SM01096. CPSase_L_D3. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48108. SSF48108. 1 hit.
    SSF52335. SSF52335. 1 hit.
    SSF52440. SSF52440. 2 hits.
    TIGRFAMsi TIGR01369. CPSaseII_lrg. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 2 hits.
    PS00867. CPSASE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.

    Entry informationi

    Entry nameiCARB_HELPY
    AccessioniPrimary (citable) accession number: O25577
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 2, 2002
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3