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Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 4 Mg(2+) or Mn2+ ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi283 – 2831Magnesium or manganese 1UniRule annotation
Metal bindingi297 – 2971Magnesium or manganese 1UniRule annotation
Metal bindingi297 – 2971Magnesium or manganese 2UniRule annotation
Metal bindingi299 – 2991Magnesium or manganese 2UniRule annotation
Metal bindingi830 – 8301Magnesium or manganese 3UniRule annotation
Metal bindingi842 – 8421Magnesium or manganese 3UniRule annotation
Metal bindingi842 – 8421Magnesium or manganese 4UniRule annotation
Metal bindingi844 – 8441Magnesium or manganese 4UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi157 – 21458ATPUniRule annotationAdd
BLAST
Nucleotide bindingi704 – 76461ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  2. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
Gene namesi
Name:carBUniRule annotation
Ordered Locus Names:HP_0919
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10851085Carbamoyl-phosphate synthase large chainPRO_0000145010Add
BLAST

Proteomic databases

PRIDEiO25577.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotation

Protein-protein interaction databases

DIPiDIP-3181N.
IntActiO25577. 6 interactions.
MINTiMINT-187278.
STRINGi85962.HP0919.

Structurei

3D structure databases

ProteinModelPortaliO25577.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini131 – 326196ATP-grasp 1UniRule annotationAdd
BLAST
Domaini678 – 871194ATP-grasp 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 399399Carboxyphosphate synthetic domainAdd
BLAST
Regioni400 – 552153Oligomerization domainAdd
BLAST
Regioni553 – 951399Carbamoyl phosphate synthetic domainAdd
BLAST
Regioni952 – 1085134Allosteric domainAdd
BLAST

Sequence similaritiesi

Belongs to the CarB family.UniRule annotation
Contains 2 ATP-grasp domains.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0458.
KOiK01955.
OMAiAVFPFNK.
OrthoDBiEOG6J1DC6.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPiMF_01210_B. CPSase_L_chain_B.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25577-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRTDISNI LLIGSGPIVI GQACEFDYSG TQSCKTLKSL GYRVILINSN
60 70 80 90 100
PATVMTDPEF SHQTYIQPIT PENIATIIEK EKIDAILPTM GGQTALNAVM
110 120 130 140 150
QMHQKGMLEG VELLGAKIEA IKKGEDRQAF KEAMLKIGMD LPKGRYAYTE
160 170 180 190 200
LEALEAINEI GFPAIIRASF TLAGGGSGVA YNIEEFQELA KNALDASPIN
210 220 230 240 250
EILIEESLLG WKEYEMEVIR DSKDNCIIVC CIENIDPMGV HTGDSITIAP
260 270 280 290 300
SLTLTDKEYQ RMRDASFAIL REIGVDTGGS NVQFAIHPET LRMVVIEMNP
310 320 330 340 350
RVSRSSALAS KATGFPIAKV ATMLAVGFSL DEIQNDITNT PASFEPSLDY
360 370 380 390 400
IVVKIPRFAF EKFAGVSSTL GTSMKSIGEV MAIGGNFLEA LQKALCSLEN
410 420 430 440 450
NWLGFESLSK DLEAIKKEIR RPNPKRLLYI ADAFRLGVCV DEVFELCQID
460 470 480 490 500
RWFLSQIQKL VEVEESINSS VLTDAKKLRG LKNLGFSDAR IAAKIKENEN
510 520 530 540 550
LEVSPFEVEL ARSNLQIVPN FEEVDTCAAE FLSLTPYLYS TYAPNPLPPI
560 570 580 590 600
ENKQEKKEKK ILIIGSGPNR IGQGIEFDYC CVHASLALKD LNIKSVMFNC
610 620 630 640 650
NPETVSTDYD TSDTLYFEPI HFECVKSIIQ RERVDGIIVH FGGQTPLKLA
660 670 680 690 700
KDLAKMQAPI IGTPFKVIDI AEDREKFSLF LKELDIKQPK NGMAKSVDEA
710 720 730 740 750
YSIANVIGFP IIVRPSYVLG GQHMQILENI EELRHYLESV THALEISPKN
760 770 780 790 800
PLLIDKFLEK AVELDVDAIC DKKEVYIAGI LQHIEEAGIH SGDSACFIPS
810 820 830 840 850
TLSPEILDEI ERVSAKIALH LGVVGLLNIQ FAVHQNSLYL IEVNPRASRT
860 870 880 890 900
VPFLSKALGV PLAKVATRVM VLEDLKEALK FYDKKNIVGY SKGVYKPKMP
910 920 930 940 950
HFVALKEAVF PFNKLYGSDL ILGPEMKSTG EVMGIARSLG LAFFKAQTAC
960 970 980 990 1000
FNPIKNKGLI FVSIKDKDKE EACVLMKRLV QLGFELCATE GTHKALEKAG
1010 1020 1030 1040 1050
VKSLKVLKIS EGRPNIMDLM MNGEISMAIN TSDHKSQDDA KLIRASVLKN
1060 1070 1080
HVSYFTTLST IEVLLLALEE SSKEDELLAL QDYLK
Length:1,085
Mass (Da):120,074
Last modified:January 1, 1998 - v1
Checksum:i696E124A50DB34C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07963.1.
PIRiG64634.
RefSeqiNP_207711.1. NC_000915.1.
YP_006934834.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07963; AAD07963; HP_0919.
GeneIDi13870106.
899448.
KEGGiheo:C694_04730.
hpy:HP0919.
PATRICi20593139. VBIHelPyl33062_0955.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07963.1.
PIRiG64634.
RefSeqiNP_207711.1. NC_000915.1.
YP_006934834.1. NC_018939.1.

3D structure databases

ProteinModelPortaliO25577.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3181N.
IntActiO25577. 6 interactions.
MINTiMINT-187278.
STRINGi85962.HP0919.

Proteomic databases

PRIDEiO25577.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07963; AAD07963; HP_0919.
GeneIDi13870106.
899448.
KEGGiheo:C694_04730.
hpy:HP0919.
PATRICi20593139. VBIHelPyl33062_0955.

Phylogenomic databases

eggNOGiCOG0458.
KOiK01955.
OMAiAVFPFNK.
OrthoDBiEOG6J1DC6.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPiMF_01210_B. CPSase_L_chain_B.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiCARB_HELPY
AccessioniPrimary (citable) accession number: O25577
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.