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O25577

- CARB_HELPY

UniProt

O25577 - CARB_HELPY

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Protein
Carbamoyl-phosphate synthase large chain
Gene
carB, HP_0919
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

Cofactori

Binds 4 magnesium or manganese ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi283 – 2831Magnesium or manganese 1 By similarity
Metal bindingi297 – 2971Magnesium or manganese 1 By similarity
Metal bindingi297 – 2971Magnesium or manganese 2 By similarity
Metal bindingi299 – 2991Magnesium or manganese 2 By similarity
Metal bindingi830 – 8301Magnesium or manganese 3 By similarity
Metal bindingi842 – 8421Magnesium or manganese 3 By similarity
Metal bindingi842 – 8421Magnesium or manganese 4 By similarity
Metal bindingi844 – 8441Magnesium or manganese 4 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi157 – 21458ATP By similarity
Add
BLAST
Nucleotide bindingi704 – 76461ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  2. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chain
Gene namesi
Name:carB
Ordered Locus Names:HP_0919
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10851085Carbamoyl-phosphate synthase large chainUniRule annotation
PRO_0000145010Add
BLAST

Proteomic databases

PRIDEiO25577.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.

Protein-protein interaction databases

DIPiDIP-3181N.
IntActiO25577. 6 interactions.
MINTiMINT-187278.
STRINGi85962.HP0919.

Structurei

3D structure databases

ProteinModelPortaliO25577.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini131 – 326196ATP-grasp 1
Add
BLAST
Domaini678 – 871194ATP-grasp 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 399399Carboxyphosphate synthetic domainUniRule annotation
Add
BLAST
Regioni400 – 552153Oligomerization domainUniRule annotation
Add
BLAST
Regioni553 – 951399Carbamoyl phosphate synthetic domainUniRule annotation
Add
BLAST
Regioni952 – 1085134Allosteric domainUniRule annotation
Add
BLAST

Sequence similaritiesi

Belongs to the CarB family.
Contains 2 ATP-grasp domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0458.
KOiK01955.
OMAiMDLGTKA.
OrthoDBiEOG6J1DC6.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPiMF_01210_B. CPSase_L_chain_B.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25577-1 [UniParc]FASTAAdd to Basket

« Hide

MPKRTDISNI LLIGSGPIVI GQACEFDYSG TQSCKTLKSL GYRVILINSN     50
PATVMTDPEF SHQTYIQPIT PENIATIIEK EKIDAILPTM GGQTALNAVM 100
QMHQKGMLEG VELLGAKIEA IKKGEDRQAF KEAMLKIGMD LPKGRYAYTE 150
LEALEAINEI GFPAIIRASF TLAGGGSGVA YNIEEFQELA KNALDASPIN 200
EILIEESLLG WKEYEMEVIR DSKDNCIIVC CIENIDPMGV HTGDSITIAP 250
SLTLTDKEYQ RMRDASFAIL REIGVDTGGS NVQFAIHPET LRMVVIEMNP 300
RVSRSSALAS KATGFPIAKV ATMLAVGFSL DEIQNDITNT PASFEPSLDY 350
IVVKIPRFAF EKFAGVSSTL GTSMKSIGEV MAIGGNFLEA LQKALCSLEN 400
NWLGFESLSK DLEAIKKEIR RPNPKRLLYI ADAFRLGVCV DEVFELCQID 450
RWFLSQIQKL VEVEESINSS VLTDAKKLRG LKNLGFSDAR IAAKIKENEN 500
LEVSPFEVEL ARSNLQIVPN FEEVDTCAAE FLSLTPYLYS TYAPNPLPPI 550
ENKQEKKEKK ILIIGSGPNR IGQGIEFDYC CVHASLALKD LNIKSVMFNC 600
NPETVSTDYD TSDTLYFEPI HFECVKSIIQ RERVDGIIVH FGGQTPLKLA 650
KDLAKMQAPI IGTPFKVIDI AEDREKFSLF LKELDIKQPK NGMAKSVDEA 700
YSIANVIGFP IIVRPSYVLG GQHMQILENI EELRHYLESV THALEISPKN 750
PLLIDKFLEK AVELDVDAIC DKKEVYIAGI LQHIEEAGIH SGDSACFIPS 800
TLSPEILDEI ERVSAKIALH LGVVGLLNIQ FAVHQNSLYL IEVNPRASRT 850
VPFLSKALGV PLAKVATRVM VLEDLKEALK FYDKKNIVGY SKGVYKPKMP 900
HFVALKEAVF PFNKLYGSDL ILGPEMKSTG EVMGIARSLG LAFFKAQTAC 950
FNPIKNKGLI FVSIKDKDKE EACVLMKRLV QLGFELCATE GTHKALEKAG 1000
VKSLKVLKIS EGRPNIMDLM MNGEISMAIN TSDHKSQDDA KLIRASVLKN 1050
HVSYFTTLST IEVLLLALEE SSKEDELLAL QDYLK 1085
Length:1,085
Mass (Da):120,074
Last modified:January 1, 1998 - v1
Checksum:i696E124A50DB34C1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD07963.1.
PIRiG64634.
RefSeqiNP_207711.1. NC_000915.1.
YP_006934834.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07963; AAD07963; HP_0919.
GeneIDi13870106.
899448.
KEGGiheo:C694_04730.
hpy:HP0919.
PATRICi20593139. VBIHelPyl33062_0955.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD07963.1 .
PIRi G64634.
RefSeqi NP_207711.1. NC_000915.1.
YP_006934834.1. NC_018939.1.

3D structure databases

ProteinModelPortali O25577.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-3181N.
IntActi O25577. 6 interactions.
MINTi MINT-187278.
STRINGi 85962.HP0919.

Proteomic databases

PRIDEi O25577.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD07963 ; AAD07963 ; HP_0919 .
GeneIDi 13870106.
899448.
KEGGi heo:C694_04730.
hpy:HP0919.
PATRICi 20593139. VBIHelPyl33062_0955.

Phylogenomic databases

eggNOGi COG0458.
KOi K01955.
OMAi MDLGTKA.
OrthoDBi EOG6J1DC6.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00171 .
UPA00070 ; UER00115 .

Family and domain databases

Gene3Di 1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPi MF_01210_B. CPSase_L_chain_B.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
Pfami PF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view ]
PRINTSi PR00098. CPSASE.
SMARTi SM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view ]
SUPFAMi SSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsi TIGR01369. CPSaseII_lrg. 1 hit.
PROSITEi PS50975. ATP_GRASP. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiCARB_HELPY
AccessioniPrimary (citable) accession number: O25577
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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