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Protein

Hydrogenase/urease nickel incorporation protein HypB

Gene

hypB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in both the hydrogenase and the urease nickel cofactor insertion step.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Nickel insertion

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciHPY:HP0900-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase/urease nickel incorporation protein HypB
Gene namesi
Name:hypB
Ordered Locus Names:HP_0900
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi59 – 591K → A: No hydrogenase activity, 1% urease activity. Normal amounts of apourease and HypB protein are produced Hydrogenase activity is partially restored and urease activity is fully restored by growth on 5 uM Ni(2+). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 242242Hydrogenase/urease nickel incorporation protein HypBPRO_0000201442Add
BLAST

Proteomic databases

PaxDbiO25560.
PRIDEiO25560.

Interactioni

Protein-protein interaction databases

DIPiDIP-3235N.
IntActiO25560. 2 interactions.
MINTiMINT-163389.
STRINGi85962.HP0900.

Structurei

Secondary structure

1
242
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 4315Combined sources
Beta strandi47 – 526Combined sources
Helixi59 – 668Combined sources
Beta strandi74 – 829Combined sources
Helixi85 – 917Combined sources
Turni92 – 943Combined sources
Beta strandi97 – 1015Combined sources
Helixi110 – 12213Combined sources
Turni123 – 1286Combined sources
Beta strandi130 – 1356Combined sources
Helixi142 – 1454Combined sources
Beta strandi151 – 1588Combined sources
Helixi159 – 1613Combined sources
Helixi165 – 1684Combined sources
Helixi170 – 1745Combined sources
Beta strandi177 – 1826Combined sources
Helixi184 – 1863Combined sources
Turni187 – 1915Combined sources
Helixi194 – 20411Combined sources
Beta strandi210 – 2123Combined sources
Helixi218 – 23417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LPSX-ray2.00A/B1-242[»]
ProteinModelPortaliO25560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105E8T. Bacteria.
COG0378. LUCA.
KOiK04652.
OMAiCPSEFDL.
OrthoDBiEOG628F9T.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003495. CobW/HypB/UreG_dom.
IPR004392. Hyd_acc_HypB.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02492. cobW. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00073. hypB. 1 hit.

Sequencei

Sequence statusi: Complete.

O25560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQRQESLQ NNPNLSKKDV KIVEKILSKN DIKAAEMKER YLKEGLYVLN
60 70 80 90 100
FMSSPGSGKT TMLENLADFK DFKFCVVEGD LQTNRDADRL RKKGVSAHQI
110 120 130 140 150
TTGEACHLEA SMIEGAFDLL KDEGALEKSD FLIIENVGNL VCPSSYNLGA
160 170 180 190 200
AMNIVLLSVP EGDDKVLKYP TMFMCADAVI ISKADMVEVF NFRVSQVKED
210 220 230 240
MQKLKPEAPI FLMSSKDPKS LEDFKNFLLE KKRENYQSTH SF
Length:242
Mass (Da):27,310
Last modified:January 1, 1998 - v1
Checksum:i7C505390FB5C0142
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07946.1.
PIRiD64632.
RefSeqiNP_207693.1. NC_000915.1.
WP_000003622.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07946; AAD07946; HP_0900.
GeneIDi899430.
KEGGiheo:C694_04620.
hpy:HP0900.
PATRICi20593093. VBIHelPyl33062_0932.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07946.1.
PIRiD64632.
RefSeqiNP_207693.1. NC_000915.1.
WP_000003622.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4LPSX-ray2.00A/B1-242[»]
ProteinModelPortaliO25560.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3235N.
IntActiO25560. 2 interactions.
MINTiMINT-163389.
STRINGi85962.HP0900.

Proteomic databases

PaxDbiO25560.
PRIDEiO25560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07946; AAD07946; HP_0900.
GeneIDi899430.
KEGGiheo:C694_04620.
hpy:HP0900.
PATRICi20593093. VBIHelPyl33062_0932.

Phylogenomic databases

eggNOGiENOG4105E8T. Bacteria.
COG0378. LUCA.
KOiK04652.
OMAiCPSEFDL.
OrthoDBiEOG628F9T.

Enzyme and pathway databases

BioCyciHPY:HP0900-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003495. CobW/HypB/UreG_dom.
IPR004392. Hyd_acc_HypB.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02492. cobW. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00073. hypB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.
  2. "Requirement of nickel metabolism proteins HypA and HypB for full activity of both hydrogenase and urease in Helicobacter pylori."
    Olson J.W., Mehta N.S., Maier R.J.
    Mol. Microbiol. 39:176-182(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487.
  3. "Roles of conserved nucleotide-binding domains in accessory proteins, HypB and UreG, in the maturation of nickel-enzymes required for efficient Helicobacter pylori colonization."
    Mehta N., Benoit S., Maier R.J.
    Microb. Pathog. 35:229-234(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-59.
    Strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487.

Entry informationi

Entry nameiHYPB_HELPY
AccessioniPrimary (citable) accession number: O25560
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.