SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O25544

- RUVC_HELPY

UniProt

O25544 - RUVC_HELPY

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Crossover junction endodeoxyribonuclease RuvC
Gene
ruvC, HP_0877
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71Magnesium By similarity
Metal bindingi66 – 661Magnesium By similarity
Metal bindingi139 – 1391Magnesium By similarity
Metal bindingi142 – 1421Magnesium By similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro
  4. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciHPY:HP0877-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:HP_0877
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 157157Crossover junction endodeoxyribonuclease RuvCUniRule annotation
PRO_0000183105Add
BLAST

Proteomic databases

PRIDEiO25544.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HP_0697O254043EBI-9261745,EBI-7513643

Protein-protein interaction databases

IntActiO25544. 4 interactions.
STRINGi85962.HP0877.

Structurei

3D structure databases

ProteinModelPortaliO25544.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.

Phylogenomic databases

eggNOGiCOG0817.
KOiK01159.
OMAiMQIKLAL.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25544-1 [UniParc]FASTAAdd to Basket

« Hide

MRILGIDPGS RKCGYAIISH ASNKLSLITA GFINITTTRL QEQILDLIEA    50
LDCLLDRYEV NEVAIEDIFF GYNPKSVIKL AQFRGALSLK ILERIGNFSE 100
YTPLQVKKAL TGNGKAAKEQ VAFMVKRLLN ITSEIKPLDI SDAIAVAITH 150
AQRLKLH 157
Length:157
Mass (Da):17,424
Last modified:January 1, 1998 - v1
Checksum:i99F99371D309A52C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD07925.1.
PIRiE64629.
RefSeqiNP_207671.1. NC_000915.1.
YP_006934791.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07925; AAD07925; HP_0877.
GeneIDi13870061.
899406.
KEGGiheo:C694_04490.
hpy:HP0877.
PATRICi20593043. VBIHelPyl33062_0909.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD07925.1 .
PIRi E64629.
RefSeqi NP_207671.1. NC_000915.1.
YP_006934791.1. NC_018939.1.

3D structure databases

ProteinModelPortali O25544.
ModBasei Search...

Protein-protein interaction databases

IntActi O25544. 4 interactions.
STRINGi 85962.HP0877.

Proteomic databases

PRIDEi O25544.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD07925 ; AAD07925 ; HP_0877 .
GeneIDi 13870061.
899406.
KEGGi heo:C694_04490.
hpy:HP0877.
PATRICi 20593043. VBIHelPyl33062_0909.

Phylogenomic databases

eggNOGi COG0817.
KOi K01159.
OMAi MQIKLAL.
OrthoDBi EOG6RG044.

Enzyme and pathway databases

BioCyci HPY:HP0877-MONOMER.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00034. RuvC.
InterProi IPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view ]
Pfami PF02075. RuvC. 1 hit.
[Graphical view ]
PRINTSi PR00696. RSOLVASERUVC.
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00228. ruvC. 1 hit.
PROSITEi PS01321. RUVC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiRUVC_HELPY
AccessioniPrimary (citable) accession number: O25544
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi