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O25544 (RUVC_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:HP_0877
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length157 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 157157Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000183105

Sites

Metal binding71Magnesium By similarity
Metal binding661Magnesium By similarity
Metal binding1391Magnesium By similarity
Metal binding1421Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
O25544 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 99F99371D309A52C

FASTA15717,424
        10         20         30         40         50         60 
MRILGIDPGS RKCGYAIISH ASNKLSLITA GFINITTTRL QEQILDLIEA LDCLLDRYEV 

        70         80         90        100        110        120 
NEVAIEDIFF GYNPKSVIKL AQFRGALSLK ILERIGNFSE YTPLQVKKAL TGNGKAAKEQ 

       130        140        150 
VAFMVKRLLN ITSEIKPLDI SDAIAVAITH AQRLKLH 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD07925.1.
PIRE64629.
RefSeqNP_207671.1. NC_000915.1.
YP_006934791.1. NC_018939.1.

3D structure databases

ProteinModelPortalO25544.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING85962.HP0877.

Proteomic databases

PRIDEO25544.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07925; AAD07925; HP_0877.
GeneID13870061.
899406.
KEGGheo:C694_04490.
hpy:HP0877.
PATRIC20593043. VBIHelPyl33062_0909.

Phylogenomic databases

eggNOGCOG0817.
KOK01159.
OMAYTALQMK.
OrthoDBEOG6RG044.
ProtClustDBPRK00039.

Enzyme and pathway databases

BioCycHPY:HP0877-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_HELPY
AccessionPrimary (citable) accession number: O25544
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names