Reviewed, UniProtKB/Swiss-Prot O25525 (GUAC_HELPY)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	HP_0854

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	GMP reductase HAMAP MF_01511		PRO_0000093756
Regions
	Nucleotide binding	205 – 228	24	NADP By similarity
Sites
	Active site	176	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O25525-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: E9ED3053C3E103C8	FASTA	327	36,039
	10 20 30 40 50 60 MSLKVFDYED VQLIPNKCIV NSRSECDTTV ILGKHAFKMP IVPANMQTII NESIAEFLAE 70 80 90 100 110 120 NGYFYIMHRF DGAARIPFVK KMKKRQWISS ISVGVKKEEC LFVEELAKQG LAPDYITIDI 130 140 150 160 170 180 AHGHSNSVIE MIQRIKTHLP ETFVIAGNVG TPEAVRELEN AGADATKVGI GPGKVCITKI 190 200 210 220 230 240 KTGFGTGGWQ LAALRWCAKA ARKPIIADGG IRTHGDIVKS IRFGATMVMI GSLFAGHEES 250 260 270 280 290 300 SGETKIENGI AYKEYFGSAS EFQKGEKKNI EGKKIWIQHK GSLKDTLVEM HQDLQSSISY 310 320 AGGRDLEAIR KVDYVIVKNS IFNGDAI

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD07901.1.
PIR	F64626.
RefSeq	NP_207648.1.
3D structure databases
HSSP	HSSP built from PDB template 1B3O based on UniProtKB P12268.
SMR	O25525. Positions 5-321.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3407N.
Genome annotation databases
GeneID	899383.
GenomeReviews	Gene locus HP_0854 in contig AE000511_GR.
KEGG	hpy:HP0854.
NMPDR	fig|85962.1.peg.845.
TIGR	HP_0854.
Phylogenomic databases
HOGENOM	O25525.
OMA	O25525. NSRSECD.
Enzyme and pathway databases
BRENDA	1.7.1.7. 1131.
Family and domain databases
HAMAP	MF_01511. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_HELPY

Accession

Primary (citable) accession number: O25525

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)