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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37Substrate; via amide nitrogenUniRule annotation1
Binding sitei113ATPUniRule annotation1
Binding sitei158ATPUniRule annotation1
Binding sitei185Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:HP_0845
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001569371 – 259Hydroxyethylthiazole kinaseAdd BLAST259

Proteomic databases

PaxDbiO25516

Interactioni

Protein-protein interaction databases

IntActiO25516, 1 interactor
STRINGi85962.HP0845

Structurei

3D structure databases

ProteinModelPortaliO25516
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U Bacteria
COG2145 LUCA
KOiK00878
OMAiKRPLVHN

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00694 thiM, 1 hit

Sequencei

Sequence statusi: Complete.

O25516-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKELRQKRP LVHNITNYVA AQFVANGLLA LGASPLMSDA IDEMRDLAKI
60 70 80 90 100
SDALAINIGT LNDRAILCAK EAIKHYKALN KPIVLDPVGC SASALRHDTS
110 120 130 140 150
LELLKSGGIS ALRGNAAELG SLVGISCESK GLDSNDAATP VEIIKLAAQK
160 170 180 190 200
YSVIAVMTGK TDYVSDGKKV LSITGGSEYL ALITGAGCLH AAACASFLSL
210 220 230 240 250
KKDPLDSMAQ LCALYKQAAF NAQKKVLENN GSNGSFLFYF LDALSLPIEL

ENSLIKEEW
Length:259
Mass (Da):27,515
Last modified:December 1, 2000 - v2
Checksum:iACC0DCE9B993F730
GO

Sequence cautioni

The sequence AAD07891 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA Translation: AAD07891.1 Different initiation.
PIRiE64625
RefSeqiNP_207638.1, NC_000915.1

Genome annotation databases

EnsemblBacteriaiAAD07891; AAD07891; HP_0845
GeneIDi899374
KEGGiheo:C694_04330
hpy:HP0845
PATRICifig|85962.47.peg.899

Similar proteinsi

Entry informationi

Entry nameiTHIM_HELPY
AccessioniPrimary (citable) accession number: O25516
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 23, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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