O25514 (THIE_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiamine-phosphate synthase
Short name=TP synthase
Short name=TPS
EC=2.5.1.3

Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name=TMP pyrophosphorylase
Short name=TMP-PPase

Gene names

Name:	thiE
Ordered Locus Names:	HP_0843

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) By similarity. HAMAP MF_00097
Catalytic activity	2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate. HAMAP MF_00097
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00097
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1. HAMAP MF_00097
Sequence similarities	Belongs to the thiamine-phosphate synthase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamine-phosphate diphosphorylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Thiamine-phosphate synthase		PRO_0000157017
Regions
	Region	48 – 52	5	HMP-PP binding By similarity
	Region	150 – 152	3	THZ-P binding By similarity
	Region	199 – 200	2	THZ-P binding By similarity
Sites
	Metal binding	85	1	Magnesium By similarity
	Metal binding	104	1	Magnesium By similarity
	Binding site	84	1	HMP-PP By similarity
	Binding site	123	1	HMP-PP By similarity
	Binding site	153	1	HMP-PP By similarity
	Binding site	181	1	THZ-P; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O25514 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 033E0191999238BD	FASTA	219	23,897
	10 20 30 40 50 60 MFDADCLKLM FVAGSQDFYH IKGGKNDRIN ALLDTLELAL QSKITAFQFR QKGDLALQDP 70 80 90 100 110 120 TQIKQLAMKC QKLCQKYGAP FIVNDEVQLA LELKADGVHV GQEDMAIEEV ITLCKKRQFI 130 140 150 160 170 180 GLSVNTLEQA LKARHLDAVA YLGVGPIFPT PSKKDKQVVG VELLKKIKDS GIKKPLIAIG 190 200 210 GITMHNAPKL REYGGIAVIS AIAQAKDKAL AVGKLLNNA

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07889.1.
PIR	C64625.
RefSeq	NP_207636.1. NC_000915.1.
3D structure databases
ProteinModelPortal	O25514.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	899372.
GenomeReviews	Gene locus HP_0843 in contig AE000511_GR.
KEGG	hpy:HP0843.
NMPDR	fig|85962.1.peg.834.
PATRIC	20592979. VBIHelPyl33062_0877.
TIGR	HP_0843.
Phylogenomic databases
HOGENOM	HBG754477.
OMA	FIINDDV.
ProtClustDB	PRK00043.
Family and domain databases
HAMAP	MF_00097. TMP_synthase. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR022998. ThiaminP_synth_SF. IPR003733. TMP_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00788.
Pfam	PF02581. TMP-TENI. 1 hit. [Graphical view]
SUPFAM	SSF51391. TMP_synthase. 1 hit.
TIGRFAMs	TIGR00693. ThiE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIE_HELPY

Accession

Primary (citable) accession number: O25514

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program