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Protein

UDP-N-acetylglucosamine 4,6-dehydratase (inverting)

Gene

pseB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first step in the biosynthesis of pseudaminic acid, a sialic-acid-like sugar that is used to modify flagellin. Has both C6 dehydratase and C5 epimerase activities that result in the production of both UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose and UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine = UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O.1 Publication

Cofactori

NADP+1 Publication

Kineticsi

  1. KM=159 µM for UDP-GlcNAc1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei87NADP; via carbonyl oxygen1 Publication1
    Binding sitei91NADP1 Publication1
    Binding sitei91Substrate1
    Active sitei133Curated1
    Binding sitei141NADP1 Publication1
    Binding sitei145NADP1 Publication1
    Binding sitei173Substrate1
    Binding sitei181Substrate; via amide nitrogen1
    Binding sitei199Substrate1
    Binding sitei258Substrate1
    Binding sitei261Substrate1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi19 – 22NADP1 Publication4
    Nucleotide bindingi43 – 48NADP1 Publication6
    Nucleotide bindingi67 – 68NADP1 Publication2
    Nucleotide bindingi129 – 130NADP1 Publication2
    Nucleotide bindingi174 – 178NADP1 Publication5

    GO - Molecular functioni

    Keywordsi

    Molecular functionLyase
    LigandNADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciHPY:HP0840-MONOMER
    MetaCyc:HP0840-MONOMER
    SABIO-RKiO25511

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylglucosamine 4,6-dehydratase (inverting) (EC:4.2.1.115)
    Alternative name(s):
    Pseudaminic acid biosynthesis protein B
    UDP-GlcNAc-inverting 4,6-dehydratase
    Gene namesi
    Name:pseB
    Synonyms:flaA1
    Ordered Locus Names:HP_0840
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    Proteomesi
    • UP000000429 Componenti: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi133K → A or E: Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004189541 – 333UDP-N-acetylglucosamine 4,6-dehydratase (inverting)Add BLAST333

    Proteomic databases

    PaxDbiO25511
    PRIDEiO25511

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Protein-protein interaction databases

    IntActiO25511, 1 interactor
    STRINGi85962.HP0840

    Structurei

    Secondary structure

    1333
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Turni7 – 10Combined sources4
    Beta strandi12 – 16Combined sources5
    Turni17 – 19Combined sources3
    Helixi21 – 33Combined sources13
    Beta strandi37 – 44Combined sources8
    Helixi46 – 56Combined sources11
    Beta strandi61 – 65Combined sources5
    Helixi71 – 77Combined sources7
    Turni78 – 80Combined sources3
    Beta strandi82 – 86Combined sources5
    Helixi93 – 98Combined sources6
    Helixi100 – 120Combined sources21
    Beta strandi124 – 129Combined sources6
    Helixi132 – 134Combined sources3
    Helixi140 – 154Combined sources15
    Helixi155 – 157Combined sources3
    Beta strandi160 – 162Combined sources3
    Beta strandi165 – 169Combined sources5
    Helixi181 – 191Combined sources11
    Beta strandi196 – 199Combined sources4
    Beta strandi204 – 208Combined sources5
    Helixi210 – 223Combined sources14
    Beta strandi229 – 232Combined sources4
    Beta strandi236 – 238Combined sources3
    Helixi239 – 246Combined sources8
    Beta strandi252 – 254Combined sources3
    Beta strandi266 – 268Combined sources3
    Helixi270 – 275Combined sources6
    Beta strandi276 – 278Combined sources3
    Beta strandi280 – 285Combined sources6
    Beta strandi315 – 319Combined sources5
    Helixi326 – 330Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2GN4X-ray1.90A/B1-333[»]
    2GN6X-ray2.70A/B1-333[»]
    2GN8X-ray2.10A/B1-333[»]
    2GN9X-ray2.80A/B1-333[»]
    2GNAX-ray2.60A/B1-333[»]
    ProteinModelPortaliO25511
    SMRiO25511
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO25511

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the polysaccharide synthase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105C5E Bacteria
    COG1086 LUCA
    KOiK15894
    OMAiMRFFIGD

    Family and domain databases

    CDDicd05237 UDP_invert_4-6DH_SDR_e, 1 hit
    InterProiView protein in InterPro
    IPR036291 NAD(P)-bd_dom_sf
    IPR003869 Polysac_CapD-like
    IPR020025 PseB
    PfamiView protein in Pfam
    PF02719 Polysacc_synt_2, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit
    TIGRFAMsiTIGR03589 PseB, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O25511-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPNHQNMLDN QTILITGGTG SFGKCFVRKV LDTTNAKKII VYSRDELKQS
    60 70 80 90 100
    EMAMEFNDPR MRFFIGDVRD LERLNYALEG VDICIHAAAL KHVPIAEYNP
    110 120 130 140 150
    LECIKTNIMG ASNVINACLK NAISQVIALS TDKAANPINL YGATKLCSDK
    160 170 180 190 200
    LFVSANNFKG SSQTQFSVVR YGNVVGSRGS VVPFFKKLVQ NKASEIPITD
    210 220 230 240 250
    IRMTRFWITL DEGVSFVLKS LKRMHGGEIF VPKIPSMKMT DLAKALAPNT
    260 270 280 290 300
    PTKIIGIRPG EKLHEVMIPK DESHLALEFE DFFIIQPTIS FQTPKDYTLT
    310 320 330
    KLHEKGQKVA PDFEYSSHNN NQWLEPDDLL KLL
    Length:333
    Mass (Da):37,354
    Last modified:January 1, 1998 - v1
    Checksum:i8EF0A39F0FBF0516
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000511 Genomic DNA Translation: AAD07887.1
    PIRiH64624
    RefSeqiNP_207633.1, NC_000915.1
    WP_001132631.1, NC_018939.1

    Genome annotation databases

    EnsemblBacteriaiAAD07887; AAD07887; HP_0840
    GeneIDi899369
    KEGGihpy:HP0840
    PATRICifig|85962.8.peg.874

    Similar proteinsi

    Entry informationi

    Entry nameiPSEB_HELPY
    AccessioniPrimary (citable) accession number: O25511
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: January 1, 1998
    Last modified: March 28, 2018
    This is version 95 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

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