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Protein

Polyamine aminopropyltransferase

Gene

speE

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cell growth and proliferation. Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine. Spermidine cannot be used as an aminopropyl acceptor.1 Publication

Catalytic activityi

S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine.UniRule annotation1 Publication

Enzyme regulationi

Inhibited by methylglyoxal bis(cyclopentylamidinohydrazone)(MGBCP).1 Publication

Pathwayi: spermidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes spermidine from putrescine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Polyamine aminopropyltransferase (speE)
This subpathway is part of the pathway spermidine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes spermidine from putrescine, the pathway spermidine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei6PolyamineUniRule annotation1
Binding sitei29S-adenosylmethioninamineUniRule annotation1
Binding sitei83PolyamineUniRule annotation1
Active sitei155Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • polyamine biosynthetic process Source: UniProtKB
  • spermidine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Polyamine biosynthesis, Spermidine biosynthesis

Enzyme and pathway databases

BioCyciHPY:HP0832-MONOMER.
BRENDAi2.5.1.16. 2604.
UniPathwayiUPA00248; UER00314.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine aminopropyltransferase1 PublicationUniRule annotation
Alternative name(s):
Putrescine aminopropyltransferaseUniRule annotation
Short name:
PAPTUniRule annotation
Spermidine synthase1 PublicationUniRule annotation (EC:2.5.1.16UniRule annotation1 Publication)
Short name:
SPDS1 PublicationUniRule annotation
Short name:
SPDSY1 PublicationUniRule annotation
Gene namesi
Name:speEUniRule annotation
Ordered Locus Names:HP_0832
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564831 – 262Polyamine aminopropyltransferaseAdd BLAST262

Proteomic databases

PaxDbiO25503.

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

STRINGi85962.HP0832.

Structurei

Secondary structure

1262
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Beta strandi11 – 16Combined sources6
Beta strandi18 – 25Combined sources8
Beta strandi30 – 36Combined sources7
Turni37 – 39Combined sources3
Beta strandi40 – 45Combined sources6
Beta strandi48 – 51Combined sources4
Helixi52 – 54Combined sources3
Helixi56 – 67Combined sources12
Beta strandi75 – 81Combined sources7
Helixi84 – 90Combined sources7
Beta strandi96 – 100Combined sources5
Helixi104 – 107Combined sources4
Helixi108 – 110Combined sources3
Turni111 – 113Combined sources3
Helixi117 – 121Combined sources5
Beta strandi126 – 131Combined sources6
Helixi132 – 134Combined sources3
Beta strandi140 – 147Combined sources8
Helixi151 – 158Combined sources8
Beta strandi161 – 172Combined sources12
Turni174 – 176Combined sources3
Helixi178 – 189Combined sources12
Beta strandi193 – 198Combined sources6
Beta strandi208 – 216Combined sources9
Turni219 – 222Combined sources4
Helixi225 – 228Combined sources4
Helixi239 – 244Combined sources6
Helixi250 – 255Combined sources6
Helixi256 – 258Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CMGX-ray2.00A/B1-262[»]
2CMHX-ray2.30A/B/C1-262[»]
ProteinModelPortaliO25503.
SMRiO25503.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO25503.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 249PABSUniRule annotationAdd BLAST249

Sequence similaritiesi

Belongs to the spermidine/spermine synthase family.UniRule annotation
Contains 1 PABS (polyamine biosynthesis) domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG41084QV. Bacteria.
COG0421. LUCA.
KOiK00797.
OMAiISFFPHF.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth. 1 hit.
InterProiIPR030374. PABS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51006. PABS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWITQEITPY LRKEYTIEAK LLDVRSEHNI LEIFKSKDFG EIAMLNRQLL
60 70 80 90 100
FKNFLHIESE LLAHMGGCTK KELKEVLIVD GFDLELAHQL FKYDTHIDFV
110 120 130 140 150
QADEKILDSF ISFFPHFHEV KNNKNFTHAK QLLDLDIKKY DLIFCLQEPD
160 170 180 190 200
IHRIDGLKRM LKEDGVFISV AKHPLLEHVS MQNALKNMGG VFSVAMPFVA
210 220 230 240 250
PLRILSNKGY IYASFKTHPL KDLMTPKIEA LTSVRYYNED IHRAAFALPK
260
NLQEVFKDNI KS
Length:262
Mass (Da):30,539
Last modified:January 1, 1998 - v1
Checksum:iFC368C83B7749C36
GO

Mass spectrometryi

Molecular mass is 31945 Da from positions 1 - 262. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07882.1.
PIRiH64623.
RefSeqiNP_207625.1. NC_000915.1.
WP_000265099.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07882; AAD07882; HP_0832.
GeneIDi899365.
KEGGiheo:C694_04265.
hpy:HP0832.
PATRICi20592955. VBIHelPyl33062_0866.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07882.1.
PIRiH64623.
RefSeqiNP_207625.1. NC_000915.1.
WP_000265099.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CMGX-ray2.00A/B1-262[»]
2CMHX-ray2.30A/B/C1-262[»]
ProteinModelPortaliO25503.
SMRiO25503.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85962.HP0832.

Proteomic databases

PaxDbiO25503.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07882; AAD07882; HP_0832.
GeneIDi899365.
KEGGiheo:C694_04265.
hpy:HP0832.
PATRICi20592955. VBIHelPyl33062_0866.

Phylogenomic databases

eggNOGiENOG41084QV. Bacteria.
COG0421. LUCA.
KOiK00797.
OMAiISFFPHF.

Enzyme and pathway databases

UniPathwayiUPA00248; UER00314.
BioCyciHPY:HP0832-MONOMER.
BRENDAi2.5.1.16. 2604.

Miscellaneous databases

EvolutionaryTraceiO25503.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth. 1 hit.
InterProiIPR030374. PABS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51006. PABS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPEE_HELPY
AccessioniPrimary (citable) accession number: O25503
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.