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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei124 – 1241S-adenosyl-L-methionineUniRule annotation
Binding sitei149 – 1491S-adenosyl-L-methionineUniRule annotation
Binding sitei176 – 1761S-adenosyl-L-methionineUniRule annotation
Binding sitei232 – 2321SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciHPY:HP0747-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:HP_0747
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393tRNA (guanine-N(7)-)-methyltransferasePRO_0000171335Add
BLAST

Proteomic databases

PRIDEiO25443.

Interactioni

Protein-protein interaction databases

DIPiDIP-3501N.
IntActiO25443. 1 interaction.
MINTiMINT-170407.
STRINGi85962.HP0747.

Structurei

3D structure databases

ProteinModelPortaliO25443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiSKYEARW.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25443-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHFLAKLDF KPLEYPLIEG DFCFHREFLS LKNPTKSCVY ASFKDRIFLL
60 70 80 90 100
QKIRRANDFL IKSEKATPLK REVLKQALRI YSQSFEVISH NLQENSKHAS
110 120 130 140 150
GKKTLDLGTF EDFIQKNQAP ILIEIGFGSG RHLIELAKNN PTKTCLGIEI
160 170 180 190 200
HTPSIAQALK QIELLDLKNL HILQGDGRLV LESMPNHRCE KIFVHFPVPW
210 220 230 240 250
NEKKHRRVLS EKFLNEALRV LKPRGFLELR TDDSLYFEDS LKLALKNFQC
260 270 280 290 300
EIEIKKNAQI PVVSKYEARW NKLKKDIYDL RIYSLEWNET PFDNHAFDFS
310 320 330 340 350
FDTITISKKS VGTILKTKKI IQEGYFVHVC NIYENKGDFL VELSMGDFDW
360 370 380 390
PVRLFVLLTE NQIFYLNKSP LKTLNNHKAH LLLQNILSQK GIG
Length:393
Mass (Da):45,774
Last modified:January 1, 1998 - v1
Checksum:iC376EF02D33F71D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07796.1.
PIRiC64613.
RefSeqiNP_207540.1. NC_000915.1.
WP_001119909.1. NC_018939.1.
YP_006934663.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07796; AAD07796; HP_0747.
GeneIDi899331.
KEGGiheo:C694_03845.
hpy:HP0747.
PATRICi20592783. VBIHelPyl33062_0780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07796.1.
PIRiC64613.
RefSeqiNP_207540.1. NC_000915.1.
WP_001119909.1. NC_018939.1.
YP_006934663.1. NC_018939.1.

3D structure databases

ProteinModelPortaliO25443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3501N.
IntActiO25443. 1 interaction.
MINTiMINT-170407.
STRINGi85962.HP0747.

Proteomic databases

PRIDEiO25443.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07796; AAD07796; HP_0747.
GeneIDi899331.
KEGGiheo:C694_03845.
hpy:HP0747.
PATRICi20592783. VBIHelPyl33062_0780.

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiSKYEARW.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciHPY:HP0747-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiTRMB_HELPY
AccessioniPrimary (citable) accession number: O25443
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: January 1, 1998
Last modified: May 27, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.