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O25443 (TRMB_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:HP_0747
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171335

Sites

Binding site1241S-adenosyl-L-methionine By similarity
Binding site1491S-adenosyl-L-methionine By similarity
Binding site1761S-adenosyl-L-methionine By similarity
Binding site2321Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O25443 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: C376EF02D33F71D1

FASTA39345,774
        10         20         30         40         50         60 
MPHFLAKLDF KPLEYPLIEG DFCFHREFLS LKNPTKSCVY ASFKDRIFLL QKIRRANDFL 

        70         80         90        100        110        120 
IKSEKATPLK REVLKQALRI YSQSFEVISH NLQENSKHAS GKKTLDLGTF EDFIQKNQAP 

       130        140        150        160        170        180 
ILIEIGFGSG RHLIELAKNN PTKTCLGIEI HTPSIAQALK QIELLDLKNL HILQGDGRLV 

       190        200        210        220        230        240 
LESMPNHRCE KIFVHFPVPW NEKKHRRVLS EKFLNEALRV LKPRGFLELR TDDSLYFEDS 

       250        260        270        280        290        300 
LKLALKNFQC EIEIKKNAQI PVVSKYEARW NKLKKDIYDL RIYSLEWNET PFDNHAFDFS 

       310        320        330        340        350        360 
FDTITISKKS VGTILKTKKI IQEGYFVHVC NIYENKGDFL VELSMGDFDW PVRLFVLLTE 

       370        380        390 
NQIFYLNKSP LKTLNNHKAH LLLQNILSQK GIG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD07796.1.
PIRC64613.
RefSeqNP_207540.1. NC_000915.1.
YP_006934663.1. NC_018939.1.

3D structure databases

ProteinModelPortalO25443.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-3501N.
IntActO25443. 1 interaction.
MINTMINT-170407.
STRING85962.HP0747.

Proteomic databases

PRIDEO25443.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07796; AAD07796; HP_0747.
GeneID899331.
KEGGheo:C694_03845.
hpy:HP0747.
PATRIC20592783. VBIHelPyl33062_0780.

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMASKYEARW.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycHPY:HP0747-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_HELPY
AccessionPrimary (citable) accession number: O25443
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names