O25443 (TRMB_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	HP_0747

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000171335
Sites
	Binding site	124	1	S-adenosyl-L-methionine By similarity
	Binding site	149	1	S-adenosyl-L-methionine By similarity
	Binding site	176	1	S-adenosyl-L-methionine By similarity
	Binding site	232	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	O25443 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C376EF02D33F71D1	FASTA	393	45,774
	10 20 30 40 50 60 MPHFLAKLDF KPLEYPLIEG DFCFHREFLS LKNPTKSCVY ASFKDRIFLL QKIRRANDFL 70 80 90 100 110 120 IKSEKATPLK REVLKQALRI YSQSFEVISH NLQENSKHAS GKKTLDLGTF EDFIQKNQAP 130 140 150 160 170 180 ILIEIGFGSG RHLIELAKNN PTKTCLGIEI HTPSIAQALK QIELLDLKNL HILQGDGRLV 190 200 210 220 230 240 LESMPNHRCE KIFVHFPVPW NEKKHRRVLS EKFLNEALRV LKPRGFLELR TDDSLYFEDS 250 260 270 280 290 300 LKLALKNFQC EIEIKKNAQI PVVSKYEARW NKLKKDIYDL RIYSLEWNET PFDNHAFDFS 310 320 330 340 350 360 FDTITISKKS VGTILKTKKI IQEGYFVHVC NIYENKGDFL VELSMGDFDW PVRLFVLLTE 370 380 390 NQIFYLNKSP LKTLNNHKAH LLLQNILSQK GIG

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07796.1.
PIR	C64613.
RefSeq	NP_207540.1. NC_000915.1. YP_006934663.1. NC_018939.1.
3D structure databases
ProteinModelPortal	O25443.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3501N.
MINT	MINT-170407.
STRING	85962.HP0747.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07796; AAD07796; HP_0747.
GeneID	13869932. 899331.
KEGG	heo:C694_03845. hpy:HP0747.
PATRIC	20592783. VBIHelPyl33062_0780.
Phylogenomic databases
eggNOG	COG0220.
KO	K03439.
OMA	FVHFPVP.
ProtClustDB	PRK14121.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_HELPY

Accession

Primary (citable) accession number: O25443

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program