O25441 (Y745_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized RNA pseudouridine synthase HP_0745
EC=5.4.99.-

Alternative name(s):
RNA pseudouridylate synthase
RNA-uridine isomerase

Gene names

Ordered Locus Names:

HP_0745

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	RNA uridine = RNA pseudouridine.
Sequence similarities	Belongs to the pseudouridine synthase RluA family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Ligand	RNA-binding
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Uncharacterized RNA pseudouridine synthase HP_0745		PRO_0000162735
Regions
	Domain	12 – 79	68	S4 RNA-binding
Sites
	Active site	136	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O25441 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 40E7F371C9E46117	FASTA	327	37,922
	10 20 30 40 50 60 MQKVFIALTD HKRLDEFLAK ELQISKNQVL NLIKEGLVFC QKKEVKKGGL ALKEGDEITL 70 80 90 100 110 120 LTPKITPKPL KKELDLEIEV IFEDEDLLVL NKPPNLVVHK ALSVKEPTLV DWLEFKNYEL 130 140 150 160 170 180 SNLGLKERYG IVHRLDKDTS GGIVIAKNNF THVHLSEQLK TKMMGRYYIA LLSTPLKEEK 190 200 210 220 230 240 MSVECYLTRN PNNRLKMIAL KAVKKEKSRY SKSEFISLLT SQNNLNLIGA KLFTGRTHQI 250 260 270 280 290 300 RAHLEYLNRH IIGDNLYGLN GALPKEEIRI MLHAYLIEFK HPRSEQKLRF KVPLLKDMLE 310 320 YLKKVFDKEN LDEVLDEEKI LHAFIAK

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07795.1.
PIR	A64613.
RefSeq	NP_207538.1. NC_000915.1. YP_006934661.1. NC_018939.1.
3D structure databases
ProteinModelPortal	O25441.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3289N.
MINT	MINT-165354.
STRING	85962.HP0745.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07795; AAD07795; HP_0745.
GeneID	13869930. 898915.
KEGG	heo:C694_03835. hpy:HP0745.
PATRIC	20592779. VBIHelPyl33062_0778.
Phylogenomic databases
eggNOG	COG0564.
KO	K06180.
OMA	IRIMLHA.
ProtClustDB	CLSK872435.
Family and domain databases
Gene3D	3.10.290.10. 1 hit.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR006225. PsdUridine_synth_RluC/D. IPR006224. PsdUridine_synth_RluC/D_CS. IPR006145. PsdUridine_synth_RsuA/RluD. IPR002942. S4_RNA-bd. [Graphical view]
Pfam	PF00849. PseudoU_synth_2. 1 hit. PF01479. S4. 1 hit. [Graphical view]
SMART	SM00363. S4. 1 hit. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00005. rluA_subfam. 1 hit.
PROSITE	PS01129. PSI_RLU. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y745_HELPY

Accession

Primary (citable) accession number: O25441

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program