O25434 (RIMO_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal protein S12 methylthiotransferase RimO
Short name=S12 MTTase
Short name=S12 methylthiotransferase
EC=2.-.-.-

Alternative name(s):
Ribosome maturation factor RimO

Gene names

Name:	rimO
Ordered Locus Names:	HP_0734

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 By similarity. HAMAP-Rule MF_01865
Cofactor	Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01865.
Sequence similarities	Belongs to the methylthiotransferase family. RimO subfamily. Contains 1 MTTase N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA modification Inferred from electronic annotation. Source: InterPro peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Ribosomal protein S12 methylthiotransferase RimO HAMAP-Rule MF_01865		PRO_0000374856
Regions
	Domain	7 – 119	113	MTTase N-terminal
Sites
	Metal binding	16	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	50	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	82	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	151	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	155	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	158	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O25434 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 59DDFC8721CC42EB	FASTA	439	49,634
	10 20 30 40 50 60 MQVKENKQLC LISLGCSKNL VDSEVMLGKL YNYTLTNDAK SADVILINTC GFIESAKQES 70 80 90 100 110 120 IQTILNAAKD KKEGAILIAS GCLSERYKDE IKELIPEVDI FTGVGDYDKI DIMIAKKQNQ 130 140 150 160 170 180 FSEQVFLSEH YNARIITGSS VHAYVKISEG CNQKCSFCAI PSFKGKLQSR ELDSILKEVE 190 200 210 220 230 240 NLALKGYTDM TFIAQDSSSF LYDKGQKDGL IQLIRAIDKQ QALKSARILY LYPSSTTLEL 250 260 270 280 290 300 IGAIESSPIF QNYFDMPIQH ISDSMLKKMR RNSSQAHHLK LLDAMKQVKE SFIRSTIIVG 310 320 330 340 350 360 HPEENESEFE ELSAFLDEFQ FDRLNIFAFS AEENTHAYSL EKVPKKTINA RIKALNKIAL 370 380 390 400 410 420 KHQNHSFKAL LNKPIKALVE NKEGEYFYKA RDLRWAPEVD GEILINDSEL TTPLKPGHYT 430 IAPSEFKDNI LLAKVLSPF

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07780.1.
PIR	F64611.
RefSeq	NP_207528.1. NC_000915.1. YP_006934650.1. NC_018939.1.
3D structure databases
ProteinModelPortal	O25434.
ModBase	Search...
Protein-protein interaction databases
STRING	85962.HP0734.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07780; AAD07780; HP_0734.
GeneID	13869918. 899326.
KEGG	heo:C694_03770. hpy:HP0734.
PATRIC	20592755. VBIHelPyl33062_0766.
Phylogenomic databases
eggNOG	COG0621.
OMA	RVDTEHM.
ProtClustDB	CLSK872428.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.80.30.20. 1 hit.
HAMAP	MF_01865. MTTase_RimO.
InterPro	IPR006638. Elp3/MiaB/NifB. IPR023970. MeThioTfrase/rSAM. IPR005839. Methylthiotransferase. IPR020612. Methylthiotransferase_CS. IPR013848. Methylthiotransferase_N. IPR012340. NA-bd_OB-fold. IPR005840. Ribosomal_S12_MeSTrfase_RimO. IPR007197. rSAM. IPR023404. rSAM_horseshoe. [Graphical view]
PANTHER	PTHR11918. PTHR11918. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. PF00919. UPF0004. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00089. TIGR00089. 1 hit. TIGR01125. TIGR01125. 1 hit.
PROSITE	PS51449. MTTASE_N. 1 hit. PS01278. MTTASE_RADICAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIMO_HELPY

Accession

Primary (citable) accession number: O25434

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program