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Protein

Ribosomal protein S12 methylthiotransferase RimO

Gene

rimO

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.UniRule annotation

Catalytic activityi

L-aspartate-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthio-L-aspartate-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi151 – 1511Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi155 – 1551Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi158 – 1581Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. transferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid Source: UniProtKB-HAMAP
  2. RNA modification Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciHPY:HP0734-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S12 methylthiotransferase RimOUniRule annotation (EC:2.8.4.4UniRule annotation)
Short name:
S12 MTTaseUniRule annotation
Short name:
S12 methylthiotransferaseUniRule annotation
Alternative name(s):
Ribosomal protein S12 (aspartate-C(3))-methylthiotransferaseUniRule annotation
Ribosome maturation factor RimOUniRule annotation
Gene namesi
Name:rimOUniRule annotation
Ordered Locus Names:HP_0734
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439Ribosomal protein S12 methylthiotransferase RimOPRO_0000374856Add
BLAST

Proteomic databases

PRIDEiO25434.

Interactioni

Protein-protein interaction databases

STRINGi85962.HP0734.

Structurei

3D structure databases

ProteinModelPortaliO25434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 119113MTTase N-terminalUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the methylthiotransferase family. RimO subfamily.UniRule annotation
Contains 1 MTTase N-terminal domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0621.
OMAiCAIPSFK.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.80.30.20. 1 hit.
HAMAPiMF_01865. MTTase_RimO.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR012340. NA-bd_OB-fold.
IPR005840. Ribosomal_S12_MeSTrfase_RimO.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
TIGR01125. TIGR01125. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25434-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQVKENKQLC LISLGCSKNL VDSEVMLGKL YNYTLTNDAK SADVILINTC
60 70 80 90 100
GFIESAKQES IQTILNAAKD KKEGAILIAS GCLSERYKDE IKELIPEVDI
110 120 130 140 150
FTGVGDYDKI DIMIAKKQNQ FSEQVFLSEH YNARIITGSS VHAYVKISEG
160 170 180 190 200
CNQKCSFCAI PSFKGKLQSR ELDSILKEVE NLALKGYTDM TFIAQDSSSF
210 220 230 240 250
LYDKGQKDGL IQLIRAIDKQ QALKSARILY LYPSSTTLEL IGAIESSPIF
260 270 280 290 300
QNYFDMPIQH ISDSMLKKMR RNSSQAHHLK LLDAMKQVKE SFIRSTIIVG
310 320 330 340 350
HPEENESEFE ELSAFLDEFQ FDRLNIFAFS AEENTHAYSL EKVPKKTINA
360 370 380 390 400
RIKALNKIAL KHQNHSFKAL LNKPIKALVE NKEGEYFYKA RDLRWAPEVD
410 420 430
GEILINDSEL TTPLKPGHYT IAPSEFKDNI LLAKVLSPF
Length:439
Mass (Da):49,634
Last modified:January 1, 1998 - v1
Checksum:i59DDFC8721CC42EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07780.1.
PIRiF64611.
RefSeqiNP_207528.1. NC_000915.1.
YP_006934650.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07780; AAD07780; HP_0734.
GeneIDi13869918.
899326.
KEGGiheo:C694_03770.
hpy:HP0734.
PATRICi20592755. VBIHelPyl33062_0766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07780.1.
PIRiF64611.
RefSeqiNP_207528.1. NC_000915.1.
YP_006934650.1. NC_018939.1.

3D structure databases

ProteinModelPortaliO25434.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85962.HP0734.

Proteomic databases

PRIDEiO25434.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07780; AAD07780; HP_0734.
GeneIDi13869918.
899326.
KEGGiheo:C694_03770.
hpy:HP0734.
PATRICi20592755. VBIHelPyl33062_0766.

Phylogenomic databases

eggNOGiCOG0621.
OMAiCAIPSFK.
OrthoDBiEOG6P5ZD8.

Enzyme and pathway databases

BioCyciHPY:HP0734-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.80.30.20. 1 hit.
HAMAPiMF_01865. MTTase_RimO.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR012340. NA-bd_OB-fold.
IPR005840. Ribosomal_S12_MeSTrfase_RimO.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
TIGR01125. TIGR01125. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiRIMO_HELPY
AccessioniPrimary (citable) accession number: O25434
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.