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Protein

Probable tRNA-dihydrouridine synthase

Gene

dus

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.By similarity

Catalytic activityi

5,6-dihydrouracil in tRNA + NAD(P)+ = uracil in tRNA + NAD(P)H.By similarity

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei67FMNBy similarity1
Active sitei98Proton donorBy similarity1
Binding sitei138FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 15FMNBy similarity3
Nucleotide bindingi198 – 200FMNBy similarity3
Nucleotide bindingi222 – 223FMNBy similarity2

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase, RNA-binding, tRNA-binding
Biological processtRNA processing
LigandFlavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciHPY:HP0727-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA-dihydrouridine synthase (EC:1.3.1.-)
Gene namesi
Name:dus
Ordered Locus Names:HP_0727
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001621341 – 328Probable tRNA-dihydrouridine synthaseAdd BLAST328

Proteomic databases

PaxDbiO25427
PRIDEiO25427

Interactioni

Protein-protein interaction databases

DIPiDIP-3615N
IntActiO25427, 1 interactor
MINTiO25427
STRINGi85962.HP0727

Structurei

3D structure databases

ProteinModelPortaliO25427
SMRiO25427
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family.Curated

Phylogenomic databases

eggNOGiENOG4105CEH Bacteria
COG0042 LUCA
KOiK05540
OMAiFRNCVNT

Family and domain databases

CDDicd02801 DUS_like_FMN, 1 hit
Gene3Di1.10.1200.80, 1 hit
3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR035587 DUS-like_FMN-bd
IPR024036 tRNA-dHydroUridine_Synthase_C
IPR004652 tRNA_dU_NifR3
IPR001269 tRNA_hU_synthase
IPR018517 tRNA_hU_synthase_CS
PANTHERiPTHR11082 PTHR11082, 1 hit
PTHR11082:SF25 PTHR11082:SF25, 1 hit
PfamiView protein in Pfam
PF01207 Dus, 1 hit
PIRSFiPIRSF006621 Dus, 1 hit
PROSITEiView protein in PROSITE
PS01136 UPF0034, 1 hit

Sequencei

Sequence statusi: Complete.

O25427-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFKNKKWLF LAPLAGYTDL PFRSVVKKFG VDVTTSEMVS SHSLVYAFDK
60 70 80 90 100
TSKMLEKSPL EDHFMAQISG SKESVVKEAV EKINALEHVN GIDFNCGCPA
110 120 130 140 150
PKVANHGNGS GLLKDLNHLV KLLKTIRENT SKKITSVKVR LGFEKKIPKE
160 170 180 190 200
IAHALNDAPV DYVVVHGRTR SDKYQKDKID YESIALMKKI LKKPVIANGE
210 220 230 240 250
IDSVKKAFEV LQITQADGLM IGRAALRAPW IFWQIRNNTT KLPAVVKKDL
260 270 280 290 300
VLEHFDKMVE FYGDMGVIMF RKNLHAYAKG EMQASAFRNC VNTLTEIKSM
310 320
RESIEEFFNQ EMLQSEVPLW VELNQKSV
Length:328
Mass (Da):37,150
Last modified:January 1, 1998 - v1
Checksum:iD4F3494C7CF0B6B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA Translation: AAD07774.1
PIRiG64610
RefSeqiNP_207521.1, NC_000915.1
WP_000346174.1, NC_018939.1

Genome annotation databases

EnsemblBacteriaiAAD07774; AAD07774; HP_0727
GeneIDi898757
KEGGiheo:C694_03740
hpy:HP0727
PATRICifig|85962.47.peg.776

Similar proteinsi

Entry informationi

Entry nameiDUS_HELPY
AccessioniPrimary (citable) accession number: O25427
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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