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Protein

Probable tRNA-dihydrouridine synthase

Gene

dus

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.By similarity

Catalytic activityi

5,6-dihydrouracil in tRNA + NAD(P)+ = uracil in tRNA + NAD(P)H.By similarity

Cofactori

FMNBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei67FMNBy similarity1
Active sitei98Proton donorBy similarity1
Binding sitei138FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 15FMNBy similarity3
Nucleotide bindingi198 – 200FMNBy similarity3
Nucleotide bindingi222 – 223FMNBy similarity2

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase, RNA-binding, tRNA-binding
Biological processtRNA processing
LigandFlavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciHPY:HP0727-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA-dihydrouridine synthase (EC:1.3.1.-)
Gene namesi
Name:dus
Ordered Locus Names:HP_0727
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001621341 – 328Probable tRNA-dihydrouridine synthaseAdd BLAST328

Proteomic databases

PaxDbiO25427.

Interactioni

Protein-protein interaction databases

DIPiDIP-3615N.
IntActiO25427. 1 interactor.
MINTiMINT-185296.
STRINGi85962.HP0727.

Structurei

3D structure databases

ProteinModelPortaliO25427.
SMRiO25427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family.Curated

Phylogenomic databases

eggNOGiENOG4105CEH. Bacteria.
COG0042. LUCA.
KOiK05540.
OMAiFRNCVNT.

Family and domain databases

CDDicd02801. DUS_like_FMN. 1 hit.
Gene3Di1.10.1200.80. 1 hit.
3.20.20.70. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR035587. DUS-like_FMN-bd.
IPR024036. tRNA-dHydroUridine_Synthase_C.
IPR004652. tRNA_dU_NifR3.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
PANTHERiPTHR11082. PTHR11082. 1 hit.
PTHR11082:SF35. PTHR11082:SF35. 1 hit.
PfamiView protein in Pfam
PF01207. Dus. 1 hit.
PIRSFiPIRSF006621. Dus. 1 hit.
PROSITEiView protein in PROSITE
PS01136. UPF0034. 1 hit.

Sequencei

Sequence statusi: Complete.

O25427-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFKNKKWLF LAPLAGYTDL PFRSVVKKFG VDVTTSEMVS SHSLVYAFDK
60 70 80 90 100
TSKMLEKSPL EDHFMAQISG SKESVVKEAV EKINALEHVN GIDFNCGCPA
110 120 130 140 150
PKVANHGNGS GLLKDLNHLV KLLKTIRENT SKKITSVKVR LGFEKKIPKE
160 170 180 190 200
IAHALNDAPV DYVVVHGRTR SDKYQKDKID YESIALMKKI LKKPVIANGE
210 220 230 240 250
IDSVKKAFEV LQITQADGLM IGRAALRAPW IFWQIRNNTT KLPAVVKKDL
260 270 280 290 300
VLEHFDKMVE FYGDMGVIMF RKNLHAYAKG EMQASAFRNC VNTLTEIKSM
310 320
RESIEEFFNQ EMLQSEVPLW VELNQKSV
Length:328
Mass (Da):37,150
Last modified:January 1, 1998 - v1
Checksum:iD4F3494C7CF0B6B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07774.1.
PIRiG64610.
RefSeqiNP_207521.1. NC_000915.1.
WP_000346174.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07774; AAD07774; HP_0727.
GeneIDi898757.
KEGGiheo:C694_03740.
hpy:HP0727.
PATRICifig|85962.47.peg.776.

Similar proteinsi

Entry informationi

Entry nameiDUS_HELPY
AccessioniPrimary (citable) accession number: O25427
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: January 1, 1998
Last modified: August 30, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families