O25427 (DUS_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA-dihydrouridine synthase
EC=1.-.-.-

Gene names

Name:	dus
Ordered Locus Names:	HP_0727

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs By similarity.
Cofactor	FAD By similarity.
Sequence similarities	Belongs to the dus family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA dihydrouridine synthesis Inferred from electronic annotation. Source: GOC
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro tRNA dihydrouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Probable tRNA-dihydrouridine synthase		PRO_0000162134

Sequences

	Sequence		Length	Mass (Da)
	O25427 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: D4F3494C7CF0B6B3	FASTA	328	37,150
	10 20 30 40 50 60 MDFKNKKWLF LAPLAGYTDL PFRSVVKKFG VDVTTSEMVS SHSLVYAFDK TSKMLEKSPL 70 80 90 100 110 120 EDHFMAQISG SKESVVKEAV EKINALEHVN GIDFNCGCPA PKVANHGNGS GLLKDLNHLV 130 140 150 160 170 180 KLLKTIRENT SKKITSVKVR LGFEKKIPKE IAHALNDAPV DYVVVHGRTR SDKYQKDKID 190 200 210 220 230 240 YESIALMKKI LKKPVIANGE IDSVKKAFEV LQITQADGLM IGRAALRAPW IFWQIRNNTT 250 260 270 280 290 300 KLPAVVKKDL VLEHFDKMVE FYGDMGVIMF RKNLHAYAKG EMQASAFRNC VNTLTEIKSM 310 320 RESIEEFFNQ EMLQSEVPLW VELNQKSV

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07774.1.
PIR	G64610.
RefSeq	NP_207521.1. NC_000915.1. YP_006934644.1. NC_018939.1.
3D structure databases
ProteinModelPortal	O25427.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3615N.
MINT	MINT-185296.
STRING	85962.HP0727.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07774; AAD07774; HP_0727.
GeneID	13869912. 898757.
KEGG	heo:C694_03740. hpy:HP0727.
PATRIC	20592741. VBIHelPyl33062_0759.
Phylogenomic databases
eggNOG	COG0042.
KO	K05540.
OMA	KAPVDYD.
ProtClustDB	CLSK872423.
Family and domain databases
Gene3D	1.10.1200.80. 1 hit. 3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR024036. tRNA-dHydroUridine_Synthase_C. IPR001269. tRNA_hU_synthase. IPR018517. tRNA_hU_synthase_CS. [Graphical view]
PANTHER	PTHR11082. PTHR11082. 1 hit.
Pfam	PF01207. Dus. 1 hit. [Graphical view]
PIRSF	PIRSF006621. Dus. 1 hit.
PROSITE	PS01136. UPF0034. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUS_HELPY

Accession

Primary (citable) accession number: O25427

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program