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Protein

Probable L-asparaginase

Gene

ansA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-asparagine + H2O = L-aspartate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei16 – 161O-isoaspartyl threonine intermediatePROSITE-ProRule annotation
Binding sitei62 – 621SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Probable L-asparaginase (EC:3.5.1.1)
Short name:
L-ASNase
Alternative name(s):
L-asparagine amidohydrolase
Gene namesi
Name:ansA
Synonyms:asn
Ordered Locus Names:HP_0723
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Probable L-asparaginasePRO_0000171081Add
BLAST

Proteomic databases

PaxDbiO25424.
PRIDEiO25424.

Interactioni

Protein-protein interaction databases

DIPiDIP-3115N.
IntActiO25424. 1 interaction.
MINTiMINT-186645.
STRINGi85962.HP0723.

Structurei

3D structure databases

ProteinModelPortaliO25424.
SMRiO25424. Positions 6-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 330325Asparaginase/glutaminasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni95 – 962Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the asparaginase 1 family.Curated
Contains 1 asparaginase/glutaminase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105HDK. Bacteria.
COG0252. LUCA.
KOiK01424.
OMAiDKYDWIV.
OrthoDBiEOG6VTK47.

Family and domain databases

Gene3Di3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
InterProiIPR004550. AsnASE_II.
IPR006034. Asparaginase/glutaminase.
IPR020827. Asparaginase/glutaminase_AS1.
IPR027475. Asparaginase/glutaminase_AS2.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
[Graphical view]
PfamiPF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFiPIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSiPR00139. ASNGLNASE.
SMARTiSM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMiSSF53774. SSF53774. 1 hit.
TIGRFAMsiTIGR00520. asnASE_II. 1 hit.
PROSITEiPS00144. ASN_GLN_ASE_1. 1 hit.
PS00917. ASN_GLN_ASE_2. 1 hit.
PS51732. ASN_GLN_ASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQNLPTIAL LATGGTIAGS GASASLGSYK SGELGIKELL KAIPSLNRLA
60 70 80 90 100
RIQGEQISNI GSQDMNEEVW FKLAKRAQEL LDDSRIQGVV ITHGTDTLEE
110 120 130 140 150
SAYFLNLVLR STKPVVLVGA MRNAASLSAD GALNLYNAVS VALNEKSANK
160 170 180 190 200
GVLVVMDDNI FSAREVIKTH TTHTSTFKAL NSGAIGSVYY GKTRYYMQPL
210 220 230 240 250
RKHTTESEFS LSQLKTPLPK VDIIYTHAGM TPDLFQASLN SHAKGVVIAG
260 270 280 290 300
VGNGNVSAGF LKAMQEASQM GVVIVRSSRV NSGEITSGEI DDKAFITSDN
310 320 330
LNPQKARVLL QLALTKTNNK EKIQEMFEEY
Length:330
Mass (Da):35,539
Last modified:January 1, 1998 - v1
Checksum:i9A2BB6DC7ED9D7DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07772.1.
PIRiC64610.
RefSeqiNP_207517.1. NC_000915.1.
WP_000068360.1. NC_000915.1.

Genome annotation databases

EnsemblBacteriaiAAD07772; AAD07772; HP_0723.
GeneIDi898763.
KEGGiheo:C694_03720.
hpy:HP0723.
PATRICi20592733. VBIHelPyl33062_0755.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07772.1.
PIRiC64610.
RefSeqiNP_207517.1. NC_000915.1.
WP_000068360.1. NC_000915.1.

3D structure databases

ProteinModelPortaliO25424.
SMRiO25424. Positions 6-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3115N.
IntActiO25424. 1 interaction.
MINTiMINT-186645.
STRINGi85962.HP0723.

Proteomic databases

PaxDbiO25424.
PRIDEiO25424.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07772; AAD07772; HP_0723.
GeneIDi898763.
KEGGiheo:C694_03720.
hpy:HP0723.
PATRICi20592733. VBIHelPyl33062_0755.

Phylogenomic databases

eggNOGiENOG4105HDK. Bacteria.
COG0252. LUCA.
KOiK01424.
OMAiDKYDWIV.
OrthoDBiEOG6VTK47.

Family and domain databases

Gene3Di3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
InterProiIPR004550. AsnASE_II.
IPR006034. Asparaginase/glutaminase.
IPR020827. Asparaginase/glutaminase_AS1.
IPR027475. Asparaginase/glutaminase_AS2.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
[Graphical view]
PfamiPF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFiPIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSiPR00139. ASNGLNASE.
SMARTiSM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMiSSF53774. SSF53774. 1 hit.
TIGRFAMsiTIGR00520. asnASE_II. 1 hit.
PROSITEiPS00144. ASN_GLN_ASE_1. 1 hit.
PS00917. ASN_GLN_ASE_2. 1 hit.
PS51732. ASN_GLN_ASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiASPG_HELPY
AccessioniPrimary (citable) accession number: O25424
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.