O25424 (ASPG_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable L-asparaginase
Short name=L-ASNase
EC=3.5.1.1

Alternative name(s):
L-asparagine amidohydrolase

Gene names

Name:	ansA
Synonyms:	asn
Ordered Locus Names:	HP_0723

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the asparaginase 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparagine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Probable L-asparaginase		PRO_0000171081
Regions
	Region	95 – 96	2	Substrate binding By similarity
Sites
	Active site	16	1	O-isoaspartyl threonine intermediate By similarity
	Binding site	62	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O25424 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9A2BB6DC7ED9D7DB	FASTA	330	35,539
	10 20 30 40 50 60 MAQNLPTIAL LATGGTIAGS GASASLGSYK SGELGIKELL KAIPSLNRLA RIQGEQISNI 70 80 90 100 110 120 GSQDMNEEVW FKLAKRAQEL LDDSRIQGVV ITHGTDTLEE SAYFLNLVLR STKPVVLVGA 130 140 150 160 170 180 MRNAASLSAD GALNLYNAVS VALNEKSANK GVLVVMDDNI FSAREVIKTH TTHTSTFKAL 190 200 210 220 230 240 NSGAIGSVYY GKTRYYMQPL RKHTTESEFS LSQLKTPLPK VDIIYTHAGM TPDLFQASLN 250 260 270 280 290 300 SHAKGVVIAG VGNGNVSAGF LKAMQEASQM GVVIVRSSRV NSGEITSGEI DDKAFITSDN 310 320 330 LNPQKARVLL QLALTKTNNK EKIQEMFEEY

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07772.1.
PIR	C64610.
RefSeq	NP_207517.1. NC_000915.1.
3D structure databases
ProteinModelPortal	O25424.
SMR	O25424. Positions 6-330.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3115N.
MINT	MINT-186645.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	898763.
GenomeReviews	Gene locus HP_0723 in contig AE000511_GR.
KEGG	hpy:HP0723.
NMPDR	fig|85962.1.peg.715.
PATRIC	20592733. VBIHelPyl33062_0755.
TIGR	HP_0723.
Phylogenomic databases
HOGENOM	HBG497495.
OMA	LAMVAMT.
ProtClustDB	CLSK496177.
Family and domain databases
InterPro	IPR004550. AsnASE_II. IPR006034. Asparaginase/glutaminase. IPR020827. Asparaginase/glutaminase_CS. [Graphical view]
KO	K01424.
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00710. Asparaginase. 1 hit. [Graphical view]
PIRSF	PIRSF001220. L-ASNase_gatD. 1 hit.
PRINTS	PR00139. ASNGLNASE.
SMART	SM00870. Asparaginase. 1 hit. [Graphical view]
SUPFAM	SSF53774. Asp/Glutamnse. 1 hit.
TIGRFAMs	TIGR00520. AsnASE_II. 1 hit.
PROSITE	PS00144. ASN_GLN_ASE_1. 1 hit. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPG_HELPY

Accession

Primary (citable) accession number: O25424

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program