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O25372 (GATB_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-
Gene names
Name:gatB
Ordered Locus Names:HP_0658
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121

Catalytic activity

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121

ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121

Subunit structure

Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00121

Sequence similarities

Belongs to the GatB/GatE family. GatB subfamily.

Sequence caution

The sequence AAD07723.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

carbon-nitrogen ligase activity, with glutamine as amido-N-donor

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121
PRO_0000148794

Sequences

Sequence LengthMass (Da)Tools
O25372 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 990714A74197B34D

FASTA47453,157
        10         20         30         40         50         60 
MPFEAVIGLE VHVQLNTKTK IFCSCSTSFG ESPNSNTCPV CLGLPGALPV LNKEVVKKAI 

        70         80         90        100        110        120 
QLGTAIEANI NQYSIFARKN YFYPDLPKAY QISQFEVPIV SDGKLEIDTK EGAKIVRIER 

       130        140        150        160        170        180 
AHMEEDAGKN IHEGSYSLVD LNRACTPLLE IVSKPDMRNS EEAIAYLKKL HAIVRFIGIS 

       190        200        210        220        230        240 
DANMQEGNFR CDANVSIRPK GDEKLYTRVE IKNLNSFRFI AKAIEYEIER QSAAWENGRY 

       250        260        270        280        290        300 
NEEVVQETRL FDTHKGITLS MRNKEESADY RYFKDPDLYP VFIDEKLLKE AQKINELPSA 

       310        320        330        340        350        360 
KKIRYMKDFN LKEDDANLLV SDPLLAQYFE SMLHLGVKAK TSVTWLCVEL LGRLKAEITL 

       370        380        390        400        410        420 
ENCGVSTHML GALAKRIDEG KISGKSAKDV LDKLLEEQGG DVDALIEQMG LSQVNDTEAI 

       430        440        450        460        470 
VKVIEEVLKN NADKVLEYKS GKDKLFGFFV GQAMKNLKGA NPSVVNAILK EKLG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD07723.1. Different initiation.
PIRB64602.
RefSeqNP_207452.1. NC_000915.1.
YP_006934583.1. NC_018939.1.

3D structure databases

ProteinModelPortalO25372.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO25372. 1 interaction.
MINTMINT-4059404.
STRING85962.HP0658.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07723; AAD07723; HP_0658.
GeneID13869851.
898991.
KEGGheo:C694_03405.
hpy:HP0658.
PATRIC20592601. VBIHelPyl33062_0689.

Phylogenomic databases

eggNOGCOG0064.
KOK02434.
OMAESADYRY.
OrthoDBEOG6RJV5B.
ProtClustDBPRK05477.

Family and domain databases

HAMAPMF_00121. GatB.
InterProIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERPTHR11659. PTHR11659. 1 hit.
PfamPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMSSF89095. SSF89095. 1 hit.
TIGRFAMsTIGR00133. gatB. 1 hit.
PROSITEPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGATB_HELPY
AccessionPrimary (citable) accession number: O25372
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: February 19, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names