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Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity).By similarity

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (EC:6.3.5.-)
Short name:
Asp/Glu-ADT subunit B
Gene namesi
Name:gatB
Ordered Locus Names:HP_0658
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BPRO_0000148794Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.By similarity

Protein-protein interaction databases

IntActiO25372. 1 interaction.
MINTiMINT-4059404.
STRINGi85962.HP0658.

Structurei

3D structure databases

ProteinModelPortaliO25372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.Curated

Phylogenomic databases

eggNOGiCOG0064.
KOiK02434.
OMAiESADYRY.
OrthoDBiEOG6RJV5B.

Family and domain databases

HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O25372-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPFEAVIGLE VHVQLNTKTK IFCSCSTSFG ESPNSNTCPV CLGLPGALPV
60 70 80 90 100
LNKEVVKKAI QLGTAIEANI NQYSIFARKN YFYPDLPKAY QISQFEVPIV
110 120 130 140 150
SDGKLEIDTK EGAKIVRIER AHMEEDAGKN IHEGSYSLVD LNRACTPLLE
160 170 180 190 200
IVSKPDMRNS EEAIAYLKKL HAIVRFIGIS DANMQEGNFR CDANVSIRPK
210 220 230 240 250
GDEKLYTRVE IKNLNSFRFI AKAIEYEIER QSAAWENGRY NEEVVQETRL
260 270 280 290 300
FDTHKGITLS MRNKEESADY RYFKDPDLYP VFIDEKLLKE AQKINELPSA
310 320 330 340 350
KKIRYMKDFN LKEDDANLLV SDPLLAQYFE SMLHLGVKAK TSVTWLCVEL
360 370 380 390 400
LGRLKAEITL ENCGVSTHML GALAKRIDEG KISGKSAKDV LDKLLEEQGG
410 420 430 440 450
DVDALIEQMG LSQVNDTEAI VKVIEEVLKN NADKVLEYKS GKDKLFGFFV
460 470
GQAMKNLKGA NPSVVNAILK EKLG
Length:474
Mass (Da):53,157
Last modified:May 30, 2000 - v2
Checksum:i990714A74197B34D
GO

Sequence cautioni

The sequence AAD07723.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07723.1. Different initiation.
PIRiB64602.
RefSeqiNP_207452.1. NC_000915.1.
WP_000982564.1. NC_000915.1.
YP_006934583.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07723; AAD07723; HP_0658.
GeneIDi13869851.
898991.
KEGGiheo:C694_03405.
hpy:HP0658.
PATRICi20592601. VBIHelPyl33062_0689.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07723.1. Different initiation.
PIRiB64602.
RefSeqiNP_207452.1. NC_000915.1.
WP_000982564.1. NC_000915.1.
YP_006934583.1. NC_018939.1.

3D structure databases

ProteinModelPortaliO25372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO25372. 1 interaction.
MINTiMINT-4059404.
STRINGi85962.HP0658.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07723; AAD07723; HP_0658.
GeneIDi13869851.
898991.
KEGGiheo:C694_03405.
hpy:HP0658.
PATRICi20592601. VBIHelPyl33062_0689.

Phylogenomic databases

eggNOGiCOG0064.
KOiK02434.
OMAiESADYRY.
OrthoDBiEOG6RJV5B.

Family and domain databases

HAMAPiMF_00121. GatB.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiGATB_HELPY
AccessioniPrimary (citable) accession number: O25372
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: February 4, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.