O25343 (DAPD_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
EC=2.3.1.117

Alternative name(s):
Tetrahydrodipicolinate N-succinyltransferase
Short name=THDP succinyltransferase
Short name=THP succinyltransferase
Tetrahydropicolinate succinylase

Gene names

Name:	dapD
Ordered Locus Names:	HP_0626

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of the cyclic tetrahydrodipicolinate (THDP) into the acyclic N-succinyl-L-2-amino-6-oxopimelate using succinyl-CoA By similarity. HAMAP MF_02122
Catalytic activity	Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H2O = CoA + N-succinyl-L-2-amino-6-oxoheptanedioate. HAMAP MF_02122
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 1/3. HAMAP MF_02122
Subunit structure	Homotrimer By similarity. HAMAP MF_02122
Subcellular location	Cytoplasm By similarity HAMAP MF_02122.
Sequence similarities	Belongs to the type 2 tetrahydrodipicolinate N-succinyltransferase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase HAMAP MF_02122		PRO_0000412260
Regions
	Region	327 – 328	2	Succinyl-CoA binding By similarity
Sites
	Active site	269	1	Acyl-anhydride intermediate By similarity
	Binding site	271	1	Succinyl-CoA By similarity
	Binding site	286	1	Succinyl-CoA; via amide nitrogen By similarity
	Binding site	289	1	Succinyl-CoA By similarity
	Binding site	312	1	Succinyl-CoA; via amide nitrogen By similarity
	Binding site	335	1	Succinyl-CoA; via carbonyl oxygen By similarity
	Binding site	364	1	Succinyl-CoA By similarity

Sequences

	Sequence		Length	Mass (Da)
	O25343 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 122E01CD42414A55	FASTA	401	44,204
	10 20 30 40 50 60 MINKFKNFVS NYQQSNHYKE PLGFGIARVD IAPISKKILC ATYPVLNWKD ENLGSYAVFC 70 80 90 100 110 120 NSLSKEKILK ESASERVIEI DESFVLKALD FYTPFLNEAY SNKMAHKNIQ VVLELLKALE 130 140 150 160 170 180 ENRLKNSDGE SLYRLVILYE DKPCESVESA YMKLLALSLG KAPLRSLNLE GIFNQLSNAA 190 200 210 220 230 240 WSGNKPYELE WLRMNEVALK MRDHFPSIDF IDKFPRYLMQ LIPEFDNIRL LDSSKTRFGA 250 260 270 280 290 300 YLGTGGYTQM PGASYVNFNA GAMGVCMNEG RISSSVVVGA GTDIGGGASV LGVLSGGNNN 310 320 330 340 350 360 PISIGKNCLL GANSVTGISL GDGCIVDAGV AILAGSVIEI EENEFKKLLE VNSALEKHAN 370 380 390 400 NLYKGKELSG KNGVHFRSNS QNGKLIAFRS VKKIELNQNL H

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07696.1.
PIR	B64598.
RefSeq	NP_207420.1. NC_000915.1.
3D structure databases
ProteinModelPortal	O25343.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3232N.
MINT	MINT-183854.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	898801.
GenomeReviews	Gene locus HP_0626 in contig AE000511_GR.
KEGG	hpy:HP0626.
NMPDR	fig|85962.1.peg.618.
PATRIC	20592535. VBIHelPyl33062_0656.
TIGR	HP_0626.
Phylogenomic databases
HOGENOM	HBG303567.
OMA	TGAVECK.
ProtClustDB	CLSK872399.
Family and domain databases
HAMAP	MF_02122. DapD_type2. [Tree]
InterPro	IPR011004. Trimer_LpxA-like. [Graphical view]
KO	K00674.
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DAPD_HELPY

Accession

Primary (citable) accession number: O25343

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 21, 2011
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program