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Protein

DNA polymerase III subunit beta

Gene

dnaN

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: InterPro
  2. DNA binding Source: InterPro
  3. DNA-directed DNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciHPY:HP0500-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit beta (EC:2.7.7.7)
Gene namesi
Name:dnaN
Ordered Locus Names:HP_0500
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429 Componenti: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. DNA polymerase III complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 374374DNA polymerase III subunit betaPRO_0000105438Add
BLAST

Proteomic databases

PRIDEiO25242.

Interactioni

Subunit structurei

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
HP_0418O251723EBI-7532786,EBI-9262675

Protein-protein interaction databases

DIPiDIP-3288N.
IntActiO25242. 2 interactions.
MINTiMINT-176339.
STRINGi85962.HP0500.

Structurei

3D structure databases

ProteinModelPortaliO25242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG0592.
KOiK02338.
OMAiIAFNVRY.
OrthoDBiEOG65J53F.

Family and domain databases

InterProiIPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
[Graphical view]
PfamiPF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
[Graphical view]
SMARTiSM00480. POL3Bc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00663. dnan. 1 hit.

Sequencei

Sequence statusi: Complete.

O25242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKISVSKNDL ENALRYLQAF LDKKDASSIA SHIHLEVIKE KLFLKASDSD
60 70 80 90 100
IGLKSYIFTQ SSDKEGVGTI NGKKFLDIIS CLKDSNIILE TKDDSLAIKQ
110 120 130 140 150
NKSSFKLPMF DADEFPEFPV IDPKVSIEVN APFLVDAFKK IAPVIEQTSH
160 170 180 190 200
KRELAGILMQ FDQKHQTLSV VGTDTKRLSY TQLEKISIHS TEEDISCILP
210 220 230 240 250
KRALLEILKL FYENFSFKSD GMLAVIENEM HTFFTKLIDG NYPDYQKILP
260 270 280 290 300
KEYISSFTLG KEEFKESIKL CSSLSSTIKL TLEKNNALFE SLDSEHSETA
310 320 330 340 350
KTSVEIEKGL DIEKAFHLGV NAKFFLEALN ALGTTQFVLR CNEPSSPFLI
360 370
QESLDEKQSH LNAKISTLMM PITL
Length:374
Mass (Da):42,185
Last modified:December 31, 1997 - v1
Checksum:i5C1FE1C6290FDE6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07565.1.
PIRiD64582.
RefSeqiNP_207297.1. NC_000915.1.
YP_006934422.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07565; AAD07565; HP_0500.
GeneIDi13869690.
899259.
KEGGiheo:C694_02570.
hpy:HP0500.
PATRICi20592275. VBIHelPyl33062_0526.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07565.1.
PIRiD64582.
RefSeqiNP_207297.1. NC_000915.1.
YP_006934422.1. NC_018939.1.

3D structure databases

ProteinModelPortaliO25242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3288N.
IntActiO25242. 2 interactions.
MINTiMINT-176339.
STRINGi85962.HP0500.

Proteomic databases

PRIDEiO25242.

Protocols and materials databases

DNASUi899259.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07565; AAD07565; HP_0500.
GeneIDi13869690.
899259.
KEGGiheo:C694_02570.
hpy:HP0500.
PATRICi20592275. VBIHelPyl33062_0526.

Phylogenomic databases

eggNOGiCOG0592.
KOiK02338.
OMAiIAFNVRY.
OrthoDBiEOG65J53F.

Enzyme and pathway databases

BioCyciHPY:HP0500-MONOMER.

Family and domain databases

InterProiIPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
[Graphical view]
PfamiPF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
[Graphical view]
SMARTiSM00480. POL3Bc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00663. dnan. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiDPO3B_HELPY
AccessioniPrimary (citable) accession number: O25242
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 1998
Last sequence update: December 31, 1997
Last modified: January 6, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.