O25242 (DPO3B_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit beta
EC=2.7.7.7

Gene names

Name:	dnaN
Ordered Locus Names:	HP_0500

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.
Subcellular location	Cytoplasm By similarity.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	DNA polymerase III complex Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	DNA polymerase III subunit beta		PRO_0000105438

Sequences

	Sequence		Length	Mass (Da)
	O25242 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5C1FE1C6290FDE6C	FASTA	374	42,185
	10 20 30 40 50 60 MKISVSKNDL ENALRYLQAF LDKKDASSIA SHIHLEVIKE KLFLKASDSD IGLKSYIFTQ 70 80 90 100 110 120 SSDKEGVGTI NGKKFLDIIS CLKDSNIILE TKDDSLAIKQ NKSSFKLPMF DADEFPEFPV 130 140 150 160 170 180 IDPKVSIEVN APFLVDAFKK IAPVIEQTSH KRELAGILMQ FDQKHQTLSV VGTDTKRLSY 190 200 210 220 230 240 TQLEKISIHS TEEDISCILP KRALLEILKL FYENFSFKSD GMLAVIENEM HTFFTKLIDG 250 260 270 280 290 300 NYPDYQKILP KEYISSFTLG KEEFKESIKL CSSLSSTIKL TLEKNNALFE SLDSEHSETA 310 320 330 340 350 360 KTSVEIEKGL DIEKAFHLGV NAKFFLEALN ALGTTQFVLR CNEPSSPFLI QESLDEKQSH 370 LNAKISTLMM PITL

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07565.1.
PIR	D64582.
RefSeq	NP_207297.1. NC_000915.1. YP_006934422.1. NC_018939.1.
3D structure databases
ProteinModelPortal	O25242.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3288N.
MINT	MINT-176339.
STRING	85962.HP0500.
Protocols and materials databases
DNASU	899259.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07565; AAD07565; HP_0500.
GeneID	13869690. 899259.
KEGG	heo:C694_02570. hpy:HP0500.
PATRIC	20592275. VBIHelPyl33062_0526.
Phylogenomic databases
eggNOG	COG0592.
KO	K02338.
OMA	DYNRVIP.
ProtClustDB	PRK05643.
Family and domain databases
InterPro	IPR001001. DNA_polIII_beta. IPR022635. DNA_polIII_beta_C. IPR022637. DNA_polIII_beta_cen. IPR022634. DNA_polIII_beta_N. [Graphical view]
Pfam	PF00712. DNA_pol3_beta. 1 hit. PF02767. DNA_pol3_beta_2. 1 hit. PF02768. DNA_pol3_beta_3. 1 hit. [Graphical view]
SMART	SM00480. POL3Bc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00663. dnan. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3B_HELPY

Accession

Primary (citable) accession number: O25242

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program