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Protein

DNA polymerase III subunit beta

Gene

dnaN

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciHPY:HP0500-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit beta (EC:2.7.7.7)
Gene namesi
Name:dnaN
Ordered Locus Names:HP_0500
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054381 – 374DNA polymerase III subunit betaAdd BLAST374

Proteomic databases

PaxDbiO25242.

Interactioni

Subunit structurei

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
HP_0418O251723EBI-7532786,EBI-9262675

Protein-protein interaction databases

DIPiDIP-3288N.
IntActiO25242. 2 interactors.
MINTiMINT-176339.
STRINGi85962.HP0500.

Structurei

Secondary structure

1374
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi7 – 17Combined sources11
Helixi18 – 20Combined sources3
Helixi29 – 31Combined sources3
Beta strandi32 – 38Combined sources7
Beta strandi41 – 47Combined sources7
Beta strandi49 – 58Combined sources10
Beta strandi60 – 64Combined sources5
Beta strandi66 – 71Combined sources6
Helixi72 – 80Combined sources9
Beta strandi87 – 92Combined sources6
Beta strandi95 – 100Combined sources6
Beta strandi103 – 108Combined sources6
Helixi112 – 114Combined sources3
Beta strandi124 – 129Combined sources6
Helixi133 – 141Combined sources9
Helixi142 – 144Combined sources3
Helixi153 – 155Combined sources3
Beta strandi156 – 162Combined sources7
Turni163 – 166Combined sources4
Beta strandi167 – 173Combined sources7
Beta strandi175 – 183Combined sources9
Beta strandi188 – 200Combined sources13
Helixi201 – 210Combined sources10
Beta strandi212 – 219Combined sources8
Beta strandi221 – 227Combined sources7
Beta strandi229 – 236Combined sources8
Helixi245 – 247Combined sources3
Beta strandi254 – 260Combined sources7
Helixi261 – 271Combined sources11
Turni272 – 274Combined sources3
Beta strandi276 – 282Combined sources7
Beta strandi284 – 293Combined sources10
Beta strandi301 – 305Combined sources5
Beta strandi316 – 321Combined sources6
Helixi322 – 330Combined sources9
Beta strandi334 – 343Combined sources10
Beta strandi348 – 352Combined sources5
Turni353 – 356Combined sources4
Beta strandi360 – 362Combined sources3
Beta strandi366 – 369Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RKIX-ray2.05A1-374[»]
4S3IX-ray1.95A/B1-374[»]
5FRQX-ray2.90A/B/C/D1-374[»]
ProteinModelPortaliO25242.
SMRiO25242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105CZ8. Bacteria.
COG0592. LUCA.
KOiK02338.
OMAiTHQIRLK.

Family and domain databases

CDDicd00140. beta_clamp. 1 hit.
InterProiIPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
[Graphical view]
PfamiPF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
[Graphical view]
SMARTiSM00480. POL3Bc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00663. dnan. 1 hit.

Sequencei

Sequence statusi: Complete.

O25242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKISVSKNDL ENALRYLQAF LDKKDASSIA SHIHLEVIKE KLFLKASDSD
60 70 80 90 100
IGLKSYIFTQ SSDKEGVGTI NGKKFLDIIS CLKDSNIILE TKDDSLAIKQ
110 120 130 140 150
NKSSFKLPMF DADEFPEFPV IDPKVSIEVN APFLVDAFKK IAPVIEQTSH
160 170 180 190 200
KRELAGILMQ FDQKHQTLSV VGTDTKRLSY TQLEKISIHS TEEDISCILP
210 220 230 240 250
KRALLEILKL FYENFSFKSD GMLAVIENEM HTFFTKLIDG NYPDYQKILP
260 270 280 290 300
KEYISSFTLG KEEFKESIKL CSSLSSTIKL TLEKNNALFE SLDSEHSETA
310 320 330 340 350
KTSVEIEKGL DIEKAFHLGV NAKFFLEALN ALGTTQFVLR CNEPSSPFLI
360 370
QESLDEKQSH LNAKISTLMM PITL
Length:374
Mass (Da):42,185
Last modified:January 1, 1998 - v1
Checksum:i5C1FE1C6290FDE6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07565.1.
PIRiD64582.
RefSeqiNP_207297.1. NC_000915.1.
WP_000704186.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07565; AAD07565; HP_0500.
GeneIDi899259.
KEGGiheo:C694_02570.
hpy:HP0500.
PATRICi20592275. VBIHelPyl33062_0526.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07565.1.
PIRiD64582.
RefSeqiNP_207297.1. NC_000915.1.
WP_000704186.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RKIX-ray2.05A1-374[»]
4S3IX-ray1.95A/B1-374[»]
5FRQX-ray2.90A/B/C/D1-374[»]
ProteinModelPortaliO25242.
SMRiO25242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3288N.
IntActiO25242. 2 interactors.
MINTiMINT-176339.
STRINGi85962.HP0500.

Proteomic databases

PaxDbiO25242.

Protocols and materials databases

DNASUi899259.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07565; AAD07565; HP_0500.
GeneIDi899259.
KEGGiheo:C694_02570.
hpy:HP0500.
PATRICi20592275. VBIHelPyl33062_0526.

Phylogenomic databases

eggNOGiENOG4105CZ8. Bacteria.
COG0592. LUCA.
KOiK02338.
OMAiTHQIRLK.

Enzyme and pathway databases

BioCyciHPY:HP0500-MONOMER.

Family and domain databases

CDDicd00140. beta_clamp. 1 hit.
InterProiIPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
[Graphical view]
PfamiPF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
[Graphical view]
SMARTiSM00480. POL3Bc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00663. dnan. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPO3B_HELPY
AccessioniPrimary (citable) accession number: O25242
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.