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Protein

UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase

Gene

pseC

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the second step in the biosynthesis of pseudaminic acid, a sialic-acid-like sugar that is used to modify flagellin. Uses UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose as substrate producing UDP-4-amino-4,6-dideoxy-beta-L-AltNAc.2 Publications

Catalytic activityi

UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine + 2-oxoglutarate = UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate.2 Publications

Kineticsi

kcat is 43 min(-1) with (2E,6E)-farnesyl UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose as substrate.

  1. KM=0.2 mM for UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei6 – 61Substrate
    Binding sitei56 – 561Substrate; via amide nitrogen
    Binding sitei178 – 1781Substrate
    Binding sitei228 – 2281Substrate

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciHPY:HP0366-MONOMER.
    MetaCyc:MONOMER-14519.
    BRENDAi2.6.1.92. 2604.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase (EC:2.6.1.92)
    Alternative name(s):
    Pseudaminic acid biosynthesis protein C
    Gene namesi
    Name:pseC
    Ordered Locus Names:HP_0366
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    Proteomesi
    • UP000000429 Componenti: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi180 – 1801H → N: Impaired catalytic activity. 1 Publication
    Mutagenesisi183 – 1831K → R: Strongly impaired catalytic activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 375375UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminasePRO_0000418957Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei183 – 1831N6-(pyridoxal phosphate)lysine

    Proteomic databases

    PaxDbiO25130.
    PRIDEiO25130.

    Interactioni

    Protein-protein interaction databases

    STRINGi85962.HP0366.

    Structurei

    Secondary structure

    1
    375
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 63Combined sources
    Helixi13 – 2311Combined sources
    Beta strandi29 – 313Combined sources
    Helixi32 – 4514Combined sources
    Beta strandi48 – 547Combined sources
    Helixi56 – 6611Combined sources
    Beta strandi76 – 794Combined sources
    Beta strandi81 – 833Combined sources
    Helixi86 – 938Combined sources
    Beta strandi97 – 1004Combined sources
    Beta strandi107 – 1093Combined sources
    Helixi111 – 1133Combined sources
    Helixi115 – 1173Combined sources
    Beta strandi122 – 1287Combined sources
    Helixi130 – 1323Combined sources
    Helixi137 – 14711Combined sources
    Beta strandi150 – 1545Combined sources
    Beta strandi170 – 1789Combined sources
    Beta strandi183 – 1853Combined sources
    Beta strandi191 – 1966Combined sources
    Helixi198 – 20710Combined sources
    Beta strandi212 – 2187Combined sources
    Beta strandi220 – 2245Combined sources
    Helixi233 – 24311Combined sources
    Helixi246 – 26419Combined sources
    Beta strandi268 – 2736Combined sources
    Helixi274 – 2763Combined sources
    Beta strandi286 – 2905Combined sources
    Helixi292 – 2976Combined sources
    Helixi298 – 30710Combined sources
    Helixi319 – 3213Combined sources
    Helixi323 – 3297Combined sources
    Helixi335 – 3439Combined sources
    Beta strandi344 – 3474Combined sources
    Helixi355 – 37218Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FN6X-ray2.48A/B1-375[»]
    2FNIX-ray3.00A/B1-375[»]
    2FNUX-ray1.50A/B1-375[»]
    ProteinModelPortaliO25130.
    SMRiO25130. Positions 1-374.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO25130.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni26 – 294Substrate binding
    Regioni313 – 3164Substrate binding

    Sequence similaritiesi

    Belongs to the DegT/DnrJ/EryC1 family.Curated

    Phylogenomic databases

    eggNOGiENOG4105CF4. Bacteria.
    COG0399. LUCA.
    KOiK15895.
    OMAiQKLCKRH.

    Family and domain databases

    CDDicd00616. AHBA_syn. 1 hit.
    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR000653. DegT/StrS_aminotransferase.
    IPR020026. PseC.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF01041. DegT_DnrJ_EryC1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000390. PLP_StrS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR03588. PseC. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O25130-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKEFAYSEPC LDKEDKKAVL EVLNSKQLTQ GKRSLLFEEA LCEFLGVKHA
    60 70 80 90 100
    LVFNSATSAL LTLYRNFSEF SADRNEIITT PISFVATANM LLESGYTPVF
    110 120 130 140 150
    AGIKNDGNID ELALEKLINE RTKAIVSVDY AGKSVEVESV QKLCKKHSLS
    160 170 180 190 200
    FLSDSSHALG SEYQNKKVGG FALASVFSFH AIKPITTAEG GAVVTNDSEL
    210 220 230 240 250
    HEKMKLFRSH GMLKKDFFEG EVKSIGHNFR LNEIQSALGL SQLKKAPFLM
    260 270 280 290 300
    QKREEAALTY DRIFKDNPYF TPLHPLLKDK SSNHLYPILM HQKFFTCKKL
    310 320 330 340 350
    ILESLHKRGI LAQVHYKPIY QYQLYQQLFN TAPLKSAEDF YHAEISLPCH
    360 370
    ANLNLESVQN IAHSVLKTFE SFKIE
    Length:375
    Mass (Da):42,399
    Last modified:January 1, 1998 - v1
    Checksum:iCE6AAD3D475FDF64
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07433.1.
    PIRiF64565.
    RefSeqiNP_207164.1. NC_000915.1.
    WP_000657303.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD07433; AAD07433; HP_0366.
    GeneIDi899219.
    KEGGiheo:C694_01855.
    hpy:HP0366.
    PATRICi20591967. VBIHelPyl33062_0377.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07433.1.
    PIRiF64565.
    RefSeqiNP_207164.1. NC_000915.1.
    WP_000657303.1. NC_018939.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FN6X-ray2.48A/B1-375[»]
    2FNIX-ray3.00A/B1-375[»]
    2FNUX-ray1.50A/B1-375[»]
    ProteinModelPortaliO25130.
    SMRiO25130. Positions 1-374.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi85962.HP0366.

    Proteomic databases

    PaxDbiO25130.
    PRIDEiO25130.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAD07433; AAD07433; HP_0366.
    GeneIDi899219.
    KEGGiheo:C694_01855.
    hpy:HP0366.
    PATRICi20591967. VBIHelPyl33062_0377.

    Phylogenomic databases

    eggNOGiENOG4105CF4. Bacteria.
    COG0399. LUCA.
    KOiK15895.
    OMAiQKLCKRH.

    Enzyme and pathway databases

    BioCyciHPY:HP0366-MONOMER.
    MetaCyc:MONOMER-14519.
    BRENDAi2.6.1.92. 2604.

    Miscellaneous databases

    EvolutionaryTraceiO25130.

    Family and domain databases

    CDDicd00616. AHBA_syn. 1 hit.
    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR000653. DegT/StrS_aminotransferase.
    IPR020026. PseC.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF01041. DegT_DnrJ_EryC1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000390. PLP_StrS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR03588. PseC. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPSEC_HELPY
    AccessioniPrimary (citable) accession number: O25130
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: January 1, 1998
    Last modified: September 7, 2016
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.