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Protein

UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase

Gene

pseC

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the second step in the biosynthesis of pseudaminic acid, a sialic-acid-like sugar that is used to modify flagellin. Uses UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose as substrate producing UDP-4-amino-4,6-dideoxy-beta-L-AltNAc.2 Publications

Catalytic activityi

UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine + 2-oxoglutarate = UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate.2 Publications

Kineticsi

kcat is 43 min(-1) with (2E,6E)-farnesyl UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose as substrate.

  1. KM=0.2 mM for UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei6Substrate1
    Binding sitei56Substrate; via amide nitrogen1
    Binding sitei178Substrate1
    Binding sitei228Substrate1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciHPY:HP0366-MONOMER.
    MetaCyc:MONOMER-14519.
    BRENDAi2.6.1.92. 2604.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase (EC:2.6.1.92)
    Alternative name(s):
    Pseudaminic acid biosynthesis protein C
    Gene namesi
    Name:pseC
    Ordered Locus Names:HP_0366
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    Proteomesi
    • UP000000429 Componenti: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi180H → N: Impaired catalytic activity. 1 Publication1
    Mutagenesisi183K → R: Strongly impaired catalytic activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004189571 – 375UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminaseAdd BLAST375

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei183N6-(pyridoxal phosphate)lysine1

    Proteomic databases

    PaxDbiO25130.

    Interactioni

    Protein-protein interaction databases

    STRINGi85962.HP0366.

    Structurei

    Secondary structure

    1375
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 6Combined sources3
    Helixi13 – 23Combined sources11
    Beta strandi29 – 31Combined sources3
    Helixi32 – 45Combined sources14
    Beta strandi48 – 54Combined sources7
    Helixi56 – 66Combined sources11
    Beta strandi76 – 79Combined sources4
    Beta strandi81 – 83Combined sources3
    Helixi86 – 93Combined sources8
    Beta strandi97 – 100Combined sources4
    Beta strandi107 – 109Combined sources3
    Helixi111 – 113Combined sources3
    Helixi115 – 117Combined sources3
    Beta strandi122 – 128Combined sources7
    Helixi130 – 132Combined sources3
    Helixi137 – 147Combined sources11
    Beta strandi150 – 154Combined sources5
    Beta strandi170 – 178Combined sources9
    Beta strandi183 – 185Combined sources3
    Beta strandi191 – 196Combined sources6
    Helixi198 – 207Combined sources10
    Beta strandi212 – 218Combined sources7
    Beta strandi220 – 224Combined sources5
    Helixi233 – 243Combined sources11
    Helixi246 – 264Combined sources19
    Beta strandi268 – 273Combined sources6
    Helixi274 – 276Combined sources3
    Beta strandi286 – 290Combined sources5
    Helixi292 – 297Combined sources6
    Helixi298 – 307Combined sources10
    Helixi319 – 321Combined sources3
    Helixi323 – 329Combined sources7
    Helixi335 – 343Combined sources9
    Beta strandi344 – 347Combined sources4
    Helixi355 – 372Combined sources18

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2FN6X-ray2.48A/B1-375[»]
    2FNIX-ray3.00A/B1-375[»]
    2FNUX-ray1.50A/B1-375[»]
    ProteinModelPortaliO25130.
    SMRiO25130.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO25130.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni26 – 29Substrate binding4
    Regioni313 – 316Substrate binding4

    Sequence similaritiesi

    Belongs to the DegT/DnrJ/EryC1 family.Curated

    Phylogenomic databases

    eggNOGiENOG4105CF4. Bacteria.
    COG0399. LUCA.
    KOiK15895.
    OMAiQKLCKRH.

    Family and domain databases

    CDDicd00616. AHBA_syn. 1 hit.
    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR000653. DegT/StrS_aminotransferase.
    IPR020026. PseC.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF01041. DegT_DnrJ_EryC1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000390. PLP_StrS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR03588. PseC. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O25130-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKEFAYSEPC LDKEDKKAVL EVLNSKQLTQ GKRSLLFEEA LCEFLGVKHA
    60 70 80 90 100
    LVFNSATSAL LTLYRNFSEF SADRNEIITT PISFVATANM LLESGYTPVF
    110 120 130 140 150
    AGIKNDGNID ELALEKLINE RTKAIVSVDY AGKSVEVESV QKLCKKHSLS
    160 170 180 190 200
    FLSDSSHALG SEYQNKKVGG FALASVFSFH AIKPITTAEG GAVVTNDSEL
    210 220 230 240 250
    HEKMKLFRSH GMLKKDFFEG EVKSIGHNFR LNEIQSALGL SQLKKAPFLM
    260 270 280 290 300
    QKREEAALTY DRIFKDNPYF TPLHPLLKDK SSNHLYPILM HQKFFTCKKL
    310 320 330 340 350
    ILESLHKRGI LAQVHYKPIY QYQLYQQLFN TAPLKSAEDF YHAEISLPCH
    360 370
    ANLNLESVQN IAHSVLKTFE SFKIE
    Length:375
    Mass (Da):42,399
    Last modified:January 1, 1998 - v1
    Checksum:iCE6AAD3D475FDF64
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07433.1.
    PIRiF64565.
    RefSeqiNP_207164.1. NC_000915.1.
    WP_000657303.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD07433; AAD07433; HP_0366.
    GeneIDi899219.
    KEGGiheo:C694_01855.
    hpy:HP0366.
    PATRICi20591967. VBIHelPyl33062_0377.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07433.1.
    PIRiF64565.
    RefSeqiNP_207164.1. NC_000915.1.
    WP_000657303.1. NC_018939.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2FN6X-ray2.48A/B1-375[»]
    2FNIX-ray3.00A/B1-375[»]
    2FNUX-ray1.50A/B1-375[»]
    ProteinModelPortaliO25130.
    SMRiO25130.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi85962.HP0366.

    Proteomic databases

    PaxDbiO25130.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAD07433; AAD07433; HP_0366.
    GeneIDi899219.
    KEGGiheo:C694_01855.
    hpy:HP0366.
    PATRICi20591967. VBIHelPyl33062_0377.

    Phylogenomic databases

    eggNOGiENOG4105CF4. Bacteria.
    COG0399. LUCA.
    KOiK15895.
    OMAiQKLCKRH.

    Enzyme and pathway databases

    BioCyciHPY:HP0366-MONOMER.
    MetaCyc:MONOMER-14519.
    BRENDAi2.6.1.92. 2604.

    Miscellaneous databases

    EvolutionaryTraceiO25130.

    Family and domain databases

    CDDicd00616. AHBA_syn. 1 hit.
    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR000653. DegT/StrS_aminotransferase.
    IPR020026. PseC.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF01041. DegT_DnrJ_EryC1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000390. PLP_StrS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR03588. PseC. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPSEC_HELPY
    AccessioniPrimary (citable) accession number: O25130
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: January 1, 1998
    Last modified: November 2, 2016
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.