O25103 (HCPB_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase HcpB
EC=3.5.2.6

Alternative name(s):
Cysteine-rich protein B

Gene names

Name:	hcpB
Ordered Locus Names:	HP_0336

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes 6-aminopenicillinic acid and 7-aminocephalosporanic acid (ACA) derivatives. Ref.3
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Miscellaneous	Antibodies against HcpB are present in sera from human patients infected by Helicobacter pylori.
Sequence similarities	Belongs to the hcp beta-lactamase family. Contains 3 TPR repeats.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Repeat TPR repeat
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 138

138

Beta-lactamase HcpB

PRO_0000221379

Regions

Repeat

1 – 28

28

TPR 1

Repeat

57 – 94

38

TPR 2

Repeat

97 – 130

34

TPR 3

Amino acid modifications

Disulfide bond

22 ↔ 30

Disulfide bond

52 ↔ 60

Disulfide bond

88 ↔ 96

Disulfide bond

124 ↔ 132

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

138

Helix Strand Turn

Details...

Helix

7 – 23

17

Helix

29 – 34

6

Helix

41 – 53

13

Helix

57 – 69

13

Beta strand

71 – 73

3

Helix

77 – 89

13

Helix

93 – 105

13

Beta strand

107 – 109

3

Helix

113 – 125

13

Helix

129 – 134

6

Sequences

	Sequence		Length	Mass (Da)
	O25103 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 4149505C1BE623B1	FASTA	138	15,346
	10 20 30 40 50 60 MVGGGTVKKD LKKAIQYYVK ACELNEMFGC LSLVSNSQIN KQKLFQYLSK ACELNSGNGC 70 80 90 100 110 120 RFLGDFYENG KYVKKDLRKA AQYYSKACGL NDQDGCLILG YKQYAGKGVV KNEKQAVKTF 130 EKACRLGSED ACGILNNY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[2]	"Detection of high titers of antibody against Helicobacter cysteine-rich proteins A, B, C, and E in Helicobacter pylori-infected individuals." Mittl P.R.E., Luethy L., Reinhardt C., Joller H. Clin. Diagn. Lab. Immunol. 10:542-545(2003) [PubMed] [Europe PMC] [Abstract] Cited for: ANTIGENICITY.
[3]	"The crystal structure of Helicobacter pylori cysteine-rich protein B reveals a novel fold for a penicillin-binding protein." Luethy L., Gruetter M.G., Mittl P.R.E. J. Biol. Chem. 277:10187-10193(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS), FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000511 Genomic DNA. Translation: AAD07408.1.

PIR

H64561.

RefSeq

NP_207134.1. NC_000915.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KLX	X-ray	1.95	A	1-138	[»]

ProteinModelPortal

O25103.

SMR

O25103. Positions 3-135.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-3342N.

MINT

MINT-160080.

STRING

85962.HP0336.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAD07408; AAD07408; HP_0336.

GeneID

899210.

KEGG

hpy:HP0336.

PATRIC

20591907. VBIHelPyl33062_0350.

Phylogenomic databases

eggNOG

COG0790.

KO

K07126.

OMA

SIACEAN.

Family and domain databases

Gene3D

1.25.40.10. 1 hit.

InterPro

IPR006597. Sel1-like.
IPR011990. TPR-like_helical.
[Graphical view]

Pfam

PF08238. Sel1. 3 hits.
[Graphical view]

SMART

SM00671. SEL1. 3 hits.
[Graphical view]

PROSITE

PS50005. TPR. False negative.
PS50293. TPR_REGION. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O25103.

Entry information

Entry name

HCPB_HELPY

Accession

Primary (citable) accession number: O25103

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program