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O25103 (HCPB_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase HcpB

EC=3.5.2.6
Alternative name(s):
Cysteine-rich protein B
Gene names
Name:hcpB
Ordered Locus Names:HP_0336
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length138 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes 6-aminopenicillinic acid and 7-aminocephalosporanic acid (ACA) derivatives. Ref.3

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Miscellaneous

Antibodies against HcpB are present in sera from human patients infected by Helicobacter pylori.

Sequence similarities

Belongs to the hcp beta-lactamase family.

Contains 3 TPR repeats.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainRepeat
TPR repeat
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmetabolic process

Inferred from direct assay Ref.3. Source: GOC

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from direct assay Ref.3. Source: CACAO

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 138138Beta-lactamase HcpB
PRO_0000221379

Regions

Repeat1 – 2828TPR 1
Repeat57 – 9438TPR 2
Repeat97 – 13034TPR 3

Amino acid modifications

Disulfide bond22 ↔ 30
Disulfide bond52 ↔ 60
Disulfide bond88 ↔ 96
Disulfide bond124 ↔ 132

Secondary structure

..................... 138
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O25103 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 4149505C1BE623B1

FASTA13815,346
        10         20         30         40         50         60 
MVGGGTVKKD LKKAIQYYVK ACELNEMFGC LSLVSNSQIN KQKLFQYLSK ACELNSGNGC 

        70         80         90        100        110        120 
RFLGDFYENG KYVKKDLRKA AQYYSKACGL NDQDGCLILG YKQYAGKGVV KNEKQAVKTF 

       130 
EKACRLGSED ACGILNNY 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of the gastric pathogen Helicobacter pylori."
Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. expand/collapse author list , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.
Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700392 / 26695.
[2]"Detection of high titers of antibody against Helicobacter cysteine-rich proteins A, B, C, and E in Helicobacter pylori-infected individuals."
Mittl P.R.E., Luethy L., Reinhardt C., Joller H.
Clin. Diagn. Lab. Immunol. 10:542-545(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ANTIGENICITY.
[3]"The crystal structure of Helicobacter pylori cysteine-rich protein B reveals a novel fold for a penicillin-binding protein."
Luethy L., Gruetter M.G., Mittl P.R.E.
J. Biol. Chem. 277:10187-10193(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS), FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD07408.1.
PIRH64561.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KLXX-ray1.95A1-138[»]
ProteinModelPortalO25103.
SMRO25103. Positions 3-135.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-3342N.
IntActO25103. 50 interactions.
MINTMINT-160080.
STRING85962.HP0336.

Proteomic databases

PRIDEO25103.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07408; AAD07408; HP_0336.
PATRIC20591907. VBIHelPyl33062_0350.

Phylogenomic databases

eggNOGCOG0790.
OMASIACEAN.
OrthoDBEOG6TJ7WZ.

Enzyme and pathway databases

BioCycHPY:HP0336-MONOMER.

Family and domain databases

Gene3D1.25.40.10. 1 hit.
InterProIPR006597. Sel1-like.
IPR011990. TPR-like_helical.
[Graphical view]
PfamPF08238. Sel1. 4 hits.
[Graphical view]
SMARTSM00671. SEL1. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO25103.

Entry information

Entry nameHCPB_HELPY
AccessionPrimary (citable) accession number: O25103
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names