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Protein

Ketol-acid reductoisomerase (NADP(+))

Gene

ilvC

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.UniRule annotation

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.UniRule annotation
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. no protein annotated in this organism
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. no protein annotated in this organism
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52NADPUniRule annotation1
Binding sitei54NADPUniRule annotation1
Active sitei109UniRule annotation1
Binding sitei135NADP; via amide nitrogenUniRule annotation1
Metal bindingi193Magnesium 1UniRule annotation1
Metal bindingi193Magnesium 2UniRule annotation1
Metal bindingi197Magnesium 1UniRule annotation1
Metal bindingi229Magnesium 2UniRule annotation1
Metal bindingi233Magnesium 2UniRule annotation1
Binding sitei254SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 29NADPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandMagnesium, Metal-binding, NADP

Enzyme and pathway databases

BioCyciHPY:HP0330-MONOMER.
UniPathwayiUPA00047; UER00056.
UPA00049; UER00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase (NADP(+))UniRule annotation (EC:1.1.1.86UniRule annotation)
Short name:
KARIUniRule annotation
Alternative name(s):
Acetohydroxy-acid isomeroreductaseUniRule annotation
Short name:
AHIRUniRule annotation
Alpha-keto-beta-hydroxylacyl reductoisomeraseUniRule annotation
Ketol-acid reductoisomerase type 1UniRule annotation
Ketol-acid reductoisomerase type IUniRule annotation
Gene namesi
Name:ilvCUniRule annotation
Ordered Locus Names:HP_0330
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001513161 – 330Ketol-acid reductoisomerase (NADP(+))Add BLAST330

Proteomic databases

PaxDbiO25097.

Interactioni

Protein-protein interaction databases

STRINGi85962.HP0330.

Structurei

3D structure databases

ProteinModelPortaliO25097.
SMRiO25097.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 184KARI N-terminal RossmannPROSITE-ProRule annotationAdd BLAST182
Domaini185 – 329KARI C-terminal knottedPROSITE-ProRule annotationAdd BLAST145

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6M. Bacteria.
COG0059. LUCA.
KOiK00053.
OMAiRAMFSWL.

Family and domain databases

HAMAPiMF_00435. IlvC. 1 hit.
InterProiView protein in InterPro
IPR008927. 6-PGluconate_DH-like_C_sf.
IPR000506. AcH_isomrdctse_C.
IPR013116. IlvN.
IPR013023. KARI.
IPR014359. KARI_prok.
IPR036291. NAD(P)-bd_dom_sf.
PANTHERiPTHR21371. PTHR21371. 1 hit.
PfamiView protein in Pfam
PF01450. IlvC. 1 hit.
PF07991. IlvN. 1 hit.
PIRSFiPIRSF000116. IlvC_gammaproteo. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00465. ilvC. 1 hit.
PROSITEiView protein in PROSITE
PS51851. KARI_C. 1 hit.
PS51850. KARI_N. 1 hit.

Sequencei

Sequence statusi: Complete.

O25097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPVYYDKD IDLGVIQSLQ VGIIGYGAQG EAQALNLRDS KVKARIGLYQ
60 70 80 90 100
GSLSVSKAKK EGFEVLEVKE LVQNSDVIMA LLPDELHKEV LEKEVIPFLK
110 120 130 140 150
EGQIVGFAHG FSVHFNQVVL PKGVGAILVA PKGPGSALRE EYLKNRGLYH
160 170 180 190 200
LIAIEQESSI HNAKAVALSY AKAMGGGRMG VLETSFKEEC ESDLFGEQAV
210 220 230 240 250
LCGGLEAIVR MGFETLIKAG YPEELAYFEC VHEVKLVADL LHYKGVEGLR
260 270 280 290 300
KHISNTAEFG AIKAREPMGK LLKKRMQKIL KKIQNGSFAK DFLLEKSLNY
310 320 330
PRLNTERKAL KETKIEQIGE ILRAPFNHKK
Length:330
Mass (Da):36,534
Last modified:January 1, 1998 - v1
Checksum:i97DAE57B06C3FC38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07399.1.
PIRiB64561.
RefSeqiNP_207128.1. NC_000915.1.
WP_001207734.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07399; AAD07399; HP_0330.
GeneIDi899086.
KEGGiheo:C694_01670.
hpy:HP0330.
PATRICifig|85962.47.peg.352.

Similar proteinsi

Entry informationi

Entry nameiILVC_HELPY
AccessioniPrimary (citable) accession number: O25097
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: February 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome