O25096 (NADE_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	HP_0329

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 260

260

NH(3)-dependent NAD(+) synthetase HAMAP MF_00193

PRO_0000152173

Regions

Nucleotide binding

31 – 38

8

ATP By similarity

Sites

Active site

33

1

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

260

Helix Strand Turn

Details...

Helix

5 – 22

18

Beta strand

28 – 31

4

Helix

36 – 49

14

Helix

50 – 52

3

Beta strand

53 – 57

5

Beta strand

61 – 63

3

Helix

65 – 78

14

Beta strand

82 – 84

3

Helix

88 – 97

10

Helix

103 – 125

23

Beta strand

128 – 130

3

Helix

135 – 140

6

Turn

145 – 149

5

Beta strand

152 – 154

3

Turn

155 – 158

4

Helix

161 – 170

10

Helix

175 – 178

4

Helix

192 – 196

5

Helix

200 – 213

14

Beta strand

214 – 217

4

Helix

221 – 226

6

Helix

231 – 243

13

Helix

245 – 248

4

Sequences

	Sequence		Length	Mass (Da)
	O25096 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 01D8542F2DBF90C8	FASTA	260	29,269
	10 20 30 40 50 60 MQKDYQKLIV YLCDFLEKEV QKRGFKKVVY GLSGGLDSAV VGVLCQKVFK ENAHALLMPS 70 80 90 100 110 120 SVSMPENKTD ALNLCEKFSI PYTEYSIAPY DAIFSSHFKD ASLTRKGNFC ARLRMAFLYD 130 140 150 160 170 180 YSLKSDSLVI GTSNKSERML GYGTLFGDLA CAINPIGELF KTEVYELARR LNIPKKILNK 190 200 210 220 230 240 PPSADLFVGQ SDEKDLGYPY SVIDPLLKDI EALFQTKPID TETLAQLGYD EILVKNITSR 250 260 IQKNAFKLEL PAIAKRFNPE

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000511 Genomic DNA. Translation: AAD07396.1.

PIR

A64561.

RefSeq

NP_207127.1. NC_000915.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XNG	X-ray	1.70	A/B	1-260	[»]
1XNH	X-ray	2.30	A	1-260	[»]

ProteinModelPortal

O25096.

SMR

O25096. Positions 3-260.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

900366.

GenomeReviews

Gene locus HP_0329 in contig AE000511_GR.

KEGG

hpy:HP0329.

NMPDR

fig|85962.1.peg.325.

PATRIC

20591891. VBIHelPyl33062_0343.

TIGR

HP_0329.

Phylogenomic databases

HOGENOM

HBG351567.

OMA

KLIVYLC.

ProtClustDB

PRK13980.

Family and domain databases

HAMAP

MF_00193. NadE.
[Tree]

InterPro

IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]

Gene3D

G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.

KO

K01916.

Pfam

PF02540. NAD_synthase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00552. NadE. 1 hit.

ProtoNet

Search...

Entry information

Entry name

NADE_HELPY

Accession

Primary (citable) accession number: O25096

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program