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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei33 – 331By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 388ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPY:HP0329-MONOMER.
UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetase (EC:6.3.1.5)
Gene namesi
Name:nadE
Ordered Locus Names:HP_0329
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260NH(3)-dependent NAD(+) synthetasePRO_0000152173Add
BLAST

Proteomic databases

PaxDbiO25096.
PRIDEiO25096.

Interactioni

Protein-protein interaction databases

STRINGi85962.HP0329.

Structurei

Secondary structure

1
260
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 2218Combined sources
Beta strandi28 – 314Combined sources
Helixi36 – 4914Combined sources
Helixi50 – 523Combined sources
Beta strandi53 – 575Combined sources
Beta strandi61 – 633Combined sources
Helixi65 – 7814Combined sources
Beta strandi82 – 843Combined sources
Helixi88 – 9710Combined sources
Helixi103 – 12523Combined sources
Beta strandi128 – 1303Combined sources
Helixi135 – 1406Combined sources
Turni145 – 1495Combined sources
Beta strandi152 – 1543Combined sources
Turni155 – 1584Combined sources
Helixi161 – 17010Combined sources
Helixi175 – 1784Combined sources
Beta strandi186 – 1894Combined sources
Helixi192 – 1965Combined sources
Helixi200 – 21314Combined sources
Beta strandi214 – 2174Combined sources
Helixi221 – 2266Combined sources
Helixi231 – 24313Combined sources
Helixi245 – 2484Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XNGX-ray1.70A/B1-260[»]
1XNHX-ray2.30A1-260[»]
ProteinModelPortaliO25096.
SMRiO25096. Positions 3-260.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO25096.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
KOiK01916.
OMAiCARLRMA.
OrthoDBiEOG6VB6S8.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

O25096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKDYQKLIV YLCDFLEKEV QKRGFKKVVY GLSGGLDSAV VGVLCQKVFK
60 70 80 90 100
ENAHALLMPS SVSMPENKTD ALNLCEKFSI PYTEYSIAPY DAIFSSHFKD
110 120 130 140 150
ASLTRKGNFC ARLRMAFLYD YSLKSDSLVI GTSNKSERML GYGTLFGDLA
160 170 180 190 200
CAINPIGELF KTEVYELARR LNIPKKILNK PPSADLFVGQ SDEKDLGYPY
210 220 230 240 250
SVIDPLLKDI EALFQTKPID TETLAQLGYD EILVKNITSR IQKNAFKLEL
260
PAIAKRFNPE
Length:260
Mass (Da):29,269
Last modified:January 1, 1998 - v1
Checksum:i01D8542F2DBF90C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07396.1.
PIRiA64561.
RefSeqiNP_207127.1. NC_000915.1.
WP_001168317.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07396; AAD07396; HP_0329.
GeneIDi900366.
KEGGiheo:C694_01665.
hpy:HP0329.
PATRICi20591891. VBIHelPyl33062_0343.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07396.1.
PIRiA64561.
RefSeqiNP_207127.1. NC_000915.1.
WP_001168317.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XNGX-ray1.70A/B1-260[»]
1XNHX-ray2.30A1-260[»]
ProteinModelPortaliO25096.
SMRiO25096. Positions 3-260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85962.HP0329.

Proteomic databases

PaxDbiO25096.
PRIDEiO25096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07396; AAD07396; HP_0329.
GeneIDi900366.
KEGGiheo:C694_01665.
hpy:HP0329.
PATRICi20591891. VBIHelPyl33062_0343.

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
KOiK01916.
OMAiCARLRMA.
OrthoDBiEOG6VB6S8.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.
BioCyciHPY:HP0329-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO25096.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiNADE_HELPY
AccessioniPrimary (citable) accession number: O25096
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.