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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.UniRule annotation

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi37MagnesiumUniRule annotation1
Binding sitei112Deamido-NADUniRule annotation1
Binding sitei132ATPUniRule annotation1 Publication1
Metal bindingi137MagnesiumUniRule annotation1
Binding sitei161ATPUniRule annotation1 Publication1
Binding sitei183ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 38ATPUniRule annotation1 Publication8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciHPY:HP0329-MONOMER.
UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetase1 PublicationUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:HP_0329
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB04099. Deamido-Nad+.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001521731 – 260NH(3)-dependent NAD(+) synthetaseAdd BLAST260

Proteomic databases

PaxDbiO25096.

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi85962.HP0329.

Structurei

Secondary structure

1260
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 22Combined sources18
Beta strandi28 – 31Combined sources4
Helixi36 – 49Combined sources14
Helixi50 – 52Combined sources3
Beta strandi53 – 57Combined sources5
Beta strandi61 – 63Combined sources3
Helixi65 – 78Combined sources14
Beta strandi82 – 84Combined sources3
Helixi88 – 97Combined sources10
Helixi103 – 125Combined sources23
Beta strandi128 – 130Combined sources3
Helixi135 – 140Combined sources6
Turni145 – 149Combined sources5
Beta strandi152 – 154Combined sources3
Turni155 – 158Combined sources4
Helixi161 – 170Combined sources10
Helixi175 – 178Combined sources4
Beta strandi186 – 189Combined sources4
Helixi192 – 196Combined sources5
Helixi200 – 213Combined sources14
Beta strandi214 – 217Combined sources4
Helixi221 – 226Combined sources6
Helixi231 – 243Combined sources13
Helixi245 – 248Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XNGX-ray1.70A/B1-260[»]
1XNHX-ray2.30A1-260[»]
ProteinModelPortaliO25096.
SMRiO25096.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO25096.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
KOiK01916.
OMAiCAINPIG.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE_ammonia_dep. 1 hit.
InterProiView protein in InterPro
IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR23090. PTHR23090. 1 hit.
PfamiView protein in Pfam
PF02540. NAD_synthase. 1 hit.
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

O25096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKDYQKLIV YLCDFLEKEV QKRGFKKVVY GLSGGLDSAV VGVLCQKVFK
60 70 80 90 100
ENAHALLMPS SVSMPENKTD ALNLCEKFSI PYTEYSIAPY DAIFSSHFKD
110 120 130 140 150
ASLTRKGNFC ARLRMAFLYD YSLKSDSLVI GTSNKSERML GYGTLFGDLA
160 170 180 190 200
CAINPIGELF KTEVYELARR LNIPKKILNK PPSADLFVGQ SDEKDLGYPY
210 220 230 240 250
SVIDPLLKDI EALFQTKPID TETLAQLGYD EILVKNITSR IQKNAFKLEL
260
PAIAKRFNPE
Length:260
Mass (Da):29,269
Last modified:January 1, 1998 - v1
Checksum:i01D8542F2DBF90C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07396.1.
PIRiA64561.
RefSeqiNP_207127.1. NC_000915.1.
WP_001168317.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07396; AAD07396; HP_0329.
GeneIDi900366.
KEGGiheo:C694_01665.
hpy:HP0329.
PATRICifig|85962.47.peg.351.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNADE_HELPY
AccessioniPrimary (citable) accession number: O25096
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: June 7, 2017
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families