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Protein

Pseudaminic acid cytidylyltransferase and UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase

Gene

HP_0326

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the fourth and sixth steps in the biosynthesis of pseudaminic acid, a sialic-acid-like sugar that is used to modify flagellin. The C-terminus mediates the fourth step of the pathway and catalyzes the removal of UDP from C-1 of UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose forming 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose. The N-terminal part mediates the last step of the pathway by mediating activation of pseudaminic acid with CMP by forming CMP-pseudaminic acid.1 Publication

Miscellaneous

In publications, PseF (HP_0326A) and PseG (HP_0326B) are separated into 2 separate ORFs (PubMed:16751642). However, these 2 enzymes are fused into a single protein in strain ATCC 700392 / 26695.1 Publication

Catalytic activityi

UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O = 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + UDP.
CTP + 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid = diphosphate + CMP-5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid.

Cofactori

Mg2+1 PublicationNote: Mg2+ is required for pseudaminic acid cytidylyltransferase activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei244Proton acceptor; for UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciHPY:HP0326-MONOMER
MetaCyc:HP0326-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pseudaminic acid cytidylyltransferase and UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase
Including the following 2 domains:
Alternative name(s):
Pseudaminic acid biosynthesis protein F
Short name:
PseF
Pseudaminic acid cytidylyltransferase HP_0326A
UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase (EC:3.6.1.57)
Alternative name(s):
Pseudaminic acid biosynthesis protein G
Short name:
PseG
UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase HP_0326B
Gene namesi
Ordered Locus Names:HP_0326
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004189361 – 517Pseudaminic acid cytidylyltransferase and UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolaseAdd BLAST517

Proteomic databases

PaxDbiO25093

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

DIPiDIP-3686N
IntActiO25093, 1 interactor
MINTiO25093
STRINGi85962.HP0326

Structurei

3D structure databases

ProteinModelPortaliO25093
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 208Pseudaminic acid cytidylyltransferaseAdd BLAST208
Regioni209 – 517UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolaseAdd BLAST309

Sequence similaritiesi

In the N-terminal section; belongs to the CMP-NeuNAc synthase family.Curated
In the C-terminal section; belongs to the PseG family.Curated

Phylogenomic databases

eggNOGiENOG4105F0R Bacteria
COG1083 LUCA
COG3980 LUCA
KOiK15897
K15899

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR003329 Cytidylyl_trans
IPR029044 Nucleotide-diphossugar_trans
IPR020039 PseF
PfamiView protein in Pfam
PF02348 CTP_transf_3, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR03584 PseF, 1 hit

Sequencei

Sequence statusi: Complete.

O25093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAIAIVLAR SSSKRIKNKN IIDFFNKPML AYPIEVALNS KLFEKVFISS
60 70 80 90 100
DSMEYVNLAK NYGASFLNLR PKILADDRAT TLEVMAYHME ELELKDEDIA
110 120 130 140 150
CCLYGASALL QEKHLKNAFE TLNKNQNTDY VFTCSPFSAS PYRSFSLENG
160 170 180 190 200
VQMAFKEHSN TRTQDLKTLY HDAGLLYMGK AQAFKEMRPI FSQNSIALEL
210 220 230 240 250
SPLEVQDIAH FRRFRISQAQ IQPFEKRMPV KILCDCFLTS GLGHVRRCEK
260 270 280 290 300
ILSFIEKLGV EASLYLHKQN NISAFLEGVG GNDFLITDSY CLNSKDFYLL
310 320 330 340 350
KEKAKSLMVI EDTEHAKGFY PKNTKILNFT LNALKHYHHL SKDYQYYLGV
360 370 380 390 400
GFYPVDARFI YDRPINTENK EVLITLGGSE QKTLKEIVKI LENKNVNLHI
410 420 430 440 450
ISPYTPKNPP KNTHYYSPLN PLEFSSLMKS CACAISAAGQ TLYELALSQT
460 470 480 490 500
PSLILPIASN QIIQSKEFES LGIFKQTSLK TLAKDFENLQ IQKNQAWAKN
510
LVFGDKLEGA LREFLEI
Length:517
Mass (Da):58,934
Last modified:January 1, 1998 - v1
Checksum:iDF78B98ED41F6DA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA Translation: AAD07393.1
PIRiF64560
RefSeqiNP_207124.1, NC_000915.1
WP_001201399.1, NC_018939.1

Genome annotation databases

EnsemblBacteriaiAAD07393; AAD07393; HP_0326
GeneIDi899207
KEGGihpy:HP0326
PATRICifig|85962.8.peg.340

Similar proteinsi

Entry informationi

Entry nameiPSEFG_HELPY
AccessioniPrimary (citable) accession number: O25093
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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