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Protein

Pseudaminic acid cytidylyltransferase and UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase

Gene

HP_0326

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the fourth and sixth steps in the biosynthesis of pseudaminic acid, a sialic-acid-like sugar that is used to modify flagellin. The C-terminus mediates the fourth step of the pathway and catalyzes the removal of UDP from C-1 of UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose forming 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose. The N-terminal part mediates the last step of the pathway by mediating activation of pseudaminic acid with CMP by forming CMP-pseudaminic acid.1 Publication

Catalytic activityi

UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O = 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + UDP.
CTP + 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid = diphosphate + CMP-5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid.

Cofactori

Mg2+1 PublicationNote: Mg2+ is required for pseudaminic acid cytidylyltransferase activity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei244 – 2441Proton acceptor; for UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciHPY:HP0326-MONOMER.
MetaCyc:HP0326-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pseudaminic acid cytidylyltransferase and UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase
Including the following 2 domains:
Alternative name(s):
Pseudaminic acid biosynthesis protein F
Short name:
PseF
Pseudaminic acid cytidylyltransferase HP_0326A
UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase (EC:3.6.1.57)
Alternative name(s):
Pseudaminic acid biosynthesis protein G
Short name:
PseG
UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase HP_0326B
Gene namesi
Ordered Locus Names:HP_0326
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517Pseudaminic acid cytidylyltransferase and UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolasePRO_0000418936Add
BLAST

Proteomic databases

PaxDbiO25093.
PRIDEiO25093.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

DIPiDIP-3686N.
IntActiO25093. 1 interaction.
MINTiMINT-164630.
STRINGi85962.HP0326.

Structurei

3D structure databases

ProteinModelPortaliO25093.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 208208Pseudaminic acid cytidylyltransferaseAdd
BLAST
Regioni209 – 517309UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the CMP-NeuNAc synthase family.Curated
In the C-terminal section; belongs to the PseG family.Curated

Phylogenomic databases

eggNOGiENOG4105F0R. Bacteria.
COG1083. LUCA.
COG3980. LUCA.
KOiK00983.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003329. Cytidylyl_trans.
IPR029044. Nucleotide-diphossugar_trans.
IPR020039. PseF.
[Graphical view]
PfamiPF02348. CTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03584. PseF. 1 hit.

Sequencei

Sequence statusi: Complete.

O25093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAIAIVLAR SSSKRIKNKN IIDFFNKPML AYPIEVALNS KLFEKVFISS
60 70 80 90 100
DSMEYVNLAK NYGASFLNLR PKILADDRAT TLEVMAYHME ELELKDEDIA
110 120 130 140 150
CCLYGASALL QEKHLKNAFE TLNKNQNTDY VFTCSPFSAS PYRSFSLENG
160 170 180 190 200
VQMAFKEHSN TRTQDLKTLY HDAGLLYMGK AQAFKEMRPI FSQNSIALEL
210 220 230 240 250
SPLEVQDIAH FRRFRISQAQ IQPFEKRMPV KILCDCFLTS GLGHVRRCEK
260 270 280 290 300
ILSFIEKLGV EASLYLHKQN NISAFLEGVG GNDFLITDSY CLNSKDFYLL
310 320 330 340 350
KEKAKSLMVI EDTEHAKGFY PKNTKILNFT LNALKHYHHL SKDYQYYLGV
360 370 380 390 400
GFYPVDARFI YDRPINTENK EVLITLGGSE QKTLKEIVKI LENKNVNLHI
410 420 430 440 450
ISPYTPKNPP KNTHYYSPLN PLEFSSLMKS CACAISAAGQ TLYELALSQT
460 470 480 490 500
PSLILPIASN QIIQSKEFES LGIFKQTSLK TLAKDFENLQ IQKNQAWAKN
510
LVFGDKLEGA LREFLEI
Length:517
Mass (Da):58,934
Last modified:January 1, 1998 - v1
Checksum:iDF78B98ED41F6DA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07393.1.
PIRiF64560.
RefSeqiNP_207124.1. NC_000915.1.
WP_001201399.1. NC_000915.1.

Genome annotation databases

EnsemblBacteriaiAAD07393; AAD07393; HP_0326.
GeneIDi899207.
KEGGihpy:HP0326.
PATRICi20591885. VBIHelPyl33062_0340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07393.1.
PIRiF64560.
RefSeqiNP_207124.1. NC_000915.1.
WP_001201399.1. NC_000915.1.

3D structure databases

ProteinModelPortaliO25093.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3686N.
IntActiO25093. 1 interaction.
MINTiMINT-164630.
STRINGi85962.HP0326.

Proteomic databases

PaxDbiO25093.
PRIDEiO25093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07393; AAD07393; HP_0326.
GeneIDi899207.
KEGGihpy:HP0326.
PATRICi20591885. VBIHelPyl33062_0340.

Phylogenomic databases

eggNOGiENOG4105F0R. Bacteria.
COG1083. LUCA.
COG3980. LUCA.
KOiK00983.

Enzyme and pathway databases

BioCyciHPY:HP0326-MONOMER.
MetaCyc:HP0326-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003329. Cytidylyl_trans.
IPR029044. Nucleotide-diphossugar_trans.
IPR020039. PseF.
[Graphical view]
PfamiPF02348. CTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03584. PseF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPSEFG_HELPY
AccessioniPrimary (citable) accession number: O25093
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In publications, PseF (HP_0326A) and PseG (HP_0326B) are separated into 2 separate ORFs (PubMed:16751642). However, these 2 enzymes are fused into a single protein in strain ATCC 700392 / 26695.1 Publication

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.