O25021 (HCPE_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative beta-lactamase HcpE
EC=3.5.2.6

Alternative name(s):
Cysteine-rich protein E

Gene names

Name:	hcpE
Ordered Locus Names:	HP_0235

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May hydrolyze 6-aminopenicillinic acid and 7-aminocephalosporanic acid (ACA) derivatives By similarity.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Secreted Potential.
Miscellaneous	Antibodies against HcpE are present in sera from human patients infected by Helicobacter pylori.
Sequence similarities	Belongs to the hcp beta-lactamase family. Contains 8 TPR repeats.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Secreted
Domain	Repeat Signal TPR repeat
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 355	333	Putative beta-lactamase HcpE		PRO_0000013200
Regions
	Repeat	27 – 60	34	TPR 1
	Repeat	63 – 96	34	TPR 2
	Repeat	98 – 131	34	TPR 3
	Repeat	132 – 166	35	TPR 4
	Repeat	202 – 240	39	TPR 5
	Repeat	245 – 275	31	TPR 6
	Repeat	276 – 311	36	TPR 7
	Repeat	312 – 344	33	TPR 8
Amino acid modifications
	Disulfide bond	54 ↔ 62		Potential
	Disulfide bond	90 ↔ 98		Potential
	Disulfide bond	126 ↔ 134		Potential
	Disulfide bond	160 ↔ 168		Potential
	Disulfide bond	197 ↔ 205		Potential
	Disulfide bond	234 ↔ 242		Potential
	Disulfide bond	270 ↔ 278		Potential
	Disulfide bond	306 ↔ 314		Potential
	Disulfide bond	338 ↔ 346		Potential

Sequences

	Sequence		Length	Mass (Da)
	O25021 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 12B6CB9E295BD628	FASTA	355	39,399
	10 20 30 40 50 60 MGVKFLKILV CGLFFWSLNA HLWGKQDNSF LGVAEKAYKS GNYSKATSYF KKACNDGVSE 70 80 90 100 110 120 GCTQLGIIYE NGQGTRIDYK KALEYYKTAC QADDREGCFG LGGLYDEGLG TTQNYQEAID 130 140 150 160 170 180 AYAKACVLKH PESCYNLGII YDRKIKGNAD QAVTYYQKSC NFDMAKGCYV LGVAYEKGFL 190 200 210 220 230 240 EVKQSNHKAV IYYLKACRLD DGQACRALGS LFENGDAGLD EDFEVAFDYL QKACGLNNSG 250 260 270 280 290 300 GCASLGSMYM LGRYVKKDPQ KAFNFFKQAC DMGSAVSCSR MGFMYSQGDA VPKDLRKALD 310 320 330 340 350 NYERGCDMGD EVGCFALAGM YYNMKDKENA IMIYDKGCKL GMKQACENLT KLRGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[2]	"Detection of high titers of antibody against Helicobacter cysteine-rich proteins A, B, C, and E in Helicobacter pylori-infected individuals." Mittl P.R.E., Luethy L., Reinhardt C., Joller H. Clin. Diagn. Lab. Immunol. 10:542-545(2003) [PubMed] [Europe PMC] [Abstract] Cited for: ANTIGENICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07303.1.
PIR	C64549.
RefSeq	NP_207033.1. NC_000915.1. YP_006934161.1. NC_018939.1.
3D structure databases
ProteinModelPortal	O25021.
ModBase	Search...
Protein-protein interaction databases
STRING	85962.HP0235.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07303; AAD07303; HP_0235.
GeneID	13869418. 898928.
KEGG	heo:C694_01185. hpy:HP0235.
PATRIC	20591695. VBIHelPyl33062_0246.
Phylogenomic databases
eggNOG	COG0790.
KO	K07126.
OMA	KENAIMI.
ProtClustDB	CLSK495931.
Family and domain databases
Gene3D	1.25.40.10. 2 hits.
InterPro	IPR006597. Sel1-like. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF08238. Sel1. 8 hits. [Graphical view]
SMART	SM00671. SEL1. 9 hits. [Graphical view]
PROSITE	PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HCPE_HELPY

Accession

Primary (citable) accession number: O25021

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program