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Protein

Cysteine desulfurase IscS

Gene

iscS

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.UniRule annotation

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei155 – 1551Pyridoxal phosphateUniRule annotation
Binding sitei183 – 1831Pyridoxal phosphateUniRule annotation
Binding sitei241 – 2411Pyridoxal phosphateUniRule annotation
Active sitei328 – 3281Cysteine persulfide intermediateUniRule annotation
Metal bindingi328 – 3281Iron-sulfur (2Fe-2S); via persulfide group; shared with IscUUniRule annotation

GO - Molecular functioni

  1. cysteine desulfurase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. cysteine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate

Enzyme and pathway databases

BioCyciHPY:HP0220-MONOMER.
UniPathwayiUPA00266.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfurase IscSUniRule annotation (EC:2.8.1.7UniRule annotation)
Gene namesi
Name:iscSUniRule annotation
Ordered Locus Names:HP_0220
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Cysteine desulfurase IscSPRO_0000150269Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei206 – 2061N6-(pyridoxal phosphate)lysineUniRule annotation

Proteomic databases

PRIDEiO25008.

Interactioni

Subunit structurei

Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
HP_1262O258523EBI-7607228,EBI-7731253

Protein-protein interaction databases

DIPiDIP-3630N.
IntActiO25008. 5 interactions.
MINTiMINT-177413.
STRINGi85962.HP0220.

Structurei

3D structure databases

ProteinModelPortaliO25008.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni73 – 742Pyridoxal phosphate bindingUniRule annotation
Regioni203 – 2053Pyridoxal phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1104.
KOiK04487.
OMAiRIHRVPN.
OrthoDBiEOG62RSBK.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS.
InterProiIPR000192. Aminotrans_V_dom.
IPR010240. Cys_deSase.
IPR017773. Cys_deSase_NifS_proteobacteria.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR03403. nifS_epsilon. 1 hit.

Sequencei

Sequence statusi: Complete.

O25008-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQRIYLDNN ATTRIDPKVK EIMDPFLRDH YGNPSSLHQF GTETHPAIAE
60 70 80 90 100
ALDKLYKGIN ARDIDDVIIT SCATESNNWV LKGVYFDECL KKGKNHIVTT
110 120 130 140 150
VAEHPAVRST CNFLESLGVE VTYLPINEHG SITAEQVKEA ITEKTALVSV
160 170 180 190 200
MWANNETGLI FPIEEIGAIC KEKGVLFHTD AVQAIGKIPV DVLKANADFL
210 220 230 240 250
SFSAHKFHGP KGIGGLYIRS GVGLTPLFHG GEHMNGRRSG TLNVPYIVGM
260 270 280 290 300
GEAMKLAVEH LDYEKEVVGK LRDKLEEALL KIPDVMVVGD RIHRVPNTTL
310 320 330 340 350
VSVRGIEGEA MLWDLNRSNI AASTGSACAS EDLEANPVMV AIGASKELAH
360 370 380
TAIRLSLSRF NTEAEIDKTI EVFSQAAVRL RNISSSY
Length:387
Mass (Da):42,404
Last modified:December 31, 1997 - v1
Checksum:i93AF9C7FB6FC8D94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07288.1.
PIRiD64547.
RefSeqiNP_207018.1. NC_000915.1.
YP_006934146.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07288; AAD07288; HP_0220.
GeneIDi13869402.
900131.
KEGGiheo:C694_01110.
hpy:HP0220.
PATRICi20591663. VBIHelPyl33062_0231.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07288.1.
PIRiD64547.
RefSeqiNP_207018.1. NC_000915.1.
YP_006934146.1. NC_018939.1.

3D structure databases

ProteinModelPortaliO25008.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3630N.
IntActiO25008. 5 interactions.
MINTiMINT-177413.
STRINGi85962.HP0220.

Proteomic databases

PRIDEiO25008.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07288; AAD07288; HP_0220.
GeneIDi13869402.
900131.
KEGGiheo:C694_01110.
hpy:HP0220.
PATRICi20591663. VBIHelPyl33062_0231.

Phylogenomic databases

eggNOGiCOG1104.
KOiK04487.
OMAiRIHRVPN.
OrthoDBiEOG62RSBK.

Enzyme and pathway databases

UniPathwayiUPA00266.
BioCyciHPY:HP0220-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS.
InterProiIPR000192. Aminotrans_V_dom.
IPR010240. Cys_deSase.
IPR017773. Cys_deSase_NifS_proteobacteria.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR03403. nifS_epsilon. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiISCS_HELPY
AccessioniPrimary (citable) accession number: O25008
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2001
Last sequence update: December 31, 1997
Last modified: March 3, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.