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O25008 (ISCS_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cysteine desulfurase IscS

EC=2.8.1.7
Gene names
Name:iscS
Ordered Locus Names:HP_0220
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins By similarity. HAMAP-Rule MF_00331

Catalytic activity

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor. HAMAP-Rule MF_00331

Cofactor

Pyridoxal phosphate By similarity. HAMAP-Rule MF_00331

Subunit structure

Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00331.

Sequence similarities

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandPyridoxal phosphate
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcysteine metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine desulfurase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 387387Cysteine desulfurase IscS HAMAP-Rule MF_00331
PRO_0000150269

Sites

Active site3281Cysteine persulfide intermediate By similarity

Amino acid modifications

Modified residue2061N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
O25008 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 93AF9C7FB6FC8D94

FASTA38742,404
        10         20         30         40         50         60 
MLQRIYLDNN ATTRIDPKVK EIMDPFLRDH YGNPSSLHQF GTETHPAIAE ALDKLYKGIN 

        70         80         90        100        110        120 
ARDIDDVIIT SCATESNNWV LKGVYFDECL KKGKNHIVTT VAEHPAVRST CNFLESLGVE 

       130        140        150        160        170        180 
VTYLPINEHG SITAEQVKEA ITEKTALVSV MWANNETGLI FPIEEIGAIC KEKGVLFHTD 

       190        200        210        220        230        240 
AVQAIGKIPV DVLKANADFL SFSAHKFHGP KGIGGLYIRS GVGLTPLFHG GEHMNGRRSG 

       250        260        270        280        290        300 
TLNVPYIVGM GEAMKLAVEH LDYEKEVVGK LRDKLEEALL KIPDVMVVGD RIHRVPNTTL 

       310        320        330        340        350        360 
VSVRGIEGEA MLWDLNRSNI AASTGSACAS EDLEANPVMV AIGASKELAH TAIRLSLSRF 

       370        380 
NTEAEIDKTI EVFSQAAVRL RNISSSY 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000511 Genomic DNA. Translation: AAD07288.1.
PIRD64547.
RefSeqNP_207018.1. NC_000915.1.
YP_006934146.1. NC_018939.1.

3D structure databases

ProteinModelPortalO25008.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-3630N.
IntActO25008. 1 interaction.
MINTMINT-177413.
STRING85962.HP0220.

Proteomic databases

PRIDEO25008.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07288; AAD07288; HP_0220.
GeneID13869402.
900131.
KEGGheo:C694_01110.
hpy:HP0220.
PATRIC20591663. VBIHelPyl33062_0231.

Phylogenomic databases

eggNOGCOG1104.
KOK04487.
OMARIHRVPN.
OrthoDBEOG62RSBK.
ProtClustDBCLSK872301.

Enzyme and pathway databases

BioCycHPY:HP0220-MONOMER.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPMF_00331. Cys_desulf_aminotr_5.
InterProIPR000192. Aminotrans_V/Cys_dSase.
IPR010240. Cys_deSase.
IPR017773. Cys_deSase_NifS_proteobacteria.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFPIRSF005572. NifS. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
TIGRFAMsTIGR03403. nifS_epsilon. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISCS_HELPY
AccessionPrimary (citable) accession number: O25008
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names