O25008 (ISCS_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine desulfurase
EC=2.8.1.7

Gene names

Name:	iscS
Ordered Locus Names:	HP_0220

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the removal of elemental sulfur from cysteine to produce alanine By similarity. HAMAP-Rule MF_00331
Catalytic activity	L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor. HAMAP-Rule MF_00331
Cofactor	Pyridoxal phosphate By similarity.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cysteine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine desulfurase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Cysteine desulfurase HAMAP-Rule MF_00331		PRO_0000150269
Sites
	Active site	328	1	Cysteine persulfide intermediate By similarity
Amino acid modifications
	Modified residue	206	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O25008 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 93AF9C7FB6FC8D94	FASTA	387	42,404
	10 20 30 40 50 60 MLQRIYLDNN ATTRIDPKVK EIMDPFLRDH YGNPSSLHQF GTETHPAIAE ALDKLYKGIN 70 80 90 100 110 120 ARDIDDVIIT SCATESNNWV LKGVYFDECL KKGKNHIVTT VAEHPAVRST CNFLESLGVE 130 140 150 160 170 180 VTYLPINEHG SITAEQVKEA ITEKTALVSV MWANNETGLI FPIEEIGAIC KEKGVLFHTD 190 200 210 220 230 240 AVQAIGKIPV DVLKANADFL SFSAHKFHGP KGIGGLYIRS GVGLTPLFHG GEHMNGRRSG 250 260 270 280 290 300 TLNVPYIVGM GEAMKLAVEH LDYEKEVVGK LRDKLEEALL KIPDVMVVGD RIHRVPNTTL 310 320 330 340 350 360 VSVRGIEGEA MLWDLNRSNI AASTGSACAS EDLEANPVMV AIGASKELAH TAIRLSLSRF 370 380 NTEAEIDKTI EVFSQAAVRL RNISSSY

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07288.1.
PIR	D64547.
RefSeq	NP_207018.1. NC_000915.1. YP_006934146.1. NC_018939.1.
3D structure databases
ProteinModelPortal	O25008.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3630N.
MINT	MINT-177413.
STRING	85962.HP0220.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07288; AAD07288; HP_0220.
GeneID	13869402. 900131.
KEGG	heo:C694_01110. hpy:HP0220.
PATRIC	20591663. VBIHelPyl33062_0231.
Phylogenomic databases
eggNOG	COG1104.
KO	K04487.
OMA	CAHGSIR.
ProtClustDB	CLSK872301.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
HAMAP	MF_00331. Cys_desulf_aminotr_5.
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR010240. Cys_deSase. IPR017773. Cys_deSase_NifS_proteobacteria. IPR016454. Cysteine_dSase_NifS. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
PIRSF	PIRSF005572. NifS. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR03403. nifS_epsilon. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISCS_HELPY

Accession

Primary (citable) accession number: O25008

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program