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O25008

- ISCS_HELPY

UniProt

O25008 - ISCS_HELPY

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Protein

Cysteine desulfurase IscS

Gene
iscS, HP_0220
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins By similarity.UniRule annotation

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.UniRule annotation

Cofactori

Pyridoxal phosphate By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei328 – 3281Cysteine persulfide intermediate By similarity

GO - Molecular functioni

  1. cysteine desulfurase activity Source: UniProtKB-HAMAP
  2. protein binding Source: IntAct
  3. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. cysteine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciHPY:HP0220-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfurase IscS (EC:2.8.1.7)
Gene namesi
Name:iscS
Ordered Locus Names:HP_0220
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387Cysteine desulfurase IscSUniRule annotationPRO_0000150269Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei206 – 2061N6-(pyridoxal phosphate)lysine By similarity

Proteomic databases

PRIDEiO25008.

Interactioni

Subunit structurei

Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
HP_1262O258523EBI-7607228,EBI-7731253

Protein-protein interaction databases

DIPiDIP-3630N.
IntActiO25008. 5 interactions.
MINTiMINT-177413.
STRINGi85962.HP0220.

Structurei

3D structure databases

ProteinModelPortaliO25008.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1104.
KOiK04487.
OMAiPATLEVC.
OrthoDBiEOG62RSBK.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS.
InterProiIPR000192. Aminotrans_V/Cys_dSase.
IPR010240. Cys_deSase.
IPR017773. Cys_deSase_NifS_proteobacteria.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR03403. nifS_epsilon. 1 hit.

Sequencei

Sequence statusi: Complete.

O25008-1 [UniParc]FASTAAdd to Basket

« Hide

MLQRIYLDNN ATTRIDPKVK EIMDPFLRDH YGNPSSLHQF GTETHPAIAE    50
ALDKLYKGIN ARDIDDVIIT SCATESNNWV LKGVYFDECL KKGKNHIVTT 100
VAEHPAVRST CNFLESLGVE VTYLPINEHG SITAEQVKEA ITEKTALVSV 150
MWANNETGLI FPIEEIGAIC KEKGVLFHTD AVQAIGKIPV DVLKANADFL 200
SFSAHKFHGP KGIGGLYIRS GVGLTPLFHG GEHMNGRRSG TLNVPYIVGM 250
GEAMKLAVEH LDYEKEVVGK LRDKLEEALL KIPDVMVVGD RIHRVPNTTL 300
VSVRGIEGEA MLWDLNRSNI AASTGSACAS EDLEANPVMV AIGASKELAH 350
TAIRLSLSRF NTEAEIDKTI EVFSQAAVRL RNISSSY 387
Length:387
Mass (Da):42,404
Last modified:January 1, 1998 - v1
Checksum:i93AF9C7FB6FC8D94
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD07288.1.
PIRiD64547.
RefSeqiNP_207018.1. NC_000915.1.
YP_006934146.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07288; AAD07288; HP_0220.
GeneIDi13869402.
900131.
KEGGiheo:C694_01110.
hpy:HP0220.
PATRICi20591663. VBIHelPyl33062_0231.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD07288.1 .
PIRi D64547.
RefSeqi NP_207018.1. NC_000915.1.
YP_006934146.1. NC_018939.1.

3D structure databases

ProteinModelPortali O25008.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-3630N.
IntActi O25008. 5 interactions.
MINTi MINT-177413.
STRINGi 85962.HP0220.

Proteomic databases

PRIDEi O25008.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD07288 ; AAD07288 ; HP_0220 .
GeneIDi 13869402.
900131.
KEGGi heo:C694_01110.
hpy:HP0220.
PATRICi 20591663. VBIHelPyl33062_0231.

Phylogenomic databases

eggNOGi COG1104.
KOi K04487.
OMAi PATLEVC.
OrthoDBi EOG62RSBK.

Enzyme and pathway databases

BioCyci HPY:HP0220-MONOMER.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPi MF_00331. Cys_desulf_IscS.
InterProi IPR000192. Aminotrans_V/Cys_dSase.
IPR010240. Cys_deSase.
IPR017773. Cys_deSase_NifS_proteobacteria.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00266. Aminotran_5. 1 hit.
[Graphical view ]
PIRSFi PIRSF005572. NifS. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR03403. nifS_epsilon. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.

Entry informationi

Entry nameiISCS_HELPY
AccessioniPrimary (citable) accession number: O25008
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi