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O25008

- ISCS_HELPY

UniProt

O25008 - ISCS_HELPY

Protein

Cysteine desulfurase IscS

Gene

iscS

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.UniRule annotation

    Catalytic activityi

    L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei328 – 3281Cysteine persulfide intermediateUniRule annotation

    GO - Molecular functioni

    1. cysteine desulfurase activity Source: UniProtKB-HAMAP
    2. protein binding Source: IntAct
    3. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. cysteine metabolic process Source: InterPro

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciHPY:HP0220-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine desulfurase IscSUniRule annotation (EC:2.8.1.7UniRule annotation)
    Gene namesi
    Name:iscSUniRule annotation
    Ordered Locus Names:HP_0220
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 387387Cysteine desulfurase IscSPRO_0000150269Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei206 – 2061N6-(pyridoxal phosphate)lysineUniRule annotation

    Proteomic databases

    PRIDEiO25008.

    Interactioni

    Subunit structurei

    Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.UniRule annotation

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HP_1262O258523EBI-7607228,EBI-7731253

    Protein-protein interaction databases

    DIPiDIP-3630N.
    IntActiO25008. 5 interactions.
    MINTiMINT-177413.
    STRINGi85962.HP0220.

    Structurei

    3D structure databases

    ProteinModelPortaliO25008.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1104.
    KOiK04487.
    OMAiPATLEVC.
    OrthoDBiEOG62RSBK.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPiMF_00331. Cys_desulf_IscS.
    InterProiIPR000192. Aminotrans_V/Cys_dSase.
    IPR010240. Cys_deSase.
    IPR017773. Cys_deSase_NifS_proteobacteria.
    IPR016454. Cysteine_dSase_NifS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00266. Aminotran_5. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005572. NifS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR03403. nifS_epsilon. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O25008-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLQRIYLDNN ATTRIDPKVK EIMDPFLRDH YGNPSSLHQF GTETHPAIAE    50
    ALDKLYKGIN ARDIDDVIIT SCATESNNWV LKGVYFDECL KKGKNHIVTT 100
    VAEHPAVRST CNFLESLGVE VTYLPINEHG SITAEQVKEA ITEKTALVSV 150
    MWANNETGLI FPIEEIGAIC KEKGVLFHTD AVQAIGKIPV DVLKANADFL 200
    SFSAHKFHGP KGIGGLYIRS GVGLTPLFHG GEHMNGRRSG TLNVPYIVGM 250
    GEAMKLAVEH LDYEKEVVGK LRDKLEEALL KIPDVMVVGD RIHRVPNTTL 300
    VSVRGIEGEA MLWDLNRSNI AASTGSACAS EDLEANPVMV AIGASKELAH 350
    TAIRLSLSRF NTEAEIDKTI EVFSQAAVRL RNISSSY 387
    Length:387
    Mass (Da):42,404
    Last modified:January 1, 1998 - v1
    Checksum:i93AF9C7FB6FC8D94
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07288.1.
    PIRiD64547.
    RefSeqiNP_207018.1. NC_000915.1.
    YP_006934146.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD07288; AAD07288; HP_0220.
    GeneIDi13869402.
    900131.
    KEGGiheo:C694_01110.
    hpy:HP0220.
    PATRICi20591663. VBIHelPyl33062_0231.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07288.1 .
    PIRi D64547.
    RefSeqi NP_207018.1. NC_000915.1.
    YP_006934146.1. NC_018939.1.

    3D structure databases

    ProteinModelPortali O25008.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-3630N.
    IntActi O25008. 5 interactions.
    MINTi MINT-177413.
    STRINGi 85962.HP0220.

    Proteomic databases

    PRIDEi O25008.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD07288 ; AAD07288 ; HP_0220 .
    GeneIDi 13869402.
    900131.
    KEGGi heo:C694_01110.
    hpy:HP0220.
    PATRICi 20591663. VBIHelPyl33062_0231.

    Phylogenomic databases

    eggNOGi COG1104.
    KOi K04487.
    OMAi PATLEVC.
    OrthoDBi EOG62RSBK.

    Enzyme and pathway databases

    BioCyci HPY:HP0220-MONOMER.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    HAMAPi MF_00331. Cys_desulf_IscS.
    InterProi IPR000192. Aminotrans_V/Cys_dSase.
    IPR010240. Cys_deSase.
    IPR017773. Cys_deSase_NifS_proteobacteria.
    IPR016454. Cysteine_dSase_NifS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00266. Aminotran_5. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005572. NifS. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR03403. nifS_epsilon. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.

    Entry informationi

    Entry nameiISCS_HELPY
    AccessioniPrimary (citable) accession number: O25008
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3