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O25001

- HCPA_HELPY

UniProt

O25001 - HCPA_HELPY

Protein

Beta-lactamase HcpA

Gene

hcpA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Slowly hydrolyzes 6-aminopenicillinic acid and 7-aminocephalosporanic acid (ACA) derivatives. May be involved in the synthesis of the cell wall peptidoglycan.1 Publication

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.

    Enzyme regulationi

    Inhibited by cloxacillin and oxacillin but not by ACA derivatives or metal chelators.1 Publication

    GO - Molecular functioni

    1. beta-lactamase activity Source: CACAO

    GO - Biological processi

    1. metabolic process Source: GOC
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    BioCyciHPY:HP0211-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase HcpA (EC:3.5.2.6)
    Alternative name(s):
    Cysteine-rich 28 kDa protein
    Gene namesi
    Name:hcpA
    Ordered Locus Names:HP_0211
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 250225Beta-lactamase HcpAPRO_0000013194Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi56 ↔ 641 Publication
    Disulfide bondi92 ↔ 1001 Publication
    Disulfide bondi128 ↔ 1361 Publication
    Disulfide bondi164 ↔ 1721 Publication
    Disulfide bondi196 ↔ 2041 Publication
    Disulfide bondi232 ↔ 240Sequence Analysis

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiO25001.

    Interactioni

    Protein-protein interaction databases

    STRINGi85962.HP0211.

    Structurei

    3D structure databases

    ProteinModelPortaliO25001.
    SMRiO25001. Positions 28-250.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati29 – 6234TPR 1Add
    BLAST
    Repeati67 – 9832TPR 2Add
    BLAST
    Repeati100 – 13334TPR 3Add
    BLAST
    Repeati134 – 16936TPR 4Add
    BLAST
    Repeati170 – 20233TPR 5Add
    BLAST

    Sequence similaritiesi

    Belongs to the hcp beta-lactamase family.Curated
    Contains 5 TPR repeats.Curated

    Keywords - Domaini

    Repeat, Signal, TPR repeat

    Phylogenomic databases

    eggNOGiCOG0790.
    KOiK07126.
    OMAiHAEGCMV.
    OrthoDBiEOG6TJ7WZ.

    Family and domain databases

    Gene3Di1.25.40.10. 1 hit.
    InterProiIPR006597. Sel1-like.
    IPR011990. TPR-like_helical.
    IPR013105. TPR_2.
    [Graphical view]
    PfamiPF08238. Sel1. 5 hits.
    PF07719. TPR_2. 1 hit.
    [Graphical view]
    SMARTiSM00671. SEL1. 6 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O25001-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLGNVKKTLF GVLCLGTLCL RGLMAEPDAK ELVNLGIESA KKQDFAQAKT    50
    HFEKACELKN GFGCVFLGAF YEEGKGVGKD LKKAIQFYTK GCELNDGYGC 100
    NLLGNLYYNG QGVSKDAKKA SQYYSKACDL NHAEGCMVLG SLHHYGVGTP 150
    KDLRKALDLY EKACDLKDSP GCINAGYIYS VTKNFKEAIV RYSKACELKD 200
    GRGCYNLGVM QYNAQGTAKD EKQAVENFKK GCKSSVKEAC DALKELKIEL 250
    Length:250
    Mass (Da):27,366
    Last modified:January 1, 1998 - v1
    Checksum:iA375FF12EB795355
    GO

    Mass spectrometryi

    Molecular mass is 25613±1.6 Da from positions 26 - 250. Determined by MALDI. 1 Publication

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti4 – 41N → S in strain: ATCC 49503.
    Natural varianti9 – 91L → F in strain: ATCC 49503.
    Natural varianti11 – 111G → W in strain: ATCC 49503.
    Natural varianti17 – 171T → A in strain: ATCC 49503.
    Natural varianti91 – 911G → S in strain: ATCC 49503.
    Natural varianti194 – 1941K → Q in strain: ATCC 49503.
    Natural varianti199 – 1991K → N in strain: ATCC 49503.
    Natural varianti235 – 2351S → G in strain: ATCC 49503.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF008565 Genomic DNA. Translation: AAB63298.1.
    AF053708 Genomic DNA. Translation: AAC24210.1.
    AE000511 Genomic DNA. Translation: AAD07279.1.
    PIRiC64546.
    RefSeqiNP_207009.1. NC_000915.1.
    YP_006934136.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD07279; AAD07279; HP_0211.
    GeneIDi13869392.
    899162.
    KEGGiheo:C694_01060.
    hpy:HP0211.
    PATRICi20591645. VBIHelPyl33062_0222.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF008565 Genomic DNA. Translation: AAB63298.1 .
    AF053708 Genomic DNA. Translation: AAC24210.1 .
    AE000511 Genomic DNA. Translation: AAD07279.1 .
    PIRi C64546.
    RefSeqi NP_207009.1. NC_000915.1.
    YP_006934136.1. NC_018939.1.

    3D structure databases

    ProteinModelPortali O25001.
    SMRi O25001. Positions 28-250.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 85962.HP0211.

    Proteomic databases

    PRIDEi O25001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD07279 ; AAD07279 ; HP_0211 .
    GeneIDi 13869392.
    899162.
    KEGGi heo:C694_01060.
    hpy:HP0211.
    PATRICi 20591645. VBIHelPyl33062_0222.

    Phylogenomic databases

    eggNOGi COG0790.
    KOi K07126.
    OMAi HAEGCMV.
    OrthoDBi EOG6TJ7WZ.

    Enzyme and pathway databases

    BioCyci HPY:HP0211-MONOMER.

    Family and domain databases

    Gene3Di 1.25.40.10. 1 hit.
    InterProi IPR006597. Sel1-like.
    IPR011990. TPR-like_helical.
    IPR013105. TPR_2.
    [Graphical view ]
    Pfami PF08238. Sel1. 5 hits.
    PF07719. TPR_2. 1 hit.
    [Graphical view ]
    SMARTi SM00671. SEL1. 6 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of Helicobacter pylori dapE and construction of a conditionally lethal dapE mutant."
      Karita M., Etterbeek M.L., Forsyth M.H., Tummuru M.K.R., Blaser M.J.
      Infect. Immun. 65:4158-4164(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 49503 / 60190.
    2. "Extracellular release of antigenic proteins by Helicobacter pylori."
      Cao P., McClain M.S., Forsyth M.H., Cover T.L.
      Infect. Immun. 66:2984-2986(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
      Strain: ATCC 49503 / 60190.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.
    4. "The cysteine-rich protein A from Helicobacter pylori is a beta-lactamase."
      Mittl P.R.E., Luethy L., Hunziker P., Gruetter M.G.
      J. Biol. Chem. 275:17693-17699(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, MASS SPECTROMETRY, DISULFIDE BONDS.
    5. "Detection of high titers of antibody against Helicobacter cysteine-rich proteins A, B, C, and E in Helicobacter pylori-infected individuals."
      Mittl P.R.E., Luethy L., Reinhardt C., Joller H.
      Clin. Diagn. Lab. Immunol. 10:542-545(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ANTIGENICITY.

    Entry informationi

    Entry nameiHCPA_HELPY
    AccessioniPrimary (citable) accession number: O25001
    Secondary accession number(s): O32634, O85223
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Antibodies against HcpA are present in sera from human patients infected by Helicobacter pylori.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3