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O25001 (HCPA_HELPY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase HcpA

EC=3.5.2.6
Alternative name(s):
Cysteine-rich 28 kDa protein
Gene names
Name:hcpA
Ordered Locus Names:HP_0211
OrganismHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]
Taxonomic identifier85962 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Slowly hydrolyzes 6-aminopenicillinic acid and 7-aminocephalosporanic acid (ACA) derivatives. May be involved in the synthesis of the cell wall peptidoglycan. Ref.4

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Enzyme regulation

Inhibited by cloxacillin and oxacillin but not by ACA derivatives or metal chelators. Ref.4

Subcellular location

Secreted Ref.2.

Miscellaneous

Antibodies against HcpA are present in sera from human patients infected by Helicobacter pylori.

Sequence similarities

Belongs to the hcp beta-lactamase family.

Contains 5 TPR repeats.

Mass spectrometry

Molecular mass is 25613±1.6 Da from positions 26 - 250. Determined by MALDI. Ref.4

Ontologies

Keywords
   Biological processAntibiotic resistance
   Cellular componentSecreted
   DomainRepeat
Signal
TPR repeat
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmetabolic process

Inferred from direct assay Ref.4. Source: GOC

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-lactamase activity

Inferred from direct assay Ref.4. Source: CACAO

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 250225Beta-lactamase HcpA
PRO_0000013194

Regions

Repeat29 – 6234TPR 1
Repeat67 – 9832TPR 2
Repeat100 – 13334TPR 3
Repeat134 – 16936TPR 4
Repeat170 – 20233TPR 5

Amino acid modifications

Disulfide bond56 ↔ 64 Ref.4
Disulfide bond92 ↔ 100 Ref.4
Disulfide bond128 ↔ 136 Ref.4
Disulfide bond164 ↔ 172 Ref.4
Disulfide bond196 ↔ 204 Ref.4
Disulfide bond232 ↔ 240 Potential

Natural variations

Natural variant41N → S in strain: ATCC 49503.
Natural variant91L → F in strain: ATCC 49503.
Natural variant111G → W in strain: ATCC 49503.
Natural variant171T → A in strain: ATCC 49503.
Natural variant911G → S in strain: ATCC 49503.
Natural variant1941K → Q in strain: ATCC 49503.
Natural variant1991K → N in strain: ATCC 49503.
Natural variant2351S → G in strain: ATCC 49503.

Sequences

Sequence LengthMass (Da)Tools
O25001 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: A375FF12EB795355

FASTA25027,366
        10         20         30         40         50         60 
MLGNVKKTLF GVLCLGTLCL RGLMAEPDAK ELVNLGIESA KKQDFAQAKT HFEKACELKN 

        70         80         90        100        110        120 
GFGCVFLGAF YEEGKGVGKD LKKAIQFYTK GCELNDGYGC NLLGNLYYNG QGVSKDAKKA 

       130        140        150        160        170        180 
SQYYSKACDL NHAEGCMVLG SLHHYGVGTP KDLRKALDLY EKACDLKDSP GCINAGYIYS 

       190        200        210        220        230        240 
VTKNFKEAIV RYSKACELKD GRGCYNLGVM QYNAQGTAKD EKQAVENFKK GCKSSVKEAC 

       250 
DALKELKIEL 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of Helicobacter pylori dapE and construction of a conditionally lethal dapE mutant."
Karita M., Etterbeek M.L., Forsyth M.H., Tummuru M.K.R., Blaser M.J.
Infect. Immun. 65:4158-4164(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49503 / 60190.
[2]"Extracellular release of antigenic proteins by Helicobacter pylori."
Cao P., McClain M.S., Forsyth M.H., Cover T.L.
Infect. Immun. 66:2984-2986(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
Strain: ATCC 49503 / 60190.
[3]"The complete genome sequence of the gastric pathogen Helicobacter pylori."
Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. expand/collapse author list , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.
Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700392 / 26695.
[4]"The cysteine-rich protein A from Helicobacter pylori is a beta-lactamase."
Mittl P.R.E., Luethy L., Hunziker P., Gruetter M.G.
J. Biol. Chem. 275:17693-17699(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION, MASS SPECTROMETRY, DISULFIDE BONDS.
[5]"Detection of high titers of antibody against Helicobacter cysteine-rich proteins A, B, C, and E in Helicobacter pylori-infected individuals."
Mittl P.R.E., Luethy L., Reinhardt C., Joller H.
Clin. Diagn. Lab. Immunol. 10:542-545(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ANTIGENICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF008565 Genomic DNA. Translation: AAB63298.1.
AF053708 Genomic DNA. Translation: AAC24210.1.
AE000511 Genomic DNA. Translation: AAD07279.1.
PIRC64546.
RefSeqNP_207009.1. NC_000915.1.
YP_006934136.1. NC_018939.1.

3D structure databases

ProteinModelPortalO25001.
SMRO25001. Positions 28-250.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING85962.HP0211.

Proteomic databases

PRIDEO25001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD07279; AAD07279; HP_0211.
GeneID13869392.
899162.
KEGGheo:C694_01060.
hpy:HP0211.
PATRIC20591645. VBIHelPyl33062_0222.

Phylogenomic databases

eggNOGCOG0790.
KOK07126.
OMATHFEKAC.
OrthoDBEOG6TJ7WZ.
ProtClustDBCLSK2392992.

Enzyme and pathway databases

BioCycHPY:HP0211-MONOMER.

Family and domain databases

Gene3D1.25.40.10. 1 hit.
InterProIPR006597. Sel1-like.
IPR011990. TPR-like_helical.
IPR013105. TPR_2.
[Graphical view]
PfamPF08238. Sel1. 5 hits.
PF07719. TPR_2. 1 hit.
[Graphical view]
SMARTSM00671. SEL1. 6 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHCPA_HELPY
AccessionPrimary (citable) accession number: O25001
Secondary accession number(s): O32634, O85223
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names