O25001 (HCPA_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase HcpA
EC=3.5.2.6

Alternative name(s):
Cysteine-rich 28 kDa protein

Gene names

Name:	hcpA
Ordered Locus Names:	HP_0211

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Slowly hydrolyzes 6-aminopenicillinic acid and 7-aminocephalosporanic acid (ACA) derivatives. May be involved in the synthesis of the cell wall peptidoglycan. Ref.4
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Enzyme regulation	Inhibited by cloxacillin and oxacillin but not by ACA derivatives or metal chelators. Ref.4
Subcellular location	Secreted Ref.2.
Miscellaneous	Antibodies against HcpA are present in sera from human patients infected by Helicobacter pylori.
Sequence similarities	Belongs to the hcp beta-lactamase family. Contains 5 TPR repeats.
Mass spectrometry	Molecular mass is 25613±1.6 Da from positions 26 - 250. Determined by MALDI. Ref.4

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Secreted
Domain	Repeat Signal TPR repeat
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 250	225	Beta-lactamase HcpA		PRO_0000013194
Regions
	Repeat	29 – 62	34	TPR 1
	Repeat	67 – 98	32	TPR 2
	Repeat	100 – 133	34	TPR 3
	Repeat	134 – 169	36	TPR 4
	Repeat	170 – 202	33	TPR 5
Amino acid modifications
	Disulfide bond	56 ↔ 64		Ref.4
	Disulfide bond	92 ↔ 100		Ref.4
	Disulfide bond	128 ↔ 136		Ref.4
	Disulfide bond	164 ↔ 172		Ref.4
	Disulfide bond	196 ↔ 204		Ref.4
	Disulfide bond	232 ↔ 240		Potential
Natural variations
	Natural variant	4	1	N → S in strain: ATCC 49503.
	Natural variant	9	1	L → F in strain: ATCC 49503.
	Natural variant	11	1	G → W in strain: ATCC 49503.
	Natural variant	17	1	T → A in strain: ATCC 49503.
	Natural variant	91	1	G → S in strain: ATCC 49503.
	Natural variant	194	1	K → Q in strain: ATCC 49503.
	Natural variant	199	1	K → N in strain: ATCC 49503.
	Natural variant	235	1	S → G in strain: ATCC 49503.

Sequences

	Sequence		Length	Mass (Da)
	O25001 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A375FF12EB795355	FASTA	250	27,366
	10 20 30 40 50 60 MLGNVKKTLF GVLCLGTLCL RGLMAEPDAK ELVNLGIESA KKQDFAQAKT HFEKACELKN 70 80 90 100 110 120 GFGCVFLGAF YEEGKGVGKD LKKAIQFYTK GCELNDGYGC NLLGNLYYNG QGVSKDAKKA 130 140 150 160 170 180 SQYYSKACDL NHAEGCMVLG SLHHYGVGTP KDLRKALDLY EKACDLKDSP GCINAGYIYS 190 200 210 220 230 240 VTKNFKEAIV RYSKACELKD GRGCYNLGVM QYNAQGTAKD EKQAVENFKK GCKSSVKEAC 250 DALKELKIEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of Helicobacter pylori dapE and construction of a conditionally lethal dapE mutant." Karita M., Etterbeek M.L., Forsyth M.H., Tummuru M.K.R., Blaser M.J. Infect. Immun. 65:4158-4164(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49503 / 60190.
[2]	"Extracellular release of antigenic proteins by Helicobacter pylori." Cao P., McClain M.S., Forsyth M.H., Cover T.L. Infect. Immun. 66:2984-2986(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION. Strain: ATCC 49503 / 60190.
[3]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[4]	"The cysteine-rich protein A from Helicobacter pylori is a beta-lactamase." Mittl P.R.E., Luethy L., Hunziker P., Gruetter M.G. J. Biol. Chem. 275:17693-17699(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ENZYME REGULATION, MASS SPECTROMETRY, DISULFIDE BONDS.
[5]	"Detection of high titers of antibody against Helicobacter cysteine-rich proteins A, B, C, and E in Helicobacter pylori-infected individuals." Mittl P.R.E., Luethy L., Reinhardt C., Joller H. Clin. Diagn. Lab. Immunol. 10:542-545(2003) [PubMed] [Europe PMC] [Abstract] Cited for: ANTIGENICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF008565 Genomic DNA. Translation: AAB63298.1. AF053708 Genomic DNA. Translation: AAC24210.1. AE000511 Genomic DNA. Translation: AAD07279.1.
PIR	C64546.
RefSeq	NP_207009.1. NC_000915.1. YP_006934136.1. NC_018939.1.
3D structure databases
ProteinModelPortal	O25001.
SMR	O25001. Positions 28-250.
ModBase	Search...
Protein-protein interaction databases
STRING	85962.HP0211.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07279; AAD07279; HP_0211.
GeneID	13869392. 899162.
KEGG	heo:C694_01060. hpy:HP0211.
PATRIC	20591645. VBIHelPyl33062_0222.
Phylogenomic databases
eggNOG	COG0790.
KO	K07126.
OMA	THFEKAC.
ProtClustDB	CLSK2392992.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR006597. Sel1-like. IPR011990. TPR-like_helical. IPR013105. TPR_2. [Graphical view]
Pfam	PF08238. Sel1. 5 hits. PF07719. TPR_2. 1 hit. [Graphical view]
SMART	SM00671. SEL1. 6 hits. [Graphical view]
PROSITE	PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HCPA_HELPY

Accession

Primary (citable) accession number: O25001
Secondary accession number(s): O32634, O85223

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program