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O25001

- HCPA_HELPY

UniProt

O25001 - HCPA_HELPY

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Protein

Beta-lactamase HcpA

Gene

hcpA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Slowly hydrolyzes 6-aminopenicillinic acid and 7-aminocephalosporanic acid (ACA) derivatives. May be involved in the synthesis of the cell wall peptidoglycan.1 Publication

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Enzyme regulationi

Inhibited by cloxacillin and oxacillin but not by ACA derivatives or metal chelators.1 Publication

GO - Molecular functioni

  1. beta-lactamase activity Source: CACAO

GO - Biological processi

  1. metabolic process Source: GOC
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BioCyciHPY:HP0211-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase HcpA (EC:3.5.2.6)
Alternative name(s):
Cysteine-rich 28 kDa protein
Gene namesi
Name:hcpA
Ordered Locus Names:HP_0211
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 250225Beta-lactamase HcpAPRO_0000013194Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 641 Publication
Disulfide bondi92 ↔ 1001 Publication
Disulfide bondi128 ↔ 1361 Publication
Disulfide bondi164 ↔ 1721 Publication
Disulfide bondi196 ↔ 2041 Publication
Disulfide bondi232 ↔ 240Sequence Analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO25001.

Interactioni

Protein-protein interaction databases

STRINGi85962.HP0211.

Structurei

3D structure databases

ProteinModelPortaliO25001.
SMRiO25001. Positions 28-250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati29 – 6234TPR 1Add
BLAST
Repeati67 – 9832TPR 2Add
BLAST
Repeati100 – 13334TPR 3Add
BLAST
Repeati134 – 16936TPR 4Add
BLAST
Repeati170 – 20233TPR 5Add
BLAST

Sequence similaritiesi

Belongs to the hcp beta-lactamase family.Curated
Contains 5 TPR repeats.Curated

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiCOG0790.
KOiK07126.
OMAiHAEGCMV.
OrthoDBiEOG6TJ7WZ.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR006597. Sel1-like.
IPR011990. TPR-like_helical_dom.
IPR013105. TPR_2.
[Graphical view]
PfamiPF08238. Sel1. 5 hits.
PF07719. TPR_2. 1 hit.
[Graphical view]
SMARTiSM00671. SEL1. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O25001-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLGNVKKTLF GVLCLGTLCL RGLMAEPDAK ELVNLGIESA KKQDFAQAKT
60 70 80 90 100
HFEKACELKN GFGCVFLGAF YEEGKGVGKD LKKAIQFYTK GCELNDGYGC
110 120 130 140 150
NLLGNLYYNG QGVSKDAKKA SQYYSKACDL NHAEGCMVLG SLHHYGVGTP
160 170 180 190 200
KDLRKALDLY EKACDLKDSP GCINAGYIYS VTKNFKEAIV RYSKACELKD
210 220 230 240 250
GRGCYNLGVM QYNAQGTAKD EKQAVENFKK GCKSSVKEAC DALKELKIEL
Length:250
Mass (Da):27,366
Last modified:January 1, 1998 - v1
Checksum:iA375FF12EB795355
GO

Mass spectrometryi

Molecular mass is 25613±1.6 Da from positions 26 - 250. Determined by MALDI. 1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti4 – 41N → S in strain: ATCC 49503.
Natural varianti9 – 91L → F in strain: ATCC 49503.
Natural varianti11 – 111G → W in strain: ATCC 49503.
Natural varianti17 – 171T → A in strain: ATCC 49503.
Natural varianti91 – 911G → S in strain: ATCC 49503.
Natural varianti194 – 1941K → Q in strain: ATCC 49503.
Natural varianti199 – 1991K → N in strain: ATCC 49503.
Natural varianti235 – 2351S → G in strain: ATCC 49503.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008565 Genomic DNA. Translation: AAB63298.1.
AF053708 Genomic DNA. Translation: AAC24210.1.
AE000511 Genomic DNA. Translation: AAD07279.1.
PIRiC64546.
RefSeqiNP_207009.1. NC_000915.1.
YP_006934136.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07279; AAD07279; HP_0211.
GeneIDi13869392.
899162.
KEGGiheo:C694_01060.
hpy:HP0211.
PATRICi20591645. VBIHelPyl33062_0222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008565 Genomic DNA. Translation: AAB63298.1 .
AF053708 Genomic DNA. Translation: AAC24210.1 .
AE000511 Genomic DNA. Translation: AAD07279.1 .
PIRi C64546.
RefSeqi NP_207009.1. NC_000915.1.
YP_006934136.1. NC_018939.1.

3D structure databases

ProteinModelPortali O25001.
SMRi O25001. Positions 28-250.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 85962.HP0211.

Proteomic databases

PRIDEi O25001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD07279 ; AAD07279 ; HP_0211 .
GeneIDi 13869392.
899162.
KEGGi heo:C694_01060.
hpy:HP0211.
PATRICi 20591645. VBIHelPyl33062_0222.

Phylogenomic databases

eggNOGi COG0790.
KOi K07126.
OMAi HAEGCMV.
OrthoDBi EOG6TJ7WZ.

Enzyme and pathway databases

BioCyci HPY:HP0211-MONOMER.

Family and domain databases

Gene3Di 1.25.40.10. 1 hit.
InterProi IPR006597. Sel1-like.
IPR011990. TPR-like_helical_dom.
IPR013105. TPR_2.
[Graphical view ]
Pfami PF08238. Sel1. 5 hits.
PF07719. TPR_2. 1 hit.
[Graphical view ]
SMARTi SM00671. SEL1. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of Helicobacter pylori dapE and construction of a conditionally lethal dapE mutant."
    Karita M., Etterbeek M.L., Forsyth M.H., Tummuru M.K.R., Blaser M.J.
    Infect. Immun. 65:4158-4164(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49503 / 60190.
  2. "Extracellular release of antigenic proteins by Helicobacter pylori."
    Cao P., McClain M.S., Forsyth M.H., Cover T.L.
    Infect. Immun. 66:2984-2986(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
    Strain: ATCC 49503 / 60190.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.
  4. "The cysteine-rich protein A from Helicobacter pylori is a beta-lactamase."
    Mittl P.R.E., Luethy L., Hunziker P., Gruetter M.G.
    J. Biol. Chem. 275:17693-17699(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, MASS SPECTROMETRY, DISULFIDE BONDS.
  5. "Detection of high titers of antibody against Helicobacter cysteine-rich proteins A, B, C, and E in Helicobacter pylori-infected individuals."
    Mittl P.R.E., Luethy L., Reinhardt C., Joller H.
    Clin. Diagn. Lab. Immunol. 10:542-545(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: ANTIGENICITY.

Entry informationi

Entry nameiHCPA_HELPY
AccessioniPrimary (citable) accession number: O25001
Secondary accession number(s): O32634, O85223
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Antibodies against HcpA are present in sera from human patients infected by Helicobacter pylori.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3