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Protein

Beta-lactamase HcpA

Gene

hcpA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Slowly hydrolyzes 6-aminopenicillinic acid and 7-aminocephalosporanic acid (ACA) derivatives. May be involved in the synthesis of the cell wall peptidoglycan.1 Publication

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Enzyme regulationi

Inhibited by cloxacillin and oxacillin but not by ACA derivatives or metal chelators.1 Publication

GO - Molecular functioni

  • beta-lactamase activity Source: CACAO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BioCyciHPY:HP0211-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase HcpA (EC:3.5.2.6)
Alternative name(s):
Cysteine-rich 28 kDa protein
Gene namesi
Name:hcpA
Ordered Locus Names:HP_0211
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000001319426 – 250Beta-lactamase HcpAAdd BLAST225

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi56 ↔ 641 Publication
Disulfide bondi92 ↔ 1001 Publication
Disulfide bondi128 ↔ 1361 Publication
Disulfide bondi164 ↔ 1721 Publication
Disulfide bondi196 ↔ 2041 Publication
Disulfide bondi232 ↔ 240Sequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO25001.

Interactioni

Protein-protein interaction databases

STRINGi85962.HP0211.

Structurei

3D structure databases

ProteinModelPortaliO25001.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati29 – 62TPR 1Add BLAST34
Repeati67 – 98TPR 2Add BLAST32
Repeati100 – 133TPR 3Add BLAST34
Repeati134 – 169TPR 4Add BLAST36
Repeati170 – 202TPR 5Add BLAST33

Sequence similaritiesi

Belongs to the hcp beta-lactamase family.Curated
Contains 5 TPR repeats.Curated

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiENOG4105EUU. Bacteria.
COG0790. LUCA.
KOiK07126.
OMAiYRSCKLA.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR006597. Sel1-like.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF08238. Sel1. 4 hits.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00671. SEL1. 6 hits.
SM00028. TPR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O25001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGNVKKTLF GVLCLGTLCL RGLMAEPDAK ELVNLGIESA KKQDFAQAKT
60 70 80 90 100
HFEKACELKN GFGCVFLGAF YEEGKGVGKD LKKAIQFYTK GCELNDGYGC
110 120 130 140 150
NLLGNLYYNG QGVSKDAKKA SQYYSKACDL NHAEGCMVLG SLHHYGVGTP
160 170 180 190 200
KDLRKALDLY EKACDLKDSP GCINAGYIYS VTKNFKEAIV RYSKACELKD
210 220 230 240 250
GRGCYNLGVM QYNAQGTAKD EKQAVENFKK GCKSSVKEAC DALKELKIEL
Length:250
Mass (Da):27,366
Last modified:January 1, 1998 - v1
Checksum:iA375FF12EB795355
GO

Mass spectrometryi

Molecular mass is 25613±1.6 Da from positions 26 - 250. Determined by MALDI. 1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti4N → S in strain: ATCC 49503. 1
Natural varianti9L → F in strain: ATCC 49503. 1
Natural varianti11G → W in strain: ATCC 49503. 1
Natural varianti17T → A in strain: ATCC 49503. 1
Natural varianti91G → S in strain: ATCC 49503. 1
Natural varianti194K → Q in strain: ATCC 49503. 1
Natural varianti199K → N in strain: ATCC 49503. 1
Natural varianti235S → G in strain: ATCC 49503. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008565 Genomic DNA. Translation: AAB63298.1.
AF053708 Genomic DNA. Translation: AAC24210.1.
AE000511 Genomic DNA. Translation: AAD07279.1.
PIRiC64546.
RefSeqiNP_207009.1. NC_000915.1.
WP_000901420.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07279; AAD07279; HP_0211.
GeneIDi899162.
KEGGiheo:C694_01060.
hpy:HP0211.
PATRICi20591645. VBIHelPyl33062_0222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008565 Genomic DNA. Translation: AAB63298.1.
AF053708 Genomic DNA. Translation: AAC24210.1.
AE000511 Genomic DNA. Translation: AAD07279.1.
PIRiC64546.
RefSeqiNP_207009.1. NC_000915.1.
WP_000901420.1. NC_018939.1.

3D structure databases

ProteinModelPortaliO25001.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85962.HP0211.

Proteomic databases

PaxDbiO25001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07279; AAD07279; HP_0211.
GeneIDi899162.
KEGGiheo:C694_01060.
hpy:HP0211.
PATRICi20591645. VBIHelPyl33062_0222.

Phylogenomic databases

eggNOGiENOG4105EUU. Bacteria.
COG0790. LUCA.
KOiK07126.
OMAiYRSCKLA.

Enzyme and pathway databases

BioCyciHPY:HP0211-MONOMER.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR006597. Sel1-like.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF08238. Sel1. 4 hits.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00671. SEL1. 6 hits.
SM00028. TPR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHCPA_HELPY
AccessioniPrimary (citable) accession number: O25001
Secondary accession number(s): O32634, O85223
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 1, 1998
Last modified: October 5, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Antibodies against HcpA are present in sera from human patients infected by Helicobacter pylori.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.