ID   FABI_HELPY              Reviewed;         275 AA.
AC   O24990;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 65.
DE   RecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH];
DE            EC=1.3.1.9;
DE   AltName: Full=NADH-dependent enoyl-ACP reductase;
GN   Name=fabI; OrderedLocusNames=HP_0195;
OS   Helicobacter pylori (Campylobacter pylori).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=210;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700392 / 26695;
RX   MEDLINE=97394467; PubMed=9252185; DOI=10.1038/41483;
RA   Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA   Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R.,
RA   Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F.,
RA   Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G.,
RA   Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D.,
RA   Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D.,
RA   Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C.,
RA   Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D.,
RA   Smith H.O., Fraser C.M., Venter J.C.;
RT   "The complete genome sequence of the gastric pathogen Helicobacter
RT   pylori.";
RL   Nature 388:539-547(1997).
CC   -!- CATALYTIC ACTIVITY: Acyl-[acyl-carrier-protein] + NAD(+) = trans-
CC       2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
CC   -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane
CC       protein (By similarity).
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family. FabI subfamily.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AE000511; AAD07262.1; -; Genomic_DNA.
DR   PIR; C64544; C64544.
DR   RefSeq; NP_206994.1; -.
DR   PDB; 2PD3; X-ray; 2.50 A; A/B/C/D=1-275.
DR   PDB; 2PD4; X-ray; 2.30 A; A/B/C/D=1-275.
DR   PDBsum; 2PD3; -.
DR   PDBsum; 2PD4; -.
DR   GeneID; 899156; -.
DR   GenomeReviews; AE000511_GR; HP_0195.
DR   KEGG; hpy:HP0195; -.
DR   NMPDR; fig|85962.1.peg.192; -.
DR   TIGR; HP_0195; -.
DR   HOGENOM; O24990; -.
DR   OMA; O24990; LVHCLAF.
DR   BRENDA; 1.3.1.9; 1131.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NAD...; IEA:EC.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR014358; Enoyl-ACP_Rdtase_NADH.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   PANTHER; PTHR19410:SF12; Enoyl-ACP_rdct; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Complete proteome;
KW   Fatty acid biosynthesis; Lipid synthesis; Membrane; NAD;
KW   Oxidoreductase.
FT   CHAIN         1    275       Enoyl-[acyl-carrier-protein] reductase
FT                                [NADH].
FT                                /FTId=PRO_0000054902.
FT   NP_BIND      10     36       NAD (By similarity).
FT   ACT_SITE    155    155       Proton acceptor (By similarity).
FT   TURN          3      6
FT   STRAND        8     12
FT   HELIX        20     29
FT   TURN         30     32
FT   STRAND       34     41
FT   TURN         42     44
FT   HELIX        45     54
FT   STRAND       60     62
FT   HELIX        68     81
FT   STRAND       85     90
FT   HELIX        97    100
FT   HELIX       104    106
FT   HELIX       109    119
FT   HELIX       121    130
FT   HELIX       131    133
FT   STRAND      134    144
FT   HELIX       146    148
FT   HELIX       156    177
FT   TURN        178    180
FT   STRAND      182    188
FT   HELIX       196    198
FT   HELIX       202    212
FT   HELIX       221    232
FT   HELIX       234    236
FT   STRAND      243    247
FT   HELIX       250    252
FT   STRAND      253    255
FT   TURN        262    265
FT   HELIX       268    271
SQ   SEQUENCE   275 AA;  29981 MW;  1894A5AA2105895A CRC64;
     MGFLKGKKGL IVGVANNKSI AYGIAQSCFN QGATLAFTYL NESLEKRVRP IAQELNSPYV
     YELDVSKEEH FKSLYNSVKK DLGSLDFIVH SVAFAPKEAL EGSLLETSKS AFNTAMEISV
     YSLIELTNTL KPLLNNGASV LTLSYLGSTK YMAHYNVMGL AKAALESAVR YLAVDLGKHH
     IRVNALSAGP IRTLASSGIA DFRMILKWNE INAPLRKNVS LEEVGNAGMY LLSSLSSGVS
     GEVHFVDAGY HVMGMGAVEE KDNKATLLWD LHKEQ
//