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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathway:ifatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NAD; via carbonyl oxygen1 Publication
Binding sitei92 – 921NAD; via carbonyl oxygen1 Publication
Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygen
Active sitei145 – 1451Proton acceptorBy similarity
Active sitei155 – 1551Proton acceptor
Binding sitei162 – 1621NAD1 Publication
Sitei203 – 2031Involved in acyl-ACP bindingBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 202NAD1 Publication
Nucleotide bindingi64 – 652NAD1 Publication
Nucleotide bindingi191 – 1955NAD1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciHPY:HP0195-MONOMER.
BRENDAi1.3.1.9. 2604.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:HP_0195
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429 Componenti: Chromosome

Pathology & Biotechi

Chemistry

DrugBankiDB08604. Triclosan.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054902Add
BLAST

Proteomic databases

PRIDEiO24990.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

IntActiO24990. 1 interaction.
STRINGi85962.HP0195.

Structurei

Secondary structure

1
275
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni3 – 64Combined sources
Beta strandi8 – 125Combined sources
Helixi20 – 2910Combined sources
Turni30 – 323Combined sources
Beta strandi34 – 418Combined sources
Turni42 – 443Combined sources
Helixi45 – 5410Combined sources
Beta strandi60 – 623Combined sources
Helixi68 – 8114Combined sources
Beta strandi85 – 906Combined sources
Helixi97 – 1004Combined sources
Helixi104 – 1063Combined sources
Helixi109 – 11911Combined sources
Helixi121 – 13010Combined sources
Helixi131 – 1333Combined sources
Beta strandi134 – 14411Combined sources
Helixi146 – 1483Combined sources
Helixi156 – 17722Combined sources
Turni178 – 1803Combined sources
Beta strandi182 – 1887Combined sources
Helixi196 – 1983Combined sources
Helixi202 – 21211Combined sources
Helixi221 – 23212Combined sources
Helixi234 – 2363Combined sources
Beta strandi243 – 2475Combined sources
Helixi250 – 2523Combined sources
Beta strandi253 – 2553Combined sources
Turni262 – 2654Combined sources
Helixi268 – 2714Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PD3X-ray2.50A/B/C/D1-275[»]
2PD4X-ray2.30A/B/C/D1-275[»]
ProteinModelPortaliO24990.
SMRiO24990. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO24990.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiGILDMIH.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

O24990-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLKGKKGL IVGVANNKSI AYGIAQSCFN QGATLAFTYL NESLEKRVRP
60 70 80 90 100
IAQELNSPYV YELDVSKEEH FKSLYNSVKK DLGSLDFIVH SVAFAPKEAL
110 120 130 140 150
EGSLLETSKS AFNTAMEISV YSLIELTNTL KPLLNNGASV LTLSYLGSTK
160 170 180 190 200
YMAHYNVMGL AKAALESAVR YLAVDLGKHH IRVNALSAGP IRTLASSGIA
210 220 230 240 250
DFRMILKWNE INAPLRKNVS LEEVGNAGMY LLSSLSSGVS GEVHFVDAGY
260 270
HVMGMGAVEE KDNKATLLWD LHKEQ
Length:275
Mass (Da):29,981
Last modified:January 1, 1998 - v1
Checksum:i1894A5AA2105895A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07262.1.
PIRiC64544.
RefSeqiNP_206994.1. NC_000915.1.
WP_000506216.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07262; AAD07262; HP_0195.
GeneIDi899156.
KEGGiheo:C694_00970.
hpy:HP0195.
PATRICi20591611. VBIHelPyl33062_0205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07262.1.
PIRiC64544.
RefSeqiNP_206994.1. NC_000915.1.
WP_000506216.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PD3X-ray2.50A/B/C/D1-275[»]
2PD4X-ray2.30A/B/C/D1-275[»]
ProteinModelPortaliO24990.
SMRiO24990. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO24990. 1 interaction.
STRINGi85962.HP0195.

Chemistry

DrugBankiDB08604. Triclosan.

Proteomic databases

PRIDEiO24990.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07262; AAD07262; HP_0195.
GeneIDi899156.
KEGGiheo:C694_00970.
hpy:HP0195.
PATRICi20591611. VBIHelPyl33062_0205.

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiGILDMIH.
OrthoDBiEOG6HF644.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciHPY:HP0195-MONOMER.
BRENDAi1.3.1.9. 2604.

Miscellaneous databases

EvolutionaryTraceiO24990.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.
  2. "Crystal structure of the Helicobacter pylori enoyl-acyl carrier protein reductase in complex with hydroxydiphenyl ether compounds, triclosan and diclosan."
    Lee H.H., Moon J., Suh S.W.
    Proteins 69:691-694(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH NAD AND INHIBITORS, SUBUNIT.

Entry informationi

Entry nameiFABI_HELPY
AccessioniPrimary (citable) accession number: O24990
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: July 22, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.