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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13NAD; via carbonyl oxygen1 Publication1
Binding sitei92NAD; via carbonyl oxygen1 Publication1
Binding sitei95Substrate; via amide nitrogen and carbonyl oxygen1
Active sitei145Proton acceptorBy similarity1
Active sitei155Proton acceptor1
Binding sitei162NAD1 Publication1
Sitei203Involved in acyl-ACP bindingBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 20NAD1 Publication2
Nucleotide bindingi64 – 65NAD1 Publication2
Nucleotide bindingi191 – 195NAD1 Publication5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciHPY:HP0195-MONOMER.
BRENDAi1.3.1.9. 2604.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:HP_0195
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB08604. Triclosan.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549021 – 275Enoyl-[acyl-carrier-protein] reductase [NADH] FabIAdd BLAST275

Proteomic databases

PaxDbiO24990.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

IntActiO24990. 1 interactor.
STRINGi85962.HP0195.

Structurei

Secondary structure

1275
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni3 – 6Combined sources4
Beta strandi8 – 12Combined sources5
Helixi20 – 29Combined sources10
Turni30 – 32Combined sources3
Beta strandi34 – 41Combined sources8
Turni42 – 44Combined sources3
Helixi45 – 54Combined sources10
Beta strandi60 – 62Combined sources3
Helixi68 – 81Combined sources14
Beta strandi85 – 90Combined sources6
Helixi97 – 100Combined sources4
Helixi104 – 106Combined sources3
Helixi109 – 119Combined sources11
Helixi121 – 130Combined sources10
Helixi131 – 133Combined sources3
Beta strandi134 – 144Combined sources11
Helixi146 – 148Combined sources3
Helixi156 – 177Combined sources22
Turni178 – 180Combined sources3
Beta strandi182 – 188Combined sources7
Helixi196 – 198Combined sources3
Helixi202 – 212Combined sources11
Helixi221 – 232Combined sources12
Helixi234 – 236Combined sources3
Beta strandi243 – 247Combined sources5
Helixi250 – 252Combined sources3
Beta strandi253 – 255Combined sources3
Turni262 – 265Combined sources4
Helixi268 – 271Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PD3X-ray2.50A/B/C/D1-275[»]
2PD4X-ray2.30A/B/C/D1-275[»]
ProteinModelPortaliO24990.
SMRiO24990.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO24990.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CSJ. Bacteria.
COG0623. LUCA.
KOiK00208.
OMAiAGYCING.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF317. PTHR24322:SF317. 2 hits.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

O24990-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLKGKKGL IVGVANNKSI AYGIAQSCFN QGATLAFTYL NESLEKRVRP
60 70 80 90 100
IAQELNSPYV YELDVSKEEH FKSLYNSVKK DLGSLDFIVH SVAFAPKEAL
110 120 130 140 150
EGSLLETSKS AFNTAMEISV YSLIELTNTL KPLLNNGASV LTLSYLGSTK
160 170 180 190 200
YMAHYNVMGL AKAALESAVR YLAVDLGKHH IRVNALSAGP IRTLASSGIA
210 220 230 240 250
DFRMILKWNE INAPLRKNVS LEEVGNAGMY LLSSLSSGVS GEVHFVDAGY
260 270
HVMGMGAVEE KDNKATLLWD LHKEQ
Length:275
Mass (Da):29,981
Last modified:January 1, 1998 - v1
Checksum:i1894A5AA2105895A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07262.1.
PIRiC64544.
RefSeqiNP_206994.1. NC_000915.1.
WP_000506216.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07262; AAD07262; HP_0195.
GeneIDi899156.
KEGGiheo:C694_00970.
hpy:HP0195.
PATRICi20591611. VBIHelPyl33062_0205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA. Translation: AAD07262.1.
PIRiC64544.
RefSeqiNP_206994.1. NC_000915.1.
WP_000506216.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PD3X-ray2.50A/B/C/D1-275[»]
2PD4X-ray2.30A/B/C/D1-275[»]
ProteinModelPortaliO24990.
SMRiO24990.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO24990. 1 interactor.
STRINGi85962.HP0195.

Chemistry databases

DrugBankiDB08604. Triclosan.

Proteomic databases

PaxDbiO24990.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07262; AAD07262; HP_0195.
GeneIDi899156.
KEGGiheo:C694_00970.
hpy:HP0195.
PATRICi20591611. VBIHelPyl33062_0205.

Phylogenomic databases

eggNOGiENOG4105CSJ. Bacteria.
COG0623. LUCA.
KOiK00208.
OMAiAGYCING.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciHPY:HP0195-MONOMER.
BRENDAi1.3.1.9. 2604.

Miscellaneous databases

EvolutionaryTraceiO24990.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF317. PTHR24322:SF317. 2 hits.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFABI_HELPY
AccessioniPrimary (citable) accession number: O24990
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.