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O24990

- FABI_HELPY

UniProt

O24990 - FABI_HELPY

Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism By similarity.By similarity

    Catalytic activityi

    An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131NAD; via carbonyl oxygen1 Publication
    Binding sitei92 – 921NAD; via carbonyl oxygen1 Publication
    Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygen
    Active sitei145 – 1451Proton acceptorBy similarity
    Active sitei155 – 1551Proton acceptor
    Binding sitei162 – 1621NAD1 Publication
    Sitei203 – 2031Involved in acyl-ACP bindingBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 202NAD1 Publication
    Nucleotide bindingi64 – 652NAD1 Publication
    Nucleotide bindingi191 – 1955NAD1 Publication

    GO - Molecular functioni

    1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB

    GO - Biological processi

    1. fatty acid elongation Source: UniProtKB
    2. protein homotetramerization Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciHPY:HP0195-MONOMER.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
    Short name:
    ENR
    Alternative name(s):
    NADH-dependent enoyl-ACP reductase
    Gene namesi
    Name:fabI
    Ordered Locus Names:HP_0195
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 275275Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054902Add
    BLAST

    Proteomic databases

    PRIDEiO24990.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    IntActiO24990. 1 interaction.
    STRINGi85962.HP0195.

    Structurei

    Secondary structure

    1
    275
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni3 – 64
    Beta strandi8 – 125
    Helixi20 – 2910
    Turni30 – 323
    Beta strandi34 – 418
    Turni42 – 443
    Helixi45 – 5410
    Beta strandi60 – 623
    Helixi68 – 8114
    Beta strandi85 – 906
    Helixi97 – 1004
    Helixi104 – 1063
    Helixi109 – 11911
    Helixi121 – 13010
    Helixi131 – 1333
    Beta strandi134 – 14411
    Helixi146 – 1483
    Helixi156 – 17722
    Turni178 – 1803
    Beta strandi182 – 1887
    Helixi196 – 1983
    Helixi202 – 21211
    Helixi221 – 23212
    Helixi234 – 2363
    Beta strandi243 – 2475
    Helixi250 – 2523
    Beta strandi253 – 2553
    Turni262 – 2654
    Helixi268 – 2714

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2PD3X-ray2.50A/B/C/D1-275[»]
    2PD4X-ray2.30A/B/C/D1-275[»]
    ProteinModelPortaliO24990.
    SMRiO24990. Positions 2-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO24990.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0623.
    KOiK00208.
    OMAiQHWDKFD.
    OrthoDBiEOG6HF644.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
    PRINTSiPR00081. GDHRDH.

    Sequencei

    Sequence statusi: Complete.

    O24990-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFLKGKKGL IVGVANNKSI AYGIAQSCFN QGATLAFTYL NESLEKRVRP    50
    IAQELNSPYV YELDVSKEEH FKSLYNSVKK DLGSLDFIVH SVAFAPKEAL 100
    EGSLLETSKS AFNTAMEISV YSLIELTNTL KPLLNNGASV LTLSYLGSTK 150
    YMAHYNVMGL AKAALESAVR YLAVDLGKHH IRVNALSAGP IRTLASSGIA 200
    DFRMILKWNE INAPLRKNVS LEEVGNAGMY LLSSLSSGVS GEVHFVDAGY 250
    HVMGMGAVEE KDNKATLLWD LHKEQ 275
    Length:275
    Mass (Da):29,981
    Last modified:January 1, 1998 - v1
    Checksum:i1894A5AA2105895A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07262.1.
    PIRiC64544.
    RefSeqiNP_206994.1. NC_000915.1.
    YP_006934118.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD07262; AAD07262; HP_0195.
    GeneIDi13869374.
    899156.
    KEGGiheo:C694_00970.
    hpy:HP0195.
    PATRICi20591611. VBIHelPyl33062_0205.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000511 Genomic DNA. Translation: AAD07262.1 .
    PIRi C64544.
    RefSeqi NP_206994.1. NC_000915.1.
    YP_006934118.1. NC_018939.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2PD3 X-ray 2.50 A/B/C/D 1-275 [» ]
    2PD4 X-ray 2.30 A/B/C/D 1-275 [» ]
    ProteinModelPortali O24990.
    SMRi O24990. Positions 2-275.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O24990. 1 interaction.
    STRINGi 85962.HP0195.

    Proteomic databases

    PRIDEi O24990.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD07262 ; AAD07262 ; HP_0195 .
    GeneIDi 13869374.
    899156.
    KEGGi heo:C694_00970.
    hpy:HP0195.
    PATRICi 20591611. VBIHelPyl33062_0205.

    Phylogenomic databases

    eggNOGi COG0623.
    KOi K00208.
    OMAi QHWDKFD.
    OrthoDBi EOG6HF644.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .
    BioCyci HPY:HP0195-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei O24990.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
    PRINTSi PR00081. GDHRDH.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.
    2. "Crystal structure of the Helicobacter pylori enoyl-acyl carrier protein reductase in complex with hydroxydiphenyl ether compounds, triclosan and diclosan."
      Lee H.H., Moon J., Suh S.W.
      Proteins 69:691-694(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH NAD AND INHIBITORS, SUBUNIT.

    Entry informationi

    Entry nameiFABI_HELPY
    AccessioniPrimary (citable) accession number: O24990
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3