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O24990

- FABI_HELPY

UniProt

O24990 - FABI_HELPY

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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene
fabI, HP_0195
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism By similarity.

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NAD; via carbonyl oxygen
Binding sitei92 – 921NAD; via carbonyl oxygen
Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygen
Active sitei145 – 1451Proton acceptor By similarity
Active sitei155 – 1551Proton acceptor
Binding sitei162 – 1621NAD
Sitei203 – 2031Involved in acyl-ACP binding By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 202NAD
Nucleotide bindingi64 – 652NAD
Nucleotide bindingi191 – 1955NAD

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB

GO - Biological processi

  1. fatty acid elongation Source: UniProtKB
  2. protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciHPY:HP0195-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:HP_0195
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054902Add
BLAST

Proteomic databases

PRIDEiO24990.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

IntActiO24990. 1 interaction.
STRINGi85962.HP0195.

Structurei

Secondary structure

1
275
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni3 – 64
Beta strandi8 – 125
Helixi20 – 2910
Turni30 – 323
Beta strandi34 – 418
Turni42 – 443
Helixi45 – 5410
Beta strandi60 – 623
Helixi68 – 8114
Beta strandi85 – 906
Helixi97 – 1004
Helixi104 – 1063
Helixi109 – 11911
Helixi121 – 13010
Helixi131 – 1333
Beta strandi134 – 14411
Helixi146 – 1483
Helixi156 – 17722
Turni178 – 1803
Beta strandi182 – 1887
Helixi196 – 1983
Helixi202 – 21211
Helixi221 – 23212
Helixi234 – 2363
Beta strandi243 – 2475
Helixi250 – 2523
Beta strandi253 – 2553
Turni262 – 2654
Helixi268 – 2714

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PD3X-ray2.50A/B/C/D1-275[»]
2PD4X-ray2.30A/B/C/D1-275[»]
ProteinModelPortaliO24990.
SMRiO24990. Positions 2-275.

Miscellaneous databases

EvolutionaryTraceiO24990.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiQHWDKFD.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

O24990-1 [UniParc]FASTAAdd to Basket

« Hide

MGFLKGKKGL IVGVANNKSI AYGIAQSCFN QGATLAFTYL NESLEKRVRP    50
IAQELNSPYV YELDVSKEEH FKSLYNSVKK DLGSLDFIVH SVAFAPKEAL 100
EGSLLETSKS AFNTAMEISV YSLIELTNTL KPLLNNGASV LTLSYLGSTK 150
YMAHYNVMGL AKAALESAVR YLAVDLGKHH IRVNALSAGP IRTLASSGIA 200
DFRMILKWNE INAPLRKNVS LEEVGNAGMY LLSSLSSGVS GEVHFVDAGY 250
HVMGMGAVEE KDNKATLLWD LHKEQ 275
Length:275
Mass (Da):29,981
Last modified:January 1, 1998 - v1
Checksum:i1894A5AA2105895A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD07262.1.
PIRiC64544.
RefSeqiNP_206994.1. NC_000915.1.
YP_006934118.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07262; AAD07262; HP_0195.
GeneIDi13869374.
899156.
KEGGiheo:C694_00970.
hpy:HP0195.
PATRICi20591611. VBIHelPyl33062_0205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000511 Genomic DNA. Translation: AAD07262.1 .
PIRi C64544.
RefSeqi NP_206994.1. NC_000915.1.
YP_006934118.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2PD3 X-ray 2.50 A/B/C/D 1-275 [» ]
2PD4 X-ray 2.30 A/B/C/D 1-275 [» ]
ProteinModelPortali O24990.
SMRi O24990. Positions 2-275.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O24990. 1 interaction.
STRINGi 85962.HP0195.

Proteomic databases

PRIDEi O24990.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD07262 ; AAD07262 ; HP_0195 .
GeneIDi 13869374.
899156.
KEGGi heo:C694_00970.
hpy:HP0195.
PATRICi 20591611. VBIHelPyl33062_0205.

Phylogenomic databases

eggNOGi COG0623.
KOi K00208.
OMAi QHWDKFD.
OrthoDBi EOG6HF644.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci HPY:HP0195-MONOMER.

Miscellaneous databases

EvolutionaryTracei O24990.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSi PR00081. GDHRDH.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.
  2. "Crystal structure of the Helicobacter pylori enoyl-acyl carrier protein reductase in complex with hydroxydiphenyl ether compounds, triclosan and diclosan."
    Lee H.H., Moon J., Suh S.W.
    Proteins 69:691-694(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH NAD AND INHIBITORS, SUBUNIT.

Entry informationi

Entry nameiFABI_HELPY
AccessioniPrimary (citable) accession number: O24990
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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