O24968 (HCPD_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative beta-lactamase HcpD
EC=3.5.2.6

Alternative name(s):
Cysteine-rich protein D
Penicillin-binding protein 4
Short name=PBP 4

Gene names

Name:	hcpD
Ordered Locus Names:	HP_0160

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May hydrolyze 6-aminopenicillinic acid and 7-aminocephalosporanic acid (ACA) derivatives By similarity. Binds to penicillin.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the hcp beta-lactamase family. Contains 4 TPR repeats.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Secreted
Domain	Repeat Signal TPR repeat
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 306	281	Putative beta-lactamase HcpD		PRO_0000013198
Regions
	Repeat	28 – 61	34	TPR 1
	Repeat	96 – 133	38	TPR 2
	Repeat	168 – 205	38	TPR 3
	Repeat	240 – 277	38	TPR 4
Amino acid modifications
	Disulfide bond	55 ↔ 63		Potential
	Disulfide bond	91 ↔ 99		Potential
	Disulfide bond	127 ↔ 135		Potential
	Disulfide bond	163 ↔ 171		Potential
	Disulfide bond	199 ↔ 207		Potential
	Disulfide bond	235 ↔ 243		Potential
	Disulfide bond	271 ↔ 279		Potential

Sequences

	Sequence		Length	Mass (Da)
	O24968 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: D3BDE25CA3009B87	FASTA	306	34,097
	10 20 30 40 50 60 MIKSWTKKWF LILFLMASCS SYLVATTGEK YFKMATQAFK RGDYHKAVAF YKRSCNLRVG 70 80 90 100 110 120 VGCTSLGSMY EDGDGVDQNI TKAVFYYRRG CNLRNHLACA SLGSMYEDGD GVQKNLPKAI 130 140 150 160 170 180 YYYRRGCHLK GGVSCGSLGF MYFNGTGVKQ NYAKALFLSK YACSLNYGIS CNFVGYMYRN 190 200 210 220 230 240 AKGVQKDLKK ALANFKRGCH LKDGASCVSL GYMYEVGMDV KQNGEQALNL YKKGCYLKRG 250 260 270 280 290 300 SGCHNVAVMY YTGKGVPKDL DKAISYYKKG CTLGFSGSCK VLEEVIGKKS DDLQDDAQND TQDDMQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[2]	"Identification of a novel penicillin-binding protein from Helicobacter pylori." Krishnamurthy P., Parlow M.H., Schneider J., Burroughs S., Wickland C., Vakil N.B., Dunn B.E., Phadnis S.H. J. Bacteriol. 181:5107-5110(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PENICILLIN-BINDING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07230.1.
PIR	H64539.
RefSeq	NP_206959.1. NC_000915.1. YP_006934084.1. NC_018939.1.
3D structure databases
ProteinModelPortal	O24968.
ModBase	Search...
Protein-protein interaction databases
STRING	85962.HP0160.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07230; AAD07230; HP_0160.
GeneID	13869340. 900146.
KEGG	heo:C694_00800. hpy:HP0160.
PATRIC	20591541. VBIHelPyl33062_0170.
Phylogenomic databases
eggNOG	COG0790.
KO	K07126.
OMA	ISYYKKG.
ProtClustDB	CLSK495893.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR006597. Sel1-like. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF08238. Sel1. 7 hits. [Graphical view]
SMART	SM00671. SEL1. 7 hits. [Graphical view]
PROSITE	PS51257. PROKAR_LIPOPROTEIN. 1 hit. Uncertain. PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HCPD_HELPY

Accession

Primary (citable) accession number: O24968

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program