Reviewed, UniProtKB/Swiss-Prot O24854 (RISB_HELPY)

Names and origin

Protein names

Recommended name:
    6,7-dimethyl-8-ribityllumazine synthase
      Short name=DMRL synthase
      Short name=Lumazine synthase
    EC=2.5.1.9
Alternative name(s):
    Riboflavin synthase beta chain

Gene names

Name:	ribH
Synonyms:	ribE
Ordered Locus Names:	HP_0002

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil and riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine: step 1/1. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_0000134763

Sequences

	Sequence		Length	Mass (Da)
	O24854-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: CCC8674EA74AFAFB	FASTA	156	17,043
	10 20 30 40 50 60 MQIIEGKLQL QGNERVAILT SRFNHIITDR LQEGAMDCFK RHGGDEDLLD IVLVPGAYEL 70 80 90 100 110 120 PFILDKLLES EKYDGVCVLG AIIRGGTPHF DYVSAEATKG IAHAMLKYSM PVSFGVLTTD 130 140 150 NIEQAIERAG SKAGNKGFEA MSTLIELLSL CQTLKG

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD07075.1.
PIR	B64520.
RefSeq	NP_206804.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3051N.
Genome annotation databases
GeneID	898768.
GenomeReviews	Gene locus HP_0002 in contig AE000511_GR.
KEGG	hpy:HP0002.
NMPDR	fig|85962.1.peg.2.
TIGR	HP_0002.
Phylogenomic databases
HOGENOM	O24854.
OMA	NRAGIKA
Enzyme and pathway databases
BRENDA	2.5.1.9. 1131.
Family and domain databases
HAMAP	MF_00178. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00114. lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_HELPY

Accession

Primary (citable) accession number: O24854

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 1, 1998
Last modified:	November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)