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Protein
Submitted name:

Fatty acid alpha-hydroxylase

Gene
N/A
Organism
Sphingomonas paucimobilis (Pseudomonas paucimobilis)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi361 – 3611Iron (heme axial ligand)Combined sources

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

HemeCombined sources, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Submitted name:
Fatty acid alpha-hydroxylaseImported
OrganismiSphingomonas paucimobilis (Pseudomonas paucimobilis)Imported
Taxonomic identifieri13689 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AWMX-ray1.65A1-415[»]
3AWPX-ray1.80A1-415[»]
3AWQX-ray1.90A1-415[»]
3VM4X-ray1.94A9-415[»]
3VNOX-ray2.17A9-415[»]
3VOOX-ray2.34A9-415[»]
3VTJX-ray2.56A1-415[»]
ProteinModelPortaliO24782.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO24782.

Family & Domainsi

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

O24782-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKTPHTKGP DETLSLLADP YRFISRQCQR LGANAFESRF LLKKTNCLKG
60 70 80 90 100
AKAAEIFYDT TRFEREGAMP VAIQKTLLGQ GGVQGLDGET HRHRKQMFMG
110 120 130 140 150
LMTPERVRAL AQLFEAEWRR AVPGWTRKGE IVFYDELHEP LTRAVCAWAG
160 170 180 190 200
VPLPDDEAGN RAGELRALFD AAGSASPRHL WSRLARRRVD AWAKRIIEGI
210 220 230 240 250
RAGSIGSGSG TAAYAIAWHR DRHDDLLSPH VAAVELVNVL RPTVAIAVYI
260 270 280 290 300
TFVAHALQTC SGIRAALVQQ PDYAELFVQE VRRFYPFFPA VVARASQDFE
310 320 330 340 350
WEGMAFPEGR QVVLDLYGSN HDAATWADPQ EFRPERFRAW DEDSFNFIPQ
360 370 380 390 400
GGGDHYLGHR CPGEWIVLAI MKVAAHLLVN AMRYDVPDQD LSIDFARLPA
410
LPKSGFVMRN VHIGG
Length:415
Mass (Da):46,486
Last modified:January 1, 1998 - v1
Checksum:i3F0A1D488EF5A71E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006957 Genomic DNA. Translation: BAA22987.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006957 Genomic DNA. Translation: BAA22987.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AWMX-ray1.65A1-415[»]
3AWPX-ray1.80A1-415[»]
3AWQX-ray1.90A1-415[»]
3VM4X-ray1.94A9-415[»]
3VNOX-ray2.17A9-415[»]
3VOOX-ray2.34A9-415[»]
3VTJX-ray2.56A1-415[»]
ProteinModelPortaliO24782.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO24782.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
SUPFAMiSSF48264. SSF48264. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and expression of fatty acid alpha-hydroxylase from Sphingomonas paucimobilis."
    Matsunaga I., Yokotani N., Gotoh O., Kusunose E., Yamada M., Ichihara K.
    J. Biol. Chem. 272:23592-23596(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: EY2395Imported.
  2. "Crystal structure of H2O2-dependent cytochrome P450SPalpha with its bound fatty acid substrate: insight into the regioselective hydroxylation of fatty acids at the alpha position."
    Fujishiro T., Shoji O., Nagano S., Sugimoto H., Shiro Y., Watanabe Y.
    J. Biol. Chem. 286:29941-29950(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) IN COMPLEX WITH HEME.
  3. "Chiral-substrate-assisted stereoselective epoxidation catalyzed by H2O2-dependent cytochrome P450SPalpha."
    Fujishiro T., Shoji O., Kawakami N., Watanabe T., Sugimoto H., Shiro Y., Watanabe Y.
    Chem Asian J 7:2286-2293(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS) OF 9-415 IN COMPLEX WITH HEME.
  4. "Crystal Structure of Hydrogen Peroxide-Dependent Cytochrome P450SP alpha mutants."
    Fujishiro T., Shoji O., Sugimoto H., Shiro Y., Watanabe Y.
    Submitted (JAN-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.34 ANGSTROMS) OF 9-415 IN COMPLEX WITH HEME.
  5. "Crystal Structure of Hydrogen Peroxide-Dependent Cytochrome P450SP alpha mutants."
    Fujishiro T., Shoji O., Sugimoto H., Shiro Y., Watanabe Y.
    Submitted (MAY-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS) IN COMPLEX WITH HEME.

Entry informationi

Entry nameiO24782_SPHPI
AccessioniPrimary (citable) accession number: O24782
Entry historyi
Integrated into UniProtKB/TrEMBL: January 1, 1998
Last sequence update: January 1, 1998
Last modified: June 24, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.