O24782 (O24782_PSEPA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Fatty acid alpha-hydroxylase EMBL BAA22987.1
Organism	Pseudomonas paucimobilis (Sphingomonas paucimobilis) EMBL BAA22987.1
Taxonomic identifier	13689 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingomonas

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	Heme PDB 3AWM PDB 3AWP PDB 3AWQ Iron Metal-binding
Technical term	3D-structure PDB 3AWM PDB 3AWP PDB 3AWQ PDB 3VM4 PDB 3VNO PDB 3VOO
Gene Ontology (GO)
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	361	1	Iron (heme axial ligand) PDB 3AWM PDB 3AWP PDB 3AWQ

Sequences

	Sequence		Length	Mass (Da)
	O24782 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 3F0A1D488EF5A71E	FASTA	415	46,486
	10 20 30 40 50 60 MPKTPHTKGP DETLSLLADP YRFISRQCQR LGANAFESRF LLKKTNCLKG AKAAEIFYDT 70 80 90 100 110 120 TRFEREGAMP VAIQKTLLGQ GGVQGLDGET HRHRKQMFMG LMTPERVRAL AQLFEAEWRR 130 140 150 160 170 180 AVPGWTRKGE IVFYDELHEP LTRAVCAWAG VPLPDDEAGN RAGELRALFD AAGSASPRHL 190 200 210 220 230 240 WSRLARRRVD AWAKRIIEGI RAGSIGSGSG TAAYAIAWHR DRHDDLLSPH VAAVELVNVL 250 260 270 280 290 300 RPTVAIAVYI TFVAHALQTC SGIRAALVQQ PDYAELFVQE VRRFYPFFPA VVARASQDFE 310 320 330 340 350 360 WEGMAFPEGR QVVLDLYGSN HDAATWADPQ EFRPERFRAW DEDSFNFIPQ GGGDHYLGHR 370 380 390 400 410 CPGEWIVLAI MKVAAHLLVN AMRYDVPDQD LSIDFARLPA LPKSGFVMRN VHIGG

References

[1]	"Molecular cloning and expression of fatty acid alpha-hydroxylase from Sphingomonas paucimobilis." Matsunaga I., Yokotani N., Gotoh O., Kusunose E., Yamada M., Ichihara K. J. Biol. Chem. 272:23592-23596(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: EY2395 EMBL BAA22987.1.
[2]	"Crystal structure of H2O2-Dependent cytochrome P450SP alpha with its bound fatty acid substrate: Insight into the regioselective hydroxylation of fatty acids at the alpha position." Fujishiro T., Shoji O., Nagano S., Sugimoto H., Shiro Y., Watanabe Y. Submitted (MAR-2011) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH HEME.
[3]	"Crystal Structure of Hydrogen Peroxide-Dependent Cytochrome P450SP alpha mutants." Fujishiro T., Shoji O., Sugimoto H., Shiro Y., Watanabe Y. Submitted (JAN-2012) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.34 ANGSTROMS) OF 9-415.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB006957 Genomic DNA. Translation: BAA22987.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3AWM	X-ray	1.65	A	1-415	[»]
3AWP	X-ray	1.80	A	1-415	[»]
3AWQ	X-ray	1.90	A	1-415	[»]
3VM4	X-ray	1.94	A	9-415	[»]
3VNO	X-ray	2.17	A	9-415	[»]
3VOO	X-ray	2.34	A	9-415	[»]

ProteinModelPortal

O24782.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.630.10. 1 hit.

InterPro

IPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]

Pfam

PF00067. p450. 1 hit.
[Graphical view]

PRINTS

PR00463. EP450I.

SUPFAM

SSF48264. Cytochrome_P450. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

O24782.

Entry information

Entry name

O24782_PSEPA

Accession

Primary (citable) accession number: O24782

Entry history

Integrated into UniProtKB/TrEMBL:	January 1, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)