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O24782

- O24782_SPHPI

UniProt

O24782 - O24782_SPHPI

Protein
Submitted name:

Fatty acid alpha-hydroxylase

Gene
N/A
Organism
Sphingomonas paucimobilis (Pseudomonas paucimobilis)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi361 – 3611Iron (heme axial ligand)Imported

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Source: InterPro

    Keywords - Ligandi

    HemeImported, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Fatty acid alpha-hydroxylaseImported
    OrganismiSphingomonas paucimobilis (Pseudomonas paucimobilis)Imported
    Taxonomic identifieri13689 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3AWMX-ray1.65A1-415[»]
    3AWPX-ray1.80A1-415[»]
    3AWQX-ray1.90A1-415[»]
    3VM4X-ray1.94A9-415[»]
    3VNOX-ray2.17A9-415[»]
    3VOOX-ray2.34A9-415[»]
    3VTJX-ray2.56A1-415[»]
    ProteinModelPortaliO24782.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO24782.

    Family & Domainsi

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    SUPFAMiSSF48264. SSF48264. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O24782-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPKTPHTKGP DETLSLLADP YRFISRQCQR LGANAFESRF LLKKTNCLKG    50
    AKAAEIFYDT TRFEREGAMP VAIQKTLLGQ GGVQGLDGET HRHRKQMFMG 100
    LMTPERVRAL AQLFEAEWRR AVPGWTRKGE IVFYDELHEP LTRAVCAWAG 150
    VPLPDDEAGN RAGELRALFD AAGSASPRHL WSRLARRRVD AWAKRIIEGI 200
    RAGSIGSGSG TAAYAIAWHR DRHDDLLSPH VAAVELVNVL RPTVAIAVYI 250
    TFVAHALQTC SGIRAALVQQ PDYAELFVQE VRRFYPFFPA VVARASQDFE 300
    WEGMAFPEGR QVVLDLYGSN HDAATWADPQ EFRPERFRAW DEDSFNFIPQ 350
    GGGDHYLGHR CPGEWIVLAI MKVAAHLLVN AMRYDVPDQD LSIDFARLPA 400
    LPKSGFVMRN VHIGG 415
    Length:415
    Mass (Da):46,486
    Last modified:January 1, 1998 - v1
    Checksum:i3F0A1D488EF5A71E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006957 Genomic DNA. Translation: BAA22987.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006957 Genomic DNA. Translation: BAA22987.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3AWM X-ray 1.65 A 1-415 [» ]
    3AWP X-ray 1.80 A 1-415 [» ]
    3AWQ X-ray 1.90 A 1-415 [» ]
    3VM4 X-ray 1.94 A 9-415 [» ]
    3VNO X-ray 2.17 A 9-415 [» ]
    3VOO X-ray 2.34 A 9-415 [» ]
    3VTJ X-ray 2.56 A 1-415 [» ]
    ProteinModelPortali O24782.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei O24782.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of fatty acid alpha-hydroxylase from Sphingomonas paucimobilis."
      Matsunaga I., Yokotani N., Gotoh O., Kusunose E., Yamada M., Ichihara K.
      J. Biol. Chem. 272:23592-23596(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: EY2395Imported.
    2. "Crystal structure of H2O2-dependent cytochrome P450SPalpha with its bound fatty acid substrate: insight into the regioselective hydroxylation of fatty acids at the alpha position."
      Fujishiro T., Shoji O., Nagano S., Sugimoto H., Shiro Y., Watanabe Y.
      J. Biol. Chem. 286:29941-29950(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) IN COMPLEX WITH HEME.
    3. "Chiral-substrate-assisted stereoselective epoxidation catalyzed by H2O2-dependent cytochrome P450SP?."
      Fujishiro T., Shoji O., Kawakami N., Watanabe T., Sugimoto H., Shiro Y., Watanabe Y.
      Chem Asian J 7:2286-2293(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS) OF 9-415 IN COMPLEX WITH HEME.
    4. "Crystal Structure of Hydrogen Peroxide-Dependent Cytochrome P450SP alpha mutants."
      Fujishiro T., Shoji O., Sugimoto H., Shiro Y., Watanabe Y.
      Submitted (JAN-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.34 ANGSTROMS) OF 9-415 IN COMPLEX WITH HEME.
    5. "Crystal Structure of Hydrogen Peroxide-Dependent Cytochrome P450SP alpha mutants."
      Fujishiro T., Shoji O., Sugimoto H., Shiro Y., Watanabe Y.
      Submitted (MAY-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS) IN COMPLEX WITH HEME.

    Entry informationi

    Entry nameiO24782_SPHPI
    AccessioniPrimary (citable) accession number: O24782
    Entry historyi
    Integrated into UniProtKB/TrEMBL: January 1, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3