Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

AS

Organism
Triphysaria versicolor (Yellow owl's clover)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Asparagine synthetase [glutamine-hydrolyzing] (AS)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei98GlutamineBy similarity1
Binding sitei231ATP; via carbonyl oxygenBy similarity1
Binding sitei267ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei343Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi341 – 342ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:AS
OrganismiTriphysaria versicolor (Yellow owl's clover)
Taxonomic identifieri64093 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesOrobanchaceaePedicularideaeCastillejinaeTriphysaria

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569292 – 586Asparagine synthetase [glutamine-hydrolyzing]Add BLAST585

Structurei

3D structure databases

ProteinModelPortaliO24661.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST184
Domaini193 – 516Asparagine synthetaseAdd BLAST324

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 54Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24661-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILAVLGC SDDSQAKRVR VLELSRRLKH RGPDWSGIHH HGDCYLAHQR
60 70 80 90 100
LAIVDPASGD QPLFNEDKRI AVTVNGEIYN HEELRALLPN HKFRTGSDCD
110 120 130 140 150
VIAHLYEEYG ENFVEMLDGM FSFVLLDSRD NTFIAARDAF GITSLYIGWG
160 170 180 190 200
LDGSVWISSE LKGLHDECEN FEVFPPGHVY SSKTEGFRRW YNPPWFSEAI
210 220 230 240 250
PSTPYDPLVL RGAFEQAVIK RLMTDVPFGV LLSGGLDSSL VAAVTARHLA
260 270 280 290 300
GTKAAKRWGS QLHSFCVGLE GSPDLKAGKE VADYLGTVHH EFLFTVQDGI
310 320 330 340 350
DAIEDVIYHI ETYDVTTIRA STPMFLMSRK IKSLGVKMVI SGEGSDEIFG
360 370 380 390 400
GYLYFHKAPN KEEFHRETCR KIKALHQYDC LRANKATSAW GLEARVPFLD
410 420 430 440 450
KEFVNLAMSI DPEAKMIKPD QGRIEKWILR KAFDDEERPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYS WIDGLKAHAE QHVTDKMMLN AGHIFPHNTP TTKEGYYYRM
510 520 530 540 550
IFERFFPQNS AKLTVPGGPS VACSTATAVA WDASWSKNLD PSGRAATGVH
560 570 580
DLAYENHVPI GNLKSKKMDS VSLGNAVGPQ ELTIRS
Length:586
Mass (Da):65,692
Last modified:January 23, 2007 - v3
Checksum:i81D923F9D6BDCCE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014055 mRNA. Translation: AAD05033.1.
AF014056 mRNA. Translation: AAD05034.1.
AF014057 mRNA. Translation: AAD05035.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014055 mRNA. Translation: AAD05033.1.
AF014056 mRNA. Translation: AAD05034.1.
AF014057 mRNA. Translation: AAD05035.1.

3D structure databases

ProteinModelPortaliO24661.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASNS_TRIVS
AccessioniPrimary (citable) accession number: O24661
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.