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O24661

- ASNS_TRIVS

UniProt

O24661 - ASNS_TRIVS

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Protein
Asparagine synthetase [glutamine-hydrolyzing]
Gene
AS
Organism
Triphysaria versicolor (Yellow owl's clover)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity
Binding sitei98 – 981Glutamine By similarity
Binding sitei231 – 2311ATP; via carbonyl oxygen By similarity
Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygen By similarity
Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formation By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi341 – 3422ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:AS
OrganismiTriphysaria versicolor (Yellow owl's clover)
Taxonomic identifieri64093 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesOrobanchaceaePedicularideaeCastillejinaeTriphysaria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 586585Asparagine synthetase [glutamine-hydrolyzing]
PRO_0000056929Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO24661.
SMRiO24661. Positions 1-517.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 185184Glutamine amidotransferase type-2
Add
BLAST
Domaini193 – 516324Asparagine synthetase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine binding By similarity
Regioni75 – 773Glutamine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24661-1 [UniParc]FASTAAdd to Basket

« Hide

MCGILAVLGC SDDSQAKRVR VLELSRRLKH RGPDWSGIHH HGDCYLAHQR    50
LAIVDPASGD QPLFNEDKRI AVTVNGEIYN HEELRALLPN HKFRTGSDCD 100
VIAHLYEEYG ENFVEMLDGM FSFVLLDSRD NTFIAARDAF GITSLYIGWG 150
LDGSVWISSE LKGLHDECEN FEVFPPGHVY SSKTEGFRRW YNPPWFSEAI 200
PSTPYDPLVL RGAFEQAVIK RLMTDVPFGV LLSGGLDSSL VAAVTARHLA 250
GTKAAKRWGS QLHSFCVGLE GSPDLKAGKE VADYLGTVHH EFLFTVQDGI 300
DAIEDVIYHI ETYDVTTIRA STPMFLMSRK IKSLGVKMVI SGEGSDEIFG 350
GYLYFHKAPN KEEFHRETCR KIKALHQYDC LRANKATSAW GLEARVPFLD 400
KEFVNLAMSI DPEAKMIKPD QGRIEKWILR KAFDDEERPY LPKHILYRQK 450
EQFSDGVGYS WIDGLKAHAE QHVTDKMMLN AGHIFPHNTP TTKEGYYYRM 500
IFERFFPQNS AKLTVPGGPS VACSTATAVA WDASWSKNLD PSGRAATGVH 550
DLAYENHVPI GNLKSKKMDS VSLGNAVGPQ ELTIRS 586
Length:586
Mass (Da):65,692
Last modified:January 23, 2007 - v3
Checksum:i81D923F9D6BDCCE9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF014055 mRNA. Translation: AAD05033.1.
AF014056 mRNA. Translation: AAD05034.1.
AF014057 mRNA. Translation: AAD05035.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF014055 mRNA. Translation: AAD05033.1 .
AF014056 mRNA. Translation: AAD05034.1 .
AF014057 mRNA. Translation: AAD05035.1 .

3D structure databases

ProteinModelPortali O24661.
SMRi O24661. Positions 1-517.
ModBasei Search...

Protein family/group databases

MEROPSi C44.976.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Host-root exudates increase gene expression of asparagine synthetase in the roots of a hemiparasitic plant Triphysaria versicolor (Scrophulariaceae)."
    Delavault P., Estabrook E., Albrecht H., Wrobel R., Yoder J.I.
    Gene 222:155-162(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Root.

Entry informationi

Entry nameiASNS_TRIVS
AccessioniPrimary (citable) accession number: O24661
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 76 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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