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O24661

- ASNS_TRIVS

UniProt

O24661 - ASNS_TRIVS

Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

AS

Organism
Triphysaria versicolor (Yellow owl's clover)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei98 – 981GlutamineBy similarity
    Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
    Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi341 – 3422ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00134; UER00195.

    Protein family/group databases

    MEROPSiC44.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase
    Gene namesi
    Name:AS
    OrganismiTriphysaria versicolor (Yellow owl's clover)
    Taxonomic identifieri64093 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesOrobanchaceaePedicularideaeCastillejinaeTriphysaria

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 586585Asparagine synthetase [glutamine-hydrolyzing]PRO_0000056929Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO24661.
    SMRiO24661. Positions 1-517.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini193 – 516324Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 545Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O24661-1 [UniParc]FASTAAdd to Basket

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    MCGILAVLGC SDDSQAKRVR VLELSRRLKH RGPDWSGIHH HGDCYLAHQR    50
    LAIVDPASGD QPLFNEDKRI AVTVNGEIYN HEELRALLPN HKFRTGSDCD 100
    VIAHLYEEYG ENFVEMLDGM FSFVLLDSRD NTFIAARDAF GITSLYIGWG 150
    LDGSVWISSE LKGLHDECEN FEVFPPGHVY SSKTEGFRRW YNPPWFSEAI 200
    PSTPYDPLVL RGAFEQAVIK RLMTDVPFGV LLSGGLDSSL VAAVTARHLA 250
    GTKAAKRWGS QLHSFCVGLE GSPDLKAGKE VADYLGTVHH EFLFTVQDGI 300
    DAIEDVIYHI ETYDVTTIRA STPMFLMSRK IKSLGVKMVI SGEGSDEIFG 350
    GYLYFHKAPN KEEFHRETCR KIKALHQYDC LRANKATSAW GLEARVPFLD 400
    KEFVNLAMSI DPEAKMIKPD QGRIEKWILR KAFDDEERPY LPKHILYRQK 450
    EQFSDGVGYS WIDGLKAHAE QHVTDKMMLN AGHIFPHNTP TTKEGYYYRM 500
    IFERFFPQNS AKLTVPGGPS VACSTATAVA WDASWSKNLD PSGRAATGVH 550
    DLAYENHVPI GNLKSKKMDS VSLGNAVGPQ ELTIRS 586
    Length:586
    Mass (Da):65,692
    Last modified:January 23, 2007 - v3
    Checksum:i81D923F9D6BDCCE9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF014055 mRNA. Translation: AAD05033.1.
    AF014056 mRNA. Translation: AAD05034.1.
    AF014057 mRNA. Translation: AAD05035.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF014055 mRNA. Translation: AAD05033.1 .
    AF014056 mRNA. Translation: AAD05034.1 .
    AF014057 mRNA. Translation: AAD05035.1 .

    3D structure databases

    ProteinModelPortali O24661.
    SMRi O24661. Positions 1-517.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.976.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Host-root exudates increase gene expression of asparagine synthetase in the roots of a hemiparasitic plant Triphysaria versicolor (Scrophulariaceae)."
      Delavault P., Estabrook E., Albrecht H., Wrobel R., Yoder J.I.
      Gene 222:155-162(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Root.

    Entry informationi

    Entry nameiASNS_TRIVS
    AccessioniPrimary (citable) accession number: O24661
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 77 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3