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Protein

Gibberellin 3-beta-dioxygenase 1

Gene

LE

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts the inactive gibbberellin (GA) precursors GA9 and GA20 in the bioactives gibberellins GA4 and GA1. Has a small activity on GA29, producing GA8. Unable to convert GA20 to GA5, GA5 to GA3 or GA12 to GA14. Involved in the production of bioactive GA for vegetative growth and development, but not for the 3-beta-hydroxylation of GA in developing seeds.3 Publications

Catalytic activityi

Gibberellin 20 + 2-oxoglutarate + O2 = gibberellin 1 + succinate + CO2.3 Publications

Cofactori

Protein has several cofactor binding sites:
  • L-ascorbateBy similarity
  • Fe2+PROSITE-ProRule annotationNote: Binds 1 Fe2+ ion per subunit.PROSITE-ProRule annotation

Kineticsi

  1. KM=1.5 µM for GA91 Publication
  2. KM=13 µM for GA201 Publication
  1. Vmax=0.29 nmol/min/mg enzyme with GA9 as substrate1 Publication
  2. Vmax=0.10 nmol/min/mg enzyme with GA20 as substrate1 Publication

Pathwayi: gibberellin biosynthesis

This protein is involved in the pathway gibberellin biosynthesis, which is part of Plant hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gibberellin biosynthesis and in Plant hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi231IronPROSITE-ProRule annotation1
Metal bindingi233IronPROSITE-ProRule annotation1
Metal bindingi288IronPROSITE-ProRule annotation1
Active sitei298Sequence analysis1
Binding sitei2982-oxoglutaratePROSITE-ProRule annotation1

GO - Molecular functioni

  • gibberellin 3-beta-dioxygenase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • gibberellin biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00390.

Names & Taxonomyi

Protein namesi
Recommended name:
Gibberellin 3-beta-dioxygenase 1 (EC:1.14.11.15)
Alternative name(s):
GA 3-oxidase 1
Gibberellin 3 beta-hydroxylase 1
Gene namesi
Name:LE
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Pathology & Biotechi

Disruption phenotypei

Dwarf phenotype.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi89L → F: Strongly decreased catalytic activity producing a dwarf phenotype; when associated with Y-276. 1 Publication1
Mutagenesisi229A → T in le-1; strongly decreased catalytic activity producing a dwarf phenotype. 2 Publications1
Mutagenesisi276H → Y in le-3; strongly decreased catalytic activity producing a dwarf phenotype. Strongly decreased catalytic activity producing a dwarf phenotype; when associated with F-89. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004252391 – 374Gibberellin 3-beta-dioxygenase 1Add BLAST374

Expressioni

Tissue specificityi

Expressed in radicles, roots, internodes, cotyledons, leaves and shoots. Barely detected in developing seeds. Not detected in flowers or young fruits.2 Publications

Developmental stagei

Highly expressed in germinating seedlings.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO24648.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini206 – 307Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST102

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O24648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSLSEAYRA HPVHVNHKHP DFNSLQELPE SYNWTHLDDH TLIDSNNIMK
60 70 80 90 100
ESTTTVPVID LNDPNASKLI GLACKTWGVY QVMNHGIPLS LLEDIQWLGQ
110 120 130 140 150
TLFSLPSHQK HKATRSPDGV SGYGIARISS FFPKLMWYEG FTIVGSPLDH
160 170 180 190 200
FRELWPQDYT RFCDIVVQYD ETMKKLAGTL MCLMLDSLGI TKEDIKWAGS
210 220 230 240 250
KAQFEKACAA LQLNSYPSCP DPDHAMGLAP HTDSTFLTIL SQNDISGLQV
260 270 280 290 300
NREGSGWITV PPLQGGLVVN VGDLFHILSN GLYPSVLHRV LVNRTRQRFS
310 320 330 340 350
VAYLYGPPSN VEICPHAKLI GPTKPPLYRS VTWNEYLGTK AKHFNKALSS
360 370
VRLCTPINGL FDVNDSNKNS VQVG
Length:374
Mass (Da):41,726
Last modified:January 1, 1998 - v1
Checksum:iB7203F5FB264C9C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85045 mRNA. Translation: AAB65829.1.
AF001219 mRNA. Translation: AAC49792.1.
AF010167 mRNA. Translation: AAC49793.1.
AF010168 mRNA. Translation: AAC49794.1.
U93210 Genomic DNA. Translation: AAC86820.1.
AF007766 mRNA. Translation: AAC96017.1.
AF004730 Genomic DNA. Translation: AAC96015.1.
PIRiT06244.
T06245.

Cross-referencesi

Web resourcesi

Protein Spotlight

On the garden pea - Issue 159 of April 2014

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85045 mRNA. Translation: AAB65829.1.
AF001219 mRNA. Translation: AAC49792.1.
AF010167 mRNA. Translation: AAC49793.1.
AF010168 mRNA. Translation: AAC49794.1.
U93210 Genomic DNA. Translation: AAC86820.1.
AF007766 mRNA. Translation: AAC96017.1.
AF004730 Genomic DNA. Translation: AAC96015.1.
PIRiT06244.
T06245.

3D structure databases

ProteinModelPortaliO24648.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00390.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3OX_PEA
AccessioniPrimary (citable) accession number: O24648
Secondary accession number(s): O22377, O24623, O24627
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The Le gene controls the stem length trait studied by Gregor Mendel in 1866 (PubMed:9286112 and PubMed:9238076).

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.