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O24648

- G3OX_PEA

UniProt

O24648 - G3OX_PEA

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Protein

Gibberellin 3-beta-dioxygenase 1

Gene

LE

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Converts the inactive gibbberellin (GA) precursors GA9 and GA20 in the bioactives gibberellins GA4 and GA1. Has a small activity on GA29, producing GA8. Unable to convert GA20 to GA5, GA5 to GA3 or GA12 to GA14. Involved in the production of bioactive GA for vegetative growth and development, but not for the 3-beta-hydroxylation of GA in developing seeds.3 Publications

Catalytic activityi

Gibberellin 20 + 2-oxoglutarate + O2 = gibberellin 1 + succinate + CO2.3 Publications

Cofactori

Protein has several cofactor binding sites:
  • L-ascorbateBy similarity
  • Fe2+PROSITE-ProRule annotationNote: Binds 1 Fe(2+) ion per subunit.PROSITE-ProRule annotation

Kineticsi

  1. KM=1.5 µM for GA91 Publication
  2. KM=13 µM for GA201 Publication

Vmax=0.29 nmol/min/mg enzyme with GA9 as substrate1 Publication

Vmax=0.10 nmol/min/mg enzyme with GA20 as substrate1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi231 – 2311IronPROSITE-ProRule annotation
Metal bindingi233 – 2331IronPROSITE-ProRule annotation
Metal bindingi288 – 2881IronPROSITE-ProRule annotation
Active sitei298 – 2981Sequence Analysis
Binding sitei298 – 29812-oxoglutaratePROSITE-ProRule annotation

GO - Molecular functioni

  1. gibberellin 3-beta-dioxygenase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. gibberellin biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00390.

Names & Taxonomyi

Protein namesi
Recommended name:
Gibberellin 3-beta-dioxygenase 1 (EC:1.14.11.15)
Alternative name(s):
GA 3-oxidase 1
Gibberellin 3 beta-hydroxylase 1
Gene namesi
Name:LE
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Pathology & Biotechi

Disruption phenotypei

Dwarf phenotype.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi89 – 891L → F: Strongly decreased catalytic activity producing a dwarf phenotype; when associated with Y-276. 1 Publication
Mutagenesisi229 – 2291A → T in le-1; strongly decreased catalytic activity producing a dwarf phenotype. 2 Publications
Mutagenesisi276 – 2761H → Y in le-3; strongly decreased catalytic activity producing a dwarf phenotype. Strongly decreased catalytic activity producing a dwarf phenotype; when associated with F-89. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 374374Gibberellin 3-beta-dioxygenase 1PRO_0000425239Add
BLAST

Expressioni

Tissue specificityi

Expressed in radicles, roots, internodes, cotyledons, leaves and shoots. Barely detected in developing seeds. Not detected in flowers or young fruits.2 Publications

Developmental stagei

Highly expressed in germinating seedlings.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO24648.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini206 – 307102Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O24648-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSLSEAYRA HPVHVNHKHP DFNSLQELPE SYNWTHLDDH TLIDSNNIMK
60 70 80 90 100
ESTTTVPVID LNDPNASKLI GLACKTWGVY QVMNHGIPLS LLEDIQWLGQ
110 120 130 140 150
TLFSLPSHQK HKATRSPDGV SGYGIARISS FFPKLMWYEG FTIVGSPLDH
160 170 180 190 200
FRELWPQDYT RFCDIVVQYD ETMKKLAGTL MCLMLDSLGI TKEDIKWAGS
210 220 230 240 250
KAQFEKACAA LQLNSYPSCP DPDHAMGLAP HTDSTFLTIL SQNDISGLQV
260 270 280 290 300
NREGSGWITV PPLQGGLVVN VGDLFHILSN GLYPSVLHRV LVNRTRQRFS
310 320 330 340 350
VAYLYGPPSN VEICPHAKLI GPTKPPLYRS VTWNEYLGTK AKHFNKALSS
360 370
VRLCTPINGL FDVNDSNKNS VQVG
Length:374
Mass (Da):41,726
Last modified:January 1, 1998 - v1
Checksum:iB7203F5FB264C9C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85045 mRNA. Translation: AAB65829.1.
AF001219 mRNA. Translation: AAC49792.1.
AF010167 mRNA. Translation: AAC49793.1.
AF010168 mRNA. Translation: AAC49794.1.
U93210 Genomic DNA. Translation: AAC86820.1.
AF007766 mRNA. Translation: AAC96017.1.
AF004730 Genomic DNA. Translation: AAC96015.1.
PIRiT06244.
T06245.

Cross-referencesi

Web resourcesi

Protein Spotlight

On the garden pea - Issue 159 of April 2014

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85045 mRNA. Translation: AAB65829.1 .
AF001219 mRNA. Translation: AAC49792.1 .
AF010167 mRNA. Translation: AAC49793.1 .
AF010168 mRNA. Translation: AAC49794.1 .
U93210 Genomic DNA. Translation: AAC86820.1 .
AF007766 mRNA. Translation: AAC96017.1 .
AF004730 Genomic DNA. Translation: AAC96015.1 .
PIRi T06244.
T06245.

3D structure databases

ProteinModelPortali O24648.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00390 .

Family and domain databases

Gene3Di 2.60.120.330. 1 hit.
InterProi IPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view ]
Pfami PF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view ]
PROSITEi PS51471. FE2OG_OXY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mendel's stem length gene (Le) encodes a gibberellin 3 beta-hydroxylase."
    Lester D.R., Ross J.J., Davies P.J., Reid J.B.
    Plant Cell 9:1435-1443(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ALA-229.
  2. "Mendel's dwarfing gene: cDNAs from the Le alleles and function of the expressed proteins."
    Martin D.N., Proebsting W.M., Hedden P.
    Proc. Natl. Acad. Sci. U.S.A. 94:8907-8911(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-89; ALA-229 AND HIS-276, DISRUPTION PHENOTYPE.
  3. "The influence of the null le-2 mutation on gibberellin levels in developing pea seeds."
    Lester D.R., Mackenzie-Hose A.K., Davies P.J., Ross J.J., Reid J.B.
    Plant Growth Regul. 27:83-89(1999)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF HIS-276, FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiG3OX_PEA
AccessioniPrimary (citable) accession number: O24648
Secondary accession number(s): O22377, O24623, O24627
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The Le gene controls the stem length trait studied by Gregor Mendel in 1866 (PubMed:9286112 and PubMed:9238076).

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3