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O24648 (G3OX_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gibberellin 3-beta-dioxygenase 1

EC=1.14.11.15
Alternative name(s):
GA 3-oxidase 1
Gibberellin 3 beta-hydroxylase 1
Gene names
Name:LE
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts the inactive gibbberellin (GA) precursors GA9 and GA20 in the bioactives gibberellins GA4 and GA1. Has a small activity on GA29, producing GA8. Unable to convert GA20 to GA5, GA5 to GA3 or GA12 to GA14. Involved in the production of bioactive GA for vegetative growth and development, but not for the 3-beta-hydroxylation of GA in developing seeds. Ref.1 Ref.2 Ref.3

Catalytic activity

Gibberellin 20 + 2-oxoglutarate + O2 = gibberellin 1 + succinate + CO2. Ref.1 Ref.2 Ref.3

Cofactor

Ascorbate By similarity.

Binds 1 Fe2+ ion per subunit By similarity.

Pathway

Plant hormone biosynthesis; gibberellin biosynthesis.

Tissue specificity

Expressed in radicles, roots, internodes, cotyledons, leaves and shoots. Barely detected in developing seeds. Not detected in flowers or young fruits. Ref.2 Ref.3

Developmental stage

Highly expressed in germinating seedlings. Ref.2

Disruption phenotype

Dwarf phenotype. Ref.2

Miscellaneous

The Le gene controls the stem length trait studied by Gregor Mendel in 1866 (Ref.1 and Ref.2).

Sequence similarities

Belongs to the iron/ascorbate-dependent oxidoreductase family. GA3OX subfamily.

Contains 1 Fe2OG dioxygenase domain.

Biophysicochemical properties

Kinetic parameters:

KM=1.5 µM for GA9 Ref.2

KM=13 µM for GA20

Vmax=0.29 nmol/min/mg enzyme with GA9 as substrate

Vmax=0.10 nmol/min/mg enzyme with GA20 as substrate

Ontologies

Keywords
   LigandIron
Metal-binding
   Molecular functionDioxygenase
Oxidoreductase
Gene Ontology (GO)
   Biological_processgibberellin biosynthetic process

Inferred from direct assay Ref.2. Source: UniProtKB

   Molecular_functiongibberellin 3-beta-dioxygenase activity

Inferred from direct assay Ref.2. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374Gibberellin 3-beta-dioxygenase 1
PRO_0000425239

Regions

Domain206 – 307102Fe2OG dioxygenase

Sites

Active site2981 Potential
Metal binding2311Iron By similarity
Metal binding2331Iron By similarity
Metal binding2881Iron By similarity
Binding site29812-oxoglutarate Potential

Experimental info

Mutagenesis891L → F: Strongly decreased catalytic activity producing a dwarf phenotype; when associated with Y-276. Ref.2
Mutagenesis2291A → T in le-1; strongly decreased catalytic activity producing a dwarf phenotype. Ref.1 Ref.2
Mutagenesis2761H → Y in le-3; strongly decreased catalytic activity producing a dwarf phenotype. Strongly decreased catalytic activity producing a dwarf phenotype; when associated with F-89. Ref.2 Ref.3

Sequences

Sequence LengthMass (Da)Tools
O24648 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: B7203F5FB264C9C9

FASTA37441,726
        10         20         30         40         50         60 
MPSLSEAYRA HPVHVNHKHP DFNSLQELPE SYNWTHLDDH TLIDSNNIMK ESTTTVPVID 

        70         80         90        100        110        120 
LNDPNASKLI GLACKTWGVY QVMNHGIPLS LLEDIQWLGQ TLFSLPSHQK HKATRSPDGV 

       130        140        150        160        170        180 
SGYGIARISS FFPKLMWYEG FTIVGSPLDH FRELWPQDYT RFCDIVVQYD ETMKKLAGTL 

       190        200        210        220        230        240 
MCLMLDSLGI TKEDIKWAGS KAQFEKACAA LQLNSYPSCP DPDHAMGLAP HTDSTFLTIL 

       250        260        270        280        290        300 
SQNDISGLQV NREGSGWITV PPLQGGLVVN VGDLFHILSN GLYPSVLHRV LVNRTRQRFS 

       310        320        330        340        350        360 
VAYLYGPPSN VEICPHAKLI GPTKPPLYRS VTWNEYLGTK AKHFNKALSS VRLCTPINGL 

       370 
FDVNDSNKNS VQVG 

« Hide

References

[1]"Mendel's stem length gene (Le) encodes a gibberellin 3 beta-hydroxylase."
Lester D.R., Ross J.J., Davies P.J., Reid J.B.
Plant Cell 9:1435-1443(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ALA-229.
[2]"Mendel's dwarfing gene: cDNAs from the Le alleles and function of the expressed proteins."
Martin D.N., Proebsting W.M., Hedden P.
Proc. Natl. Acad. Sci. U.S.A. 94:8907-8911(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-89; ALA-229 AND HIS-276, DISRUPTION PHENOTYPE.
[3]"The influence of the null le-2 mutation on gibberellin levels in developing pea seeds."
Lester D.R., Mackenzie-Hose A.K., Davies P.J., Ross J.J., Reid J.B.
Plant Growth Regul. 27:83-89(1999)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF HIS-276, FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

Web resources

Protein Spotlight

On the garden pea - Issue 159 of April 2014

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U85045 mRNA. Translation: AAB65829.1.
AF001219 mRNA. Translation: AAC49792.1.
AF010167 mRNA. Translation: AAC49793.1.
AF010168 mRNA. Translation: AAC49794.1.
U93210 Genomic DNA. Translation: AAC86820.1.
AF007766 mRNA. Translation: AAC96017.1.
AF004730 Genomic DNA. Translation: AAC96015.1.
PIRT06244.
T06245.

3D structure databases

ProteinModelPortalO24648.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00390.

Family and domain databases

Gene3D2.60.120.330. 1 hit.
InterProIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3OX_PEA
AccessionPrimary (citable) accession number: O24648
Secondary accession number(s): O22377, O24623, O24627
Entry history
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PATHWAY comments

Index of metabolic and biosynthesis pathways