Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O24648

- G3OX_PEA

UniProt

O24648 - G3OX_PEA

Protein

Gibberellin 3-beta-dioxygenase 1

Gene

LE

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Converts the inactive gibbberellin (GA) precursors GA9 and GA20 in the bioactives gibberellins GA4 and GA1. Has a small activity on GA29, producing GA8. Unable to convert GA20 to GA5, GA5 to GA3 or GA12 to GA14. Involved in the production of bioactive GA for vegetative growth and development, but not for the 3-beta-hydroxylation of GA in developing seeds.3 Publications

    Catalytic activityi

    Gibberellin 20 + 2-oxoglutarate + O2 = gibberellin 1 + succinate + CO2.3 Publications

    Cofactori

    Ascorbate.By similarity
    Binds 1 Fe2+ ion per subunit.PROSITE-ProRule annotation

    Kineticsi

    1. KM=1.5 µM for GA91 Publication
    2. KM=13 µM for GA201 Publication

    Vmax=0.29 nmol/min/mg enzyme with GA9 as substrate1 Publication

    Vmax=0.10 nmol/min/mg enzyme with GA20 as substrate1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi231 – 2311IronPROSITE-ProRule annotation
    Metal bindingi233 – 2331IronPROSITE-ProRule annotation
    Metal bindingi288 – 2881IronPROSITE-ProRule annotation
    Active sitei298 – 2981Sequence Analysis
    Binding sitei298 – 29812-oxoglutaratePROSITE-ProRule annotation

    GO - Molecular functioni

    1. gibberellin 3-beta-dioxygenase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. gibberellin biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00390.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gibberellin 3-beta-dioxygenase 1 (EC:1.14.11.15)
    Alternative name(s):
    GA 3-oxidase 1
    Gibberellin 3 beta-hydroxylase 1
    Gene namesi
    Name:LE
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    Pathology & Biotechi

    Disruption phenotypei

    Dwarf phenotype.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi89 – 891L → F: Strongly decreased catalytic activity producing a dwarf phenotype; when associated with Y-276. 1 Publication
    Mutagenesisi229 – 2291A → T in le-1; strongly decreased catalytic activity producing a dwarf phenotype. 2 Publications
    Mutagenesisi276 – 2761H → Y in le-3; strongly decreased catalytic activity producing a dwarf phenotype. Strongly decreased catalytic activity producing a dwarf phenotype; when associated with F-89. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 374374Gibberellin 3-beta-dioxygenase 1PRO_0000425239Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in radicles, roots, internodes, cotyledons, leaves and shoots. Barely detected in developing seeds. Not detected in flowers or young fruits.2 Publications

    Developmental stagei

    Highly expressed in germinating seedlings.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO24648.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini206 – 307102Fe2OG dioxygenasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.60.120.330. 1 hit.
    InterProiIPR026992. DIOX_N.
    IPR027443. IPNS-like.
    IPR005123. Oxoglu/Fe-dep_dioxygenase.
    [Graphical view]
    PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
    PF14226. DIOX_N. 1 hit.
    [Graphical view]
    PROSITEiPS51471. FE2OG_OXY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O24648-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSLSEAYRA HPVHVNHKHP DFNSLQELPE SYNWTHLDDH TLIDSNNIMK    50
    ESTTTVPVID LNDPNASKLI GLACKTWGVY QVMNHGIPLS LLEDIQWLGQ 100
    TLFSLPSHQK HKATRSPDGV SGYGIARISS FFPKLMWYEG FTIVGSPLDH 150
    FRELWPQDYT RFCDIVVQYD ETMKKLAGTL MCLMLDSLGI TKEDIKWAGS 200
    KAQFEKACAA LQLNSYPSCP DPDHAMGLAP HTDSTFLTIL SQNDISGLQV 250
    NREGSGWITV PPLQGGLVVN VGDLFHILSN GLYPSVLHRV LVNRTRQRFS 300
    VAYLYGPPSN VEICPHAKLI GPTKPPLYRS VTWNEYLGTK AKHFNKALSS 350
    VRLCTPINGL FDVNDSNKNS VQVG 374
    Length:374
    Mass (Da):41,726
    Last modified:January 1, 1998 - v1
    Checksum:iB7203F5FB264C9C9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U85045 mRNA. Translation: AAB65829.1.
    AF001219 mRNA. Translation: AAC49792.1.
    AF010167 mRNA. Translation: AAC49793.1.
    AF010168 mRNA. Translation: AAC49794.1.
    U93210 Genomic DNA. Translation: AAC86820.1.
    AF007766 mRNA. Translation: AAC96017.1.
    AF004730 Genomic DNA. Translation: AAC96015.1.
    PIRiT06244.
    T06245.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    On the garden pea - Issue 159 of April 2014

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U85045 mRNA. Translation: AAB65829.1 .
    AF001219 mRNA. Translation: AAC49792.1 .
    AF010167 mRNA. Translation: AAC49793.1 .
    AF010168 mRNA. Translation: AAC49794.1 .
    U93210 Genomic DNA. Translation: AAC86820.1 .
    AF007766 mRNA. Translation: AAC96017.1 .
    AF004730 Genomic DNA. Translation: AAC96015.1 .
    PIRi T06244.
    T06245.

    3D structure databases

    ProteinModelPortali O24648.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00390 .

    Family and domain databases

    Gene3Di 2.60.120.330. 1 hit.
    InterProi IPR026992. DIOX_N.
    IPR027443. IPNS-like.
    IPR005123. Oxoglu/Fe-dep_dioxygenase.
    [Graphical view ]
    Pfami PF03171. 2OG-FeII_Oxy. 1 hit.
    PF14226. DIOX_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51471. FE2OG_OXY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mendel's stem length gene (Le) encodes a gibberellin 3 beta-hydroxylase."
      Lester D.R., Ross J.J., Davies P.J., Reid J.B.
      Plant Cell 9:1435-1443(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ALA-229.
    2. "Mendel's dwarfing gene: cDNAs from the Le alleles and function of the expressed proteins."
      Martin D.N., Proebsting W.M., Hedden P.
      Proc. Natl. Acad. Sci. U.S.A. 94:8907-8911(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-89; ALA-229 AND HIS-276, DISRUPTION PHENOTYPE.
    3. "The influence of the null le-2 mutation on gibberellin levels in developing pea seeds."
      Lester D.R., Mackenzie-Hose A.K., Davies P.J., Ross J.J., Reid J.B.
      Plant Growth Regul. 27:83-89(1999)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF HIS-276, FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiG3OX_PEA
    AccessioniPrimary (citable) accession number: O24648
    Secondary accession number(s): O22377, O24623, O24627
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 19, 2014
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The Le gene controls the stem length trait studied by Gregor Mendel in 1866 (PubMed:9286112 and PubMed:9238076).

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3