Reviewed, UniProtKB/Swiss-Prot O24646 (HY5_ARATH)

Names and origin

Protein names

Recommended name:
    Transcription factor HY5
Alternative name(s):
    Protein LONG HYPOCOTYL 5
    bZIP transcription factor 56
      Short name=AtbZIP56

Gene names

Name:	HY5
Synonyms:	BZIP56
Ordered Locus Names:	At5g11260
ORF Names:	F2I11_150

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	168 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcription factor that promotes photomorphogenesis in the light. Acts downstream of the light receptor network and directly affects transcription of light-induced genes. Specifically involved in blue-light specific pathway, suggesting that it participates in transmission of cryptochromes (CRY1 and CRY2) signals to downstream responses. In darkness, its degradation prevent the activation of light-induced genes.
Subunit structure	Heterodimer; heterodimerizes with HYH via the leucine-zipper domains. Interacts with COP1 WD40 domain. Ref.5 Ref.8
Subcellular location	Nucleus.
Tissue specificity	Expressed in root, hypocotyl, cotyledon, leaf, stem and floral organs.
Post-translational modification	Phosphorylated by CK2. Shows a stronger interaction with COP1 when unphosphorylated. However, phosphorylation does not affect its susceptibility to be ubiquitinated. Ref.4 Ref.9 Ref.10 Ubiquitinated by COP1 and/or CIP8. Ubiquitination takes place in darkness and leads to its subsequent degradation, thereby preventing to activate photomorphogenesis signals. Ref.3 Ref.6
Sequence similarities	Belongs to the bZIP family. Contains 1 bZIP domain.

Ontologies

Keywords
Biological process	Phytochrome signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	gibberellic acid mediated signaling pathway Inferred from mutant phenotype. Source: TAIR positive regulation of anthocyanin metabolic process Inferred from mutant phenotype. Source: TAIR red, far-red light phototransduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of photomorphogenesis Inferred from mutant phenotype. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to UV-B Inferred from mutant phenotype. Source: TAIR response to abscisic acid stimulus Inferred from mutant phenotype. Source: TAIR response to far red light Inferred from expression pattern. Source: TAIR response to red light Inferred from expression pattern. Source: TAIR transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: TAIR
Molecular function	double-stranded DNA binding Inferred from physical interaction. Source: TAIR protein dimerization activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
At1g75540	Q9LQZ7	1	EBI-301660,EBI-1994459
At1g78600	Q9SYM2	1	EBI-301660,EBI-1994217
CIP8	Q9SPL2	1	EBI-301660,EBI-301644
COP1	P43254	6	EBI-301660,EBI-301649
HYH	Q8W191	1	EBI-301660,EBI-631836

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 168

168

Transcription factor HY5

PRO_0000076560

Regions

Domain

116 – 144

29

Leucine-zipper

DNA binding

93 – 110

18

Basic motif

Region

35 – 46

12

Interaction with COP1

Amino acid modifications

Modified residue

36

1

Phosphoserine Ref.9 Ref.10

Experimental info

Mutagenesis

43 – 44

2

VP → AA: Abolishes interaction with COP1. Ref.5

Secondary structure

1

.

.

.

168

Helix Strand Turn

Details...

Helix

111 – 149

39

Sequences

	Sequence		Length	Mass (Da)
	O24646-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 1E3C4672B192D8A9	FASTA	168	18,463
	10 20 30 40 50 60 MQEQATSSLA ASSLPSSSER SSSSAPHLEI KEGIESDEEI RRVPEFGGEA VGKETSGRES 70 80 90 100 110 120 GSATGQERTQ ATVGESQRKR GRTPAEKENK RLKRLLRNRV SAQQARERKK AYLSELENRV 130 140 150 160 KDLENKNSEL EERLSTLQNE NQMLRHILKN TTGNKRGGGG GSNADASL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arabidopsis HY5 gene encodes a bZIP protein that regulates stimulus-induced development of root and hypocotyl." Oyama T., Shimura Y., Okada K. Genes Dev. 11:2983-2995(1997) [PubMed: 9367981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta. Tissue: Seedling.
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Targeted destabilization of HY5 during light-regulated development of Arabidopsis." Osterlund M.T., Hardtke C.S., Wei N., Deng X.-W. Nature 405:462-466(2000) [PubMed: 10839542] [Abstract] Cited for: UBIQUITINATION, SUBSEQUENT DEGRADATION.
[4]	"HY5 stability and activity in Arabidopsis is regulated by phosphorylation in its COP1 binding domain." Hardtke C.S., Gohda K., Osterlund M.T., Oyama T., Okada K., Deng X.-W. EMBO J. 19:4997-5006(2000) [PubMed: 10990463] [Abstract] Cited for: PHOSPHORYLATION.
[5]	"Identification of a structural motif that confers specific interaction with the WD40 repeat domain of Arabidopsis COP1." Holm M., Hardtke C.S., Gaudet R., Deng X.-W. EMBO J. 20:118-127(2001) [PubMed: 11226162] [Abstract] Cited for: INTERACTION WITH COP1, MUTAGENESIS OF 43-VAL-PRO-44. Tissue: Etiolated seedling.
[6]	"Biochemical evidence for ubiquitin ligase activity of the Arabidopsis COP1 interacting protein 8 (CIP8)." Hardtke C.S., Okamoto H., Stoop-Myer C., Deng X.-W. Plant J. 30:385-394(2002) [PubMed: 12028569] [Abstract] Cited for: UBIQUITINATION BY CIP8.
[7]	"bZIP transcription factors in Arabidopsis." Jakoby M., Weisshaar B., Droege-Laser W., Vicente-Carbajosa J., Tiedemann J., Kroj T., Parcy F. Trends Plant Sci. 7:106-111(2002) [PubMed: 11906833] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis." Holm M., Ma L.-G., Qu L.-J., Deng X.-W. Genes Dev. 16:1247-1259(2002) [PubMed: 12023303] [Abstract] Cited for: HETERODIMERIZATION.
[9]	"Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis." de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E., Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J., Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H. J. Proteome Res. 7:2458-2470(2008) [PubMed: 18433157] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY.
[10]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed: 19376835] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB005456 mRNA. Translation: BAA21327.1.
AB005295 mRNA. Translation: BAA21116.1.
AL360314 Genomic DNA. Translation: CAB96661.1.

IPI

IPI00535293.

PIR

T50922.

RefSeq

NP_568246.1.

UniGene

At.20257

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2OQQ	X-ray	2.00	A/B	111-150	[»]

DisProt

DP00469.

ModBase

Search...

Protein-protein interaction databases

IntAct

O24646. 7 interactions.

STRING

O24646.

Proteomic databases

PRIDE

O24646.

Genome annotation databases

GeneID

830996.

GenomeReviews

Gene locus AT5G11260 in contig BA000015_GR.

KEGG

ath:AT5G11260.

Organism-specific databases

TAIR

At5g11260.

Phylogenomic databases

eggNOG

KOG1414.

HOGENOM

HBG316354.

InParanoid

O24646.

OMA

RKKAYLS.

Gene expression databases

ArrayExpress

O24646.

Genevestigator

O24646.

GermOnline

AT5G11260. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR011616. bZIP_1.
IPR001630. Leuzip_CREB.
IPR004827. TF_bZIP.
[Graphical view]

Pfam

PF00170. bZIP_1. 1 hit.
[Graphical view]

PRINTS

PR00041. LEUZIPPRCREB.

SMART

SM00338. BRLZ. 1 hit.
[Graphical view]

PROSITE

PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

HY5_ARATH

Accession

Primary (citable) accession number: O24646

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)