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Protein

Transcription factor HY5

Gene

HY5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that promotes photomorphogenesis in light. Acts downstream of the light receptor network and directly affects transcription of light-induced genes. Specifically involved in the blue light specific pathway, suggesting that it participates in transmission of cryptochromes (CRY1 and CRY2) signals to downstream responses. In darkness, its degradation prevents the activation of light-induced genes (Probable). Acts coordinately with SPL7 to regulate the microRNA miR408 and its target genes in response to changes in light and copper conditions (PubMed:25516599). Regulates the abscisic acid (ABA) signaling pathway. Also involved in root gravitropism (PubMed:26474641).Curated2 Publications

GO - Molecular functioni

  • DNA binding Source: TAIR
  • double-stranded DNA binding Source: TAIR
  • regulatory region DNA binding Source: CAFA
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: GO_Central
  • sequence-specific DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • gibberellic acid mediated signaling pathway Source: TAIR
  • positive gravitropism Source: UniProtKB
  • positive regulation of anthocyanin metabolic process Source: TAIR
  • positive regulation of circadian rhythm Source: TAIR
  • red, far-red light phototransduction Source: UniProtKB-KW
  • red or far-red light signaling pathway Source: TAIR
  • regulation of abscisic acid-activated signaling pathway Source: UniProtKB
  • regulation of photomorphogenesis Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to far red light Source: TAIR
  • response to karrikin Source: TAIR
  • response to red light Source: TAIR
  • response to UV-B Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processAbscisic acid signaling pathway, Phytochrome signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor HY5
Alternative name(s):
Protein LONG HYPOCOTYL 5
bZIP transcription factor 56
Short name:
AtbZIP56
Gene namesi
Name:HY5
Synonyms:BZIP56
Ordered Locus Names:At5g11260
ORF Names:F2I11_150
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRilocus:2148007. AT5G11260.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
  • protein-DNA complex Source: CAFA

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Reduced sensitivity to abscisic acid (ABA) leading to impaired ABA-mediated reduction of seed germination. The double mutant shw1 hy5 has altered root growth, hypocotyl length and hook angle similar to the single mutant shw1 in the darkness and far red light (FR), but shorter hypocotyl in WL, red light (RL) and blue light (BL). In addition, shw1 hy5 is recued for gravitropic root growth defect observed in hy5 single mutant.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi43 – 44VP → AA: Abolishes interaction with COP1. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000765601 – 168Transcription factor HY5Add BLAST168

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by CK2. Shows a stronger interaction with COP1 when unphosphorylated. However, phosphorylation does not affect its susceptibility to be ubiquitinated.1 Publication
Ubiquitinated by COP1 and/or CIP8. Ubiquitination takes place in darkness and leads to its subsequent degradation, thereby preventing activation of photomorphogenesis signals (PubMed:10839542, PubMed:12028569). Ubiquitination and subsequent COP1-mediated degradation is triggered by SHW1 in the darkness during seedling development (PubMed:26474641).3 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO24646.

PTM databases

iPTMnetiO24646.

Expressioni

Tissue specificityi

Expressed in root, hypocotyl, cotyledon, leaf, stem and floral organs.

Gene expression databases

ExpressionAtlasiO24646. baseline and differential.
GenevisibleiO24646. AT.

Interactioni

Subunit structurei

Homodimer; homodimerizes via the leucine-zipper domains (PubMed:17261584). Heterodimer; heterodimerizes with HYH via the leucine-zipper domains (PubMed:12023303). Interacts with COP1 WD40 domain (PubMed:11226162). Interacts with BBX21 (PubMed:21632973), BBX24/STO (PubMed:23733077) and BBX25/STH (PubMed:23624715). Interacts with SPL7 (PubMed:25516599). Binds to SHW1 in the nucleus (PubMed:26474641).7 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi16274. 41 interactors.
IntActiO24646. 6 interactors.
MINTiMINT-1536182.
STRINGi3702.AT5G11260.1.

Structurei

Secondary structure

1168
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi111 – 149Combined sources39

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OQQX-ray2.00A/B111-150[»]
DisProtiDP00469.
ProteinModelPortaliO24646.
SMRiO24646.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO24646.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 151bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 46Interaction with COP11 PublicationAdd BLAST12
Regioni90 – 110Basic motifPROSITE-ProRule annotationAdd BLAST21
Regioni116 – 144Leucine-zipperPROSITE-ProRule annotationAdd BLAST29

Sequence similaritiesi

Belongs to the bZIP family.Curated

Phylogenomic databases

eggNOGiKOG1414. Eukaryota.
ENOG4111CH5. LUCA.
HOGENOMiHOG000272717.
InParanoidiO24646.
KOiK16241.
OMAiAGPDRVQ.
OrthoDBiEOG09360JFT.
PhylomeDBiO24646.

Family and domain databases

InterProiView protein in InterPro
IPR004827. bZIP.
IPR001630. Leuzip_CREB.
PfamiView protein in Pfam
PF00170. bZIP_1. 1 hit.
PRINTSiPR00041. LEUZIPPRCREB.
SMARTiView protein in SMART
SM00338. BRLZ. 1 hit.
PROSITEiView protein in PROSITE
PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.

Sequencei

Sequence statusi: Complete.

O24646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQATSSLA ASSLPSSSER SSSSAPHLEI KEGIESDEEI RRVPEFGGEA
60 70 80 90 100
VGKETSGRES GSATGQERTQ ATVGESQRKR GRTPAEKENK RLKRLLRNRV
110 120 130 140 150
SAQQARERKK AYLSELENRV KDLENKNSEL EERLSTLQNE NQMLRHILKN
160
TTGNKRGGGG GSNADASL
Length:168
Mass (Da):18,463
Last modified:January 1, 1998 - v1
Checksum:i1E3C4672B192D8A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005456 mRNA. Translation: BAA21327.1.
AB005295 mRNA. Translation: BAA21116.1.
AL360314 Genomic DNA. Translation: CAB96661.1.
CP002688 Genomic DNA. Translation: AED91653.1.
PIRiT50922.
RefSeqiNP_568246.1. NM_121164.5.
UniGeneiAt.20257.

Genome annotation databases

EnsemblPlantsiAT5G11260.1; AT5G11260.1; AT5G11260.
GeneIDi830996.
GrameneiAT5G11260.1; AT5G11260.1; AT5G11260.
KEGGiath:AT5G11260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005456 mRNA. Translation: BAA21327.1.
AB005295 mRNA. Translation: BAA21116.1.
AL360314 Genomic DNA. Translation: CAB96661.1.
CP002688 Genomic DNA. Translation: AED91653.1.
PIRiT50922.
RefSeqiNP_568246.1. NM_121164.5.
UniGeneiAt.20257.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OQQX-ray2.00A/B111-150[»]
DisProtiDP00469.
ProteinModelPortaliO24646.
SMRiO24646.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16274. 41 interactors.
IntActiO24646. 6 interactors.
MINTiMINT-1536182.
STRINGi3702.AT5G11260.1.

PTM databases

iPTMnetiO24646.

Proteomic databases

PaxDbiO24646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G11260.1; AT5G11260.1; AT5G11260.
GeneIDi830996.
GrameneiAT5G11260.1; AT5G11260.1; AT5G11260.
KEGGiath:AT5G11260.

Organism-specific databases

AraportiAT5G11260.
TAIRilocus:2148007. AT5G11260.

Phylogenomic databases

eggNOGiKOG1414. Eukaryota.
ENOG4111CH5. LUCA.
HOGENOMiHOG000272717.
InParanoidiO24646.
KOiK16241.
OMAiAGPDRVQ.
OrthoDBiEOG09360JFT.
PhylomeDBiO24646.

Miscellaneous databases

EvolutionaryTraceiO24646.
PROiPR:O24646.

Gene expression databases

ExpressionAtlasiO24646. baseline and differential.
GenevisibleiO24646. AT.

Family and domain databases

InterProiView protein in InterPro
IPR004827. bZIP.
IPR001630. Leuzip_CREB.
PfamiView protein in Pfam
PF00170. bZIP_1. 1 hit.
PRINTSiPR00041. LEUZIPPRCREB.
SMARTiView protein in SMART
SM00338. BRLZ. 1 hit.
PROSITEiView protein in PROSITE
PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHY5_ARATH
AccessioniPrimary (citable) accession number: O24646
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 1, 1998
Last modified: June 7, 2017
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.