ID   O24641_ANACO            Unreviewed;       352 AA.
AC   O24641;
DT   01-JAN-1998, integrated into UniProtKB/TrEMBL.
DT   01-JAN-1998, sequence version 1.
DT   27-MAR-2024, entry version 107.
DE   SubName: Full=Bromelain {ECO:0000313|EMBL:BAA21848.1, ECO:0000313|EMBL:OAY85826.1};
DE   SubName: Full=FB31 {ECO:0000313|EMBL:BAA22543.1};
GN   ORFNames=ACMD2_17643 {ECO:0000313|EMBL:OAY85826.1};
OS   Ananas comosus (Pineapple) (Ananas ananas).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Bromeliaceae;
OC   Bromelioideae; Ananas.
OX   NCBI_TaxID=4615 {ECO:0000313|EMBL:BAA22543.1};
RN   [1] {ECO:0000313|EMBL:BAA21848.1}
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Fruit {ECO:0000313|EMBL:BAA21848.1};
RA   Muta E., Aramaki H., Takata Y., Kono A., Okamoto Y., Ota S.;
RT   "Cloning and sequencing of fruit bromelain.";
RL   Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:BAA22543.1}
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Fruit {ECO:0000313|EMBL:BAA22543.1};
RA   Muta E., Okamoto Y., Ota S.;
RT   "Cloning and sequencing of cysteine proteinases in Ananas comosus.";
RL   Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EMBL:OAY85826.1, ECO:0000313|Proteomes:UP000092600}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. MD2 {ECO:0000313|Proteomes:UP000092600};
RC   TISSUE=Leaf {ECO:0000313|EMBL:OAY85826.1};
RX   PubMed=27374615; DOI=10.1093/dnares/dsw026;
RA   Redwan R.M., Saidin A., Kumar S.V.;
RT   "The draft genome of MD-2 pineapple using hybrid error correction of long
RT   reads.";
RL   DNA Res. 0:0-0(2016).
CC   -!- SIMILARITY: Belongs to the peptidase C1 family.
CC       {ECO:0000256|ARBA:ARBA00008455}.
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DR   EMBL; D14058; BAA21848.1; -; mRNA.
DR   EMBL; D38531; BAA22543.1; -; mRNA.
DR   EMBL; LSRQ01000042; OAY85826.1; -; Genomic_DNA.
DR   PIR; T10501; T10501.
DR   AlphaFoldDB; O24641; -.
DR   SMR; O24641; -.
DR   STRING; 4615.O24641; -.
DR   Allergome; 694; Ana c 2.
DR   MEROPS; C01.028; -.
DR   BRENDA; 3.4.22.32; 333.
DR   BRENDA; 3.4.22.33; 333.
DR   Proteomes; UP000092600; Unassembled WGS sequence.
DR   GO; GO:0008234; F:cysteine-type peptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   CDD; cd02248; Peptidase_C1A; 1.
DR   Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR025661; Pept_asp_AS.
DR   InterPro; IPR000169; Pept_cys_AS.
DR   InterPro; IPR025660; Pept_his_AS.
DR   InterPro; IPR013128; Peptidase_C1A.
DR   InterPro; IPR000668; Peptidase_C1A_C.
DR   InterPro; IPR039417; Peptidase_C1A_papain-like.
DR   InterPro; IPR013201; Prot_inhib_I29.
DR   PANTHER; PTHR12411:SF741; CATHEPSIN K; 1.
DR   PANTHER; PTHR12411; CYSTEINE PROTEASE FAMILY C1-RELATED; 1.
DR   Pfam; PF08246; Inhibitor_I29; 1.
DR   Pfam; PF00112; Peptidase_C1; 1.
DR   PRINTS; PR00705; PAPAIN.
DR   SMART; SM00848; Inhibitor_I29; 1.
DR   SMART; SM00645; Pept_C1; 1.
DR   SUPFAM; SSF54001; Cysteine proteinases; 1.
DR   PROSITE; PS00640; THIOL_PROTEASE_ASN; 1.
DR   PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
DR   PROSITE; PS00639; THIOL_PROTEASE_HIS; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW   Reference proteome {ECO:0000313|Proteomes:UP000092600};
KW   Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           25..352
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5008814698"
FT   DOMAIN          37..94
FT                   /note="Cathepsin propeptide inhibitor"
FT                   /evidence="ECO:0000259|SMART:SM00848"
FT   DOMAIN          124..336
FT                   /note="Peptidase C1A papain C-terminal"
FT                   /evidence="ECO:0000259|SMART:SM00645"
SQ   SEQUENCE   352 AA;  38823 MW;  5969AAC09EB3D367 CRC64;
     MASKVQLVFL FLFLCVMWAS PSAASRDEPS DPMMKRFEEW MAEYGRVYKD NDEKMRRFQI
     FKNNVNHIET FNNRNGNSYT LGINKFTDMT NNEFVAQYTG GISRPLNIEK EPVVSFDDVN
     ISAVGQSIDW RDYGAVTEVK DQNPCGSCWA FSAIATVEGI YKIVTGYLVS LSEQEVLDCA
     VSNGCDGGFV DNAYDFIISN NGVASEADYP YQAYQGDCAA NSWPNSAYIT GYSYVRSNDE
     SSMKYAVWNQ PIAAAIDASG DNFQYYNGGV FSGPCGTSLN HAITIIGYGQ DSSGTQYWIV
     KNSWGSSWGE RGYIRMARGV SSSGLCGIAM DPLYPTLQSG ANVAVIKMVS KT
//