Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA mismatch repair protein MSH2

Gene

MSH2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the post-replicative DNA mismatch repair system (MMR). Forms three different heterodimers: MutS alpha (MSH2-MSH6 heterodimer), MutS beta (MSH2-MSH3 heterodimer) and MutS gamma (MSH2-MSH7 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. MutS alpha and MutS beta recognize single base mismatches and trinucleotide insertion-deletion loops (IDL) in the DNA. MutS gamma recognizes specifically the T/G single base mismatch. Plays a role in DNA homologous recombination repair and has a broad range of anti-recombination effects. Can suppress recombination between divergent direct repeats in somatic cells and possesses an anti-recombination meiotic effect. Is involved in a UV-B-induced DNA damage response pathway.8 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi666 – 6738ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • maintenance of DNA repeat elements Source: GO_Central
  • meiotic gene conversion Source: GO_Central
  • meiotic mismatch repair Source: GO_Central
  • mismatch repair Source: TAIR
  • negative regulation of reciprocal meiotic recombination Source: TAIR
  • postreplication repair Source: GO_Central
  • pyrimidine dimer repair Source: TAIR
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-ATH-5358606. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MSH2
Short name:
AtMSH2
Alternative name(s):
MutS protein homolog 2
Gene namesi
Name:MSH2
Ordered Locus Names:At3g18524
ORF Names:MYF24.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G18524.

Subcellular locationi

GO - Cellular componenti

  • MutSalpha complex Source: GO_Central
  • MutSbeta complex Source: GO_Central
  • nuclear chromosome Source: GO_Central
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 937937DNA mismatch repair protein MSH2PRO_0000115187Add
BLAST

Proteomic databases

PaxDbiO24617.
PRIDEiO24617.

Expressioni

Inductioni

By UV-B.1 Publication

Gene expression databases

GenevisibleiO24617. AT.

Interactioni

Subunit structurei

Heterodimer consisting of MSH2-MSH6 (MutS alpha), MSH2-MSH3 (MutS beta) and MSH2-MSH7 (MutS gamma).

Protein-protein interaction databases

BioGridi6716. 6 interactions.
MINTiMINT-8060640.
STRINGi3702.AT3G18524.1.

Structurei

3D structure databases

ProteinModelPortaliO24617.
SMRiO24617. Positions 14-923.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA mismatch repair MutS family.Curated

Phylogenomic databases

eggNOGiKOG0219. Eukaryota.
COG0249. LUCA.
HOGENOMiHOG000196498.
InParanoidiO24617.
KOiK08735.
OMAiNDWYLAY.
OrthoDBiEOG0936014Y.
PhylomeDBiO24617.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR011184. DNA_mismatch_repair_MutS.
IPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR032642. Msh2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11361:SF35. PTHR11361:SF35. 1 hit.
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
PIRSFiPIRSF005813. MSH2. 1 hit.
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O24617-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGNFEEQNK LPELKLDAKQ AQGFLSFYKT LPNDTRAVRF FDRKDYYTAH
60 70 80 90 100
GENSVFIAKT YYHTTTALRQ LGSGSNALSS VSISRNMFET IARDLLLERN
110 120 130 140 150
DHTVELYEGS GSNWRLVKTG SPGNIGSFED VLFANNEMQD TPVVVSIFPS
160 170 180 190 200
FHDGRCVIGM AYVDLTRRVL GLAEFLDDSR FTNLESSLIA LGAKECIFPA
210 220 230 240 250
ESGKSNECKS LYDSLERCAV MITERKKHEF KGRDLDSDLK RLVKGNIEPV
260 270 280 290 300
RDLVSGFDLA TPALGALLSF SELLSNEDNY GNFTIRRYDI GGFMRLDSAA
310 320 330 340 350
MRALNVMESK TDANKNFSLF GLMNRTCTAG MGKRLLHMWL KQPLVDLNEI
360 370 380 390 400
KTRLDIVQCF VEEAGLRQDL RQHLKRISDV ERLLRSLERR RGGLQHIIKL
410 420 430 440 450
YQSTIRLPFI KTAMQQYTGE FASLISERYL KKLEALSDQD HLGKFIDLVE
460 470 480 490 500
CSVDLDQLEN GEYMISSSYD TKLASLKDQK ELLEQQIHEL HKKTAIELDL
510 520 530 540 550
QVDKALKLDK AAQFGHVFRI TKKEEPKIRK KLTTQFIVLE TRKDGVKFTN
560 570 580 590 600
TKLKKLGDQY QSVVDDYRSC QKELVDRVVE TVTSFSEVFE DLAGLLSEMD
610 620 630 640 650
VLLSFADLAA SCPTPYCRPE ITSSDAGDIV LEGSRHPCVE AQDWVNFIPN
660 670 680 690 700
DCRLMRGKSW FQIVTGPNMG GKSTFIRQVG VIVLMAQVGS FVPCDKASIS
710 720 730 740 750
IRDCIFARVG AGDCQLRGVS TFMQEMLETA SILKGASDKS LIIIDELGRG
760 770 780 790 800
TSTYDGFGLA WAICEHLVQV KRAPTLFATH FHELTALAQA NSEVSGNTVG
810 820 830 840 850
VANFHVSAHI DTESRKLTML YKVEPGACDQ SFGIHVAEFA NFPESVVALA
860 870 880 890 900
REKAAELEDF SPSSMIINNE ESGKRKSRED DPDEVSRGAE RAHKFLKEFA
910 920 930
AIPLDKMELK DSLQRVREMK DELEKDAADC HWLRQFL
Length:937
Mass (Da):105,503
Last modified:January 1, 1998 - v1
Checksum:iBDE4D207FCF7C9B4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti781 – 7811F → Y in AAD00647 (Ref. 6) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti404 – 4041T → A in strain: cv. Landsberg erecta.
Natural varianti468 – 4681S → N in strain: cv. Landsberg erecta.
Natural varianti624 – 6241S → L in strain: cv. Landsberg erecta.
Natural varianti737 – 7371S → T in strain: cv. Landsberg erecta.
Natural varianti902 – 9021I → M in strain: cv. Landsberg erecta.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026549 mRNA. Translation: AAB81282.1.
AF109243 Genomic DNA. Translation: AAD04176.1.
AF002706 mRNA. Translation: AAB82649.1.
AF003005 Genomic DNA. Translation: AAB82650.1.
AB026658, AP001303 Genomic DNA. Translation: BAB01119.1.
CP002686 Genomic DNA. Translation: AEE76112.1.
U87911 mRNA. Translation: AAD00647.1.
RefSeqiNP_566804.3. NM_113607.3.
UniGeneiAt.10475.

Genome annotation databases

EnsemblPlantsiAT3G18524.1; AT3G18524.1; AT3G18524.
GeneIDi821383.
GrameneiAT3G18524.1; AT3G18524.1; AT3G18524.
KEGGiath:AT3G18524.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026549 mRNA. Translation: AAB81282.1.
AF109243 Genomic DNA. Translation: AAD04176.1.
AF002706 mRNA. Translation: AAB82649.1.
AF003005 Genomic DNA. Translation: AAB82650.1.
AB026658, AP001303 Genomic DNA. Translation: BAB01119.1.
CP002686 Genomic DNA. Translation: AEE76112.1.
U87911 mRNA. Translation: AAD00647.1.
RefSeqiNP_566804.3. NM_113607.3.
UniGeneiAt.10475.

3D structure databases

ProteinModelPortaliO24617.
SMRiO24617. Positions 14-923.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6716. 6 interactions.
MINTiMINT-8060640.
STRINGi3702.AT3G18524.1.

Proteomic databases

PaxDbiO24617.
PRIDEiO24617.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G18524.1; AT3G18524.1; AT3G18524.
GeneIDi821383.
GrameneiAT3G18524.1; AT3G18524.1; AT3G18524.
KEGGiath:AT3G18524.

Organism-specific databases

TAIRiAT3G18524.

Phylogenomic databases

eggNOGiKOG0219. Eukaryota.
COG0249. LUCA.
HOGENOMiHOG000196498.
InParanoidiO24617.
KOiK08735.
OMAiNDWYLAY.
OrthoDBiEOG0936014Y.
PhylomeDBiO24617.

Enzyme and pathway databases

ReactomeiR-ATH-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-ATH-5358606. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).

Miscellaneous databases

PROiO24617.

Gene expression databases

GenevisibleiO24617. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR011184. DNA_mismatch_repair_MutS.
IPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR032642. Msh2.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11361:SF35. PTHR11361:SF35. 1 hit.
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
PIRSFiPIRSF005813. MSH2. 1 hit.
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSH2_ARATH
AccessioniPrimary (citable) accession number: O24617
Secondary accession number(s): Q9SQ60, Q9ZR93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.