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Protein

DNA mismatch repair protein MSH2

Gene

MSH2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the post-replicative DNA mismatch repair system (MMR). Forms three different heterodimers: MutS alpha (MSH2-MSH6 heterodimer), MutS beta (MSH2-MSH3 heterodimer) and MutS gamma (MSH2-MSH7 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. MutS alpha and MutS beta recognize single base mismatches and trinucleotide insertion-deletion loops (IDL) in the DNA. MutS gamma recognizes specifically the T/G single base mismatch. Plays a role in DNA homologous recombination repair and has a broad range of anti-recombination effects. Can suppress recombination between divergent direct repeats in somatic cells and possesses an anti-recombination meiotic effect. Is involved in a UV-B-induced DNA damage response pathway.8 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi666 – 673ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • maintenance of DNA repeat elements Source: GO_Central
  • meiotic gene conversion Source: GO_Central
  • mismatch repair Source: TAIR
  • negative regulation of reciprocal meiotic recombination Source: TAIR
  • postreplication repair Source: GO_Central
  • pyrimidine dimer repair Source: TAIR

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)
R-ATH-5358606 Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta)

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MSH2
Short name:
AtMSH2
Alternative name(s):
MutS protein homolog 2
Gene namesi
Name:MSH2
Ordered Locus Names:At3g18524
ORF Names:MYF24.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G18524
TAIRilocus:2095097 AT3G18524

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001151871 – 937DNA mismatch repair protein MSH2Add BLAST937

Proteomic databases

PaxDbiO24617
PRIDEiO24617

Expressioni

Inductioni

By UV-B.1 Publication

Gene expression databases

ExpressionAtlasiO24617 baseline and differential
GenevisibleiO24617 AT

Interactioni

Subunit structurei

Heterodimer consisting of MSH2-MSH6 (MutS alpha), MSH2-MSH3 (MutS beta) and MSH2-MSH7 (MutS gamma).

Protein-protein interaction databases

BioGridi6716, 6 interactors
STRINGi3702.AT3G18524.1

Structurei

3D structure databases

ProteinModelPortaliO24617
SMRiO24617
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA mismatch repair MutS family.Curated

Phylogenomic databases

eggNOGiKOG0219 Eukaryota
COG0249 LUCA
HOGENOMiHOG000196498
InParanoidiO24617
KOiK08735
OMAiGDFYTAH
OrthoDBiEOG0936014Y
PhylomeDBiO24617

Family and domain databases

Gene3Di3.30.420.110, 1 hit
3.40.1170.10, 1 hit
InterProiView protein in InterPro
IPR011184 DNA_mismatch_repair_Msh2
IPR007695 DNA_mismatch_repair_MutS-lik_N
IPR000432 DNA_mismatch_repair_MutS_C
IPR007861 DNA_mismatch_repair_MutS_clamp
IPR007696 DNA_mismatch_repair_MutS_core
IPR016151 DNA_mismatch_repair_MutS_N
IPR036187 DNA_mismatch_repair_MutS_sf
IPR007860 DNA_mmatch_repair_MutS_con_dom
IPR032642 Msh2
IPR036678 MutS_con_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR11361:SF35 PTHR11361:SF35, 1 hit
PfamiView protein in Pfam
PF01624 MutS_I, 1 hit
PF05188 MutS_II, 1 hit
PF05192 MutS_III, 1 hit
PF05190 MutS_IV, 1 hit
PF00488 MutS_V, 1 hit
PIRSFiPIRSF005813 MSH2, 1 hit
SMARTiView protein in SMART
SM00534 MUTSac, 1 hit
SM00533 MUTSd, 1 hit
SUPFAMiSSF48334 SSF48334, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00486 DNA_MISMATCH_REPAIR_2, 1 hit

Sequencei

Sequence statusi: Complete.

O24617-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGNFEEQNK LPELKLDAKQ AQGFLSFYKT LPNDTRAVRF FDRKDYYTAH
60 70 80 90 100
GENSVFIAKT YYHTTTALRQ LGSGSNALSS VSISRNMFET IARDLLLERN
110 120 130 140 150
DHTVELYEGS GSNWRLVKTG SPGNIGSFED VLFANNEMQD TPVVVSIFPS
160 170 180 190 200
FHDGRCVIGM AYVDLTRRVL GLAEFLDDSR FTNLESSLIA LGAKECIFPA
210 220 230 240 250
ESGKSNECKS LYDSLERCAV MITERKKHEF KGRDLDSDLK RLVKGNIEPV
260 270 280 290 300
RDLVSGFDLA TPALGALLSF SELLSNEDNY GNFTIRRYDI GGFMRLDSAA
310 320 330 340 350
MRALNVMESK TDANKNFSLF GLMNRTCTAG MGKRLLHMWL KQPLVDLNEI
360 370 380 390 400
KTRLDIVQCF VEEAGLRQDL RQHLKRISDV ERLLRSLERR RGGLQHIIKL
410 420 430 440 450
YQSTIRLPFI KTAMQQYTGE FASLISERYL KKLEALSDQD HLGKFIDLVE
460 470 480 490 500
CSVDLDQLEN GEYMISSSYD TKLASLKDQK ELLEQQIHEL HKKTAIELDL
510 520 530 540 550
QVDKALKLDK AAQFGHVFRI TKKEEPKIRK KLTTQFIVLE TRKDGVKFTN
560 570 580 590 600
TKLKKLGDQY QSVVDDYRSC QKELVDRVVE TVTSFSEVFE DLAGLLSEMD
610 620 630 640 650
VLLSFADLAA SCPTPYCRPE ITSSDAGDIV LEGSRHPCVE AQDWVNFIPN
660 670 680 690 700
DCRLMRGKSW FQIVTGPNMG GKSTFIRQVG VIVLMAQVGS FVPCDKASIS
710 720 730 740 750
IRDCIFARVG AGDCQLRGVS TFMQEMLETA SILKGASDKS LIIIDELGRG
760 770 780 790 800
TSTYDGFGLA WAICEHLVQV KRAPTLFATH FHELTALAQA NSEVSGNTVG
810 820 830 840 850
VANFHVSAHI DTESRKLTML YKVEPGACDQ SFGIHVAEFA NFPESVVALA
860 870 880 890 900
REKAAELEDF SPSSMIINNE ESGKRKSRED DPDEVSRGAE RAHKFLKEFA
910 920 930
AIPLDKMELK DSLQRVREMK DELEKDAADC HWLRQFL
Length:937
Mass (Da):105,503
Last modified:January 1, 1998 - v1
Checksum:iBDE4D207FCF7C9B4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti781F → Y in AAD00647 (Ref. 6) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti404T → A in strain: cv. Landsberg erecta. 1
Natural varianti468S → N in strain: cv. Landsberg erecta. 1
Natural varianti624S → L in strain: cv. Landsberg erecta. 1
Natural varianti737S → T in strain: cv. Landsberg erecta. 1
Natural varianti902I → M in strain: cv. Landsberg erecta. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026549 mRNA Translation: AAB81282.1
AF109243 Genomic DNA Translation: AAD04176.1
AF002706 mRNA Translation: AAB82649.1
AF003005 Genomic DNA Translation: AAB82650.1
AB026658, AP001303 Genomic DNA Translation: BAB01119.1
CP002686 Genomic DNA Translation: AEE76112.1
U87911 mRNA Translation: AAD00647.1
RefSeqiNP_566804.3, NM_113607.4
UniGeneiAt.10475

Genome annotation databases

EnsemblPlantsiAT3G18524.1; AT3G18524.1; AT3G18524
GeneIDi821383
GrameneiAT3G18524.1; AT3G18524.1; AT3G18524
KEGGiath:AT3G18524

Similar proteinsi

Entry informationi

Entry nameiMSH2_ARATH
AccessioniPrimary (citable) accession number: O24617
Secondary accession number(s): Q9SQ60, Q9ZR93
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health