O24617 (MSH2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA mismatch repair protein MSH2
Short name=AtMSH2

Alternative name(s):
MutS protein homolog 2

Gene names

Name:	MSH2
Ordered Locus Names:	At3g18524
ORF Names:	MYF24.25

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	937 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the post-replicative DNA mismatch repair system (MMR). Forms three different heterodimers: MutS alpha (MSH2-MSH6 heterodimer), MutS beta (MSH2-MSH3 heterodimer) and MutS gamma (MSH2-MSH7 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. MutS alpha and MutS beta recognize single base mismatches and trinucleotide insertion-deletion loops (IDL) in the DNA. MutS gamma recognizes specifically the T/G single base mismatch. Plays a role in DNA homologous recombination repair and has a broad range of anti-recombination effects. Can suppress recombination between divergent direct repeats in somatic cells and possesses an anti-recombination meiotic effect. Is involved in a UV-B-induced DNA damage response pathway. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14
Subunit structure	Heterodimer consisting of MSH2-MSH6 (MutS alpha), MSH2-MSH3 (MutS beta) and MSH2-MSH7 (MutS gamma).
Subcellular location	Nucleus Potential.
Induction	By UV-B. Ref.14
Disruption phenotype	No visible phenotype under normal growth conditions. Ref.9
Sequence similarities	Belongs to the DNA mismatch repair MutS family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maintenance of DNA repeat elements Inferred from Biological aspect of Ancestor. Source: RefGenome meiotic gene conversion Inferred from Biological aspect of Ancestor. Source: RefGenome meiotic mismatch repair Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of reciprocal meiotic recombination Inferred from mutant phenotype Ref.11. Source: TAIR postreplication repair Inferred from Biological aspect of Ancestor. Source: RefGenome pyrimidine dimer repair Inferred from mutant phenotype Ref.14. Source: TAIR
Cellular_component	MutSalpha complex Inferred from Biological aspect of Ancestor. Source: RefGenome MutSbeta complex Inferred from Biological aspect of Ancestor. Source: RefGenome nuclear chromosome Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from direct assay PubMed 14506206. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-dependent ATPase activity Inferred from Biological aspect of Ancestor. Source: RefGenome Y-form DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome damaged DNA binding Inferred from direct assay Ref.7. Source: TAIR double-strand/single-strand DNA junction binding Inferred from Biological aspect of Ancestor. Source: RefGenome four-way junction DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome guanine/thymine mispair binding Inferred from Biological aspect of Ancestor. Source: RefGenome loop DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome single base insertion or deletion binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 937	937	DNA mismatch repair protein MSH2		PRO_0000115187
Regions
	Nucleotide binding	666 – 673	8	ATP Potential
Natural variations
	Natural variant	404	1	T → A in strain: cv. Landsberg erecta.
	Natural variant	468	1	S → N in strain: cv. Landsberg erecta.
	Natural variant	624	1	S → L in strain: cv. Landsberg erecta.
	Natural variant	737	1	S → T in strain: cv. Landsberg erecta.
	Natural variant	902	1	I → M in strain: cv. Landsberg erecta.
Experimental info
	Sequence conflict	781	1	F → Y in AAD00647. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	O24617 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: BDE4D207FCF7C9B4	FASTA	937	105,503
	10 20 30 40 50 60 MEGNFEEQNK LPELKLDAKQ AQGFLSFYKT LPNDTRAVRF FDRKDYYTAH GENSVFIAKT 70 80 90 100 110 120 YYHTTTALRQ LGSGSNALSS VSISRNMFET IARDLLLERN DHTVELYEGS GSNWRLVKTG 130 140 150 160 170 180 SPGNIGSFED VLFANNEMQD TPVVVSIFPS FHDGRCVIGM AYVDLTRRVL GLAEFLDDSR 190 200 210 220 230 240 FTNLESSLIA LGAKECIFPA ESGKSNECKS LYDSLERCAV MITERKKHEF KGRDLDSDLK 250 260 270 280 290 300 RLVKGNIEPV RDLVSGFDLA TPALGALLSF SELLSNEDNY GNFTIRRYDI GGFMRLDSAA 310 320 330 340 350 360 MRALNVMESK TDANKNFSLF GLMNRTCTAG MGKRLLHMWL KQPLVDLNEI KTRLDIVQCF 370 380 390 400 410 420 VEEAGLRQDL RQHLKRISDV ERLLRSLERR RGGLQHIIKL YQSTIRLPFI KTAMQQYTGE 430 440 450 460 470 480 FASLISERYL KKLEALSDQD HLGKFIDLVE CSVDLDQLEN GEYMISSSYD TKLASLKDQK 490 500 510 520 530 540 ELLEQQIHEL HKKTAIELDL QVDKALKLDK AAQFGHVFRI TKKEEPKIRK KLTTQFIVLE 550 560 570 580 590 600 TRKDGVKFTN TKLKKLGDQY QSVVDDYRSC QKELVDRVVE TVTSFSEVFE DLAGLLSEMD 610 620 630 640 650 660 VLLSFADLAA SCPTPYCRPE ITSSDAGDIV LEGSRHPCVE AQDWVNFIPN DCRLMRGKSW 670 680 690 700 710 720 FQIVTGPNMG GKSTFIRQVG VIVLMAQVGS FVPCDKASIS IRDCIFARVG AGDCQLRGVS 730 740 750 760 770 780 TFMQEMLETA SILKGASDKS LIIIDELGRG TSTYDGFGLA WAICEHLVQV KRAPTLFATH 790 800 810 820 830 840 FHELTALAQA NSEVSGNTVG VANFHVSAHI DTESRKLTML YKVEPGACDQ SFGIHVAEFA 850 860 870 880 890 900 NFPESVVALA REKAAELEDF SPSSMIINNE ESGKRKSRED DPDEVSRGAE RAHKFLKEFA 910 920 930 AIPLDKMELK DSLQRVREMK DELEKDAADC HWLRQFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA mismatch repair in plants. An Arabidopsis thaliana gene that predicts a protein belonging to the MSH2 subfamily of eukaryotic MutS homologs." Culligan K.M., Hays J.B. Plant Physiol. 115:833-839(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Columbia.
[2]	"Four mismatch repair paralogues coexist in Arabidopsis thaliana: AtMSH2, AtMSH3, AtMSH6-1 and AtMSH6-2." Ade J., Belzile F., Philippe H., Doutriaux M.P. Mol. Gen. Genet. 262:239-249(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia and cv. Landsberg erecta.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	Cerovic G., Radman M. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 665-781.
[7]	"Arabidopsis MutS homologs-AtMSH2, AtMSH3, AtMSH6, and a novel AtMSH7-form three distinct protein heterodimers with different specificities for mismatched DNA." Culligan K.M., Hays J.B. Plant Cell 12:991-1002(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MSH3; MSH6 AND MSH7.
[8]	"Functional analysis of the Arabidopsis thaliana mismatch repair gene MSH2." Ade J., Haffani Y., Beizile F.J. Genome 44:651-657(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Reduction of stability of arabidopsis genomic and transgenic DNA-repeat sequences (microsatellites) by inactivation of AtMSH2 mismatch-repair function." Leonard J.M., Bollmann S.R., Hays J.B. Plant Physiol. 133:328-338(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[10]	"Dissimilar mispair-recognition spectra of Arabidopsis DNA-mismatch-repair proteins MSH2*MSH6 (MutSalpha) and MSH2*MSH7 (MutSgamma)." Wu S.Y., Culligan K., Lamers M., Hays J. Nucleic Acids Res. 31:6027-6034(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MSH6 AND MSH7.
[11]	"The role of AtMSH2 in homologous recombination in Arabidopsis thaliana." Emmanuel E., Yehuda E., Melamed-Bessudo C., Avivi-Ragolsky N., Levy A.A. EMBO Rep. 7:100-105(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"The impact of sequence divergence and DNA mismatch repair on homeologous recombination in Arabidopsis." Li L., Jean M., Belzile F. Plant J. 45:908-916(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"Impact of the loss of AtMSH2 on double-strand break-induced recombination between highly diverged homeologous sequences in Arabidopsis thaliana germinal tissues." Lafleuriel J., Degroote F., Depeiges A., Picard G. Plant Mol. Biol. 63:833-846(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[14]	"Regulation of plant MSH2 and MSH6 genes in the UV-B-induced DNA damage response." Lario L.D., Ramirez-Parra E., Gutierrez C., Casati P., Spampinato C.P. J. Exp. Bot. 62:2925-2937(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION BY UV-B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF026549 mRNA. Translation: AAB81282.1. AF109243 Genomic DNA. Translation: AAD04176.1. AF002706 mRNA. Translation: AAB82649.1. AF003005 Genomic DNA. Translation: AAB82650.1. AB026658, AP001303 Genomic DNA. Translation: BAB01119.1. CP002686 Genomic DNA. Translation: AEE76112.1. U87911 mRNA. Translation: AAD00647.1.
IPI	IPI00526204.
RefSeq	NP_566804.3. NM_113607.3.
UniGene	At.10475.
3D structure databases
ProteinModelPortal	O24617.
SMR	O24617. Positions 14-923.
ModBase	Search...
Proteomic databases
PaxDb	O24617.
PRIDE	O24617.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G18524.1; AT3G18524.1; AT3G18524.
GeneID	821383.
KEGG	ath:AT3G18524.
Organism-specific databases
TAIR	At3g18524.
Phylogenomic databases
eggNOG	COG0249.
HOGENOM	HOG000196498.
InParanoid	O24617.
KO	K08735.
OMA	WAISEHI.
PhylomeDB	O24617.
ProtClustDB	CLSN2680872.
Gene expression databases
Genevestigator	O24617.
GermOnline	AT3G18524. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011184. DNA_mismatch_repair_MSH2. IPR007695. DNA_mismatch_repair_MutS-lik_N. IPR000432. DNA_mismatch_repair_MutS_C. IPR007861. DNA_mismatch_repair_MutS_clamp. IPR007696. DNA_mismatch_repair_MutS_core. IPR007860. DNA_mmatch_repair_MutS_con_dom. [Graphical view]
Pfam	PF01624. MutS_I. 1 hit. PF05188. MutS_II. 1 hit. PF05192. MutS_III. 1 hit. PF05190. MutS_IV. 1 hit. PF00488. MutS_V. 1 hit. [Graphical view]
PIRSF	PIRSF005813. MSH2. 1 hit.
SMART	SM00534. MUTSac. 1 hit. SM00533. MUTSd. 1 hit. [Graphical view]
SUPFAM	SSF48334. DNA_repair_MutS_domIII. 1 hit.
PROSITE	PS00486. DNA_MISMATCH_REPAIR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSH2_ARATH

Accession

Primary (citable) accession number: O24617
Secondary accession number(s): Q9SQ60, Q9ZR93

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program