ID   EIN3_ARATH              Reviewed;         628 AA.
AC   O24606; Q8VYW4;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 65.
DE   RecName: Full=Protein ETHYLENE INSENSITIVE 3;
GN   Name=EIN3; OrderedLocusNames=At3g20770; ORFNames=MOE17.8;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND MUTANTS
RP   EIN3-1; EIN3-2 AND EIN3-3.
RC   STRAIN=cv. Columbia;
RX   MEDLINE=97358539; PubMed=9215635; DOI=10.1016/S0092-8674(00)80300-1;
RA   Chao Q., Rothenberg M., Solano R., Roman G., Terzaghi W., Ecker J.R.;
RT   "Activation of the ethylene gas response pathway in Arabidopsis by the
RT   nuclear protein ETHYLENE-INSENSITIVE3 and related proteins.";
RL   Cell 89:1133-1144(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20277480; PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   CHARACTERIZATION, AND FUNCTION.
RX   MEDLINE=99069218; PubMed=9851977;
RA   Solano R., Stepanova A.N., Chao Q., Ecker J.R.;
RT   "Nuclear events in ethylene signaling: a transcriptional cascade
RT   mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1.";
RL   Genes Dev. 12:3703-3714(1998).
CC   -!- FUNCTION: Probable transcription factor acting as a positive
CC       regulator in the ethylene response pathway. Is required for
CC       ethylene responsiveness in adult plant tissues. Binds a primary
CC       ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1
CC       promoter with consequence to activate the transcription of this
CC       gene.
CC   -!- SUBUNIT: Acts as homodimer to bind the primary ethylene response
CC       element.
CC   -!- INTERACTION:
CC       Q9SKK0:EBF1; NbExp=3; IntAct=EBI-593576, EBI-401198;
CC       Q708Y0:EBF2; NbExp=3; IntAct=EBI-593576, EBI-593623;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- MISCELLANEOUS: Loss-of-function mutations (ein3-1 and ein3-2) in
CC       the gene lead to the suppression of ethylene-mediated effects
CC       including gene expression, the triple response, cell growth
CC       inhibition, and accelerated senescence.
CC   -!- SIMILARITY: Belongs to the EIN3 family.
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DR   EMBL; AF004216; AAC49749.1; -; mRNA.
DR   EMBL; AF004217; AAC49750.1; -; Genomic_DNA.
DR   EMBL; AB025629; BAB02485.1; -; Genomic_DNA.
DR   EMBL; AY069875; AAL47431.2; -; mRNA.
DR   IPI; IPI00540843; -.
DR   RefSeq; NP_188713.1; -.
DR   UniGene; At.24824; -.
DR   SMR; O24606; 178-301.
DR   IntAct; O24606; 2.
DR   PRIDE; O24606; -.
DR   GeneID; 821625; -.
DR   GenomeReviews; BA000014_GR; AT3G20770.
DR   KEGG; ath:AT3G20770; -.
DR   NMPDR; fig|3702.1.peg.14310; -.
DR   GeneFarm; 2364; -.
DR   TAIR; At3g20770; -.
DR   OMA; O24606; FHVNEVK.
DR   ArrayExpress; O24606; -.
DR   GermOnline; AT3G20770; Arabidopsis thaliana.
DR   GO; GO:0005634; C:nucleus; IDA:TAIR.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0003700; F:transcription factor activity; IDA:TAIR.
DR   GO; GO:0009873; P:ethylene mediated signaling pathway; IMP:TAIR.
DR   GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
DR   GO; GO:0010182; P:sugar mediated signaling; TAS:TAIR.
DR   GO; GO:0006350; P:transcription; IEA:UniProtKB-KW.
DR   InterPro; IPR006957; EIN3.
DR   Pfam; PF04873; EIN3; 1.
PE   1: Evidence at protein level;
KW   Activator; Coiled coil; Complete proteome; DNA-binding;
KW   Ethylene signaling pathway; Nucleus; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    628       Protein ETHYLENE INSENSITIVE 3.
FT                                /FTId=PRO_0000113498.
FT   COILED       38     68       Potential.
FT   COMPBIAS    533    583       Asn-rich.
FT   MUTAGEN     245    245       K->N: In ein3-3; suppression of DNA-
FT                                binding.
SQ   SEQUENCE   628 AA;  71421 MW;  8DA269C9F2A3BFD3 CRC64;
     MMFNEMGMCG NMDFFSSGSL GEVDFCPVPQ AEPDSIVEDD YTDDEIDVDE LERRMWRDKM
     RLKRLKEQDK GKEGVDAAKQ RQSQEQARRK KMSRAQDGIL KYMLKMMEVC KAQGFVYGII
     PENGKPVTGA SDNLREWWKD KVRFDRNGPA AITKYQAENN IPGIHEGNNP IGPTPHTLQE
     LQDTTLGSLL SALMQHCDPP QRRFPLEKGV PPPWWPNGKE DWWPQLGLPK DQGPAPYKKP
     HDLKKAWKVG VLTAVIKHMF PDIAKIRKLV RQSKCLQDKM TAKESATWLA IINQEESLAR
     ELYPESCPPL SLSGGSCSLL MNDCSQYDVE GFEKESHYEV EELKPEKVMN SSNFGMVAKM
     HDFPVKEEVP AGNSEFMRKR KPNRDLNTIM DRTVFTCENL GCAHSEISRG FLDRNSRDNH
     QLACPHRDSR LPYGAAPSRF HVNEVKPVVG FPQPRPVNSV AQPIDLTGIV PEDGQKMISE
     LMSMYDRNVQ SNQTSMVMEN QSVSLLQPTV HNHQEHLQFP GNMVEGSFFE DLNIPNRANN
     NNSSNNQTFF QGNNNNNNVF KFDTADHNNF EAAHNNNNNS SGNRFQLVFD STPFDMASFD
     YRDDMSMPGV VGTMDGMQQK QQDVSIWF
//