O24606 (EIN3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein ETHYLENE INSENSITIVE 3

Gene names

Name:	EIN3
Ordered Locus Names:	At3g20770
ORF Names:	MOE17.8

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable transcription factor acting as a positive regulator in the ethylene response pathway. Is required for ethylene responsiveness in adult plant tissues. Binds a primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter with consequence to activate the transcription of this gene. Ref.1 Ref.5
Subunit structure	Acts as homodimer to bind the primary ethylene response element.
Subcellular location	Nucleus.
Post-translational modification	Ubiquitinated. Ref.6
Miscellaneous	Loss-of-function mutations (ein3-1 and ein3-2) in the gene lead to the suppression of ethylene-mediated effects including gene expression, the triple response, cell growth inhibition, and accelerated senescence.
Sequence similarities	Belongs to the EIN3 family.

Ontologies

Keywords
Biological process	Ethylene signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
Ligand	DNA-binding
Molecular function	Activator
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to iron ion Inferred from expression pattern. Source: TAIR defense response to bacterium Inferred from genetic interaction. Source: TAIR ethylene mediated signaling pathway Inferred from mutant phenotype Ref.1. Source: TAIR sugar mediated signaling pathway Traceable author statement. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay Ref.1. Source: TAIR
Molecular function	DNA binding Inferred from physical interaction. Source: TAIR protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.5. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EBF1	Q9SKK0	3	EBI-593576,EBI-401198
EBF2	Q708Y0	3	EBI-593576,EBI-593623

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	Protein ETHYLENE INSENSITIVE 3		PRO_0000113498
Regions
	Coiled coil	38 – 68	31	Potential
	Compositional bias	533 – 583	51	Asn-rich
Experimental info
	Mutagenesis	245	1	K → N in ein3-3; suppression of DNA-binding.

Sequences

	Sequence		Length	Mass (Da)
	O24606 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 8DA269C9F2A3BFD3	FASTA	628	71,421
	10 20 30 40 50 60 MMFNEMGMCG NMDFFSSGSL GEVDFCPVPQ AEPDSIVEDD YTDDEIDVDE LERRMWRDKM 70 80 90 100 110 120 RLKRLKEQDK GKEGVDAAKQ RQSQEQARRK KMSRAQDGIL KYMLKMMEVC KAQGFVYGII 130 140 150 160 170 180 PENGKPVTGA SDNLREWWKD KVRFDRNGPA AITKYQAENN IPGIHEGNNP IGPTPHTLQE 190 200 210 220 230 240 LQDTTLGSLL SALMQHCDPP QRRFPLEKGV PPPWWPNGKE DWWPQLGLPK DQGPAPYKKP 250 260 270 280 290 300 HDLKKAWKVG VLTAVIKHMF PDIAKIRKLV RQSKCLQDKM TAKESATWLA IINQEESLAR 310 320 330 340 350 360 ELYPESCPPL SLSGGSCSLL MNDCSQYDVE GFEKESHYEV EELKPEKVMN SSNFGMVAKM 370 380 390 400 410 420 HDFPVKEEVP AGNSEFMRKR KPNRDLNTIM DRTVFTCENL GCAHSEISRG FLDRNSRDNH 430 440 450 460 470 480 QLACPHRDSR LPYGAAPSRF HVNEVKPVVG FPQPRPVNSV AQPIDLTGIV PEDGQKMISE 490 500 510 520 530 540 LMSMYDRNVQ SNQTSMVMEN QSVSLLQPTV HNHQEHLQFP GNMVEGSFFE DLNIPNRANN 550 560 570 580 590 600 NNSSNNQTFF QGNNNNNNVF KFDTADHNNF EAAHNNNNNS SGNRFQLVFD STPFDMASFD 610 620 YRDDMSMPGV VGTMDGMQQK QQDVSIWF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins." Chao Q., Rothenberg M., Solano R., Roman G., Terzaghi W., Ecker J.R. Cell 89:1133-1144(1997) [PubMed: 9215635] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, MUTANTS EIN3-1; EIN3-2 AND EIN3-3. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1." Solano R., Stepanova A.N., Chao Q., Ecker J.R. Genes Dev. 12:3703-3714(1998) [PubMed: 9851977] [Abstract] Cited for: CHARACTERIZATION, FUNCTION.
[6]	"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed: 19292762] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF004216 mRNA. Translation: AAC49749.1. AF004217 Genomic DNA. Translation: AAC49750.1. AB025629 Genomic DNA. Translation: BAB02485.1. CP002686 Genomic DNA. Translation: AEE76421.1. AY069875 mRNA. Translation: AAL47431.2.
IPI	IPI00540843.
RefSeq	NP_188713.1. NM_112968.3.
UniGene	At.24824.
3D structure databases
ProteinModelPortal	O24606.
SMR	O24606. Positions 178-301.
ModBase	Search...
Protein-protein interaction databases
IntAct	O24606. 2 interactions.
STRING	O24606.
Proteomic databases
PRIDE	O24606.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G20770.1; AT3G20770.1; AT3G20770.
GeneID	821625.
GenomeReviews	Gene locus AT3G20770 in contig BA000014_GR.
KEGG	ath:AT3G20770.
NMPDR	fig|3702.1.peg.14310.
Organism-specific databases
GeneFarm	2364.
TAIR	At3g20770.
Phylogenomic databases
GeneTree	EPGT00050000013227.
HOGENOM	HBG594100.
InParanoid	O24606.
OMA	FHVNEVK.
PhylomeDB	O24606.
ProtClustDB	CLSN2913078.
Gene expression databases
ArrayExpress	O24606.
Genevestigator	O24606.
GermOnline	AT3G20770. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006957. EIN3. IPR023278. Ethylene_insens-like_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.3180.10. Ethylene_insens_like_DNA_bd. 1 hit.
KO	K14514.
Pfam	PF04873. EIN3. 1 hit. [Graphical view]
SUPFAM	SSF116768. SSF116768. 1 hit.
ProtoNet	Search...

Entry information

Entry name

EIN3_ARATH

Accession

Primary (citable) accession number: O24606
Secondary accession number(s): Q8VYW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program