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Protein

Protein ETHYLENE INSENSITIVE 3

Gene

EIN3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable transcription factor acting as a positive regulator in the ethylene response pathway. Is required for ethylene responsiveness in adult plant tissues. Binds a primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter with consequence to activate the transcription of this gene.2 Publications

Miscellaneous

Loss-of-function mutations (ein3-1 and ein3-2) in the gene lead to the suppression of ethylene-mediated effects including gene expression, the triple response, cell growth inhibition, and accelerated senescence.

Enzyme regulationi

Activated by phosphorylation by MPK3 and MPK6.1 Publication

GO - Molecular functioni

  • DNA binding transcription factor activity Source: TAIR
  • transcription regulatory region DNA binding Source: TAIR
  • transcription regulatory region sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cellular response to iron ion Source: TAIR
  • defense response to bacterium Source: TAIR
  • ethylene-activated signaling pathway Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • response to ethylene Source: TAIR
  • response to hypoxia Source: TAIR
  • sugar mediated signaling pathway Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processEthylene signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ETHYLENE INSENSITIVE 3
Gene namesi
Name:EIN3
Ordered Locus Names:At3g20770
ORF Names:MOE17.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G20770
TAIRilocus:2091906 AT3G20770

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi245K → N in ein3-3; suppression of DNA-binding. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001134981 – 628Protein ETHYLENE INSENSITIVE 3Add BLAST628

Proteomic databases

PaxDbiO24606

PTM databases

iPTMnetiO24606

Expressioni

Gene expression databases

ExpressionAtlasiO24606 baseline and differential
GenevisibleiO24606 AT

Interactioni

Subunit structurei

Acts as homodimer to bind the primary ethylene response element. Interacts with TAF12B.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi6957, 9 interactors
DIPiDIP-34865N
IntActiO24606, 6 interactors
STRINGi3702.AT3G20770.1

Structurei

Secondary structure

1628
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi174 – 178Combined sources5
Helixi183 – 193Combined sources11
Helixi194 – 196Combined sources3
Beta strandi197 – 199Combined sources3
Helixi201 – 203Combined sources3
Helixi206 – 208Combined sources3
Helixi223 – 226Combined sources4
Helixi240 – 242Combined sources3
Helixi245 – 259Combined sources15
Helixi263 – 271Combined sources9
Helixi274 – 279Combined sources6
Helixi282 – 302Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZDSX-ray1.78A/B174-306[»]
ProteinModelPortaliO24606
SMRiO24606
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili38 – 68Sequence analysisAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi533 – 583Asn-richAdd BLAST51

Sequence similaritiesi

Belongs to the EIN3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFT4 Eukaryota
ENOG4110EBS LUCA
HOGENOMiHOG000240021
KOiK14514
OMAiDNHQLAC
OrthoDBiEOG093604RP
PhylomeDBiO24606

Family and domain databases

Gene3Di1.10.3180.10, 1 hit
InterProiView protein in InterPro
IPR023278 Ethylene_insens-like_DNA-bd
SUPFAMiSSF116768 SSF116768, 1 hit

Sequencei

Sequence statusi: Complete.

O24606-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMFNEMGMCG NMDFFSSGSL GEVDFCPVPQ AEPDSIVEDD YTDDEIDVDE
60 70 80 90 100
LERRMWRDKM RLKRLKEQDK GKEGVDAAKQ RQSQEQARRK KMSRAQDGIL
110 120 130 140 150
KYMLKMMEVC KAQGFVYGII PENGKPVTGA SDNLREWWKD KVRFDRNGPA
160 170 180 190 200
AITKYQAENN IPGIHEGNNP IGPTPHTLQE LQDTTLGSLL SALMQHCDPP
210 220 230 240 250
QRRFPLEKGV PPPWWPNGKE DWWPQLGLPK DQGPAPYKKP HDLKKAWKVG
260 270 280 290 300
VLTAVIKHMF PDIAKIRKLV RQSKCLQDKM TAKESATWLA IINQEESLAR
310 320 330 340 350
ELYPESCPPL SLSGGSCSLL MNDCSQYDVE GFEKESHYEV EELKPEKVMN
360 370 380 390 400
SSNFGMVAKM HDFPVKEEVP AGNSEFMRKR KPNRDLNTIM DRTVFTCENL
410 420 430 440 450
GCAHSEISRG FLDRNSRDNH QLACPHRDSR LPYGAAPSRF HVNEVKPVVG
460 470 480 490 500
FPQPRPVNSV AQPIDLTGIV PEDGQKMISE LMSMYDRNVQ SNQTSMVMEN
510 520 530 540 550
QSVSLLQPTV HNHQEHLQFP GNMVEGSFFE DLNIPNRANN NNSSNNQTFF
560 570 580 590 600
QGNNNNNNVF KFDTADHNNF EAAHNNNNNS SGNRFQLVFD STPFDMASFD
610 620
YRDDMSMPGV VGTMDGMQQK QQDVSIWF
Length:628
Mass (Da):71,421
Last modified:January 1, 1998 - v1
Checksum:i8DA269C9F2A3BFD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004216 mRNA Translation: AAC49749.1
AF004217 Genomic DNA Translation: AAC49750.1
AB025629 Genomic DNA Translation: BAB02485.1
CP002686 Genomic DNA Translation: AEE76421.1
AY069875 mRNA Translation: AAL47431.2
RefSeqiNP_188713.1, NM_112968.4
UniGeneiAt.24824

Genome annotation databases

EnsemblPlantsiAT3G20770.1; AT3G20770.1; AT3G20770
GeneIDi821625
GrameneiAT3G20770.1; AT3G20770.1; AT3G20770
KEGGiath:AT3G20770

Similar proteinsi

Entry informationi

Entry nameiEIN3_ARATH
AccessioniPrimary (citable) accession number: O24606
Secondary accession number(s): Q8VYW4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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