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Protein

Protein ETHYLENE INSENSITIVE 3

Gene

EIN3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription factor acting as a positive regulator in the ethylene response pathway. Is required for ethylene responsiveness in adult plant tissues. Binds a primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter with consequence to activate the transcription of this gene.2 Publications

Enzyme regulationi

Activated by phosphorylation by MPK3 and MPK6.1 Publication

GO - Molecular functioni

  • transcription factor activity, sequence-specific DNA binding Source: TAIR
  • transcription regulatory region DNA binding Source: TAIR
  • transcription regulatory region sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cellular response to iron ion Source: TAIR
  • defense response to bacterium Source: TAIR
  • ethylene-activated signaling pathway Source: TAIR
  • regulation of transcription, DNA-templated Source: TAIR
  • response to ethylene Source: TAIR
  • response to hypoxia Source: TAIR
  • sugar mediated signaling pathway Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Ethylene signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ETHYLENE INSENSITIVE 3
Gene namesi
Name:EIN3
Ordered Locus Names:At3g20770
ORF Names:MOE17.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G20770.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi245K → N in ein3-3; suppression of DNA-binding. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001134981 – 628Protein ETHYLENE INSENSITIVE 3Add BLAST628

Proteomic databases

PaxDbiO24606.

Expressioni

Gene expression databases

GenevisibleiO24606. AT.

Interactioni

Subunit structurei

Acts as homodimer to bind the primary ethylene response element. Interacts with TAF12B.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
EBF1Q9SKK03EBI-593576,EBI-401198
EBF2Q708Y03EBI-593576,EBI-593623

Protein-protein interaction databases

BioGridi6957. 9 interactors.
DIPiDIP-34865N.
IntActiO24606. 2 interactors.
STRINGi3702.AT3G20770.1.

Structurei

Secondary structure

1628
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi174 – 178Combined sources5
Helixi183 – 193Combined sources11
Helixi194 – 196Combined sources3
Beta strandi197 – 199Combined sources3
Helixi201 – 203Combined sources3
Helixi206 – 208Combined sources3
Helixi223 – 226Combined sources4
Helixi240 – 242Combined sources3
Helixi245 – 259Combined sources15
Helixi263 – 271Combined sources9
Helixi274 – 279Combined sources6
Helixi282 – 302Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZDSX-ray1.78A/B174-306[»]
ProteinModelPortaliO24606.
SMRiO24606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili38 – 68Sequence analysisAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi533 – 583Asn-richAdd BLAST51

Sequence similaritiesi

Belongs to the EIN3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFT4. Eukaryota.
ENOG4110EBS. LUCA.
HOGENOMiHOG000240021.
KOiK14514.
OMAiFEAAHNN.
OrthoDBiEOG093604RP.
PhylomeDBiO24606.

Family and domain databases

Gene3Di1.10.3180.10. 1 hit.
InterProiIPR023278. Ethylene_insens-like_DNA-bd.
[Graphical view]
SUPFAMiSSF116768. SSF116768. 1 hit.

Sequencei

Sequence statusi: Complete.

O24606-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMFNEMGMCG NMDFFSSGSL GEVDFCPVPQ AEPDSIVEDD YTDDEIDVDE
60 70 80 90 100
LERRMWRDKM RLKRLKEQDK GKEGVDAAKQ RQSQEQARRK KMSRAQDGIL
110 120 130 140 150
KYMLKMMEVC KAQGFVYGII PENGKPVTGA SDNLREWWKD KVRFDRNGPA
160 170 180 190 200
AITKYQAENN IPGIHEGNNP IGPTPHTLQE LQDTTLGSLL SALMQHCDPP
210 220 230 240 250
QRRFPLEKGV PPPWWPNGKE DWWPQLGLPK DQGPAPYKKP HDLKKAWKVG
260 270 280 290 300
VLTAVIKHMF PDIAKIRKLV RQSKCLQDKM TAKESATWLA IINQEESLAR
310 320 330 340 350
ELYPESCPPL SLSGGSCSLL MNDCSQYDVE GFEKESHYEV EELKPEKVMN
360 370 380 390 400
SSNFGMVAKM HDFPVKEEVP AGNSEFMRKR KPNRDLNTIM DRTVFTCENL
410 420 430 440 450
GCAHSEISRG FLDRNSRDNH QLACPHRDSR LPYGAAPSRF HVNEVKPVVG
460 470 480 490 500
FPQPRPVNSV AQPIDLTGIV PEDGQKMISE LMSMYDRNVQ SNQTSMVMEN
510 520 530 540 550
QSVSLLQPTV HNHQEHLQFP GNMVEGSFFE DLNIPNRANN NNSSNNQTFF
560 570 580 590 600
QGNNNNNNVF KFDTADHNNF EAAHNNNNNS SGNRFQLVFD STPFDMASFD
610 620
YRDDMSMPGV VGTMDGMQQK QQDVSIWF
Length:628
Mass (Da):71,421
Last modified:January 1, 1998 - v1
Checksum:i8DA269C9F2A3BFD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004216 mRNA. Translation: AAC49749.1.
AF004217 Genomic DNA. Translation: AAC49750.1.
AB025629 Genomic DNA. Translation: BAB02485.1.
CP002686 Genomic DNA. Translation: AEE76421.1.
AY069875 mRNA. Translation: AAL47431.2.
RefSeqiNP_188713.1. NM_112968.4.
UniGeneiAt.24824.

Genome annotation databases

EnsemblPlantsiAT3G20770.1; AT3G20770.1; AT3G20770.
GeneIDi821625.
GrameneiAT3G20770.1; AT3G20770.1; AT3G20770.
KEGGiath:AT3G20770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004216 mRNA. Translation: AAC49749.1.
AF004217 Genomic DNA. Translation: AAC49750.1.
AB025629 Genomic DNA. Translation: BAB02485.1.
CP002686 Genomic DNA. Translation: AEE76421.1.
AY069875 mRNA. Translation: AAL47431.2.
RefSeqiNP_188713.1. NM_112968.4.
UniGeneiAt.24824.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZDSX-ray1.78A/B174-306[»]
ProteinModelPortaliO24606.
SMRiO24606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6957. 9 interactors.
DIPiDIP-34865N.
IntActiO24606. 2 interactors.
STRINGi3702.AT3G20770.1.

Proteomic databases

PaxDbiO24606.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G20770.1; AT3G20770.1; AT3G20770.
GeneIDi821625.
GrameneiAT3G20770.1; AT3G20770.1; AT3G20770.
KEGGiath:AT3G20770.

Organism-specific databases

TAIRiAT3G20770.

Phylogenomic databases

eggNOGiENOG410IFT4. Eukaryota.
ENOG4110EBS. LUCA.
HOGENOMiHOG000240021.
KOiK14514.
OMAiFEAAHNN.
OrthoDBiEOG093604RP.
PhylomeDBiO24606.

Miscellaneous databases

PROiO24606.

Gene expression databases

GenevisibleiO24606. AT.

Family and domain databases

Gene3Di1.10.3180.10. 1 hit.
InterProiIPR023278. Ethylene_insens-like_DNA-bd.
[Graphical view]
SUPFAMiSSF116768. SSF116768. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEIN3_ARATH
AccessioniPrimary (citable) accession number: O24606
Secondary accession number(s): Q8VYW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Loss-of-function mutations (ein3-1 and ein3-2) in the gene lead to the suppression of ethylene-mediated effects including gene expression, the triple response, cell growth inhibition, and accelerated senescence.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.