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Protein

Endochitinase CHI

Gene

CHI

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.By similarity

GO - Molecular functioni

GO - Biological processi

  • cell wall macromolecule catabolic process Source: InterPro
  • chitin catabolic process Source: UniProtKB-KW
  • leaf senescence Source: UniProtKB
  • polysaccharide catabolic process Source: UniProtKB-KW
  • response to amitrole Source: UniProtKB
  • response to silver ion Source: UniProtKB
  • response to virus Source: UniProtKB
  • systemic acquired resistance Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Enzyme and pathway databases

BioCyciARA:AT2G43570-MONOMER.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase CHICurated (EC:3.2.1.14)
Gene namesi
Name:CHIImported
Synonyms:LSC2221 Publication
Ordered Locus Names:At2g43570Imported
ORF Names:F18O19.32Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G43570.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • plant-type cell wall Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Chaini32 – 277246Endochitinase CHISequence analysisPRO_0000433910Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 ↔ 42PROSITE-ProRule annotation
Disulfide bondi36 ↔ 48PROSITE-ProRule annotation
Disulfide bondi41 ↔ 55PROSITE-ProRule annotation
Disulfide bondi59 ↔ 64PROSITE-ProRule annotation
Glycosylationi274 – 2741N-linked (GlcNAc...)PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO24603.
PRIDEiO24603.

Expressioni

Inductioni

Accumulates during senescence and in response to 3-amino-1,2,4-triazole (3-AT) and silver nitrate (PubMed:12947053). Induced by viral infection (e.g. cucumber mosaic cucumovirus, oil seed rape tobamovirus, turnip vein clearing tobamovirus, potato virus X potexvirus, and turnip mosaic potyvirus) (PubMed:12535341). Induced by pathogens (PubMed:12920300).3 Publications

Gene expression databases

GenevisibleiO24603. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G43570.1-P.

Structurei

3D structure databases

ProteinModelPortaliO24603.
SMRiO24603. Positions 36-277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 6635Chitin-binding type-1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni75 – 277203CatalyticBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
HOGENOMiHOG000231411.
InParanoidiO24603.
KOiK01183.
OMAiRECSGGD.
PhylomeDBiO24603.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 2 hits.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24603-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKPTSRNDR FALFFITLIF LILTVSKPVA SQNCGCASDF CCSKYGYCGT
60 70 80 90 100
TDEFCGEGCQ AGPCRSSGGG GDPAVSLEGT VTPDFFNSIL NQRGDCPGKG
110 120 130 140 150
FYTHDTFMAA ANSYPSFGAS ISKREIAAFF AHVAQETGFM CYIEEIDGPA
160 170 180 190 200
KAASGEYCDT EKPEFPCAQG KGYYGRGAIQ LSWNYNYGLC GKALDENLLA
210 220 230 240 250
SPEKVAQDQV LAFKTAFWFW TTNVRTSFKS GFGATIRAVN SRECSGGDST
260 270
AKAANRIKYF QDYCGKLGVA PGDNLTC
Length:277
Mass (Da):29,775
Last modified:January 1, 1998 - v1
Checksum:i0D0910C34F5E0AA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002333 Genomic DNA. Translation: AAB64049.1.
AC002335 Genomic DNA. Translation: AAM14808.1.
CP002685 Genomic DNA. Translation: AEC10291.1.
AY099810 mRNA. Translation: AAM20661.1.
BT003417 mRNA. Translation: AAO30080.1.
AK226201 mRNA. Translation: BAE98366.1.
PIRiG84867.
RefSeqiNP_181885.1. NM_129919.4.
UniGeneiAt.36876.

Genome annotation databases

EnsemblPlantsiAT2G43570.1; AT2G43570.1; AT2G43570.
GeneIDi818959.
GrameneiAT2G43570.1; AT2G43570.1; AT2G43570.
KEGGiath:AT2G43570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002333 Genomic DNA. Translation: AAB64049.1.
AC002335 Genomic DNA. Translation: AAM14808.1.
CP002685 Genomic DNA. Translation: AEC10291.1.
AY099810 mRNA. Translation: AAM20661.1.
BT003417 mRNA. Translation: AAO30080.1.
AK226201 mRNA. Translation: BAE98366.1.
PIRiG84867.
RefSeqiNP_181885.1. NM_129919.4.
UniGeneiAt.36876.

3D structure databases

ProteinModelPortaliO24603.
SMRiO24603. Positions 36-277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G43570.1-P.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Proteomic databases

PaxDbiO24603.
PRIDEiO24603.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G43570.1; AT2G43570.1; AT2G43570.
GeneIDi818959.
GrameneiAT2G43570.1; AT2G43570.1; AT2G43570.
KEGGiath:AT2G43570.

Organism-specific databases

TAIRiAT2G43570.

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
HOGENOMiHOG000231411.
InParanoidiO24603.
KOiK01183.
OMAiRECSGGD.
PhylomeDBiO24603.

Enzyme and pathway databases

BioCyciARA:AT2G43570-MONOMER.

Miscellaneous databases

PROiO24603.

Gene expression databases

GenevisibleiO24603. AT.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 2 hits.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Expression pattern of the Arabidopsis thaliana AtEP3/AtchitIV endochitinase gene."
    Passarinho P.A., Van Hengel A.J., Fransz P.F., de Vries S.C.
    Planta 212:556-567(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  6. "Expression of senescence-enhanced genes in response to oxidative stress."
    Navabpour S., Morris K., Allen R., Harrison E., A-H-Mackerness S., Buchanan-Wollaston V.
    J. Exp. Bot. 54:2285-2292(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY SENESCENCE.
    Strain: cv. Columbia.
  7. "Diverse RNA viruses elicit the expression of common sets of genes in susceptible Arabidopsis thaliana plants."
    Whitham S.A., Quan S., Chang H.S., Cooper B., Estes B., Zhu T., Wang X., Hou Y.M.
    Plant J. 33:271-283(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY VIRUS.
    Strain: cv. Columbia.
  8. "Loss of a callose synthase results in salicylic acid-dependent disease resistance."
    Nishimura M.T., Stein M., Hou B.-H., Vogel J.P., Edwards H., Somerville S.C.
    Science 301:969-972(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY PATHOGENS.
    Strain: cv. Columbia.

Entry informationi

Entry nameiCHI_ARATH
AccessioniPrimary (citable) accession number: O24603
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.