Reviewed, UniProtKB/Swiss-Prot O24594 (HMDH_MAIZE)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase
      Short name=HMG-CoA reductase
    EC=1.1.1.34

Gene names

Name:

HMGR

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 579	579	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114444
Regions
	Transmembrane	36 – 56	21	Potential
	Transmembrane	80 – 100	21	Potential
	Transmembrane	524 – 544	21	Potential
	Region	101 – 153	53	Linker By similarity
	Region	154 – 579	426	Catalytic By similarity
Sites
	Active site	247	1	Charge relay system By similarity
	Active site	379	1	Charge relay system By similarity
	Active site	455	1	Charge relay system By similarity
	Active site	553	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	311	1	N-linked (GlcNAc...) Potential
	Glycosylation	557	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O24594-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: ECA693F232EB2B6A	FASTA	579	60,931
	10 20 30 40 50 60 MEVRGGVGQG SAARHPPAPE PSRAAARVQA GDALPLPIRH TNLIFSALFA ASLAYLMRRW 70 80 90 100 110 120 REKIRSSTPL HAVGLAEMLA IFGLVASLIY LLSFFGIAFV QSIVSSGDDD EDFLVGSGSS 130 140 150 160 170 180 GSAAAPSRQH AQAPAPCELL GSPAAAPEKM PEDDEEIVAS VVAGKVPSYA LEARLGDCRR 190 200 210 220 230 240 AAGIRREALR RITGRDIEGL PLDGFDYASI LGQCCELPVG YVQLPVGVAG PLLLDGRRFY 250 260 270 280 290 300 LPMATTEGCL VASTNRGCKA IAESGGATSV VLRDAMTRAP VARFPTARRA AELKAFLEDP 310 320 330 340 350 360 ANFDTLSVVF NRSSRFARLQ GVQCAMAGRN LYMRFSCSTG DAMGMNMVSK GVQNVLDFLQ 370 380 390 400 410 420 DDFHDMDVIS ISGNFCSDKK PSAVNWIEGR GKSVVCEAVI GEEVVKKVLK TDVQSLVELN 430 440 450 460 470 480 TIKNLAGSAV AGALGGFNAH ASNIVTAIFI ATGQDPAQNV ESSHCITMLE PVNAGRDLHI 490 500 510 520 530 540 SVTMPSIEVG TVGGGTQLAS QSACLDLLGV RGASRDRPGS NARLLATVVA GGVLAGELSL 550 560 570 LSALAAGQLV KSHMKYNRSS KDVSSTTATE KTRQREVDV

References

[1]	Mayer M.J., Steel J., Bailey J.A., Hargreaves J.A. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. LG 21-11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09238 Genomic DNA. Translation: CAA70440.1.
PIR	T04357.
3D structure databases
SMR	O24594. Positions 152-550.
ModBase	Search...
Organism-specific databases
Gramene	O24594.
Enzyme and pathway databases
BRENDA	1.1.1.34. 289.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_MAIZE

Accession

Primary (citable) accession number: O24594

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)