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Protein

Pectate lyase

Gene
N/A
Organism
Zinnia violacea (Garden zinnia) (Zinnia elegans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the degradation of pectin. May assist in the removal and modification of an existing pectin matrix in order to allow the deposition of newly synthesized walls polymers for a specialized function or to create an architecture that is extensible.

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactori

Ca2+Note: Binds 1 Ca2+ ion. Required for its activity.

Kineticsi

    1. Vmax=0.12 µmol/min/mg enzyme

    pH dependencei

    Optimum pH is 10.

    Pathwayi: pectin degradation

    This protein is involved in step 2 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
    Proteins known to be involved in the 5 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. Pectate lyase
    3. no protein annotated in this organism
    4. no protein annotated in this organism
    5. no protein annotated in this organism
    This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi199CalciumBy similarity1
    Metal bindingi223CalciumBy similarity1
    Metal bindingi227CalciumBy similarity1
    Active sitei279Sequence analysis1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionLyase
    LigandCalcium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00545; UER00824.

    Protein family/group databases

    CAZyiPL1. Polysaccharide Lyase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pectate lyase (EC:4.2.2.2)
    Alternative name(s):
    ZePel
    OrganismiZinnia violacea (Garden zinnia) (Zinnia elegans)
    Taxonomic identifieri34245 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeZinnia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 20Sequence analysisAdd BLAST20
    ChainiPRO_000002489321 – 401Pectate lyaseAdd BLAST381

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed in sites of vascular differentiation and in new primordia on the flank of the shoot meristem.

    Inductioni

    Up-regulated by auxin.

    Structurei

    3D structure databases

    ProteinModelPortaliO24554.
    SMRiO24554.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the polysaccharide lyase 1 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.160.20.10. 1 hit.
    InterProiView protein in InterPro
    IPR018082. AmbAllergen.
    IPR002022. Pec_lyase.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    PfamiView protein in Pfam
    PF00544. Pec_lyase_C. 1 hit.
    PRINTSiPR00807. AMBALLERGEN.
    SMARTiView protein in SMART
    SM00656. Amb_all. 1 hit.
    SUPFAMiSSF51126. SSF51126. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O24554-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MATTILPLIL FISSLAIASS SPSRTPHAIV NEVHKSINAS RRNLGYLSCG
    60 70 80 90 100
    TGNPIDDCWR CDPNWANNRQ RLADCAIGFG KNAMGGRNGR IYVVTDPGND
    110 120 130 140 150
    DPVNPVPGTL RYAVIQDEPL WIIFKRDMVI QLRQELVMNS HKTIDGRGVN
    160 170 180 190 200
    VHIGNGPCIT IHYASNIIIH GIHIHDCKQA GNGNIRNSPH HSGWWTQSDG
    210 220 230 240 250
    DGISIFASKD IWIDHNSLSN CHDGLIDAIH GSTAITISNN YMTHHDKVML
    260 270 280 290 300
    LGHSDSYTQD KNMQVTIAFN HFGEGLVQRM PRCRHGYFHV VNNDYTHWEM
    310 320 330 340 350
    YAIGGSASPT IYSQGNRFLA PNTRFDKEVT KHENAPESEW KNWNWRSEGD
    360 370 380 390 400
    LMLNGAYFRE SGGRAASSFA RASSLSGRPS TLVASMTRSA GALVCRKGSR

    C
    Length:401
    Mass (Da):44,407
    Last modified:January 1, 1998 - v1
    Checksum:iB5EA3B57A9830F02
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Y09541 mRNA. Translation: CAA70735.1.

    Similar proteinsi

    Entry informationi

    Entry nameiPLY_ZINVI
    AccessioniPrimary (citable) accession number: O24554
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: January 1, 1998
    Last modified: August 30, 2017
    This is version 81 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families