Reviewed, UniProtKB/Swiss-Prot O24554 (PEL_ZINEL)

Names and origin

Protein names	Recommended name:     Pectate lyase     EC=4.2.2.2 Alternative name(s):     ZePel
Organism	Zinnia elegans (Zinnia)
Taxonomic identifier	34245 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Heliantheae › Zinnia

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the degradation of pectin. May assist in the removal and modification of an existing pectin matrix in order to allow the deposition of newly synthesized walls polymers for a specialized function or to create an architecture that is extensible.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Tissue specificity	Expressed in sites of vascular differentiation and in new primordia on the flank of the shoot meristem.
Induction	Up-regulated by auxin.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.
Biophysicochemical properties	Kinetic parameters: Vmax=0.12 µmol/min/mg enzyme pH dependence: Optimum pH is 10.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 401	381	Pectate lyase		PRO_0000024893
Sites
	Active site	279	1	Potential
	Metal binding	199	1	Calcium By similarity
	Metal binding	223	1	Calcium By similarity
	Metal binding	227	1	Calcium By similarity
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O24554-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B5EA3B57A9830F02	FASTA	401	44,407
	10 20 30 40 50 60 MATTILPLIL FISSLAIASS SPSRTPHAIV NEVHKSINAS RRNLGYLSCG TGNPIDDCWR 70 80 90 100 110 120 CDPNWANNRQ RLADCAIGFG KNAMGGRNGR IYVVTDPGND DPVNPVPGTL RYAVIQDEPL 130 140 150 160 170 180 WIIFKRDMVI QLRQELVMNS HKTIDGRGVN VHIGNGPCIT IHYASNIIIH GIHIHDCKQA 190 200 210 220 230 240 GNGNIRNSPH HSGWWTQSDG DGISIFASKD IWIDHNSLSN CHDGLIDAIH GSTAITISNN 250 260 270 280 290 300 YMTHHDKVML LGHSDSYTQD KNMQVTIAFN HFGEGLVQRM PRCRHGYFHV VNNDYTHWEM 310 320 330 340 350 360 YAIGGSASPT IYSQGNRFLA PNTRFDKEVT KHENAPESEW KNWNWRSEGD LMLNGAYFRE 370 380 390 400 SGGRAASSFA RASSLSGRPS TLVASMTRSA GALVCRKGSR C

References

[1]	"A pectate lyase from Zinnia elegans is auxin inducible." Domingo C., Roberts K., Stacey N.J., Connerton I., Ruiz-Teran F., McCann M.C. Plant J. 13:17-28(1998) [PubMed: 9680962] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Envy.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y09541 mRNA. Translation: CAA70735.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Enzyme and pathway databases
BRENDA	4.2.2.2. 228834.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. SM00710. PbH1. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEL_ZINEL

Accession

Primary (citable) accession number: O24554

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	January 1, 1998
Last modified:	September 22, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)