O24540 (4CL_VANPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-coumarate--CoA ligase
Short name=4CL
EC=6.2.1.12

Alternative name(s):
4-coumaroyl-CoA synthase

Gene names

Name:

4CL

Organism

Vanilla planifolia (Vanilla)

Taxonomic identifier

51239 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Asparagales › Orchidaceae › Vanilloideae › Vanillinae › Vanilla

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	4-coumarate--CoA ligase		PRO_0000193042

Sequences

	Sequence		Length	Mass (Da)
	O24540 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9A2D250BC84EA1CB	FASTA	553	60,096
	10 20 30 40 50 60 MAAAVAIEEQ KKDIIFRSKL PDIYIPKNLP LHSYCFENIS KFSSRPCLIN GATDEIFTYA 70 80 90 100 110 120 DVELISRRVG SGLSKLGIKQ GDTIMILLPN SPEFVFAFLG ASFIGSISTM ANPFFTSTEV 130 140 150 160 170 180 IKQAKASNAK LIITQGCYVD KVKDYACENG VKIISIDTTT TADDAANILH FSELTGADEN 190 200 210 220 230 240 EMPKVEISPD GVVALPYSSG TTGLPKGVML THKGLVTSVA QQVDGENPNL YMHSDDVLLC 250 260 270 280 290 300 VLPLFHIYSL NSVLLCGLRA GSGILIMQKF EIVPFLELIQ KYKVTIGPFV PPIVLAIAKS 310 320 330 340 350 360 TVVDNYDLSS VRTVMSGAAP LGKELEDAVR AKFPNAKLGQ GYGMTEAGPV LAMCLAFAKE 370 380 390 400 410 420 PFDIKSGACG TVVRNAEMKI VDPETGSSLP RNHPGEICIR GDQIMKGYLN DPEATARTID 430 440 450 460 470 480 KEGWLHTGDI GYIDDDDELF IVDRLKELIK YKGFQVAPAE LEALLLTHPC ISDAAVVPMK 490 500 510 520 530 540 DEAAGEVPVA FVVKSNGHNI TEDEIKQFIS KQVIFYKRIN RVFFVEAIPK APSGKILRKD 550 LRARLAAAAL PTN

References

[1]	"Isolation and characterization of a cDNA from cell suspension cultures of Vanilla planifolia encoding 4-coumarate: coenzyme A ligase." Brodelius P., Xue Z.T. Plant Physiol. Biochem. 35:497-506(1997) Cited for: NUCLEOTIDE SEQUENCE.
+	Additional computationally mapped references.

Cross-references

3D structure databases
ProteinModelPortal	O24540.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL_VANPL

Accession

Primary (citable) accession number: O24540

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 1, 1998
Last modified:	September 21, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program