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Protein

Isoflavone-7-O-methyltransferase 8

Gene
N/A
Organism
Medicago sativa (Alfalfa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transfers a methyl group to 7-hydroxyls of the isoflavones daidzein, genistein and 6,7,4'-trihydroxyisoflavone. Can also methylate (+)6a-hydroxymaackiain with lower efficiency.

Catalytic activityi

S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 7-methoxyisoflavone.

Pathwayi: medicarpin biosynthesis

This protein is involved in the pathway medicarpin biosynthesis, which is part of Phytoalexin biosynthesis.
View all proteins of this organism that are known to be involved in the pathway medicarpin biosynthesis and in Phytoalexin biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei196S-adenosyl-L-methionine; via carbonyl oxygen1
Binding sitei219S-adenosyl-L-methionine1
Binding sitei239S-adenosyl-L-methionine1
Binding sitei240S-adenosyl-L-methionine; via amide nitrogen1
Binding sitei253S-adenosyl-L-methionine1
Active sitei257Proton acceptor1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.150. 3078.
2.1.1.270. 3078.
UniPathwayiUPA00902.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoflavone-7-O-methyltransferase 8 (EC:2.1.1.150)
Alternative name(s):
7-IOMT-8
Isoflavone-O-methyltransferase 8
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002044371 – 352Isoflavone-7-O-methyltransferase 8Add BLAST352

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1352
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 24Combined sources13
Helixi27 – 38Combined sources12
Helixi41 – 48Combined sources8
Helixi54 – 61Combined sources8
Helixi65 – 67Combined sources3
Helixi68 – 80Combined sources13
Beta strandi83 – 96Combined sources14
Helixi98 – 101Combined sources4
Beta strandi107 – 109Combined sources3
Helixi112 – 118Combined sources7
Helixi121 – 125Combined sources5
Helixi126 – 129Combined sources4
Helixi130 – 134Combined sources5
Helixi141 – 146Combined sources6
Helixi150 – 156Combined sources7
Helixi158 – 170Combined sources13
Helixi172 – 180Combined sources9
Helixi183 – 186Combined sources4
Beta strandi190 – 195Combined sources6
Helixi201 – 209Combined sources9
Beta strandi214 – 219Combined sources6
Helixi221 – 224Combined sources4
Beta strandi233 – 237Combined sources5
Turni240 – 242Combined sources3
Beta strandi248 – 254Combined sources7
Helixi256 – 258Combined sources3
Helixi261 – 275Combined sources15
Helixi277 – 279Combined sources3
Beta strandi283 – 288Combined sources6
Turni293 – 295Combined sources3
Helixi298 – 310Combined sources13
Helixi311 – 314Combined sources4
Helixi321 – 330Combined sources10
Beta strandi335 – 342Combined sources8
Beta strandi345 – 351Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FP2X-ray1.40A1-352[»]
1FPXX-ray1.65A1-352[»]
ProteinModelPortaliO24529.
SMRiO24529.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO24529.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 127Substrate binding10

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR016461. O-MeTrfase_COMT.
IPR001077. O_MeTrfase_2.
IPR012967. Plant_MeTrfase_dimerisation.
IPR029063. SAM-dependent_MTases.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08100. Dimerisation. 1 hit.
PF00891. Methyltransf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005739. O-mtase. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51683. SAM_OMT_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O24529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSINGRKP SEIFKAQALL YKHIYAFIDS MSLKWAVEMN IPNIIQNHGK
60 70 80 90 100
PISLSNLVSI LQVPSSKIGN VRRLMRYLAH NGFFEIITKE EESYALTVAS
110 120 130 140 150
ELLVRGSDLC LAPMVECVLD PTLSGSYHEL KKWIYEEDLT LFGVTLGSGF
160 170 180 190 200
WDFLDKNPEY NTSFNDAMAS DSKLINLALR DCDFVFDGLE SIVDVGGGTG
210 220 230 240 250
TTAKIICETF PKLKCIVFDR PQVVENLSGS NNLTYVGGDM FTSIPNADAV
260 270 280 290 300
LLKYILHNWT DKDCLRILKK CKEAVTNDGK RGKVTIIDMV IDKKKDENQV
310 320 330 340 350
TQIKLLMDVN MACLNGKERN EEEWKKLFIE AGFQHYKISP LTGFLSLIEI

YP
Length:352
Mass (Da):39,604
Last modified:January 1, 1998 - v1
Checksum:i31B95228986C1296
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97125 mRNA. Translation: AAC49928.1.
PIRiT09707.

Genome annotation databases

KEGGiag:AAC49928.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97125 mRNA. Translation: AAC49928.1.
PIRiT09707.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FP2X-ray1.40A1-352[»]
1FPXX-ray1.65A1-352[»]
ProteinModelPortaliO24529.
SMRiO24529.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAC49928.

Enzyme and pathway databases

UniPathwayiUPA00902.
BRENDAi2.1.1.150. 3078.
2.1.1.270. 3078.

Miscellaneous databases

EvolutionaryTraceiO24529.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR016461. O-MeTrfase_COMT.
IPR001077. O_MeTrfase_2.
IPR012967. Plant_MeTrfase_dimerisation.
IPR029063. SAM-dependent_MTases.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08100. Dimerisation. 1 hit.
PF00891. Methyltransf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005739. O-mtase. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51683. SAM_OMT_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei7OMT8_MEDSA
AccessioniPrimary (citable) accession number: O24529
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.